##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043283_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2328775
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30788032334596	33.0	31.0	34.0	31.0	34.0
2	32.59044819701345	34.0	31.0	34.0	31.0	34.0
3	32.71307919399684	34.0	31.0	34.0	31.0	34.0
4	36.18146750974225	37.0	37.0	37.0	35.0	37.0
5	36.17480091464397	37.0	37.0	37.0	35.0	37.0
6	36.32129123680905	37.0	37.0	37.0	35.0	37.0
7	36.273946602827664	37.0	37.0	37.0	35.0	37.0
8	36.172650857210336	37.0	37.0	37.0	35.0	37.0
9	37.765991562087365	39.0	38.0	39.0	35.0	39.0
10	37.57894085946474	39.0	37.0	39.0	35.0	39.0
11	37.33337870768967	39.0	37.0	39.0	35.0	39.0
12	37.36332578286868	39.0	37.0	39.0	35.0	39.0
13	37.36958830286309	39.0	37.0	39.0	35.0	39.0
14	38.74287855202843	40.0	38.0	41.0	35.0	41.0
15	38.809362003628515	41.0	38.0	41.0	35.0	41.0
16	38.75131689407521	41.0	38.0	41.0	35.0	41.0
17	38.71711608034267	40.0	38.0	41.0	34.0	41.0
18	38.54857940333437	40.0	38.0	41.0	34.0	41.0
19	38.36499661839379	40.0	37.0	41.0	34.0	41.0
20	38.165669074942834	40.0	35.0	41.0	34.0	41.0
21	38.091656342927074	40.0	35.0	41.0	34.0	41.0
22	38.05052227029232	40.0	35.0	41.0	34.0	41.0
23	38.02772358858198	40.0	35.0	41.0	34.0	41.0
24	37.92974331998583	40.0	35.0	41.0	34.0	41.0
25	37.8996068748591	40.0	35.0	41.0	34.0	41.0
26	37.861292739745146	40.0	35.0	41.0	34.0	41.0
27	37.855536923919225	40.0	35.0	41.0	34.0	41.0
28	37.75445373640648	40.0	35.0	41.0	34.0	41.0
29	37.73617803351547	40.0	35.0	41.0	34.0	41.0
30	37.62804650513682	40.0	35.0	41.0	34.0	41.0
31	37.500652918379835	40.0	35.0	41.0	33.0	41.0
32	37.32992624877886	40.0	35.0	41.0	33.0	41.0
33	37.02975770523129	40.0	35.0	41.0	33.0	41.0
34	36.79535463924166	40.0	35.0	41.0	32.0	41.0
35	36.6392940494466	39.0	35.0	41.0	31.0	41.0
36	36.54822170454423	39.0	35.0	41.0	31.0	41.0
37	36.47635473585898	39.0	35.0	41.0	31.0	41.0
38	36.329650137948065	39.0	35.0	41.0	31.0	41.0
39	36.224887762879625	39.0	35.0	41.0	31.0	41.0
40	36.10863307962341	39.0	35.0	41.0	31.0	41.0
41	36.02636450494359	38.0	35.0	41.0	30.0	41.0
42	35.91774173116767	38.0	35.0	41.0	30.0	41.0
43	35.797106203905486	38.0	35.0	40.0	30.0	41.0
44	35.68922759820077	38.0	35.0	40.0	30.0	41.0
45	35.62605361187749	38.0	35.0	40.0	29.0	41.0
46	35.50726154308596	37.0	35.0	40.0	29.0	41.0
47	35.331934171399126	37.0	35.0	40.0	29.0	41.0
48	35.24003821751779	37.0	35.0	40.0	28.0	41.0
49	35.151929662590845	36.0	35.0	40.0	28.0	41.0
50	34.975458771242394	36.0	35.0	40.0	28.0	41.0
51	34.52473338987236	36.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	7.0
14	15.0
15	26.0
16	78.0
17	171.0
18	344.0
19	758.0
20	1342.0
21	2273.0
22	3674.0
23	5498.0
24	8893.0
25	15969.0
26	25704.0
27	32911.0
28	35322.0
29	35408.0
30	37108.0
31	42798.0
32	51098.0
33	66527.0
34	142686.0
35	219080.0
36	198038.0
37	219420.0
38	369177.0
39	814301.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.821021781838095	17.959485137035568	23.77065195220663	26.44884112891971
2	33.47008620412019	20.26443087030735	25.245590492855683	21.019892432716773
3	26.906034288413437	21.1890800957585	32.04633337269595	19.85855224313212
4	23.52700454101405	24.23299803544782	29.709611276314803	22.530386147223325
5	21.553177099548044	32.65974600380028	26.804221103369798	18.982855793281875
6	79.90338267973506	2.146450387006044	15.024422711511418	2.925744221747485
7	79.85962576891285	2.050262477053386	13.26555807237711	4.824553681656665
8	69.73808118001953	4.017519940741377	19.023392126761923	7.221006752477161
9	38.91913130293824	19.566252643557235	23.363785681313136	18.150830372191386
10	27.3855997251774	17.5398653798671	35.009951583987295	20.064583310968214
11	24.4705478202059	14.780174125881635	38.022994922223056	22.726283131689407
12	21.073311075565478	16.695472941782697	40.59129800002147	21.63991798263035
13	16.825068974031414	20.679670642290475	40.33459651533531	22.160663868342798
14	16.49648420306814	20.027095790705413	40.402658049833065	23.073761956393383
15	19.280351257635452	15.641614153363893	41.76199933441402	23.31603525458664
16	18.92475657802922	20.35293233567004	36.12628957284409	24.596021513456645
17	19.779111335358717	21.640519157067555	35.692842803619925	22.887526703953796
18	20.703846442872326	26.251999441766593	33.51792251290915	19.526231602451933
19	21.567991755322005	21.699305428819873	32.234157443291004	24.498545372567122
20	21.202434756470677	25.822632070509172	33.1230367897285	19.851896383291646
21	19.118592392996316	20.4452555528121	33.985249755772884	26.4509022984187
22	19.36361391718822	23.268156004766453	31.855503429914872	25.512726648130457
23	20.174512350914107	21.35891187426866	36.105677877854234	22.360897896962996
24	23.958347199708	19.702161007396594	32.30449485244388	24.034996940451524
25	18.76402829813958	21.66538201414907	33.863683696363964	25.706905991347384
26	19.085012506575346	19.940183143498192	34.560702515270904	26.414101834655558
27	23.230926130691028	21.051239385513842	34.39641012978927	21.32142435400586
28	21.457504481970137	23.312041738682353	30.960097046730578	24.270356732616936
29	23.56839969511868	21.003145430537515	33.52191602881343	21.906538845530378
30	27.53082629279342	20.995501926978775	29.51938250797093	21.954289272256876
31	25.936468744296892	23.116960633809622	28.022243454176554	22.924327167716932
32	27.733894429474727	19.60361134072635	29.30489205698275	23.35760217281618
33	23.49840581421563	23.86379104894204	30.6251140621142	22.01268907472813
34	22.67548389174566	21.633004476602505	31.51270517761484	24.178806454036994
35	25.383087674850515	25.39180470418997	29.826754409507146	19.398353211452374
36	22.579854215198978	24.287275498921108	30.36437612049253	22.76849416538738
37	23.326212278987878	25.90898648431042	29.74971819948256	21.01508303721914
38	22.03342959281167	22.105184055995107	29.035522968084077	26.825863383109144
39	24.573821000311323	19.59450784210583	34.07581239063456	21.75585876694829
40	21.028523579993774	19.043445588345804	35.513735762364334	24.414295069296088
41	22.567143669955232	23.58076671211259	28.91597513714292	24.936114480789257
42	21.524921900999452	18.675913302057946	32.77860677824178	27.020558018700818
43	23.16350871166171	20.89085463387403	33.31202026816674	22.63361638629752
44	22.503590943736516	21.584481111313888	32.417987997981776	23.493939946967828
45	24.141404815836655	21.336110186686135	30.915824843533617	23.606660153943597
46	24.385739283528892	20.444697319406124	30.705628495668325	24.46393490139666
47	20.70363173771618	22.388251333855784	33.35676482270722	23.55135210572082
48	22.071775933699048	20.099752015544652	31.08861955319857	26.73985249755773
49	21.978422131807495	17.8429002372492	34.86425266502775	25.314424965915556
50	20.774269734087664	18.691415014331568	35.94413371837125	24.59018153320952
51	19.989865916629988	17.163530182177325	34.152204485190715	28.694399416001975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	642.0
1	3731.0
2	6820.0
3	57654.5
4	108489.0
5	62194.5
6	15900.0
7	14398.5
8	12897.0
9	12733.5
10	12570.0
11	12041.5
12	11513.0
13	10908.5
14	10304.0
15	9618.0
16	8932.0
17	8375.0
18	7818.0
19	7069.5
20	6321.0
21	6527.5
22	6734.0
23	6728.5
24	6723.0
25	7139.0
26	9401.5
27	11248.0
28	11320.0
29	11392.0
30	14051.5
31	16711.0
32	19206.5
33	21702.0
34	25718.0
35	29734.0
36	33795.0
37	37856.0
38	41911.0
39	45966.0
40	52691.0
41	59416.0
42	68062.5
43	76709.0
44	92121.0
45	107533.0
46	135785.5
47	164038.0
48	209975.5
49	255913.0
50	264110.0
51	272307.0
52	229785.0
53	187263.0
54	164112.5
55	140962.0
56	130042.0
57	119122.0
58	114717.5
59	110313.0
60	103961.0
61	97609.0
62	92755.0
63	87901.0
64	86112.5
65	84324.0
66	68304.0
67	52284.0
68	44166.5
69	36049.0
70	31167.0
71	26285.0
72	24275.5
73	22266.0
74	19000.5
75	11589.0
76	7443.0
77	5598.0
78	3753.0
79	3017.0
80	2281.0
81	1500.0
82	719.0
83	596.5
84	474.0
85	309.0
86	144.0
87	103.0
88	62.0
89	45.0
90	28.0
91	17.5
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2328775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.191005875809637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5663502803642	19.005857065790796
2	11.332124402496504	5.935994736223835
3	4.3932897959630735	3.4519403658061014
4	2.1802322571158963	2.2840990342700858
5	1.275824932221868	1.6707569148163692
6	0.8676667544179703	1.363503903792344
7	0.5978142407587091	1.0960149404647819
8	0.4564462014433191	0.9563828115194369
9	0.3862768332601696	0.9105280928645617
>10	4.969207198855688	31.45626908025092
>50	0.7798695938067105	13.404974775735202
>100	0.17979072647816252	8.250298392480826
>500	0.008134421517218242	1.46051393240634
>1k	0.005810301083727315	2.6590214655783435
>5k	6.64034409568836E-4	1.1968987982281485
>10k+	4.98025807176627E-4	4.896945689771929
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85487	3.670899936661979	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16678	0.7161705188350098	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10462	0.4492490687163852	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	7910	0.3396635570203218	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	7631	0.3276830093074685	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	6587	0.2828525727045335	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5400	0.23188156863587078	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4675	0.20074932099494372	No Hit
CGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTG	3733	0.1602988695773529	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3328	0.1429077519296626	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3096	0.1329454326845659	No Hit
TCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	2562	0.11001492200835203	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2469	0.10602140610406759	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC	2331	0.10009554379448422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.339620615989093	0.0
2	0.0	0.0	0.0	1.160352545866389	0.0
3	0.0	0.0	0.0	1.917789395712338	0.0
4	0.0	0.0	0.0	2.42277592296379	0.0
5	0.0	0.0	0.0	5.033848267866153	0.0
6	0.0	0.0	0.0	5.745080568109843	0.0
7	0.0	0.0	0.0	8.11954783094116	0.0
8	0.0	0.0	0.0	10.776438256164722	0.0
9	0.0	0.0	0.0	13.132999108973602	0.0
10	0.0	0.0	0.0	14.515227963199536	0.0
11	0.0	0.0	0.0	15.766658436302349	0.0
12	0.0	0.0	0.0	16.679928288477846	0.0
13	0.0	0.0	0.0	17.105731554143272	0.0
14	0.0	0.0	0.0	17.39231999656472	0.0
15	0.0	0.0	0.0	18.275788773067386	0.0
16	0.0	0.0	0.0	19.269573058797008	0.0
17	0.0	0.0	0.0	20.46685489152022	0.0
18	0.0	0.0	0.0	21.1288338289444	0.0
19	0.0	0.0	0.0	21.853936082275016	0.0
20	4.294103122886496E-5	0.0	0.0	22.645296346791767	0.0
21	4.294103122886496E-5	0.0	0.0	23.422529012034225	0.0
22	4.294103122886496E-5	0.0	0.0	24.221962190422	0.0
23	4.294103122886496E-5	0.0	0.0	24.87840173481766	0.0
24	4.294103122886496E-5	0.0	0.0	25.415164625178473	0.0
25	4.294103122886496E-5	0.0	0.0	25.939088147201854	0.0
26	8.588206245772992E-5	0.0	0.0	26.456956983821968	0.0
27	8.588206245772992E-5	0.0	0.0	27.016478620734077	0.0
28	8.588206245772992E-5	0.0	0.0	27.569301456774486	0.0
29	8.588206245772992E-5	0.0	0.0	28.146171270303057	0.0
30	8.588206245772992E-5	0.0	0.0	28.701184098936135	0.0
31	8.588206245772992E-5	0.0	0.0	29.213599424590182	0.0
32	8.588206245772992E-5	0.0	0.0	29.7743234103767	0.0
33	8.588206245772992E-5	0.0	0.0	30.30176809696085	0.0
34	8.588206245772992E-5	0.0	0.0	30.91157368144196	0.0
35	8.588206245772992E-5	0.0	0.0	31.417676675505362	0.0
36	8.588206245772992E-5	0.0	0.0	31.91948556644588	0.0
37	8.588206245772992E-5	0.0	0.0	32.48755246857253	0.0
38	8.588206245772992E-5	0.0	0.0	33.092162188274955	0.0
39	8.588206245772992E-5	0.0	0.0	33.782611029403874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGA	20	7.0347794E-4	45.0	18
TATACGA	20	7.0347794E-4	45.0	32
TAATGCG	20	7.0347794E-4	45.0	39
CGTTTTT	19850	0.0	44.172546	1
ACTACGG	95	0.0	42.63158	1
TAGTAGG	625	0.0	42.48	1
TACGGGT	170	0.0	42.35294	3
CCGGTAT	70	0.0	41.785717	42
CGGTCTA	605	0.0	41.652893	30
TCACGAC	635	0.0	41.45669	24
CTTGCGG	725	0.0	41.27586	1
CGGGTAT	175	0.0	41.14286	5
ATAGCGG	400	0.0	41.0625	1
GCGCGAC	2370	0.0	41.012657	8
GACGGTC	620	0.0	41.008064	28
TATACGG	165	0.0	40.90909	1
TAGGGTA	1075	0.0	40.813953	4
ACGATGG	160	0.0	40.78125	1
TACGGGA	415	0.0	40.66265	3
CACTTAC	4800	0.0	40.64063	36
>>END_MODULE