##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043279_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2438543
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30604381386755	33.0	31.0	34.0	31.0	34.0
2	32.613349036699375	34.0	31.0	34.0	31.0	34.0
3	32.69755874716993	34.0	31.0	34.0	31.0	34.0
4	36.18887671859795	37.0	37.0	37.0	35.0	37.0
5	36.1752919673756	37.0	37.0	37.0	35.0	37.0
6	36.338709221038954	37.0	37.0	37.0	35.0	37.0
7	36.28594082614086	37.0	37.0	37.0	35.0	37.0
8	36.19147253093343	37.0	37.0	37.0	35.0	37.0
9	37.78859384476714	39.0	38.0	39.0	35.0	39.0
10	37.600254332197544	39.0	37.0	39.0	35.0	39.0
11	37.338769092855856	39.0	37.0	39.0	35.0	39.0
12	37.3472270121954	39.0	37.0	39.0	35.0	39.0
13	37.313410097750996	39.0	37.0	39.0	35.0	39.0
14	38.65681761609289	40.0	38.0	41.0	35.0	41.0
15	38.73328499846015	41.0	38.0	41.0	35.0	41.0
16	38.66546335250188	41.0	38.0	41.0	34.0	41.0
17	38.615004943525705	40.0	38.0	41.0	34.0	41.0
18	38.39528644768618	40.0	38.0	41.0	34.0	41.0
19	38.157653566084335	40.0	37.0	41.0	34.0	41.0
20	37.85981137097029	40.0	35.0	41.0	34.0	41.0
21	37.80079375266296	40.0	35.0	41.0	34.0	41.0
22	37.77028701154747	40.0	35.0	41.0	34.0	41.0
23	37.73961951870441	40.0	35.0	41.0	34.0	41.0
24	37.66486627465663	39.0	35.0	41.0	34.0	41.0
25	37.62799876811686	39.0	35.0	41.0	34.0	41.0
26	37.595432190451426	39.0	35.0	41.0	34.0	41.0
27	37.56066429831256	39.0	35.0	41.0	34.0	41.0
28	37.45967366579142	39.0	35.0	41.0	34.0	41.0
29	37.385286624020985	39.0	35.0	41.0	34.0	41.0
30	37.264450124521076	39.0	35.0	41.0	33.0	41.0
31	37.047378291053306	39.0	35.0	41.0	33.0	41.0
32	36.7994966666571	39.0	35.0	41.0	33.0	41.0
33	36.34837072793057	39.0	35.0	41.0	31.0	41.0
34	36.045843768184525	39.0	35.0	41.0	30.0	41.0
35	35.846774077799736	39.0	35.0	41.0	30.0	41.0
36	35.82189856812039	39.0	35.0	41.0	29.0	41.0
37	35.73475595878359	38.0	35.0	41.0	29.0	41.0
38	35.659761177063515	38.0	35.0	41.0	29.0	41.0
39	35.60237322040251	38.0	35.0	41.0	28.0	41.0
40	35.52452673584185	38.0	35.0	41.0	28.0	41.0
41	35.43303029718976	38.0	35.0	41.0	27.0	41.0
42	35.31587468418642	38.0	35.0	40.0	27.0	41.0
43	35.17666409819306	38.0	35.0	40.0	26.0	41.0
44	35.060929825719704	37.0	35.0	40.0	26.0	41.0
45	34.982882811580524	37.0	35.0	40.0	25.0	41.0
46	34.88676599100364	37.0	35.0	40.0	24.0	41.0
47	34.74334100321381	37.0	35.0	40.0	24.0	41.0
48	34.6706348832069	36.0	35.0	40.0	24.0	41.0
49	34.60664257304464	36.0	35.0	40.0	24.0	41.0
50	34.53648223549882	36.0	34.0	40.0	24.0	41.0
51	34.13053450359497	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	15.0
15	43.0
16	104.0
17	195.0
18	475.0
19	936.0
20	1723.0
21	2791.0
22	4469.0
23	7019.0
24	11811.0
25	21289.0
26	34802.0
27	44632.0
28	47190.0
29	45618.0
30	46183.0
31	50568.0
32	58002.0
33	73328.0
34	163219.0
35	225311.0
36	222596.0
37	221771.0
38	359262.0
39	795041.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.17752239759561	16.788795604588476	23.282140196010488	31.751541801805423
2	36.4856391706031	18.879183184385102	22.65697180652545	21.978205838486343
3	25.222356136430648	20.08785574008742	34.69735821759141	19.99242990589053
4	22.68711275544454	22.559946656671627	33.127445363891475	21.62549522399236
5	21.26954497009075	30.54701106357362	29.926066507746636	18.257377458589
6	76.27283997042497	1.9236486705381042	18.76218709286652	3.041324266170414
7	75.74506580363767	1.7606414978124232	17.504345832737005	4.989946865812906
8	66.4697321310307	3.4200340121129704	22.833880723038305	7.276353133818021
9	39.13771461073272	15.916102361123013	27.24684370954295	17.69933931860131
10	27.160644696443736	15.024258337868146	37.71309343325092	20.102003532437195
11	24.64471612762211	12.433202941264518	41.15711718021786	21.764963750895514
12	21.073321241413417	13.435563777222711	43.396282124202855	22.094832857161016
13	16.808192432940487	16.570017424339042	44.8521104610417	21.76967968167877
14	16.689761058140046	17.000233336053537	44.51563905167963	21.79436655412679
15	20.115700235755533	14.685531483348868	43.860780802306955	21.337987478588648
16	19.324982171731232	18.760136688178143	40.331214171741074	21.583666968349544
17	19.969711421943348	19.661658621562136	39.90415588324668	20.46447407324784
18	20.163884745932304	25.47570413972606	36.23721213855979	18.12319897578185
19	21.360911003004663	21.470730678113938	34.87980322676287	22.288555092118532
20	20.535869164496997	25.417308614201183	35.34922287611906	18.69759934518276
21	18.427930120567897	20.31614779809091	36.60566986106048	24.650252220280716
22	19.688436906792294	22.079700870560824	34.41735495334714	23.814507269299742
23	20.18471685756618	19.789439841741565	37.39917647546096	22.626666825231297
24	23.06389512098003	18.352844300879664	34.347354137286075	24.235906440854233
25	17.864150847452763	19.28914109777847	37.390851832426165	25.455856222342604
26	17.78717865545123	18.080550558263685	37.07299809763453	27.05927268865056
27	21.454204416325652	19.8048998930919	36.636836012323755	22.104059678258697
28	22.610878709130823	20.69916339387905	33.35024233733012	23.33971555966001
29	21.550819485241803	18.161746583923268	35.67761569100894	24.609818239825994
30	26.03193792358798	17.444309983461437	32.51753198528793	24.00622010766265
31	24.817524234758213	17.26506360560384	33.161523089812235	24.755889069825713
32	24.862674145996195	17.040052195101747	32.522616988915104	25.574656669986958
33	20.756738757528574	17.78320087035578	35.85321234852123	25.60684802359442
34	20.93664126488645	19.15717705203476	37.560133243498264	22.34604843958052
35	20.515775198550937	22.809480907246662	34.84974429403132	21.824999600171086
36	20.912118424813507	20.707529045007615	33.66391324655748	24.71643928362141
37	19.595020469190004	24.42278032415258	34.13234870166325	21.84985050499417
38	19.031815309387614	21.69348664345882	32.56132862943159	26.713369417721978
39	20.45955310199574	20.187259359379762	34.658195488043475	24.694992050581025
40	18.091048630268155	19.197118935364273	35.704558008614164	27.007274425753415
41	19.62614561235951	22.167048110285528	31.61818348087362	26.588622796481342
42	19.67925109378838	17.79747168698686	34.355514747945804	28.167762471278955
43	22.09446378431711	18.582448617883713	35.091569022978064	24.231518574821113
44	21.25133737645799	19.056584198023163	35.45104597294368	24.241032452575165
45	21.66551912350941	20.111435394003717	34.04787202850226	24.175173453984613
46	22.151875115591565	18.89398710623516	34.04815908515864	24.905978693014642
47	20.001164629863	19.8785094214045	35.15217898556638	24.96814696316612
48	21.012219181699894	18.04192093393473	32.4645085200466	28.481351364318776
49	20.199479771322466	16.43793035431403	36.982616258971035	26.379973615392473
50	19.758806795697268	16.658758939251843	37.86511043684692	25.717323828203973
51	18.34029582418682	15.669725733768075	37.13738080484945	28.852597637195654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	2861.5
2	5346.0
3	83471.5
4	161597.0
5	93202.5
6	24808.0
7	22084.5
8	19361.0
9	18799.0
10	18237.0
11	17401.5
12	16566.0
13	15701.0
14	14836.0
15	13837.0
16	12838.0
17	11815.0
18	10792.0
19	10191.5
20	9591.0
21	9282.5
22	8974.0
23	8831.0
24	8688.0
25	8461.5
26	9666.0
27	11097.0
28	11745.0
29	12393.0
30	13494.0
31	14595.0
32	16822.0
33	19049.0
34	22144.5
35	25240.0
36	27552.5
37	29865.0
38	32956.5
39	36048.0
40	40228.5
41	44409.0
42	53288.0
43	62167.0
44	74694.0
45	87221.0
46	118336.0
47	149451.0
48	195838.0
49	242225.0
50	261516.5
51	280808.0
52	246306.5
53	211805.0
54	187226.5
55	162648.0
56	147011.5
57	131375.0
58	125694.5
59	120014.0
60	116796.5
61	113579.0
62	107732.0
63	101885.0
64	96778.5
65	91672.0
66	74801.0
67	57930.0
68	47322.5
69	36715.0
70	30646.0
71	24577.0
72	23029.0
73	21481.0
74	18116.5
75	11143.5
76	7535.0
77	5590.5
78	3646.0
79	2891.0
80	2136.0
81	1582.5
82	1029.0
83	796.0
84	563.0
85	374.0
86	185.0
87	137.0
88	89.0
89	85.5
90	82.0
91	49.0
92	16.0
93	14.5
94	13.0
95	7.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2438543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.40656361639255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94228879708173	22.48330908250136
2	9.99966666633557	6.081110012653051
3	3.7075752068020904	3.3820386416456265
4	1.9258081684482606	2.3422883434756185
5	1.2998965487123662	1.9762693551575843
6	1.0034186603739779	1.8306307998322053
7	0.7977441110180434	1.6979659942890863
8	0.7116559544821628	1.7311209642357155
9	0.579065352809483	1.584664873942531
>10	5.631361776053498	32.60295786185024
>50	0.23706945918051386	4.921507730116936
>100	0.1522189706703097	8.189545957898721
>500	0.0063869490834713985	1.2647715232707075
>1k	0.004892131212871709	2.9062184428671944
>5k	6.794626684544041E-4	1.478190613005531
>10k+	2.717850673817616E-4	5.5274098032579495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120404	4.937538521978083	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13366	0.5481141812959625	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	9277	0.38043208588079025	TruSeq Adapter, Index 23 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8277	0.3394239921133234	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	7465	0.3061254199741403	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	5723	0.23468932063121298	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	5032	0.20635272783789335	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	4456	0.18273206582783244	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	4386	0.17986149926410977	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	3677	0.15078676078297573	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	3345	0.13717207365217673	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3302	0.13540872562017567	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	3269	0.13405545852584924	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3260	0.13368638568194205	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	2941	0.1206048037701201	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3156803058219601	0.0
2	0.0	0.0	0.0	1.034511181471887	0.0
3	0.0	0.0	0.0	1.743828179367762	0.0
4	0.0	0.0	0.0	2.262785605995055	0.0
5	0.0	0.0	0.0	4.712814168132364	0.0
6	0.0	0.0	0.0	5.802071154783819	0.0
7	0.0	0.0	0.0	8.617563848576793	0.0
8	0.0	0.0	0.0	11.809305802686277	0.0
9	0.0	0.0	0.0	15.013760265863674	0.0
10	0.0	0.0	0.0	16.755209975792923	0.0
11	0.0	0.0	0.0	18.094411293957087	0.0
12	0.0	0.0	0.0	19.01020404397216	0.0
13	0.0	0.0	0.0	19.509067504653395	0.0
14	0.0	0.0	0.0	19.822943454349584	0.0
15	0.0	0.0	0.0	20.527257464805828	0.0
16	0.0	0.0	0.0	21.30038305660388	0.0
17	0.0	0.0	0.0	22.27141370892373	0.0
18	0.0	0.0	0.0	22.824694910034392	0.0
19	0.0	0.0	0.0	23.456383586428455	0.0
20	4.1008093767466886E-5	0.0	0.0	24.141054719970082	0.0
21	1.2302428130240065E-4	0.0	0.0	24.88026661822244	0.0
22	1.2302428130240065E-4	0.0	0.0	25.54529487484945	0.0
23	1.2302428130240065E-4	0.0	0.0	26.13470420656925	0.0
24	1.6403237506986754E-4	0.0	0.0	26.62265951430834	0.0
25	1.6403237506986754E-4	0.0	0.0	27.106103931733006	0.0
26	1.6403237506986754E-4	0.0	0.0	27.59348512615935	0.0
27	1.6403237506986754E-4	0.0	0.0	28.12347373001009	0.0
28	1.6403237506986754E-4	0.0	0.0	28.619958721252814	0.0
29	1.6403237506986754E-4	0.0	0.0	29.141540665881227	0.0
30	1.6403237506986754E-4	0.0	0.0	29.615717254114443	0.0
31	1.6403237506986754E-4	0.0	0.0	30.084234725407754	0.0
32	1.6403237506986754E-4	0.0	0.0	30.597122954157463	0.0
33	1.6403237506986754E-4	0.0	0.0	31.08864596605432	0.0
34	1.6403237506986754E-4	0.0	0.0	31.61670718949799	0.0
35	1.6403237506986754E-4	0.0	0.0	32.112495043146666	0.0
36	1.6403237506986754E-4	0.0	0.0	32.618452904049676	0.0
37	2.0504046883733444E-4	0.0	0.0	33.251207790881686	0.0
38	2.0504046883733444E-4	0.0	0.0	33.929686702264426	0.0
39	2.0504046883733444E-4	0.0	0.0	34.628546636249595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31760	0.0	44.369488	1
CGGGTAT	145	0.0	43.448273	5
CACGACG	380	0.0	42.631577	25
CGGGATA	235	0.0	42.12766	5
ACGGGTA	170	0.0	41.02941	4
CACTTAC	3465	0.0	40.974026	36
TCGGCGT	1440	0.0	40.468754	4
GTTTTTT	35615	0.0	40.223923	2
CGCACTT	3535	0.0	40.09901	34
CGACGGT	400	0.0	39.9375	27
ACCCGCA	3600	0.0	39.9375	31
CCCGCAC	3625	0.0	39.910347	32
CATGCGG	350	0.0	39.857143	1
ACTTACT	3535	0.0	39.844414	37
CCGCACT	3555	0.0	39.746834	33
TACGCGG	380	0.0	39.67105	1
CTTACTG	3540	0.0	39.661015	38
GCACTTA	3575	0.0	39.650352	35
TCACGAC	415	0.0	39.578316	24
ACGGGAT	495	0.0	39.545452	4
>>END_MODULE