FastQCFastQC Report
Thu 26 May 2016
SRR1043274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043274_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2221206
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT849193.823103305141441No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC139380.6274969543572275No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA99980.45011583797270494No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT90850.40901204120644374RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT76620.3449477446036073TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT54290.24441677178973945RNA PCR Primer, Index 35 (96% over 25bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38430.17301411935678185No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC36340.16360481648257746No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC35210.15851749004819904TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG29980.1349717225687307No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25840.11633319917198136No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC24210.10899484334186023No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT22310.10044093163803808RNA PCR Primer, Index 35 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA253.4181925E-546.017
CGACACT206.312733E-446.035
CTACGTT206.312733E-446.043
TTACGCA253.4181925E-546.018
CGTTTTT220800.045.093751
ACTACGG900.043.4444471
CTTGCGG6550.043.190841
GCGATAT800.043.1258
ATAACGG800.043.1251
TAGGGTA9500.042.8526344
AGGGCGA10550.042.511855
TATCCGG1200.042.1666681
TAGTAGG5000.041.861
ACTTACT38250.041.85098337
CGCACTT38900.041.80205534
CACTTAC38600.041.76943236
CCGCACT38900.041.74292833
CCCGCAC39850.041.4981232
ACCCGCA39900.041.44611431
GTAGGGT9250.041.2756773
Produced by FastQC (version 0.11.5)