FastQCFastQC Report
Thu 26 May 2016
SRR1043277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043277_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1884009
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT822664.366539650288295No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC106740.5665578030678197No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG77040.40891524403545837No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC66340.35212146014164475No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT54120.28725977423674726Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG35900.19055110670915054No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC34980.18566790286033666No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33720.1789800367195698No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC30640.1626319194865842No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC22780.12091237356084818No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC22560.1197446509013492No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA22370.11873616314996371No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC20320.10785511109554148No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC20260.10753664127931449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG800.045.0000041
GCGATCG453.8562575E-1045.08
CGTTTTT200850.044.2046281
ACGGGTA1800.043.754
CACGACG3700.041.95945725
ACTACGG1400.041.7857171
ACGTAGG1350.041.6666681
TATACGG1350.041.6666681
TTCACGG1200.041.2500041
CCGTAGG1700.041.029411
CGGGTAC3350.040.9701465
TCACGAC3850.040.90909224
CTTACGG1650.040.909091
ACGGGAT2700.040.8333364
CACTTAC30800.040.7629936
AGGGTAC7350.040.7142875
TAACGGG4450.040.4494362
TACGGGA2900.040.344833
CCCGCAC31800.040.25943432
ACCCGCA31800.040.1886831
Produced by FastQC (version 0.11.5)