FastQCFastQC Report
Thu 26 May 2016
SRR1043282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043282_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2061628
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1027244.982664185779393No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC163510.7931110753249374No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA112770.5469948991767671No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT90570.4393130089424474TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT74330.3605403108611253TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC61410.2978713909589897TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT50780.2463101975720159TruSeq Adapter, Index 20 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT44930.21793456433459382No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC40490.19639818628772987No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC40410.19601014344003864No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39680.19246925245485605No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC29990.14546756252825438No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT29970.14537055181633157No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG29160.14144161798345772No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA27890.13528143777635926No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG24870.12063282027601488No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23820.11553975790006732No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT23270.11287196332219003TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT264450.045.4259761
TACGGGT2250.044.977783
TCACGAC4900.044.12244824
CGACGGT4600.043.527
CTAGCGG3650.042.2191771
CGGGTAC5350.042.130845
TACGCGG1750.042.0571441
CGTTCAG14600.041.74657438
ACGGGTA2150.041.7209324
ACCCGCA44600.041.5650231
TACGGGA5150.041.533983
CCCGCAC44600.041.51345432
GCACTTA44300.041.4830735
TCGGCGT16600.041.427714
CGCACTT44500.041.434
GTCGGCG16500.041.43
ATAACGG1000.041.3999981
GTTTTTT291550.041.3534552
CCGCACT44400.041.33783733
GTTGATC18450.041.26287515
Produced by FastQC (version 0.11.5)