SRR1043281_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043281_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2009714
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96521	4.802723173546087	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	15843	0.7883211242992784	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9625	0.47892386677905413	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	8372	0.41657668703109	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	7153	0.3559212902930467	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	5854	0.2912852276493073	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	5123	0.2549118929360098	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4380	0.21794145833685788	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3985	0.1982869204274837	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3978	0.19793861216073533	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	3769	0.18753912248210441	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	2832	0.14091557306163963	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2821	0.1403682314996064	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2711	0.13489481587927438	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	2608	0.12976970852569072	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	2459	0.12235571827633185	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2306	0.11474269473168819	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2275	0.11320018669323097	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAC	20	7.034548E-4	45.000004	10
CGTTTTT	24100	0.0	44.645226	1
CTAGCGG	530	0.0	43.301888	1
ACGGGTA	250	0.0	42.3	4
CACGACG	500	0.0	41.399998	25
CGGTCTA	495	0.0	41.363636	30
CACGTTC	60	3.6379788E-12	41.250004	45
ACATACG	240	0.0	41.250004	16
GACGGTC	500	0.0	40.949997	28
TCACGAC	510	0.0	40.588234	24
CGACGGT	510	0.0	40.588234	27
TCGGCGT	1440	0.0	40.468754	4
GTTTTTT	26805	0.0	40.43369	2
ACCCGCA	4130	0.0	40.26029	31
CTTACTG	4060	0.0	40.17857	38
CGCACTT	4090	0.0	40.103912	34
GCACTTA	4085	0.0	40.09792	35
CCCGCAC	4190	0.0	40.005966	32
GCATAGG	405	0.0	40.0	1
CCGCACT	4135	0.0	39.885128	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content