##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043276_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2769989
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.358019472279494	33.0	31.0	34.0	31.0	34.0
2	32.7040345647582	34.0	31.0	34.0	31.0	34.0
3	32.84605101319897	34.0	31.0	34.0	31.0	34.0
4	36.24387028251737	37.0	37.0	37.0	35.0	37.0
5	36.20099538301416	37.0	37.0	37.0	35.0	37.0
6	36.31949477055685	37.0	37.0	37.0	35.0	37.0
7	36.26381115592878	37.0	37.0	37.0	35.0	37.0
8	36.25253241077853	37.0	37.0	37.0	35.0	37.0
9	37.85025788911075	39.0	38.0	39.0	35.0	39.0
10	37.66765427588341	39.0	37.0	39.0	35.0	39.0
11	37.397829377661786	39.0	37.0	39.0	35.0	39.0
12	37.40226766243476	39.0	37.0	39.0	35.0	39.0
13	37.37359426337072	39.0	37.0	39.0	35.0	39.0
14	38.76860738436145	41.0	38.0	41.0	35.0	41.0
15	38.83402461165008	41.0	38.0	41.0	35.0	41.0
16	38.72001296756052	41.0	38.0	41.0	34.0	41.0
17	38.69716594542433	41.0	38.0	41.0	34.0	41.0
18	38.47545134655769	40.0	38.0	41.0	34.0	41.0
19	38.220906653419924	40.0	37.0	41.0	34.0	41.0
20	37.93951383922463	40.0	35.0	41.0	34.0	41.0
21	37.86970020458565	40.0	35.0	41.0	34.0	41.0
22	37.80608226242054	40.0	35.0	41.0	34.0	41.0
23	37.8133584646004	40.0	35.0	41.0	34.0	41.0
24	37.70888801363471	40.0	35.0	41.0	34.0	41.0
25	37.60932083123796	39.0	35.0	41.0	34.0	41.0
26	37.500448557737954	39.0	35.0	41.0	34.0	41.0
27	37.51826162486566	39.0	35.0	41.0	34.0	41.0
28	37.47983331341749	39.0	35.0	41.0	34.0	41.0
29	37.386886012904746	39.0	35.0	41.0	33.0	41.0
30	37.29656976977165	39.0	35.0	41.0	33.0	41.0
31	37.147955100182706	39.0	35.0	41.0	33.0	41.0
32	36.85490411694776	39.0	35.0	41.0	32.0	41.0
33	36.505946774517874	39.0	35.0	41.0	31.0	41.0
34	36.145666643441544	39.0	35.0	41.0	30.0	41.0
35	35.95345938196866	39.0	35.0	41.0	30.0	41.0
36	35.83060438146144	38.0	35.0	41.0	30.0	41.0
37	35.74891055524047	38.0	35.0	41.0	29.0	41.0
38	35.58365863546751	38.0	35.0	41.0	28.0	41.0
39	35.60440817635016	38.0	35.0	41.0	29.0	41.0
40	35.552788837789606	38.0	35.0	41.0	29.0	41.0
41	35.52256453003965	38.0	35.0	41.0	29.0	41.0
42	35.44382739426041	38.0	35.0	41.0	28.0	41.0
43	35.3376778752551	37.0	35.0	40.0	28.0	41.0
44	35.13155720112968	37.0	35.0	40.0	27.0	41.0
45	35.01656179862086	37.0	35.0	40.0	26.0	41.0
46	34.95383916687034	37.0	35.0	40.0	26.0	41.0
47	34.865871669526484	36.0	35.0	40.0	26.0	41.0
48	34.74861416417177	36.0	35.0	40.0	26.0	41.0
49	34.672795451534284	36.0	35.0	40.0	26.0	41.0
50	34.5602960878184	35.0	35.0	40.0	26.0	41.0
51	34.41766880662703	35.0	34.0	40.0	26.0	41.0
52	34.0167246151519	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	8.0
13	13.0
14	28.0
15	53.0
16	109.0
17	252.0
18	569.0
19	1110.0
20	2119.0
21	3464.0
22	5492.0
23	8717.0
24	14722.0
25	26515.0
26	40282.0
27	49263.0
28	50277.0
29	47944.0
30	47953.0
31	52820.0
32	62672.0
33	82541.0
34	186070.0
35	254124.0
36	256612.0
37	280609.0
38	426106.0
39	867589.0
40	1951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.293405497278147	16.395155359822727	22.967419726215518	30.344019416683604
2	35.67960017169744	19.117512741025326	23.377529658060013	21.825357429217227
3	26.955558307271254	20.829505099117725	33.14403053586133	19.07090605774969
4	23.191355633542226	23.59377600416464	31.254023030416366	21.96084533187677
5	22.07654976247198	32.023556772247105	28.01704266695644	17.88285079832447
6	79.04648718821628	2.0860010635421298	16.333494465140475	2.5340172831011243
7	78.40009472961806	2.0379503312107015	15.425151507821871	4.1368034313493665
8	69.85327378556377	3.5747795388357138	20.063617581152847	6.508329094447668
9	42.38309249603518	15.552913748032934	24.87814933561108	17.1858444203208
10	26.729853439851205	15.976742145907439	34.36768160451179	22.92572280972957
11	23.591537728128163	12.730700374622424	40.82442204644134	22.853339850808073
12	19.80246130941314	14.557097519159823	42.71991693829831	22.920524233128724
13	16.454650180921295	17.95100991375778	43.655191410507406	21.93914849481352
14	16.565697553311583	17.301548850916014	43.57742936885309	22.555324226919314
15	20.14968290487796	14.060091935383138	42.8233830531457	22.9668421065932
16	20.232174207189992	17.924222803772867	37.74830874779647	24.09529424124067
17	20.51094787741034	19.847479538727413	37.855023973019385	21.78654861084286
18	21.795754423573523	23.597566632936086	33.95883521559111	20.647843727899282
19	22.29041342763455	19.965566650264677	33.855730113007674	23.888289809093106
20	22.65644376205104	23.00973036355018	34.9361676165501	19.397658257848676
21	20.633619844699744	18.694261962773137	34.50786266660265	26.164255525924474
22	21.527377906554864	21.391709497763348	33.07338043580679	24.007532159875
23	21.40679981039636	19.322134492230838	36.26209345957692	23.00897223779589
24	24.431468861428694	18.16306851760061	33.07554650939047	24.32991611158023
25	20.195278753814545	18.80928047006685	34.85031890018336	26.145121875935246
26	19.746468307274867	18.465307984977557	35.35389490716389	26.434328800583685
27	22.53225554325306	19.61372409782133	35.319887551900024	22.534132807025586
28	23.706556235421875	21.154921553840104	31.52351868545326	23.61500352528476
29	23.24247497011721	18.724117677001605	33.980098837937625	24.05330851494356
30	27.628015851326488	19.045201984556616	30.048386473736898	23.27839569038
31	28.256971417576025	18.365813005033594	29.238888674287157	24.138326903103223
32	26.825196778759768	19.437730619146862	28.54903033910965	25.188042262983718
33	26.068839984563112	19.71744292125348	30.83986976121566	23.37384733296775
34	26.004074384410913	21.38012100409063	30.737414480707326	21.878390130791132
35	28.141808505376737	23.09518196642658	29.531525215443093	19.231484312753587
36	26.63429349358427	23.70698945013861	28.811775064810725	20.84694199146639
37	25.689307791474985	24.294392504807778	31.218571626096708	18.797728077620523
38	22.81070430243586	22.220088238617556	30.537341484027554	24.431865974919035
39	23.766989688406706	20.753367612651168	34.38999216242375	21.089650536518377
40	20.31257163837113	20.924848438026288	35.06071684761203	23.701863075990552
41	22.29081054112489	23.294893950842404	30.251022657490694	24.163272850542008
42	22.04932943777033	18.751229698024073	32.02684198384904	27.172598880356563
43	24.30471745555668	20.20744486710958	32.22168030270156	23.266157374632172
44	24.03782108882021	20.53571331871715	32.086264602494815	23.34020098996783
45	24.83053181799639	20.352716202122103	31.473662891802096	23.34308908807941
46	24.20164845419964	19.965602751491073	31.572544150897354	24.260204643411942
47	21.956043868766265	22.22095466805103	32.42648256003905	23.396518903143658
48	23.080199957472754	20.94427089782667	30.318640254528084	25.656888890172485
49	21.76441855906287	18.536644008333607	34.684181056314664	25.01475637628886
50	21.617017251693056	18.805670347427373	35.343714361320565	24.233598039559002
51	21.03539039324705	17.322704169583343	34.892232424027675	26.74967301314193
52	19.57227988992014	18.774081774331954	36.37848381347363	25.275154522274274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	663.0
1	3985.0
2	7307.0
3	79294.0
4	151281.0
5	87403.0
6	23525.0
7	22164.0
8	20803.0
9	20283.0
10	19763.0
11	19102.5
12	18442.0
13	17546.5
14	15579.5
15	14508.0
16	13759.5
17	13011.0
18	12097.0
19	11183.0
20	10860.5
21	10538.0
22	9976.5
23	9415.0
24	9209.0
25	9003.0
26	10095.0
27	11187.0
28	12270.0
29	13353.0
30	14947.0
31	16541.0
32	18011.5
33	19482.0
34	23055.5
35	26629.0
36	29743.0
37	32857.0
38	35465.0
39	40810.5
40	43548.0
41	52419.5
42	61291.0
43	75697.5
44	90104.0
45	109436.0
46	128768.0
47	160477.0
48	192186.0
49	220773.0
50	249360.0
51	245580.5
52	241801.0
53	212415.0
54	183029.0
55	172201.5
56	161374.0
57	156816.0
58	152258.0
59	151325.5
60	150393.0
61	146903.0
62	143413.0
63	143831.0
64	123718.0
65	103187.0
66	88465.0
67	73743.0
68	62177.5
69	50612.0
70	44237.5
71	37863.0
72	35686.5
73	33510.0
74	27203.5
75	20897.0
76	16253.0
77	11609.0
78	9259.5
79	6910.0
80	4918.5
81	2927.0
82	2157.5
83	1388.0
84	1097.0
85	806.0
86	531.0
87	256.0
88	224.0
89	129.5
90	67.0
91	44.5
92	22.0
93	14.0
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2769989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.85743820129905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99719101877483	21.170870522778387
2	9.151367673870059	5.09867318864402
3	3.262220627601975	2.7263132859727506
4	1.6079951584489418	1.791785030179179
5	0.9909454965721969	1.3802601465807787
6	0.7027156889445813	1.1745515326714175
7	0.6001924196987717	1.170387626845267
8	0.4769008835132869	1.0628189512493045
9	0.41606097456162605	1.0431353598140478
>10	6.216256166709297	39.366423386289306
>50	0.4412490683651995	8.017533381002027
>100	0.1259448471347263	6.04217365381878
>500	0.005610463804729747	1.0669006498614206
>1k	0.0044361806828095655	2.3460692040321347
>5k	5.219036097423019E-4	0.912715641286074
>10k+	3.914277073067264E-4	5.629388438975133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113530	4.098572232597313	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	23754	0.8575485317811731	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	17594	0.6351649771894401	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	7204	0.2600732349478644	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	6391	0.23072293788892304	Illumina Paired End PCR Primer 2 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5873	0.2120225026164364	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5643	0.20371922054564115	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	4432	0.16000063538158454	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	4215	0.15216666925392122	TruSeq Adapter, Index 22 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3709	0.13389944869817175	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	3539	0.12776224021106222	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	3078	0.111119574843077	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2923	0.10552388475188891	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2907	0.10494626512957271	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24068687637387728	0.0
2	0.0	0.0	0.0	0.7629272174005023	0.0
3	0.0	0.0	0.0	1.2693913224926163	0.0
4	0.0	0.0	0.0	1.6196815222009906	0.0
5	0.0	0.0	0.0	3.413154348266365	0.0
6	0.0	0.0	0.0	4.150521897379376	0.0
7	0.0	0.0	0.0	6.259519442134969	0.0
8	0.0	0.0	0.0	8.488409159747565	0.0
9	3.610122639476186E-5	0.0	0.0	10.732533594898753	0.0
10	7.220245278952371E-5	0.0	0.0	11.90376568282401	0.0
11	7.220245278952371E-5	0.0	0.0	12.825141182871123	0.0
12	7.220245278952371E-5	0.0	0.0	13.469114859300886	0.0
13	1.0830367918428556E-4	0.0	0.0	13.802329178924538	0.0
14	1.0830367918428556E-4	0.0	0.0	14.014496086446552	0.0
15	1.0830367918428556E-4	0.0	0.0	14.55532855906648	0.0
16	1.0830367918428556E-4	0.0	0.0	15.138255061662699	0.0
17	1.0830367918428556E-4	0.0	0.0	15.907102880191943	0.0
18	1.0830367918428556E-4	0.0	0.0	16.348259866735933	0.0
19	1.0830367918428556E-4	0.0	0.0	16.83905603957272	0.0
20	1.0830367918428556E-4	0.0	0.0	17.39219903039326	0.0
21	1.0830367918428556E-4	0.0	0.0	17.9446560979123	0.0
22	1.4440490557904743E-4	0.0	0.0	18.47252823025651	0.0
23	1.4440490557904743E-4	0.0	0.0	18.940327921879835	0.0
24	2.1660735836857113E-4	0.0	0.0	19.332820455243684	0.0
25	2.1660735836857113E-4	0.0	0.0	19.724338255494878	0.0
26	2.1660735836857113E-4	0.0	0.0	20.123690021873735	0.0
27	2.1660735836857113E-4	0.0	0.0	20.54412490446713	0.0
28	2.1660735836857113E-4	0.0	0.0	20.949830486691464	0.0
29	2.52708584763333E-4	0.0	0.0	21.36784658711641	0.0
30	2.52708584763333E-4	0.0	0.0	21.75965319717876	0.0
31	2.52708584763333E-4	0.0	0.0	22.143842448471815	0.0
32	2.52708584763333E-4	0.0	0.0	22.58828464661773	0.0
33	2.52708584763333E-4	0.0	0.0	23.006228544589888	0.0
34	2.52708584763333E-4	0.0	0.0	23.487782803469617	0.0
35	2.52708584763333E-4	0.0	0.0	23.889986566733658	0.0
36	2.52708584763333E-4	0.0	0.0	24.31774999828519	0.0
37	2.52708584763333E-4	0.0	0.0	24.814575075929906	0.0
38	2.52708584763333E-4	0.0	0.0	25.368837204768685	0.0
39	2.52708584763333E-4	0.0	0.0	25.94364093142608	0.0
40	2.52708584763333E-4	0.0	0.0	26.49454564621015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31000	0.0	45.369354	1
ACTACGG	150	0.0	44.466663	1
TCACGAC	530	0.0	42.094337	24
CCCCAAC	2680	0.0	41.880596	12
ATAACGG	110	0.0	41.81818	1
TAGTAGG	550	0.0	41.4	1
GCACTTA	5855	0.0	41.364647	35
TCGGCGT	2685	0.0	41.28864	4
CGCACTT	5910	0.0	41.135365	34
CCGCACT	5925	0.0	40.992405	33
GTCCCCC	2735	0.0	40.954292	9
ACCCGCA	6025	0.0	40.92282	31
CGGTCTA	530	0.0	40.79245	30
CAACTTC	2725	0.0	40.682568	15
CTTACTG	5905	0.0	40.663845	38
CATGCGG	425	0.0	40.588234	1
CGACGGT	540	0.0	40.462963	27
CTTAGAG	2675	0.0	40.411213	21
CGTTCAG	2350	0.0	40.323406	38
ATCAACG	6790	0.0	40.275402	14
>>END_MODULE