FastQCFastQC Report
Thu 26 May 2016
SRR1043275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043275_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2717166
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1088884.007410662432844No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC228860.8422746346745101No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG144070.5302215617301262No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC68120.25070238623624763TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT60700.22339452208661523Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC55730.2051034055335596No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC52230.19222233753844997No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG42870.15777468141438541No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC41270.1518861931880496TruSeq Adapter, Index 22 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC36740.1352144108972363No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC32570.11986753845734859No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT30460.11210209460886822No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29590.10890022913579811No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA27690.10190764936702432No Hit
GGGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC27400.10084036087600096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT303000.044.2797051
CGCACTT57650.041.2922834
GCACTTA57750.041.2207835
CTTACTG57500.040.93043538
CCGGTAT2050.040.6097642
CTTCTTA21300.040.56337718
ACCCGCA59400.040.53030431
ATAACGG1000.040.5000041
CCGCACT58750.040.4808533
CACGACG4450.040.44943625
CGGTCTA4450.040.44943630
CCAGTAG21450.040.38461325
CACTTAC58900.040.37775836
TAGCATA20750.040.1204829
CCCGCAC60200.040.1038232
GTTGATC22050.040.015
ACTTACT59050.039.93226237
GCGTAGG4700.039.7340431
ACCGGTA2100.039.6428641
CATATGC20900.039.61722632