FastQCFastQC Report
Thu 26 May 2016
SRR1043284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043284_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2368256
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900163.800940438871473No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC173660.7332822127337585No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA125200.5286590638849854No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT83790.353804656253378Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCTT77790.3284695573451519Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT66570.281092922386769Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC55880.23595422116527942No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC47590.2009495595070803No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC39010.16472036806831694No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35770.15103941465787482No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31890.13465605069722192No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG27070.11430352124094693No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA26500.11189668684466543No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT25590.10805419684358447Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA206.3128164E-446.00000418
ACTCGAT206.3128164E-446.00000419
CAACCCG650.046.022
CGTTTTT208250.045.2158471
TATACGG1300.044.230771
TAAACGG1150.044.01
ACTACGG1000.043.6999971
TAGTAGG5500.043.0727271
TCGGCGT18300.042.732244
CGGTCTA6000.042.5530
CCGGTAT1200.042.16666842
AATACGG1150.042.01
ACGATGG1100.041.818181
GTCGGCG18950.041.7519763
GCGCGAC23650.041.5264248
CGGGATA3500.041.3999985
TATGCGG3850.041.220781
GACGGTC6250.041.21628
TCACGTT5100.041.03921545
CACTTAC50950.040.98920436
Produced by FastQC (version 0.11.5)