##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043280_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2486286
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33897266847016	33.0	31.0	34.0	31.0	34.0
2	32.673417297929525	34.0	31.0	34.0	31.0	34.0
3	32.77735103684773	34.0	31.0	34.0	31.0	34.0
4	36.217190218663504	37.0	37.0	37.0	35.0	37.0
5	36.154443213693035	37.0	37.0	37.0	35.0	37.0
6	36.31910367512024	37.0	37.0	37.0	35.0	37.0
7	36.25431587516481	37.0	37.0	37.0	35.0	37.0
8	36.23665217919419	37.0	37.0	37.0	35.0	37.0
9	37.82425392734384	39.0	38.0	39.0	35.0	39.0
10	37.65056916219614	39.0	37.0	39.0	35.0	39.0
11	37.34028667659312	39.0	37.0	39.0	35.0	39.0
12	37.3449060164438	39.0	37.0	39.0	35.0	39.0
13	37.296175902530926	39.0	37.0	39.0	34.0	39.0
14	38.64220769452911	40.0	38.0	41.0	35.0	41.0
15	38.709908675027734	41.0	38.0	41.0	35.0	41.0
16	38.60739472450072	41.0	38.0	41.0	34.0	41.0
17	38.57870011736381	40.0	38.0	41.0	34.0	41.0
18	38.33938332114648	40.0	38.0	41.0	34.0	41.0
19	38.082024754996006	40.0	37.0	41.0	34.0	41.0
20	37.75823738701018	40.0	35.0	41.0	34.0	41.0
21	37.69455243684757	39.0	35.0	41.0	34.0	41.0
22	37.64720631496135	39.0	35.0	41.0	34.0	41.0
23	37.655996534590145	39.0	35.0	41.0	34.0	41.0
24	37.57137352661761	39.0	35.0	41.0	34.0	41.0
25	37.47093214537668	39.0	35.0	41.0	34.0	41.0
26	37.38304161307267	39.0	35.0	41.0	33.0	41.0
27	37.399149172701776	39.0	35.0	41.0	34.0	41.0
28	37.326011569063255	39.0	35.0	41.0	33.0	41.0
29	37.23072245107763	39.0	35.0	41.0	33.0	41.0
30	37.16174285661425	39.0	35.0	41.0	33.0	41.0
31	36.95790870398659	39.0	35.0	41.0	33.0	41.0
32	36.70561431790229	39.0	35.0	41.0	32.0	41.0
33	36.262521286770706	39.0	35.0	41.0	30.0	41.0
34	35.99171052726838	39.0	35.0	41.0	30.0	41.0
35	35.79432856879699	39.0	35.0	41.0	29.0	41.0
36	35.797740485205644	39.0	35.0	41.0	29.0	41.0
37	35.7587795611607	39.0	35.0	41.0	29.0	41.0
38	35.688445335733704	38.0	35.0	41.0	29.0	41.0
39	35.678562723677004	38.0	35.0	41.0	29.0	41.0
40	35.628033138585025	38.0	35.0	41.0	29.0	41.0
41	35.57093793714802	38.0	35.0	41.0	29.0	41.0
42	35.47024035046652	38.0	35.0	41.0	28.0	41.0
43	35.3467819068281	38.0	35.0	41.0	27.0	41.0
44	35.16360265874481	37.0	35.0	40.0	26.0	41.0
45	35.06053125022624	37.0	35.0	40.0	25.0	41.0
46	35.02259072367379	37.0	35.0	40.0	25.0	41.0
47	34.93367738063923	37.0	35.0	40.0	25.0	41.0
48	34.83696203896093	37.0	35.0	40.0	25.0	41.0
49	34.786189119031356	36.0	35.0	40.0	26.0	41.0
50	34.68762765023815	36.0	35.0	40.0	25.0	41.0
51	34.572187994462425	36.0	35.0	40.0	24.0	41.0
52	34.20100020673406	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	2.0
13	8.0
14	23.0
15	54.0
16	85.0
17	250.0
18	507.0
19	1045.0
20	1937.0
21	3192.0
22	5124.0
23	8089.0
24	13813.0
25	24847.0
26	37932.0
27	45217.0
28	46857.0
29	45732.0
30	46571.0
31	51820.0
32	60274.0
33	75881.0
34	167467.0
35	226579.0
36	233319.0
37	225160.0
38	343378.0
39	819601.0
40	1518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.001283842647222	16.70009001377959	23.210885634235158	32.087740509338026
2	36.80099554114048	18.773624595078765	22.63110519063374	21.794274673147015
3	25.159977572974306	20.09245919415546	34.877765470263675	19.869797762606556
4	22.525083598588417	22.420349066841062	33.531419957317866	21.52314737725266
5	21.114787277087192	30.446617967522645	30.2589082671905	18.179686488199668
6	75.92002689956023	1.9593079798542887	19.08831083793256	3.032354282652921
7	75.38473047750742	1.7923923474612333	17.827957041144906	4.994920133886447
8	66.16853411071776	3.4825840631367426	23.140620186092832	7.2082616400526724
9	38.960924044940924	15.96244358050522	27.52857072758323	17.548061646970623
10	26.838666187236704	15.067856232147067	37.964578491774475	20.128899088841752
11	24.436569244246236	12.465661633456488	41.36587665296752	21.731892469329754
12	20.909983807172626	13.52366541902259	43.52226574094855	22.044085032856238
13	16.736610349734505	16.61964874515643	45.01384796439348	21.62989294071559
14	16.634007511605663	17.05226188781178	44.64486386521905	21.66886673536351
15	20.10520913523223	14.656801349482723	43.97056493098541	21.267424584299636
16	19.17659513024648	18.80033109626165	40.470927318900564	21.552146454591306
17	19.822216752215958	19.7143450109923	40.000748103798195	20.46269013299355
18	20.095837727437633	25.334132919543446	36.40844215025946	18.161587202759456
19	21.20777738361556	21.391505241150856	35.16292976753278	22.237787607700803
20	20.57140650753775	25.18889620904433	35.60772976238454	18.63196752103338
21	18.3503426395837	20.229048468277583	36.86092428626473	24.559684605873983
22	19.602410985703173	22.019027577680124	34.78598198276465	23.59257945385205
23	20.135776817309033	19.740206878854647	37.6576146107085	22.46640169312782
24	22.943337974794535	18.370131191665	34.52519138988837	24.161339443652096
25	17.9964010576418	19.264356554314347	37.44271576158174	25.296526626462118
26	17.787897289370573	18.163477572572102	37.22713316167167	26.82149197638566
27	21.358846086089855	19.781674352829885	36.76853748925104	22.090942071829225
28	22.570532915360502	20.653939249145107	33.603455113369904	23.172072722124486
29	21.51787847415784	18.08279498014307	36.009735002328775	24.38959154337031
30	25.878237660510493	17.47011405767478	32.873732145054916	23.77791613675981
31	24.603846862348096	17.345550753211818	33.43859073332674	24.612011651113345
32	24.70307116719476	17.15538759418667	32.77933431632564	25.36220692229293
33	20.634311579601057	17.9106104446552	36.11169431030863	25.34338366543511
34	20.80874847061038	19.234472623020842	37.62467391120732	22.33210499516146
35	20.470492936049997	22.898612629440056	34.89691049219599	21.73398394231396
36	20.92892772593338	20.782403955136296	33.69278514217592	24.595883176754405
37	19.608041874506796	24.576979478627962	34.10625326289896	21.708725383966286
38	18.984340498237128	21.758679411781266	32.62609369959852	26.630886390383086
39	20.500135543537628	20.176681202403906	34.71507300447334	24.608110249585124
40	18.084645129321405	19.297216812546907	35.78309172798303	26.835046330148664
41	19.556760565759532	22.336046617323994	31.73062149728551	26.37657131963097
42	19.547228275427685	17.917608835025415	34.398094185463776	28.137068704083116
43	21.936374174169828	18.771372239557316	35.21030967475182	24.08194391152104
44	21.304668891672158	19.1801345460659	35.41507292403207	24.100123638229874
45	21.64726825473819	20.276146831056444	34.08421235529621	23.992372558909153
46	21.962799130912533	19.039281884706746	34.029351410095224	24.9685675742855
47	19.754605865938192	20.11208686369951	35.23778036798662	24.89552690237567
48	20.927640665635412	18.2339843445203	32.59608910640208	28.242285883442207
49	20.066114678681373	16.589241945616877	37.043204200964816	26.301439174736934
50	19.593079798542888	16.8415057640191	37.95150678562321	25.61390765181479
51	18.236679127019176	15.72795728246871	37.30479920652733	28.730564383984785
52	16.16712638851685	16.628497284704977	40.56882434281495	26.63555198396323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	456.0
1	3032.5
2	5609.0
3	87271.0
4	168933.0
5	96544.5
6	24156.0
7	21853.0
8	19550.0
9	19152.5
10	18755.0
11	18104.0
12	17453.0
13	16474.0
14	14347.5
15	13200.0
16	12471.0
17	11742.0
18	10923.0
19	10104.0
20	9813.0
21	9522.0
22	9210.0
23	8898.0
24	8957.0
25	9016.0
26	10103.5
27	11191.0
28	11772.5
29	12354.0
30	13057.5
31	13761.0
32	15757.0
33	17753.0
34	20885.5
35	24018.0
36	26222.0
37	28426.0
38	31250.0
39	36703.5
40	39333.0
41	46082.5
42	52832.0
43	66670.5
44	80509.0
45	103926.0
46	127343.0
47	166378.0
48	205413.0
49	232146.5
50	258880.0
51	250992.5
52	243105.0
53	213436.5
54	183768.0
55	166415.0
56	149062.0
57	136957.0
58	124852.0
59	120233.0
60	115614.0
61	112204.0
62	108794.0
63	106630.0
64	88038.5
65	71611.0
66	59542.0
67	47473.0
68	39099.0
69	30725.0
70	26035.5
71	21346.0
72	21445.5
73	21545.0
74	16842.5
75	12140.0
76	9165.5
77	6191.0
78	4790.5
79	3390.0
80	2602.0
81	1814.0
82	1324.5
83	835.0
84	628.5
85	422.0
86	310.5
87	199.0
88	130.0
89	66.5
90	72.0
91	40.0
92	8.0
93	12.0
94	16.0
95	8.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2486286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.97717665512254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8408816091171	23.183592106307323
2	9.677072037518867	5.9953674002113715
3	3.595375585295324	3.3412375394162357
4	1.9239442525268717	2.3839344394053037
5	1.250498272433032	1.9368452946041794
6	0.9004226693137777	1.6735531254965927
7	0.7661557576189514	1.661333957437105
8	0.6454103743123524	1.5994392944098001
9	0.5720903820332063	1.5949570344245256
>10	5.4364920868782765	32.38002214547933
>50	0.23538810082945247	4.979194214335134
>100	0.14480134973250428	7.976705935962748
>500	0.00586407394961802	1.203750266187223
>1k	0.004691259159694416	2.9204466971108993
>5k	6.5156377217978E-4	1.5355283828134063
>10k+	2.60625508871912E-4	5.634092166398958
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125444	5.0454372505817915	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14127	0.568196900919685	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9659	0.3884911068155473	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	9471	0.3809296275649704	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT	7735	0.3111066064000682	TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	5978	0.24043895191462283	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	5196	0.20898641588296762	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	4541	0.18264190040888298	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	4469	0.17974601473844923	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	3944	0.15863018172486995	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	3636	0.14624222635690343	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3426	0.13779589315147173	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3364	0.13530221382415378	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	3057	0.12295447909049884	TruSeq Adapter, Index 23 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	3015	0.12126521244941249	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	2640	0.10618247458257014	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2551	0.10260283812883957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3211617649779631	0.0
2	0.0	0.0	0.0	1.036606408112341	0.0
3	0.0	0.0	0.0	1.7530565671045084	0.0
4	0.0	0.0	0.0	2.262652003832222	0.0
5	0.0	0.0	0.0	4.659721367533743	0.0
6	0.0	0.0	0.0	5.744471874917045	0.0
7	0.0	0.0	0.0	8.522390424914914	0.0
8	0.0	0.0	0.0	11.6195803700781	0.0
9	0.0	0.0	0.0	14.685599323649813	0.0
10	0.0	0.0	0.0	16.368792648955107	0.0
11	0.0	0.0	0.0	17.692493944783504	0.0
12	0.0	0.0	0.0	18.592068651796293	0.0
13	0.0	0.0	0.0	19.074877146072495	0.0
14	0.0	0.0	0.0	19.380513746206187	0.0
15	0.0	0.0	0.0	20.070699830992893	0.0
16	4.02206343115796E-5	0.0	0.0	20.815746860980596	0.0
17	4.02206343115796E-5	0.0	0.0	21.750957049993445	0.0
18	4.02206343115796E-5	0.0	0.0	22.29293009734198	0.0
19	8.04412686231592E-5	0.0	0.0	22.90939175943556	0.0
20	8.04412686231592E-5	0.0	0.0	23.56816552882492	0.0
21	2.0110317155789802E-4	0.0	0.0	24.28690826397285	0.0
22	2.4132380586947761E-4	0.0	0.0	24.936431287470548	0.0
23	2.4132380586947761E-4	0.0	0.0	25.51778033581012	0.0
24	3.217650744926368E-4	0.0	0.0	25.990694554045675	0.0
25	3.217650744926368E-4	0.0	0.0	26.46469472940764	0.0
26	3.217650744926368E-4	0.0	0.0	26.949393593496485	0.0
27	3.217650744926368E-4	0.0	0.0	27.473267355404808	0.0
28	3.217650744926368E-4	0.0	0.0	27.97646771127698	0.0
29	3.217650744926368E-4	0.0	0.0	28.47830056558256	0.0
30	3.217650744926368E-4	0.0	0.0	28.937016899906126	0.0
31	3.217650744926368E-4	0.0	0.0	29.39625610247574	0.0
32	3.217650744926368E-4	0.0	0.0	29.906816834426934	0.0
33	3.6198570880421644E-4	0.0	0.0	30.392521214373566	0.0
34	3.6198570880421644E-4	0.0	0.0	30.92166387937671	0.0
35	4.0220634311579603E-4	0.0	0.0	31.409178187867365	0.0
36	4.0220634311579603E-4	0.0	0.0	31.912820970716965	0.0
37	4.4242697742737563E-4	0.0	0.0	32.54102705802953	0.0
38	4.4242697742737563E-4	0.0	0.0	33.20700032096066	0.0
39	4.4242697742737563E-4	0.0	0.0	33.893285004219145	0.0
40	4.4242697742737563E-4	0.0	0.0	34.510349975827395	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTA	20	6.312878E-4	46.0	3
CGTTTTT	32825	0.0	45.348362	1
TATACGG	175	0.0	43.37143	1
TCGGCGT	1395	0.0	42.20789	4
GGCGATT	460	0.0	42.0	7
AGGGCGA	1075	0.0	41.934883	5
ACGGGAT	490	0.0	41.775513	4
CACGACG	525	0.0	41.619045	25
TAGTAGG	505	0.0	41.44554	1
CACTTAC	3765	0.0	41.418324	36
TACGGGA	445	0.0	41.348312	3
CGGTCTA	535	0.0	41.271027	30
GTTTTTT	36820	0.0	40.94025	2
ACGGTCT	535	0.0	40.84112	29
TCACGAC	550	0.0	40.563637	24
CTCACGA	550	0.0	40.563637	23
GCACTTA	3845	0.0	40.556564	35
CGACGGT	540	0.0	40.462963	27
AGCACGG	370	0.0	40.405407	1
CCTACGG	120	0.0	40.25	1
>>END_MODULE