FastQCFastQC Report
Thu 26 May 2016
SRR1043280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043280_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2486286
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1254445.0454372505817915No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC141270.568196900919685No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA96590.3884911068155473No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT94710.3809296275649704TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT77350.3111066064000682TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC59780.24043895191462283TruSeq Adapter, Index 22 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT51960.20898641588296762No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC45410.18264190040888298No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT44690.17974601473844923TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG39440.15863018172486995No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT36360.14624222635690343No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34260.13779589315147173No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33640.13530221382415378No Hit
GGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT30570.12295447909049884TruSeq Adapter, Index 23 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG30150.12126521244941249No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT26400.10618247458257014No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA25510.10260283812883957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA206.312878E-446.03
CGTTTTT328250.045.3483621
TATACGG1750.043.371431
TCGGCGT13950.042.207894
GGCGATT4600.042.07
AGGGCGA10750.041.9348835
ACGGGAT4900.041.7755134
CACGACG5250.041.61904525
TAGTAGG5050.041.445541
CACTTAC37650.041.41832436
TACGGGA4450.041.3483123
CGGTCTA5350.041.27102730
GTTTTTT368200.040.940252
ACGGTCT5350.040.8411229
TCACGAC5500.040.56363724
CTCACGA5500.040.56363723
GCACTTA38450.040.55656435
CGACGGT5400.040.46296327
AGCACGG3700.040.4054071
CCTACGG1200.040.251
Produced by FastQC (version 0.11.5)