##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043274_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2221206
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35342692213149	33.0	31.0	34.0	31.0	34.0
2	32.6842021856595	34.0	31.0	34.0	31.0	34.0
3	32.811697339193216	34.0	31.0	34.0	31.0	34.0
4	36.230043498892044	37.0	37.0	37.0	35.0	37.0
5	36.17502473881306	37.0	37.0	37.0	35.0	37.0
6	36.32913156186324	37.0	37.0	37.0	35.0	37.0
7	36.27009651513637	37.0	37.0	37.0	35.0	37.0
8	36.23972472611725	37.0	37.0	37.0	35.0	37.0
9	37.81520984546233	39.0	38.0	39.0	35.0	39.0
10	37.64885832291107	39.0	37.0	39.0	35.0	39.0
11	37.34777998978933	39.0	37.0	39.0	35.0	39.0
12	37.35581526432038	39.0	37.0	39.0	35.0	39.0
13	37.34547358507045	39.0	37.0	39.0	35.0	39.0
14	38.69939483325725	40.0	38.0	41.0	35.0	41.0
15	38.749092610050575	41.0	38.0	41.0	35.0	41.0
16	38.64649294122202	40.0	38.0	41.0	34.0	41.0
17	38.605318011926855	40.0	38.0	41.0	34.0	41.0
18	38.40918086841113	40.0	37.0	41.0	34.0	41.0
19	38.2045735514851	40.0	37.0	41.0	34.0	41.0
20	37.95477231738074	40.0	35.0	41.0	34.0	41.0
21	37.86255934839002	40.0	35.0	41.0	34.0	41.0
22	37.813389663092934	40.0	35.0	41.0	34.0	41.0
23	37.827955173901024	40.0	35.0	41.0	34.0	41.0
24	37.74692171730132	40.0	35.0	41.0	34.0	41.0
25	37.63161588794556	40.0	35.0	41.0	34.0	41.0
26	37.53891219454657	39.0	35.0	41.0	34.0	41.0
27	37.560119142483856	39.0	35.0	41.0	34.0	41.0
28	37.47401681789082	39.0	35.0	41.0	33.0	41.0
29	37.40443254700374	39.0	35.0	41.0	33.0	41.0
30	37.35948219120604	39.0	35.0	41.0	33.0	41.0
31	37.15804567428685	39.0	35.0	41.0	33.0	41.0
32	36.97283817889921	39.0	35.0	41.0	33.0	41.0
33	36.631838289649856	39.0	35.0	41.0	31.0	41.0
34	36.342922718559194	39.0	35.0	41.0	31.0	41.0
35	36.16009861309577	39.0	35.0	41.0	30.0	41.0
36	36.18149014544351	39.0	35.0	41.0	30.0	41.0
37	36.13164245009243	39.0	35.0	41.0	30.0	41.0
38	36.04204292623017	39.0	35.0	41.0	30.0	41.0
39	36.02463616611877	39.0	35.0	41.0	30.0	41.0
40	35.964519274664305	38.0	35.0	41.0	30.0	41.0
41	35.90867573741472	38.0	35.0	41.0	30.0	41.0
42	35.788775557062245	38.0	35.0	41.0	30.0	41.0
43	35.63928424468509	38.0	35.0	41.0	30.0	41.0
44	35.4587179217056	37.0	35.0	40.0	29.0	41.0
45	35.35377042921728	37.0	35.0	40.0	28.0	41.0
46	35.2761220706229	37.0	35.0	40.0	27.0	41.0
47	35.187128523873966	37.0	35.0	40.0	27.0	41.0
48	35.08343800620023	37.0	35.0	40.0	27.0	41.0
49	35.038189614110536	36.0	35.0	40.0	27.0	41.0
50	34.93222375592359	36.0	35.0	40.0	27.0	41.0
51	34.794918166077345	36.0	35.0	40.0	27.0	41.0
52	34.43378056785368	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	1.0
13	3.0
14	9.0
15	38.0
16	84.0
17	176.0
18	450.0
19	860.0
20	1634.0
21	2579.0
22	4024.0
23	6348.0
24	11033.0
25	18952.0
26	28505.0
27	34292.0
28	35907.0
29	35576.0
30	38043.0
31	43457.0
32	51565.0
33	66638.0
34	149386.0
35	210218.0
36	197449.0
37	213501.0
38	332124.0
39	736793.0
40	1557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.519321485715416	17.62407448926394	23.590607985031557	28.265996039989087
2	34.41423262858105	20.411659251775838	24.642649083425848	20.53145903621726
3	26.865675673485484	20.98094458595916	32.996714397493975	19.156665343061384
4	24.085699390331197	23.377750645370128	30.897584465376017	21.63896549892266
5	21.542171234905723	31.793088979590365	27.811648266752385	18.853091518751526
6	78.60387555229006	2.242385442863021	16.26895479302685	2.8847842118200653
7	78.67001079593699	2.2501289839843763	14.723217927558272	4.35664229252037
8	69.38550499143258	3.8868074370409587	19.92683254052078	6.800855031005678
9	40.80215882723169	16.740725533786602	24.778386155989136	17.678729482992573
10	28.041928573936865	17.024805443529324	34.01764626963911	20.915619712894706
11	24.804588138155577	13.935177556696676	39.149948271344485	22.11028603380326
12	21.22630679009511	15.69921925296438	41.26789680921085	21.806577147729655
13	16.59850549656358	19.24134906892922	42.28495691079531	21.875188523711895
14	16.78354011289363	18.882985189126988	42.43906238322785	21.894412314751534
15	20.396307231296873	14.878854099979922	42.72057611945943	22.00426254926378
16	20.239635585353184	18.917380918293937	36.67953355069273	24.16344994566015
17	20.511379854007235	20.227885211907406	36.6766972536541	22.584037680431262
18	21.7082071631357	24.791487147072356	33.783719294833524	19.716586394958416
19	22.83957453743597	20.904769751207226	32.63434368536732	23.621312025989486
20	23.001558612753612	24.078901281556057	33.48311682932605	19.43642327636428
21	19.951098637406886	20.04982878670416	34.38577961701886	25.613292958870094
22	21.083411444053365	21.788298789036226	32.52377312144844	24.604516645461967
23	21.759215489243232	19.685702271648825	36.32639205908862	22.228690180019324
24	24.589344707334664	18.280564702238333	32.7758433931837	24.3542471972433
25	19.574321337147477	20.541228503794787	34.12677617474471	25.75767398431303
26	19.55977968725098	19.455737108579754	34.78758836415893	26.196894840010337
27	22.9708995923836	20.57602941825297	34.88757908991782	21.565491899445615
28	22.183579550928638	21.61645520496523	32.434002069146224	23.765963174959907
29	24.27523606545273	19.10165018462943	35.03187007418492	21.591243675732912
30	27.350682467092202	19.483919996614453	31.894295261222954	21.27110227507039
31	27.68572568235454	18.59359285001031	32.03268854847322	21.687992919161932
32	27.0630459309042	18.764581042910926	31.46835547896053	22.704017547224346
33	23.995208008622342	19.313742174296305	35.4776639357178	21.213385881363546
34	25.251147349683013	18.86668773630181	34.362413931891055	21.519750982124126
35	23.598666670268315	21.43353655626718	35.8738000887806	19.093996684683905
36	24.973370322248364	20.66818656171467	32.15410907407958	22.204334041957388
37	24.60267080135746	22.216579641870226	33.66063300747432	19.520116549297995
38	22.362761490829755	20.164721327062864	31.302094447790974	26.1704227343164
39	24.521993907814043	17.771516914685083	34.986219198039265	22.72026997946161
40	20.417241804677282	18.14428738262007	36.00471995843699	25.433750854265657
41	22.26736286503818	22.662643626930596	30.12872286496615	24.941270643065074
42	21.829582668154146	17.530251584049385	33.43534998554839	27.204815762248074
43	23.426868106785236	18.783399648659334	33.79191304183403	23.997819202721406
44	22.698164870795416	19.59372521053878	33.65964255454019	24.048467364125614
45	24.20171744538778	19.186739095788504	33.257113477993485	23.354429980830233
46	24.31057722696589	19.31729880074158	32.93818763320466	23.433936339087865
47	20.95163618322659	19.927237725812013	35.278222731255006	23.842903359706394
48	21.078594241146476	19.205287578009422	33.09918125558818	26.616936925255917
49	22.003227075741737	17.118493286980137	36.710642776941896	24.16763686033623
50	20.75822773754438	17.235366733207094	36.753367314873095	25.253038214375433
51	20.20001746798811	16.173286043707787	36.28060612117922	27.346090367124887
52	18.8148240190239	16.86669313877236	38.70086790689382	25.617614935309916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	638.0
1	4355.0
2	8072.0
3	61197.5
4	114323.0
5	65487.5
6	16652.0
7	15368.5
8	14085.0
9	13781.0
10	13477.0
11	12978.5
12	12480.0
13	11730.5
14	10447.5
15	9914.0
16	9222.5
17	8531.0
18	8020.0
19	7509.0
20	7144.5
21	6780.0
22	7289.5
23	7799.0
24	7561.0
25	7323.0
26	8460.0
27	9597.0
28	10012.5
29	10428.0
30	11077.0
31	11726.0
32	14105.5
33	16485.0
34	19921.0
35	23357.0
36	26368.5
37	29380.0
38	31226.5
39	37066.0
40	41059.0
41	47787.5
42	54516.0
43	69711.5
44	84907.0
45	102667.5
46	120428.0
47	152063.5
48	183699.0
49	207403.5
50	231108.0
51	217193.0
52	203278.0
53	180013.5
54	156749.0
55	145379.0
56	134009.0
57	125714.0
58	117419.0
59	112544.0
60	107669.0
61	104548.5
62	101428.0
63	100351.0
64	83270.5
65	67267.0
66	56321.5
67	45376.0
68	37493.0
69	29610.0
70	26367.5
71	23125.0
72	22021.5
73	20918.0
74	16876.0
75	12834.0
76	10335.0
77	7836.0
78	5563.5
79	3291.0
80	2364.5
81	1438.0
82	1101.5
83	765.0
84	580.5
85	396.0
86	262.5
87	129.0
88	84.5
89	26.5
90	13.0
91	8.5
92	4.0
93	3.5
94	3.0
95	4.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2221206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.281125536078953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19833326380895	20.701312520655247
2	11.334574918233846	6.411090723213266
3	4.2119628819488675	3.573571530531025
4	2.0529483626877503	2.3223876145704003
5	1.2201247314483108	1.7253250349882148
6	0.7731743514726055	1.3119744537163915
7	0.5586502408451478	1.1059480314471657
8	0.4364620297999725	0.9874909965203885
9	0.35544470541992823	0.9047138701604035
>10	5.165998810192001	34.71238598678889
>50	0.545248333210474	10.046573656852754
>100	0.13476788487225352	6.657846498622061
>500	0.00671426512362313	1.2720166755303732
>1k	0.004636040204406448	2.343295691940261
>5k	6.39453821297441E-4	1.4546246496717339
>10k+	3.197269106487205E-4	4.469442064791402
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84919	3.823103305141441	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13938	0.6274969543572275	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9998	0.45011583797270494	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	9085	0.40901204120644374	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT	7662	0.3449477446036073	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	5429	0.24441677178973945	RNA PCR Primer, Index 35 (96% over 25bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3843	0.17301411935678185	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3634	0.16360481648257746	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	3521	0.15851749004819904	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2998	0.1349717225687307	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2584	0.11633319917198136	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2421	0.10899484334186023	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	2231	0.10044093163803808	RNA PCR Primer, Index 35 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3594893945001049	0.0
2	0.0	0.0	0.0	1.1977277208867616	0.0
3	0.0	0.0	0.0	1.885147077758659	0.0
4	0.0	0.0	0.0	2.3308508981157083	0.0
5	0.0	0.0	0.0	4.711404525289415	0.0
6	0.0	0.0	0.0	5.3280965385470775	0.0
7	0.0	0.0	0.0	7.31818660673526	0.0
8	0.0	0.0	0.0	9.586098722946003	0.0
9	4.5020587914853464E-5	0.0	0.0	11.574793152908825	0.0
10	4.5020587914853464E-5	0.0	0.0	12.827671093991283	0.0
11	4.5020587914853464E-5	0.0	0.0	14.014773956130139	0.0
12	4.5020587914853464E-5	0.0	0.0	14.900959208646114	0.0
13	4.5020587914853464E-5	0.0	0.0	15.323747549754502	0.0
14	4.5020587914853464E-5	0.0	0.0	15.609133056546758	0.0
15	4.5020587914853464E-5	0.0	0.0	16.43962784181206	0.0
16	4.5020587914853464E-5	0.0	0.0	17.381368499814965	0.0
17	4.5020587914853464E-5	0.0	0.0	18.51975908582995	0.0
18	4.5020587914853464E-5	0.0	0.0	19.173277940001963	0.0
19	4.5020587914853464E-5	0.0	0.0	19.896533684854084	0.0
20	4.5020587914853464E-5	0.0	0.0	20.688265743924696	0.0
21	9.004117582970693E-5	0.0	0.0	21.458703064911585	0.0
22	1.8008235165941386E-4	0.0	0.0	22.22058647419465	0.0
23	1.8008235165941386E-4	0.0	0.0	22.870368619569728	0.0
24	3.601647033188277E-4	0.0	0.0	23.4231313979883	0.0
25	3.601647033188277E-4	0.0	0.0	23.937041409036354	0.0
26	3.601647033188277E-4	0.0	0.0	24.442307467204753	0.0
27	3.601647033188277E-4	0.0	0.0	25.002948848508424	0.0
28	4.95226467063388E-4	0.0	0.0	25.558007676910652	0.0
29	4.95226467063388E-4	0.0	0.0	26.128598608143506	0.0
30	4.95226467063388E-4	0.0	0.0	26.67168196016038	0.0
31	4.95226467063388E-4	0.0	0.0	27.199818476989527	0.0
32	4.95226467063388E-4	0.0	0.0	27.79062365219615	0.0
33	4.95226467063388E-4	0.0	0.0	28.338794330647406	0.0
34	4.95226467063388E-4	0.0	0.0	28.964085276196805	0.0
35	4.95226467063388E-4	0.0	0.0	29.49537323417999	0.0
36	4.95226467063388E-4	0.0	0.0	30.058310665467317	0.0
37	4.95226467063388E-4	0.0	0.0	30.67797403752736	0.0
38	4.95226467063388E-4	0.0	0.0	31.299483253691914	0.0
39	4.95226467063388E-4	0.0	0.0	32.00788220453213	0.0
40	5.402470549782416E-4	0.0	0.0	32.72681597294443	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	25	3.4181925E-5	46.0	17
CGACACT	20	6.312733E-4	46.0	35
CTACGTT	20	6.312733E-4	46.0	43
TTACGCA	25	3.4181925E-5	46.0	18
CGTTTTT	22080	0.0	45.09375	1
ACTACGG	90	0.0	43.444447	1
CTTGCGG	655	0.0	43.19084	1
GCGATAT	80	0.0	43.125	8
ATAACGG	80	0.0	43.125	1
TAGGGTA	950	0.0	42.852634	4
AGGGCGA	1055	0.0	42.51185	5
TATCCGG	120	0.0	42.166668	1
TAGTAGG	500	0.0	41.86	1
ACTTACT	3825	0.0	41.850983	37
CGCACTT	3890	0.0	41.802055	34
CACTTAC	3860	0.0	41.769432	36
CCGCACT	3890	0.0	41.742928	33
CCCGCAC	3985	0.0	41.49812	32
ACCCGCA	3990	0.0	41.446114	31
GTAGGGT	925	0.0	41.275677	3
>>END_MODULE