FastQCFastQC Report
Thu 26 May 2016
SRR1043283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043283_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2328775
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT854873.670899936661979No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC166780.7161705188350098No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG104620.4492490687163852No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC79100.3396635570203218No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT76310.3276830093074685Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC65870.2828525727045335No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC54000.23188156863587078No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC46750.20074932099494372No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG37330.1602988695773529No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33280.1429077519296626No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC30960.1329454326845659No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC25620.11001492200835203No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA24690.10602140610406759No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC23310.10009554379448422No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGA207.0347794E-445.018
TATACGA207.0347794E-445.032
TAATGCG207.0347794E-445.039
CGTTTTT198500.044.1725461
ACTACGG950.042.631581
TAGTAGG6250.042.481
TACGGGT1700.042.352943
CCGGTAT700.041.78571742
CGGTCTA6050.041.65289330
TCACGAC6350.041.4566924
CTTGCGG7250.041.275861
CGGGTAT1750.041.142865
ATAGCGG4000.041.06251
GCGCGAC23700.041.0126578
GACGGTC6200.041.00806428
TATACGG1650.040.909091
TAGGGTA10750.040.8139534
ACGATGG1600.040.781251
TACGGGA4150.040.662653
CACTTAC48000.040.6406336
Produced by FastQC (version 0.11.5)