##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043277_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1884009
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.328100874252726	33.0	31.0	34.0	31.0	34.0
2	32.62749222535561	34.0	31.0	34.0	31.0	34.0
3	32.7319726179652	34.0	31.0	34.0	31.0	34.0
4	36.20674582764732	37.0	37.0	37.0	35.0	37.0
5	36.192923176057015	37.0	37.0	37.0	35.0	37.0
6	36.33925846426423	37.0	37.0	37.0	35.0	37.0
7	36.2919513654128	37.0	37.0	37.0	35.0	37.0
8	36.19813281146746	37.0	37.0	37.0	35.0	37.0
9	37.777487793317334	39.0	38.0	39.0	35.0	39.0
10	37.56631151974327	39.0	37.0	39.0	35.0	39.0
11	37.275396773582294	39.0	37.0	39.0	35.0	39.0
12	37.280618086219334	39.0	37.0	39.0	35.0	39.0
13	37.24425201790437	39.0	37.0	39.0	34.0	39.0
14	38.61805490313475	40.0	38.0	41.0	35.0	41.0
15	38.69102376899473	40.0	38.0	41.0	35.0	41.0
16	38.599484397367526	40.0	38.0	41.0	34.0	41.0
17	38.57987727234849	40.0	38.0	41.0	34.0	41.0
18	38.3675465456906	40.0	37.0	41.0	34.0	41.0
19	38.14644834499198	40.0	37.0	41.0	34.0	41.0
20	37.89873137548706	40.0	35.0	41.0	34.0	41.0
21	37.82243662317961	40.0	35.0	41.0	34.0	41.0
22	37.76449900186252	40.0	35.0	41.0	34.0	41.0
23	37.73023589590071	40.0	35.0	41.0	34.0	41.0
24	37.650700713213155	39.0	35.0	41.0	34.0	41.0
25	37.604582568342295	39.0	35.0	41.0	34.0	41.0
26	37.56117937865477	39.0	35.0	41.0	34.0	41.0
27	37.53168535819096	39.0	35.0	41.0	34.0	41.0
28	37.450151246623555	39.0	35.0	41.0	34.0	41.0
29	37.387456747818085	39.0	35.0	41.0	34.0	41.0
30	37.26262082612132	39.0	35.0	41.0	33.0	41.0
31	37.11363586904309	39.0	35.0	41.0	33.0	41.0
32	36.87586577346499	39.0	35.0	41.0	33.0	41.0
33	36.53302930081544	39.0	35.0	41.0	31.0	41.0
34	36.18855960879168	38.0	35.0	41.0	31.0	41.0
35	36.04908681434112	38.0	35.0	41.0	30.0	41.0
36	35.881161926508845	38.0	35.0	41.0	30.0	41.0
37	35.74337542973521	38.0	35.0	41.0	30.0	41.0
38	35.58274827774177	38.0	35.0	40.0	30.0	41.0
39	35.52858611609605	38.0	35.0	40.0	29.0	41.0
40	35.453999423569634	37.0	35.0	40.0	29.0	41.0
41	35.39781126310968	37.0	35.0	40.0	29.0	41.0
42	35.33204034587945	37.0	35.0	40.0	29.0	41.0
43	35.24294894557298	37.0	35.0	40.0	28.0	41.0
44	35.12317987865239	37.0	35.0	40.0	28.0	41.0
45	35.04907885259571	37.0	35.0	40.0	27.0	41.0
46	34.928217434205465	36.0	35.0	40.0	27.0	41.0
47	34.79106097688493	36.0	35.0	40.0	26.0	41.0
48	34.712856467246176	36.0	35.0	40.0	26.0	41.0
49	34.654161418549485	35.0	34.0	40.0	27.0	41.0
50	34.57991177324524	35.0	34.0	40.0	26.0	41.0
51	34.19981061661595	35.0	34.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	3.0
13	5.0
14	17.0
15	38.0
16	61.0
17	138.0
18	390.0
19	715.0
20	1259.0
21	2141.0
22	3282.0
23	5166.0
24	8712.0
25	15203.0
26	23920.0
27	29892.0
28	31738.0
29	32381.0
30	33799.0
31	37919.0
32	44529.0
33	59146.0
34	138435.0
35	189596.0
36	176780.0
37	184608.0
38	295431.0
39	568615.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.212386989658757	17.223749992701734	23.154241832178084	30.40962118546143
2	33.801855511305945	20.559562082771365	24.29155062422738	21.34703178169531
3	26.506295882875293	21.11635347814156	33.15228324280829	19.225067396174857
4	23.884864668905507	23.603443507966258	31.196772414569146	21.31491940855909
5	22.26114631087219	31.43366087953932	27.670621531001178	18.634571278587313
6	78.72526086658821	2.1021661786116734	16.380919624056997	2.791653330743112
7	78.54739547422544	2.0615612770427316	15.021371978583966	4.36967127014786
8	69.96410314388095	3.398232174050124	20.029415995358832	6.608248686710096
9	42.70929703626681	15.5220065296928	24.865380154765717	16.903316279274673
10	28.72868441711266	16.908783344453237	33.6514846797441	20.71104755869001
11	25.345844950846836	13.241921880415648	38.95804107092907	22.454192097808452
12	21.09618372311385	15.125883156609124	41.108826974818065	22.669106145458965
13	16.95766846124408	18.414774027087983	42.853086158293294	21.77447135337464
14	17.17942960994348	17.564618852670026	42.46163367584762	22.79431786153888
15	20.933021020600222	14.190218836534221	42.278991236241446	22.59776890662412
16	20.72076088808493	17.99933015181987	37.50688027498807	23.773028685107132
17	21.86815455764808	19.18578945217353	36.58958104764892	22.356474942529466
18	21.649578107110955	24.078865865290453	34.248084802142664	20.02347122545593
19	22.525741649854115	20.670548813726477	33.08227296154105	23.721436574878357
20	21.70058635600998	24.49107196409359	34.334814748761815	19.473526931134618
21	20.11779136936182	19.102244203716648	35.53656060029437	25.24340382662715
22	20.547513308057447	21.27059902580083	33.4238318394445	24.758055826697216
23	21.393581453167158	19.518696566736146	36.56723508221033	22.520486897886368
24	24.090808483398966	17.98255740816525	33.40769603542233	24.51893807301345
25	19.00813637302157	19.540140201028763	35.471964305903	25.979759120046666
26	18.515728958831936	18.622734817084208	35.52950118603467	27.33203503804918
27	21.615395680169254	20.79522974677934	34.7817871358364	22.807587437215002
28	21.985245293414206	21.38296579262626	31.512906785477142	25.1188821284824
29	22.674413975729415	19.14672382138302	33.77117625234274	24.40768595054482
30	26.07147842712004	20.945335186827663	30.039771572216477	22.943414813835815
31	26.122592832624473	18.423425790428816	29.455114067926424	25.998867309020284
32	26.924181360067813	19.250279589959497	28.263771563723957	25.561767486248737
33	22.45610291670581	20.690293942332545	31.761366320436895	25.092236820524743
34	22.787629995398113	21.066088325480397	32.02527164148366	24.121010037637824
35	27.536333425158798	21.729991735708268	28.63760204967174	22.096072789461196
36	26.319301022447345	20.973891313682685	30.519174802243516	22.187632861626458
37	26.52089241611903	23.033276380314533	30.35760444881102	20.088226754755418
38	25.007311536197545	19.389026273229057	30.849162610157382	24.754499580416017
39	26.018506281020954	17.97586954202448	34.16130177722081	21.84432239973376
40	21.334611458862458	18.26949871258577	35.94685588020015	24.449033948351627
41	21.56624517186489	21.745490600097984	30.85680588574683	25.831458342290297
42	21.562901238794506	17.70745256524783	32.8397581964842	27.889887999473462
43	24.179873875337112	18.437597697250915	32.7028692538093	24.679659173602676
44	23.30625809112377	19.74051079373825	32.80345263743432	24.149778477703663
45	24.389108544598248	19.767315336604018	31.74225813146328	24.101317987334454
46	23.846329821142042	19.007658668297232	32.0728298007069	25.073181709853827
47	21.718367587415983	20.510889279191343	33.84224809966407	23.92849503372861
48	23.050526828693492	18.93722376060836	31.35335340754742	26.65889600315073
49	22.309766036149508	16.827414306407242	34.63046089482587	26.232358762617373
50	21.45945162682344	17.925073606336277	35.63751553203833	24.977959234801958
51	20.591515220999476	16.214200675262166	34.937731189182216	28.256552914556142
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	465.0
1	3429.5
2	6394.0
3	56590.5
4	106787.0
5	61255.5
6	15724.0
7	13884.5
8	12045.0
9	11892.0
10	11739.0
11	11435.5
12	11132.0
13	10547.0
14	9962.0
15	9373.0
16	8784.0
17	8067.5
18	7351.0
19	7131.5
20	6912.0
21	6337.5
22	5763.0
23	5659.5
24	5556.0
25	5649.5
26	6704.5
27	7666.0
28	8285.0
29	8904.0
30	10167.0
31	11430.0
32	13589.5
33	15749.0
34	18080.5
35	20412.0
36	21964.5
37	23517.0
38	25572.5
39	27628.0
40	31354.5
41	35081.0
42	41633.0
43	48185.0
44	54684.5
45	61184.0
46	70646.5
47	80109.0
48	104500.5
49	128892.0
50	154049.5
51	179207.0
52	183777.0
53	188347.0
54	170818.0
55	153289.0
56	140568.0
57	127847.0
58	117443.0
59	107039.0
60	101501.5
61	95964.0
62	92084.0
63	88204.0
64	83949.0
65	79694.0
66	67193.5
67	54693.0
68	46670.0
69	38647.0
70	32960.0
71	27273.0
72	25325.0
73	23377.0
74	19957.5
75	13086.5
76	9635.0
77	7581.0
78	5527.0
79	4234.5
80	2942.0
81	2147.5
82	1353.0
83	1039.0
84	725.0
85	554.0
86	383.0
87	266.0
88	149.0
89	92.0
90	35.0
91	23.5
92	12.0
93	12.5
94	13.0
95	7.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1884009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.077223131661718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17443800480761	21.567866845314
2	10.397589582255554	6.046660646293722
3	3.7533814898079485	3.2741373323218386
4	1.927151777719226	2.2414488899728195
5	1.158905503337189	1.6848876954523089
6	0.7940519565339156	1.3853295550961529
7	0.6183299086382714	1.2585521705718516
8	0.48418899654038466	1.126309719224012
9	0.43200121172518363	1.1305256063833262
>10	5.724030216950508	37.44366279512605
>50	0.4110215797683941	7.7836621752177635
>100	0.11476203467006255	5.730339289754514
>500	0.005350625093260755	1.091698025930417
>1k	0.0038745905847750303	2.189243763533467
>5k	5.535129406821471E-4	1.0595624360964044
>10k+	3.690086271214314E-4	4.986113053711383
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82266	4.366539650288295	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10674	0.5665578030678197	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7704	0.40891524403545837	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	6634	0.35212146014164475	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	5412	0.28725977423674726	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG	3590	0.19055110670915054	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	3498	0.18566790286033666	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3372	0.1789800367195698	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3064	0.1626319194865842	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2278	0.12091237356084818	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	2256	0.1197446509013492	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2237	0.11873616314996371	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2032	0.10785511109554148	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	2026	0.10753664127931449	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2987777659236235	0.0
2	0.0	0.0	0.0	0.97950699810882	0.0
3	0.0	0.0	0.0	1.6058309700219053	0.0
4	0.0	0.0	0.0	2.020584827354859	0.0
5	0.0	0.0	0.0	4.150457879978281	0.0
6	0.0	0.0	0.0	4.875295181710915	0.0
7	0.0	0.0	0.0	7.019552454367256	0.0
8	0.0	0.0	0.0	9.404944456210135	0.0
9	0.0	0.0	0.0	11.667619422200213	0.0
10	0.0	0.0	0.0	12.998133236093883	0.0
11	0.0	0.0	0.0	14.138308256489221	0.0
12	0.0	0.0	0.0	15.007624698183502	0.0
13	0.0	0.0	0.0	15.445308382284798	0.0
14	0.0	0.0	0.0	15.716856978921014	0.0
15	0.0	0.0	0.0	16.461280174351607	0.0
16	0.0	0.0	0.0	17.28563929365518	0.0
17	0.0	0.0	0.0	18.268596381439792	0.0
18	0.0	0.0	0.0	18.858243246184067	0.0
19	0.0	0.0	0.0	19.51471569403331	0.0
20	0.0	0.0	0.0	20.228247317289885	0.0
21	0.0	0.0	0.0	20.92251151666473	0.0
22	5.307830270449876E-5	0.0	0.0	21.594854377022614	0.0
23	5.307830270449876E-5	0.0	0.0	22.19580692024295	0.0
24	5.307830270449876E-5	0.0	0.0	22.69134595429215	0.0
25	1.0615660540899752E-4	0.0	0.0	23.181948706189832	0.0
26	1.0615660540899752E-4	0.0	0.0	23.676107704368714	0.0
27	1.0615660540899752E-4	0.0	0.0	24.18964028303474	0.0
28	1.0615660540899752E-4	0.0	0.0	24.705242915506243	0.0
29	1.0615660540899752E-4	0.0	0.0	25.23273508778355	0.0
30	1.0615660540899752E-4	0.0	0.0	25.730184940730112	0.0
31	1.0615660540899752E-4	0.0	0.0	26.211976694378848	0.0
32	1.0615660540899752E-4	0.0	0.0	26.73325870523973	0.0
33	1.0615660540899752E-4	0.0	0.0	27.242385784781284	0.0
34	1.0615660540899752E-4	0.0	0.0	27.79700096974059	0.0
35	1.0615660540899752E-4	0.0	0.0	28.276191886556806	0.0
36	1.0615660540899752E-4	0.0	0.0	28.77438483574123	0.0
37	1.0615660540899752E-4	0.0	0.0	29.368702591123505	0.0
38	1.0615660540899752E-4	0.0	0.0	30.020981853059087	0.0
39	1.0615660540899752E-4	0.0	0.0	30.72044772609897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	80	0.0	45.000004	1
GCGATCG	45	3.8562575E-10	45.0	8
CGTTTTT	20085	0.0	44.204628	1
ACGGGTA	180	0.0	43.75	4
CACGACG	370	0.0	41.959457	25
ACTACGG	140	0.0	41.785717	1
ACGTAGG	135	0.0	41.666668	1
TATACGG	135	0.0	41.666668	1
TTCACGG	120	0.0	41.250004	1
CCGTAGG	170	0.0	41.02941	1
CGGGTAC	335	0.0	40.970146	5
TCACGAC	385	0.0	40.909092	24
CTTACGG	165	0.0	40.90909	1
ACGGGAT	270	0.0	40.833336	4
CACTTAC	3080	0.0	40.76299	36
AGGGTAC	735	0.0	40.714287	5
TAACGGG	445	0.0	40.449436	2
TACGGGA	290	0.0	40.34483	3
CCCGCAC	3180	0.0	40.259434	32
ACCCGCA	3180	0.0	40.18868	31
>>END_MODULE