##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043281_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2009714
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31767455468788	33.0	31.0	34.0	31.0	34.0
2	32.60678036775382	34.0	31.0	34.0	31.0	34.0
3	32.70382502186878	34.0	31.0	34.0	31.0	34.0
4	36.184822318001466	37.0	37.0	37.0	35.0	37.0
5	36.176897807349704	37.0	37.0	37.0	35.0	37.0
6	36.3366707899731	37.0	37.0	37.0	35.0	37.0
7	36.293061599809725	37.0	37.0	37.0	35.0	37.0
8	36.21392546402125	37.0	37.0	37.0	35.0	37.0
9	37.76841530685461	39.0	38.0	39.0	35.0	39.0
10	37.632107354578814	39.0	37.0	39.0	35.0	39.0
11	37.40761123224499	39.0	37.0	39.0	35.0	39.0
12	37.40812424056358	39.0	37.0	39.0	35.0	39.0
13	37.400561472926	39.0	37.0	39.0	35.0	39.0
14	38.81568521690151	41.0	38.0	41.0	35.0	41.0
15	38.8753529109117	41.0	38.0	41.0	35.0	41.0
16	38.812930596094766	41.0	38.0	41.0	35.0	41.0
17	38.790077593130164	41.0	38.0	41.0	35.0	41.0
18	38.580863247208306	40.0	38.0	41.0	35.0	41.0
19	38.333201639636286	40.0	37.0	41.0	35.0	41.0
20	38.08856583573583	40.0	35.0	41.0	34.0	41.0
21	38.02349189984246	40.0	35.0	41.0	34.0	41.0
22	37.98396239464919	40.0	35.0	41.0	34.0	41.0
23	37.95815026416694	40.0	35.0	41.0	34.0	41.0
24	37.857639445214595	40.0	35.0	41.0	34.0	41.0
25	37.83174770141423	40.0	35.0	41.0	34.0	41.0
26	37.78715528677215	40.0	35.0	41.0	34.0	41.0
27	37.75958569229253	40.0	35.0	41.0	34.0	41.0
28	37.650684624777455	40.0	35.0	41.0	34.0	41.0
29	37.628663580987144	40.0	35.0	41.0	34.0	41.0
30	37.44735619098041	39.0	35.0	41.0	34.0	41.0
31	37.34124855576465	40.0	35.0	41.0	33.0	41.0
32	37.0716594500511	39.0	35.0	41.0	33.0	41.0
33	36.685999599943074	39.0	35.0	41.0	31.0	41.0
34	36.390175915578034	39.0	35.0	41.0	31.0	41.0
35	36.193477778430164	39.0	35.0	41.0	30.0	41.0
36	36.11993796132186	39.0	35.0	41.0	30.0	41.0
37	36.02663662590796	39.0	35.0	41.0	30.0	41.0
38	35.95824878564811	39.0	35.0	41.0	29.0	41.0
39	35.884734345284954	39.0	35.0	41.0	29.0	41.0
40	35.7804901592963	39.0	35.0	41.0	29.0	41.0
41	35.722505789380975	39.0	35.0	41.0	29.0	41.0
42	35.619955874318435	38.0	35.0	41.0	28.0	41.0
43	35.48998962041365	38.0	35.0	41.0	27.0	41.0
44	35.3431881352272	38.0	35.0	40.0	26.0	41.0
45	35.27362599852516	38.0	35.0	40.0	26.0	41.0
46	35.18553087653268	38.0	35.0	40.0	26.0	41.0
47	35.03348138093281	37.0	35.0	40.0	25.0	41.0
48	34.975844821700996	37.0	35.0	40.0	25.0	41.0
49	34.90739030528722	37.0	35.0	40.0	26.0	41.0
50	34.83515166834684	37.0	35.0	40.0	25.0	41.0
51	34.41164613472365	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	2.0
14	12.0
15	20.0
16	71.0
17	139.0
18	351.0
19	726.0
20	1284.0
21	2062.0
22	3530.0
23	5412.0
24	9601.0
25	18057.0
26	29186.0
27	36297.0
28	36443.0
29	34409.0
30	34183.0
31	37777.0
32	44100.0
33	56326.0
34	116510.0
35	179080.0
36	172163.0
37	168031.0
38	292026.0
39	731682.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.5717271213715	17.433774158910172	23.607538187025618	30.386960532692715
2	35.32895725461434	19.334392853908568	23.99202075519203	21.34462913628506
3	25.201993915552162	20.380959678839876	34.813261986531415	19.603784419076543
4	22.291380763631043	23.36566297493076	33.31852193894256	21.02443432249564
5	20.16301822050302	31.389192691099332	30.082389832583146	18.36539925581451
6	76.93308600129173	1.71273126424954	18.37161904629216	2.982563688166575
7	77.2214852461594	1.5566891607462554	16.412783112422964	4.809042480671379
8	68.55184369517254	2.9614164005425647	21.683831629774186	6.802908274510701
9	34.7287225943592	18.07729856088976	26.75410531050687	20.439873534244178
10	27.515507181618876	19.221242425539156	36.23734521429417	17.02590517854779
11	26.088985795988883	15.331783527407383	37.44612417488259	21.13310650172114
12	21.920084151277248	17.072578486292077	39.75361668376694	21.25372067866373
13	17.332764761553136	20.806741655777884	39.63683389775859	22.22365968491039
14	16.735764392346375	21.074939021174156	40.28359259078655	21.905703995692917
15	19.252689686194156	16.381485126739427	42.17530454582095	22.19052064124547
16	18.21249192671196	21.095986792150526	37.98983337927685	22.701687901860662
17	18.871739959019042	21.700052843339897	37.69302497768339	21.735182219957665
18	20.12624681919915	27.003543787822547	34.818387093885	18.051822299093303
19	19.97119988217229	22.3501951023877	34.284579795931165	23.394025219508848
20	19.283141780372727	26.345042130372782	35.43275311810537	18.939062971149127
21	16.961269115904052	20.7319051367508	36.40234381608528	25.90448193125987
22	17.620019564972928	24.101190517655745	33.991851576891044	24.286938340480287
23	18.35932874030832	22.578735083698476	38.37904298820628	20.68289318778692
24	23.12139936329249	20.275223240719825	34.404397839692614	22.198979556295075
25	17.972358255950844	21.983774805768384	35.9085919688075	24.13527496947327
26	17.47258565149071	20.457935805791273	36.394382484273876	25.675096058444137
27	21.349903518610112	21.6129757766528	36.8246427103558	20.21247799438129
28	20.11002560563344	22.809812739524133	33.31837266397109	23.761788990871338
29	22.690940103915285	21.05214970886405	34.748178098973284	21.50873208824738
30	22.867333361861437	21.762051714821116	34.07300740304342	21.297607520274028
31	23.780348845656647	20.67707146389984	31.163986517484577	24.378593172958936
32	21.770908696461287	21.86470313686425	31.960617281861996	24.403770884812463
33	18.51168872784884	22.818570204516664	33.787892207547934	24.88184886008656
34	18.431577826496707	21.93003581604149	34.91909794129911	24.719288416162698
35	19.29682531942356	26.169544522255407	30.463538593053542	24.070091565267496
36	18.264887441695684	25.435360454273592	31.14960636190025	25.15014574213047
37	18.667083973142446	25.933789583990556	33.05704194726215	22.342084495604848
38	16.69705241641348	22.4081635496394	30.732780883250054	30.162003150697064
39	20.741558251572116	19.896015054878454	34.45117066408454	24.91125602946489
40	17.30922907438571	18.983497154321462	37.11199703042323	26.595276740869593
41	19.26652250021645	23.592162864964866	31.402279130264304	25.73903550455438
42	18.289418295339534	18.92552870706976	34.28184308812099	28.503209909469707
43	21.59969030419254	21.5310735756431	33.005890390373956	23.86334572979041
44	20.16401338697944	22.444686159324164	34.11087348747135	23.280426966225047
45	21.25377043698755	21.316167375059337	33.05281248973734	24.377249698215767
46	21.658056818034805	20.63885707120516	32.744410398693546	24.958675712066494
47	18.048189941454357	22.623965400051947	34.131771983476256	25.19607267501744
48	19.452369839688632	20.63985223768158	31.300224808107025	28.60755311452276
49	19.585871422500915	17.993455785251037	36.05214473303167	26.368528059216384
50	18.730824385957405	18.617474924292708	36.99262681157617	25.65907387817371
51	17.627931138460497	17.30962714097628	35.95626044302821	29.106181277535015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	385.0
1	2192.5
2	4000.0
3	62717.5
4	121435.0
5	69897.5
6	18360.0
7	16751.5
8	15143.0
9	14892.5
10	14642.0
11	14374.0
12	14106.0
13	13470.5
14	12835.0
15	11924.5
16	11014.0
17	10262.0
18	9510.0
19	8782.5
20	8055.0
21	7529.0
22	7003.0
23	6912.5
24	6822.0
25	6638.5
26	7535.0
27	8615.0
28	9291.5
29	9968.0
30	11695.5
31	13423.0
32	15345.5
33	17268.0
34	20181.0
35	23094.0
36	26111.0
37	29128.0
38	32550.0
39	35972.0
40	41239.0
41	46506.0
42	56983.0
43	67460.0
44	80269.0
45	93078.0
46	123917.0
47	154756.0
48	197107.0
49	239458.0
50	247049.5
51	254641.0
52	214666.5
53	174692.0
54	151352.5
55	128013.0
56	114155.0
57	100297.0
58	91893.5
59	83490.0
60	77260.0
61	71030.0
62	64785.0
63	58540.0
64	56201.5
65	53863.0
66	41746.0
67	29629.0
68	24271.5
69	18914.0
70	16668.0
71	14422.0
72	14513.0
73	14604.0
74	12478.5
75	7594.5
76	4836.0
77	3469.0
78	2102.0
79	1537.0
80	972.0
81	694.0
82	416.0
83	324.5
84	233.0
85	187.5
86	142.0
87	80.0
88	18.0
89	11.5
90	5.0
91	5.5
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2009714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.666180383226717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93289265982939	22.979056029432936
2	10.565924491873439	6.480330927666879
3	3.776494820826206	3.474320141753337
4	1.8771048123316147	2.3025453909273694
5	1.1529320857667655	1.7678011655866723
6	0.8069001527500018	1.4846727381290847
7	0.6262885677629916	1.3444114733680825
8	0.48668997122973073	1.1939937958750675
9	0.4190843881841504	1.1566545699464426
>10	4.873245848801881	32.080859867247305
>50	0.33750689039546333	6.8331281690312355
>100	0.1328302060008525	7.259697092875686
>500	0.006052552123812285	1.2070080683655942
>1k	0.004907474694982934	2.9865435912948612
>5k	8.179124491638224E-4	1.8122929423483571
>10k+	3.2716497966552894E-4	5.636684036151101
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96521	4.802723173546087	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	15843	0.7883211242992784	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9625	0.47892386677905413	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	8372	0.41657668703109	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	7153	0.3559212902930467	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	5854	0.2912852276493073	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	5123	0.2549118929360098	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4380	0.21794145833685788	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3985	0.1982869204274837	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3978	0.19793861216073533	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	3769	0.18753912248210441	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	2832	0.14091557306163963	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2821	0.1403682314996064	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2711	0.13489481587927438	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	2608	0.12976970852569072	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	2459	0.12235571827633185	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2306	0.11474269473168819	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2275	0.11320018669323097	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3695053126962344	0.0
2	0.0	0.0	0.0	1.2430624457012291	0.0
3	0.0	0.0	0.0	2.1348808835486044	0.0
4	0.0	0.0	0.0	2.6711760976935026	0.0
5	0.0	0.0	0.0	5.45609972364227	0.0
6	0.0	0.0	0.0	6.415489965238835	0.0
7	0.0	0.0	0.0	9.063279650736373	0.0
8	0.0	0.0	0.0	11.797748336330443	0.0
9	0.0	0.0	0.0	14.578840571344978	0.0
10	0.0	0.0	0.0	16.160458652325655	0.0
11	0.0	0.0	0.0	17.40585973924648	0.0
12	0.0	0.0	0.0	18.340022510665698	0.0
13	0.0	0.0	0.0	18.807900029556443	0.0
14	0.0	0.0	0.0	19.136354725100187	0.0
15	0.0	0.0	0.0	20.02518766351829	0.0
16	0.0	0.0	0.0	20.97273542404541	0.0
17	0.0	0.0	0.0	22.121854154372215	0.0
18	0.0	0.0	0.0	22.73189120442013	0.0
19	0.0	0.0	0.0	23.42129278096286	0.0
20	0.0	0.0	0.0	24.19712456598302	0.0
21	0.0	0.0	0.0	24.9622085530578	0.0
22	0.0	0.0	0.0	25.744707953469995	0.0
23	4.975832382120043E-5	0.0	0.0	26.40510042722497	0.0
24	4.975832382120043E-5	0.0	0.0	26.981152542103004	0.0
25	4.975832382120043E-5	0.0	0.0	27.52516029644019	0.0
26	4.975832382120043E-5	0.0	0.0	28.087976697181787	0.0
27	4.975832382120043E-5	0.0	0.0	28.680100750654073	0.0
28	4.975832382120043E-5	0.0	0.0	29.252520507893163	0.0
29	4.975832382120043E-5	0.0	0.0	29.831607880524295	0.0
30	9.951664764240086E-5	0.0	0.0	30.366907928192767	0.0
31	1.9903329528480173E-4	0.0	0.0	30.867924490748436	0.0
32	1.9903329528480173E-4	0.0	0.0	31.431537024671172	0.0
33	1.9903329528480173E-4	0.0	0.0	31.974201304265183	0.0
34	1.9903329528480173E-4	0.0	0.0	32.59513542722994	0.0
35	1.9903329528480173E-4	0.0	0.0	33.07843802650526	0.0
36	1.9903329528480173E-4	0.0	0.0	33.59194392834005	0.0
37	1.9903329528480173E-4	0.0	0.0	34.156203320472464	0.0
38	1.9903329528480173E-4	0.0	0.0	34.803608871710104	0.0
39	1.9903329528480173E-4	0.0	0.0	35.46564337015118	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAC	20	7.034548E-4	45.000004	10
CGTTTTT	24100	0.0	44.645226	1
CTAGCGG	530	0.0	43.301888	1
ACGGGTA	250	0.0	42.3	4
CACGACG	500	0.0	41.399998	25
CGGTCTA	495	0.0	41.363636	30
CACGTTC	60	3.6379788E-12	41.250004	45
ACATACG	240	0.0	41.250004	16
GACGGTC	500	0.0	40.949997	28
TCACGAC	510	0.0	40.588234	24
CGACGGT	510	0.0	40.588234	27
TCGGCGT	1440	0.0	40.468754	4
GTTTTTT	26805	0.0	40.43369	2
ACCCGCA	4130	0.0	40.26029	31
CTTACTG	4060	0.0	40.17857	38
CGCACTT	4090	0.0	40.103912	34
GCACTTA	4085	0.0	40.09792	35
CCCGCAC	4190	0.0	40.005966	32
GCATAGG	405	0.0	40.0	1
CCGCACT	4135	0.0	39.885128	33
>>END_MODULE