##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043282_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2061628
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33937451373381	33.0	31.0	34.0	31.0	34.0
2	32.66832716668574	34.0	31.0	34.0	31.0	34.0
3	32.78439563296579	34.0	31.0	34.0	31.0	34.0
4	36.20989771190535	37.0	37.0	37.0	35.0	37.0
5	36.1575255089667	37.0	37.0	37.0	35.0	37.0
6	36.31409788768876	37.0	37.0	37.0	35.0	37.0
7	36.26226118387992	37.0	37.0	37.0	35.0	37.0
8	36.259499288911485	37.0	37.0	37.0	35.0	37.0
9	37.80369348883504	39.0	38.0	39.0	35.0	39.0
10	37.68472828269697	39.0	37.0	39.0	35.0	39.0
11	37.41357655212288	39.0	37.0	39.0	35.0	39.0
12	37.40588505782809	39.0	37.0	39.0	35.0	39.0
13	37.385916857939456	39.0	37.0	39.0	35.0	39.0
14	38.803315632112096	41.0	38.0	41.0	35.0	41.0
15	38.86243929554701	41.0	38.0	41.0	35.0	41.0
16	38.756991562008274	41.0	38.0	41.0	35.0	41.0
17	38.76061200177724	41.0	38.0	41.0	34.0	41.0
18	38.52348774851719	40.0	38.0	41.0	34.0	41.0
19	38.257185098378564	40.0	37.0	41.0	34.0	41.0
20	37.987172758616005	40.0	35.0	41.0	34.0	41.0
21	37.922994352036355	40.0	35.0	41.0	34.0	41.0
22	37.868957445281104	40.0	35.0	41.0	34.0	41.0
23	37.87823554976941	40.0	35.0	41.0	34.0	41.0
24	37.77494727467807	40.0	35.0	41.0	34.0	41.0
25	37.67703581829505	40.0	35.0	41.0	34.0	41.0
26	37.589830464079846	40.0	35.0	41.0	34.0	41.0
27	37.60308843302477	40.0	35.0	41.0	34.0	41.0
28	37.54058976692206	39.0	35.0	41.0	34.0	41.0
29	37.48833688715908	39.0	35.0	41.0	34.0	41.0
30	37.34830774514122	39.0	35.0	41.0	33.0	41.0
31	37.25848552697189	40.0	35.0	41.0	33.0	41.0
32	36.977248077732746	39.0	35.0	41.0	33.0	41.0
33	36.62078900752221	39.0	35.0	41.0	31.0	41.0
34	36.3668605587429	39.0	35.0	41.0	31.0	41.0
35	36.16633747698421	39.0	35.0	41.0	30.0	41.0
36	36.11417869761179	39.0	35.0	41.0	30.0	41.0
37	36.07148816372304	39.0	35.0	41.0	30.0	41.0
38	36.01607613012629	39.0	35.0	41.0	30.0	41.0
39	35.978733311732285	39.0	35.0	41.0	29.0	41.0
40	35.89316016274517	39.0	35.0	41.0	29.0	41.0
41	35.876646514308106	39.0	35.0	41.0	29.0	41.0
42	35.79095258698465	39.0	35.0	41.0	29.0	41.0
43	35.67336929843793	39.0	35.0	41.0	28.0	41.0
44	35.479723306047454	38.0	35.0	41.0	27.0	41.0
45	35.37688855603436	38.0	35.0	41.0	26.0	41.0
46	35.34272477867006	38.0	35.0	41.0	26.0	41.0
47	35.24962796391978	38.0	35.0	40.0	26.0	41.0
48	35.16265349519894	38.0	35.0	40.0	26.0	41.0
49	35.1106426571622	37.0	35.0	40.0	26.0	41.0
50	35.00104238009961	37.0	35.0	40.0	26.0	41.0
51	34.87010120157468	37.0	35.0	40.0	26.0	41.0
52	34.47445950481852	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	3.0
14	14.0
15	23.0
16	81.0
17	198.0
18	403.0
19	822.0
20	1465.0
21	2456.0
22	3994.0
23	6270.0
24	11590.0
25	21426.0
26	31694.0
27	36683.0
28	36234.0
29	34186.0
30	34880.0
31	38418.0
32	45098.0
33	58115.0
34	119239.0
35	182314.0
36	183689.0
37	169822.0
38	279167.0
39	761095.0
40	2243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.206010007625043	17.367488218049036	23.57612527575295	30.850376498572974
2	35.66167126174072	19.23387730473199	23.903972976696085	21.200478456831203
3	25.047826280977947	20.26325796894493	35.22027252249194	19.46864322758519
4	22.033024386552764	23.238430987549645	33.8156059192056	20.912938706691993
5	20.035379806638247	31.211838411197363	30.45462129928387	18.29816048288052
6	76.45292943246793	1.7544387251240283	18.79728059572338	2.9953512466846592
7	76.78795592609336	1.5657043850782002	16.85468959482506	4.79165009400338
8	68.14410747234709	2.982206295219118	22.046217843374265	6.827468389059519
9	34.46717836583515	18.02851920909107	27.171730302459995	20.33257212261378
10	27.130258223112996	19.226892533473546	36.643807709247255	16.999041534166203
11	25.86921597882838	15.29339919713935	37.773933997792035	21.063450826240235
12	21.70493415882982	17.055938316708932	40.06712171157939	21.17200581288186
13	17.216830582432912	20.819856928602057	39.88459605709663	22.078716431868408
14	16.516898295909836	21.167058266573797	40.472286949925014	21.84375648759136
15	19.10562914357003	16.400339925534578	42.322814785208585	22.171216145686806
16	18.017799525423598	21.166767234438026	38.20116917310009	22.61426406703828
17	18.67955809680505	21.83269726643216	37.81429045395193	21.673454182810868
18	19.898061143911512	26.920036010376265	35.12107906955086	18.060823776161364
19	19.861003051956995	22.33002268110445	34.596493644828264	23.21248062211029
20	19.125128296666517	26.277534065311492	35.74849584891164	18.848841789110356
21	16.879766863857107	20.739823091265738	36.5914704301649	25.78893961471226
22	17.4772558385897	24.075827452867347	34.33645643151917	24.11046027702379
23	18.179613392910845	22.576429889388386	38.71052391605081	20.53343280164996
24	22.980285483123048	20.39790883709379	34.59562054842096	22.026185131362205
25	18.017702514711676	21.973459809432157	36.08163063365456	23.9272070422016
26	17.391546874605893	20.560692811700267	36.60015288888199	25.447607424811846
27	21.292056568886338	21.56926467820577	37.01026567353567	20.128413079372223
28	20.037950590504202	22.77268255960823	33.62735663271938	23.56201021716818
29	22.542573150927325	21.05336171220026	35.005830343786556	21.39823479308585
30	22.72631143930913	21.8796504510028	34.32985970310842	21.064178406579654
31	23.561137120760876	20.702959020735072	31.52697770887861	24.20892614962544
32	21.606080243380475	21.967833188140634	32.201153651386186	24.224932917092705
33	18.345647226366737	22.94662276608583	34.035335181710764	24.67239482583667
34	18.3278457607289	21.952651011724715	35.14028719051158	24.579216037034808
35	19.206956832173407	26.15263277371087	30.723777519513707	23.916632874602016
36	18.22472337395495	25.373685262326667	31.40052424588723	25.00106711783115
37	18.532392846818148	26.01798190556201	33.208658400060536	22.240966847559307
38	16.633311150217207	22.52748798522333	30.927257487771797	29.911943376787665
39	20.675553494616874	19.98192690436878	34.63442483319008	24.708094767824264
40	17.169052806810928	19.148459372883952	37.269672317217264	26.41281550308785
41	19.115475730830198	23.701899663760873	31.563550747273517	25.619073858135415
42	18.162442496900507	19.060179625034195	34.40227819955879	28.375099678506498
43	21.421032310387712	21.713034553275374	33.151179553246266	23.714753583090644
44	20.119827631367055	22.575217255489353	34.10047787476693	23.20447723837666
45	21.021348177265732	21.52721053458723	33.1508885211105	24.30055276703654
46	21.452609297118588	20.76611299419682	32.86397934059879	24.917298368085806
47	17.810972687604163	22.899136022599613	34.2430351159375	25.046856173858718
48	19.27195400916169	20.83241981579606	31.43670924143444	28.45891693360781
49	19.384971488551766	18.173647234127593	36.16030632102397	26.281074956296674
50	18.598990700553156	18.784766213885337	37.024720269612175	25.591522815949336
51	17.544969315511818	17.353712696956	36.1980919933179	28.90322599421428
52	15.665774814855057	18.531956298614492	39.0462294846597	26.75603940187076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	445.0
1	2341.0
2	4237.0
3	66556.0
4	128875.0
5	73349.0
6	17823.0
7	16775.5
8	15728.0
9	15539.0
10	15350.0
11	15061.5
12	14773.0
13	14197.5
14	12723.5
15	11825.0
16	11150.5
17	10476.0
18	9681.5
19	8887.0
20	8434.5
21	7982.0
22	7727.0
23	7472.0
24	7390.5
25	7309.0
26	7983.0
27	8657.0
28	9259.0
29	9861.0
30	10996.5
31	12132.0
32	14385.0
33	16638.0
34	19189.5
35	21741.0
36	24948.5
37	28156.0
38	30336.5
39	36950.0
40	41383.0
41	49198.0
42	57013.0
43	72278.0
44	87543.0
45	110620.0
46	133697.0
47	169645.5
48	205594.0
49	225236.0
50	244878.0
51	229486.0
52	214094.0
53	181685.0
54	149276.0
55	132477.0
56	115678.0
57	103190.5
58	90703.0
59	83778.0
60	76853.0
61	71277.5
62	65702.0
63	64272.0
64	50192.0
65	37542.0
66	30652.0
67	23762.0
68	20038.0
69	16314.0
70	14512.5
71	12711.0
72	14366.5
73	16022.0
74	12388.5
75	8755.0
76	6214.0
77	3673.0
78	2671.0
79	1669.0
80	1225.0
81	781.0
82	566.0
83	351.0
84	257.5
85	164.0
86	130.0
87	96.0
88	53.0
89	9.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2061628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.791565434089495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35755076769992	23.203769554163532
2	10.425146589051527	6.4201316671351
3	3.781628889285577	3.4932682017564014
4	1.8798378912717777	2.3153260573830305
5	1.0940042950321358	1.6843052417828477
6	0.7613910834217437	1.4066654019667748
7	0.5797982049977628	1.2497026057429372
8	0.494950799710964	1.2192247948764057
9	0.40320940643872544	1.1173903939798562
>10	4.733588794111411	31.6622111144446
>50	0.34420168000618917	7.00989913184346
>100	0.13279276610997845	7.263152561777949
>500	0.00587009087878387	1.1836635766639403
>1k	0.004918184249791891	3.049826692311451
>5k	6.34604419327986E-4	1.3536167579769345
>10k+	4.7595331449598946E-4	6.367846246194715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102724	4.982664185779393	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	16351	0.7931110753249374	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11277	0.5469948991767671	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	9057	0.4393130089424474	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	7433	0.3605403108611253	TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	6141	0.2978713909589897	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	5078	0.2463101975720159	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	4493	0.21793456433459382	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4049	0.19639818628772987	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4041	0.19601014344003864	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3968	0.19246925245485605	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2999	0.14546756252825438	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	2997	0.14537055181633157	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	2916	0.14144161798345772	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2789	0.13528143777635926	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	2487	0.12063282027601488	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2382	0.11553975790006732	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2327	0.11287196332219003	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3733942301908977	0.0
2	0.0	0.0	0.0	1.2577923854351998	0.0
3	0.0	0.0	0.0	2.150872999396593	0.0
4	0.0	0.0	0.0	2.6959761896908656	0.0
5	0.0	0.0	0.0	5.456513008166362	0.0
6	0.0	0.0	0.0	6.413184143793158	0.0
7	0.0	0.0	0.0	9.004049227115658	0.0
8	0.0	0.0	0.0	11.688432636731747	0.0
9	0.0	0.0	0.0	14.405751183045632	0.0
10	0.0	0.0	0.0	15.963597700458084	0.0
11	0.0	0.0	0.0	17.187339326008377	0.0
12	0.0	0.0	0.0	18.09928852343876	0.0
13	0.0	0.0	0.0	18.552959117745782	0.0
14	0.0	0.0	0.0	18.871639306412213	0.0
15	0.0	0.0	0.0	19.7402247156131	0.0
16	0.0	0.0	0.0	20.65561779331674	0.0
17	0.0	0.0	0.0	21.77575197853347	0.0
18	0.0	0.0	0.0	22.360338528580325	0.0
19	0.0	0.0	0.0	23.020981476774665	0.0
20	0.0	0.0	0.0	23.781011899333926	0.0
21	0.0	0.0	0.0	24.513685301130952	0.0
22	0.0	0.0	0.0	25.290741103632662	0.0
23	0.0	0.0	0.0	25.941391948498953	0.0
24	0.0	0.0	0.0	26.500222154530302	0.0
25	4.850535596140526E-5	0.0	0.0	27.01893843118157	0.0
26	4.850535596140526E-5	0.0	0.0	27.563459557204308	0.0
27	4.850535596140526E-5	0.0	0.0	28.139703186025802	0.0
28	4.850535596140526E-5	0.0	0.0	28.70784642040174	0.0
29	4.850535596140526E-5	0.0	0.0	29.286224284885538	0.0
30	4.850535596140526E-5	0.0	0.0	29.817260921951	0.0
31	4.850535596140526E-5	0.0	0.0	30.31002683316292	0.0
32	4.850535596140526E-5	0.0	0.0	30.87690892828386	0.0
33	4.850535596140526E-5	0.0	0.0	31.412505068809697	0.0
34	4.850535596140526E-5	0.0	0.0	32.025467252093975	0.0
35	4.850535596140526E-5	0.0	0.0	32.50848358675765	0.0
36	4.850535596140526E-5	0.0	0.0	33.02365897242373	0.0
37	4.850535596140526E-5	0.0	0.0	33.58481743554123	0.0
38	4.850535596140526E-5	0.0	0.0	34.22135322182275	0.0
39	4.850535596140526E-5	0.0	0.0	34.86652296146541	0.0
40	9.701071192281052E-5	0.0	0.0	35.475798737696614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26445	0.0	45.425976	1
TACGGGT	225	0.0	44.97778	3
TCACGAC	490	0.0	44.122448	24
CGACGGT	460	0.0	43.5	27
CTAGCGG	365	0.0	42.219177	1
CGGGTAC	535	0.0	42.13084	5
TACGCGG	175	0.0	42.057144	1
CGTTCAG	1460	0.0	41.746574	38
ACGGGTA	215	0.0	41.720932	4
ACCCGCA	4460	0.0	41.56502	31
TACGGGA	515	0.0	41.53398	3
CCCGCAC	4460	0.0	41.513454	32
GCACTTA	4430	0.0	41.48307	35
TCGGCGT	1660	0.0	41.42771	4
CGCACTT	4450	0.0	41.4	34
GTCGGCG	1650	0.0	41.4	3
ATAACGG	100	0.0	41.399998	1
GTTTTTT	29155	0.0	41.353455	2
CCGCACT	4440	0.0	41.337837	33
GTTGATC	1845	0.0	41.262875	15
>>END_MODULE