FastQCFastQC Report
Thu 26 May 2016
SRR1043279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043279_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2438543
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1204044.937538521978083No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC133660.5481141812959625No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC92770.38043208588079025TruSeq Adapter, Index 23 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG82770.3394239921133234No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT74650.3061254199741403TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG57230.23468932063121298No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC50320.20635272783789335No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC44560.18273206582783244TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT43860.17986149926410977No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC36770.15078676078297573No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT33450.13717207365217673No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33020.13540872562017567No Hit
GGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC32690.13405545852584924No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC32600.13368638568194205No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT29410.1206048037701201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT317600.044.3694881
CGGGTAT1450.043.4482735
CACGACG3800.042.63157725
CGGGATA2350.042.127665
ACGGGTA1700.041.029414
CACTTAC34650.040.97402636
TCGGCGT14400.040.4687544
GTTTTTT356150.040.2239232
CGCACTT35350.040.0990134
CGACGGT4000.039.937527
ACCCGCA36000.039.937531
CCCGCAC36250.039.91034732
CATGCGG3500.039.8571431
ACTTACT35350.039.84441437
CCGCACT35550.039.74683433
TACGCGG3800.039.671051
CTTACTG35400.039.66101538
GCACTTA35750.039.65035235
TCACGAC4150.039.57831624
ACGGGAT4950.039.5454524