##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043273_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2174697
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34191567836807	33.0	31.0	34.0	31.0	34.0
2	32.622478441824306	34.0	31.0	34.0	31.0	34.0
3	32.73002032007218	34.0	31.0	34.0	31.0	34.0
4	36.19937168258382	37.0	37.0	37.0	35.0	37.0
5	36.191501160851374	37.0	37.0	37.0	35.0	37.0
6	36.3467614109	37.0	37.0	37.0	35.0	37.0
7	36.298964867289556	37.0	37.0	37.0	35.0	37.0
8	36.19269029202689	37.0	37.0	37.0	35.0	37.0
9	37.77556367622708	39.0	38.0	39.0	35.0	39.0
10	37.59048180045312	39.0	37.0	39.0	35.0	39.0
11	37.33795144794884	39.0	37.0	39.0	35.0	39.0
12	37.35526420462253	39.0	37.0	39.0	35.0	39.0
13	37.35528121848699	39.0	37.0	39.0	35.0	39.0
14	38.71056243697398	40.0	38.0	41.0	35.0	41.0
15	38.767567159930785	40.0	38.0	41.0	35.0	41.0
16	38.70165177033858	40.0	38.0	41.0	35.0	41.0
17	38.64085433510967	40.0	38.0	41.0	34.0	41.0
18	38.46175904045483	40.0	38.0	41.0	34.0	41.0
19	38.27538503065025	40.0	37.0	41.0	34.0	41.0
20	38.04827247198116	40.0	35.0	41.0	34.0	41.0
21	37.95834362212299	40.0	35.0	41.0	34.0	41.0
22	37.92323988123403	40.0	35.0	41.0	34.0	41.0
23	37.90331342711192	40.0	35.0	41.0	34.0	41.0
24	37.821965082951785	40.0	35.0	41.0	34.0	41.0
25	37.777242990632715	40.0	35.0	41.0	34.0	41.0
26	37.749433139421264	40.0	35.0	41.0	34.0	41.0
27	37.71941700384008	40.0	35.0	41.0	34.0	41.0
28	37.60595338109171	39.0	35.0	41.0	34.0	41.0
29	37.553509293478584	39.0	35.0	41.0	34.0	41.0
30	37.460417704167526	40.0	35.0	41.0	33.0	41.0
31	37.256848655237945	39.0	35.0	41.0	33.0	41.0
32	37.07962948401548	39.0	35.0	41.0	33.0	41.0
33	36.73041531762816	39.0	35.0	41.0	32.0	41.0
34	36.420103122411994	39.0	35.0	41.0	31.0	41.0
35	36.24070801587531	39.0	35.0	41.0	31.0	41.0
36	36.23079583040764	39.0	35.0	41.0	31.0	41.0
37	36.13297254743994	39.0	35.0	41.0	31.0	41.0
38	36.03926432050074	39.0	35.0	41.0	30.0	41.0
39	35.98247709910852	38.0	35.0	41.0	30.0	41.0
40	35.896392003115835	38.0	35.0	41.0	30.0	41.0
41	35.797865633695174	38.0	35.0	41.0	30.0	41.0
42	35.66788706656605	38.0	35.0	40.0	30.0	41.0
43	35.51348578675558	38.0	35.0	40.0	29.0	41.0
44	35.38505824029738	37.0	35.0	40.0	28.0	41.0
45	35.3157823825572	37.0	35.0	40.0	28.0	41.0
46	35.1916455487822	37.0	35.0	40.0	27.0	41.0
47	35.04728152933489	37.0	35.0	40.0	27.0	41.0
48	34.97322155684217	36.0	35.0	40.0	27.0	41.0
49	34.920281767988826	36.0	35.0	40.0	27.0	41.0
50	34.84438245879771	36.0	35.0	40.0	27.0	41.0
51	34.47149970777538	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	8.0
14	6.0
15	38.0
16	68.0
17	164.0
18	384.0
19	715.0
20	1398.0
21	2235.0
22	3605.0
23	5804.0
24	9341.0
25	16096.0
26	25411.0
27	33277.0
28	35668.0
29	35823.0
30	37527.0
31	41612.0
32	48938.0
33	64019.0
34	143763.0
35	208780.0
36	189163.0
37	208558.0
38	342573.0
39	719571.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.76833232399732	17.65859795640496	23.640902617698007	27.93216710189971
2	34.16977169693065	20.398014068166738	24.777520730474176	20.654693504428433
3	26.871605561602372	21.085466159193672	32.73660652495497	19.306321754248984
4	24.175643779340295	23.449197750307285	30.633233043499853	21.741925426852568
5	21.693550871684653	31.8260888758296	27.489576708847256	18.990783543638493
6	78.90124463316039	2.223620118113006	15.985491312123024	2.8896439366035818
7	79.04659821575144	2.226057239238386	14.383061180477096	4.344283364533083
8	69.73624371579122	3.8274297522827316	19.625170770916593	6.811155761009465
9	40.9622122070339	16.76334680187631	24.461476702271625	17.81296428881817
10	28.25933911712758	17.065135970666258	33.692325873443515	20.983199038762642
11	24.976077127066436	13.908328378620102	38.96827925913357	22.14731523517989
12	21.388312946585202	15.71878749085505	41.016196739131935	21.876702823427816
13	16.692210455065695	19.22718429280033	42.16964478269846	21.910960469435512
14	16.904469910061035	18.82023104827937	42.336288687573486	21.939010354086108
15	20.505155430848525	14.875037763881588	42.51718745186111	22.102619353408773
16	20.290964672319866	18.865662664729847	36.48117415897479	24.362198503975495
17	20.806806649386097	20.009684107717074	36.48425504794461	22.699254194952218
18	21.8267188486488	24.80842158700729	33.595300862602926	19.769558701740976
19	22.997548624015206	20.938549140408984	32.328963529172114	23.734938706403696
20	23.090894961459	24.173620508972053	33.20209665990251	19.53338786966644
21	20.01791513944241	20.121699712649622	34.191797753893994	25.66858739401397
22	21.21596709794514	21.746155901258888	32.27309367695821	24.764783323837758
23	21.93234275855441	19.7461991256713	36.00285465055592	22.318603465218374
24	24.713741730457162	18.272200678991144	32.546649027427726	24.467408563123964
25	19.57518679613758	20.58470674305432	33.965513356573354	25.87459310423475
26	19.584889297221636	19.302735047687104	34.65425298328917	26.45812267180209
27	22.99483560238507	20.609721722152557	34.73403421258226	21.661408462880118
28	22.27827600810596	21.62232255803912	32.20568198696186	23.893719446893062
29	24.34031039726454	19.1352634412978	34.78820267835013	21.73622348308753
30	27.4612509236919	19.47549474708431	31.622520286734197	21.4407340424896
31	27.811690548154523	18.51209616787994	31.848712717219918	21.82750056674562
32	27.16005953932893	18.655610413772585	31.30983304800623	22.874496998892262
33	24.166171195343537	19.12946953069784	35.35577600005886	21.348583273899767
34	25.43936005797589	18.825932992044407	34.17409413817189	21.560612811807804
35	23.743997439643316	21.337777170796667	35.7511414233799	19.167083966180115
36	25.028544206388293	20.612480727200158	32.101207662492754	22.2577674039188
37	24.708361670614345	22.081099114037496	33.62243107890433	19.588108136443836
38	22.540979272054912	20.094109662173626	31.247985351522534	26.11692571424893
39	24.61864802314989	17.669817910265202	34.91244987232704	22.799084194257865
40	20.535734403459426	18.035570012742006	35.91387673777082	25.514818846027747
41	22.366242285706928	22.53831223384223	30.05485361868803	25.040591861762813
42	21.93896437066865	17.386835959216388	33.45192456696266	27.2222751031523
43	23.62568210651875	18.58111727748739	33.723134763141715	24.070065852852142
44	22.841526888573444	19.41235951491173	33.63075407746459	24.115359519050237
45	24.33037797909318	18.962733658987897	33.28215378970036	23.42473457221857
46	24.470995269685847	19.201157678517973	32.92720779032665	23.40063926146953
47	21.18281305395648	19.681316523635246	35.25525624949131	23.88061417291696
48	21.247143854983015	19.02117858257955	33.05343227125434	26.678245291183096
49	22.13356619335935	16.960707629614607	36.623216935508715	24.282509241517324
50	20.90829205172031	17.0480761227886	36.789906823801196	25.25372500168989
51	20.300667173403927	16.142846566671125	36.16278497648178	27.393701283443168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	541.0
1	4116.0
2	7691.0
3	58290.5
4	108890.0
5	62968.0
6	17046.0
7	15414.0
8	13782.0
9	13306.0
10	12830.0
11	12279.0
12	11728.0
13	11168.0
14	10608.0
15	9875.5
16	9143.0
17	8582.0
18	8021.0
19	7343.0
20	6665.0
21	6593.0
22	6521.0
23	6898.5
24	7276.0
25	7302.5
26	8176.5
27	9024.0
28	9617.5
29	10211.0
30	11629.0
31	13047.0
32	15263.5
33	17480.0
34	21722.0
35	25964.0
36	28028.5
37	30093.0
38	32285.0
39	34477.0
40	41570.5
41	48664.0
42	55645.5
43	62627.0
44	75523.0
45	88419.0
46	113773.5
47	139128.0
48	178611.5
49	218095.0
50	227603.5
51	237112.0
52	207324.5
53	177537.0
54	159869.0
55	142201.0
56	133497.5
57	124794.0
58	119883.0
59	114972.0
60	109961.0
61	104950.0
62	100203.0
63	95456.0
64	90372.5
65	85289.0
66	69333.0
67	53377.0
68	44032.5
69	34688.0
70	30155.5
71	25623.0
72	23596.5
73	21570.0
74	18401.0
75	12175.0
76	9118.0
77	6617.0
78	4116.0
79	2907.0
80	1698.0
81	1306.5
82	915.0
83	731.0
84	547.0
85	334.0
86	121.0
87	89.0
88	57.0
89	34.0
90	11.0
91	9.5
92	8.0
93	5.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2174697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.90609181266919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73461233238346	20.297387697063932
2	11.28012000767141	6.2956812918401
3	4.30067882628135	3.600454145490291
4	2.102363988834543	2.346750499842647
5	1.268158669772649	1.7694676135853984
6	0.8076104083356226	1.3522350122328681
7	0.5884718150723166	1.1495363950403306
8	0.4625319372318063	1.0325966965346023
9	0.3872571997761939	0.9726151474864475
>10	5.391037047471832	35.574677679211945
>50	0.5278055557508686	9.598898380798346
>100	0.13656027708655044	6.605364451028445
>500	0.0071435477434931526	1.3323897365008273
>1k	0.004651612484135076	2.2953820679759556
>5k	6.645160691621536E-4	1.392194286260259
>10k+	3.322580345810768E-4	4.3843688991077325
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81014	3.7253005821040817	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13558	0.62344317392262	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	8728	0.40134326759084143	RNA PCR Primer, Index 10 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8494	0.39058314790520243	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	7516	0.34561136562932676	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	5292	0.2433442451982966	RNA PCR Primer, Index 35 (95% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3590	0.16508046868138412	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	3554	0.16342506565282428	TruSeq Adapter, Index 10 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3445	0.15841287314968477	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	2804	0.1289375025578276	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2560	0.11771754869758869	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2342	0.10769316369130964	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2203	0.10130146866437026	RNA PCR Primer, Index 35 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35830278884828554	0.0
2	0.0	0.0	0.0	1.1917982137281653	0.0
3	0.0	0.0	0.0	1.8842624972582387	0.0
4	0.0	0.0	0.0	2.329014110931316	0.0
5	0.0	0.0	0.0	4.736429948631924	0.0
6	0.0	0.0	0.0	5.3567922335847244	0.0
7	0.0	0.0	0.0	7.354495821716773	0.0
8	0.0	0.0	0.0	9.65739135153081	0.0
9	0.0	0.0	0.0	11.707378085314874	0.0
10	0.0	0.0	0.0	13.009720434616868	0.0
11	0.0	0.0	0.0	14.211267132846553	0.0
12	0.0	0.0	0.0	15.113875634168807	0.0
13	0.0	0.0	0.0	15.547637211068944	0.0
14	0.0	0.0	0.0	15.845839673297016	0.0
15	0.0	0.0	0.0	16.697406581238674	0.0
16	0.0	0.0	0.0	17.66719685546998	0.0
17	0.0	0.0	0.0	18.82078284928889	0.0
18	0.0	0.0	0.0	19.480368989335066	0.0
19	0.0	0.0	0.0	20.227139688885394	0.0
20	0.0	0.0	0.0	21.041092161344775	0.0
21	0.0	0.0	0.0	21.82851220192974	0.0
22	1.3795025237998672E-4	0.0	0.0	22.606597608770326	0.0
23	1.3795025237998672E-4	0.0	0.0	23.283197613276702	0.0
24	3.2188392221996903E-4	0.0	0.0	23.841850151998187	0.0
25	3.2188392221996903E-4	0.0	0.0	24.369877734691315	0.0
26	3.2188392221996903E-4	0.0	0.0	24.892663207794005	0.0
27	3.2188392221996903E-4	0.0	0.0	25.46819166072331	0.0
28	3.678673396799646E-4	0.0	0.0	26.026476332105116	0.0
29	3.678673396799646E-4	0.0	0.0	26.61161531928356	0.0
30	3.678673396799646E-4	0.0	0.0	27.156104965427367	0.0
31	3.678673396799646E-4	0.0	0.0	27.692731447185515	0.0
32	3.678673396799646E-4	0.0	0.0	28.291251608844817	0.0
33	3.678673396799646E-4	0.0	0.0	28.839925745977485	0.0
34	3.678673396799646E-4	0.0	0.0	29.459276395746166	0.0
35	3.678673396799646E-4	0.0	0.0	29.996822545853515	0.0
36	3.678673396799646E-4	0.0	0.0	30.555521067992462	0.0
37	3.678673396799646E-4	0.0	0.0	31.177952606730962	0.0
38	3.678673396799646E-4	0.0	0.0	31.80341905102182	0.0
39	3.678673396799646E-4	0.0	0.0	32.51901299353427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAT	25	3.8919574E-5	45.000004	22
GACCGTA	20	7.034678E-4	45.0	15
CGATTCG	20	7.034678E-4	45.0	9
CACCGTA	20	7.034678E-4	45.0	37
AATACGG	45	3.8562575E-10	45.0	1
CGTTTTT	20500	0.0	44.341465	1
ACGTAGG	155	0.0	43.548386	1
GCGATAT	100	0.0	42.750004	8
TCTACGG	150	0.0	42.000004	1
ATAACGG	75	0.0	42.000004	1
CTTGCGG	610	0.0	41.68033	1
ATAGCGG	365	0.0	41.30137	1
CATACGA	235	0.0	41.17021	17
CGTTCAG	1180	0.0	40.99576	38
CGCACTT	3805	0.0	40.978973	34
CCCGCAC	3895	0.0	40.898586	32
CACTTAC	3815	0.0	40.871555	36
ACCCGCA	3900	0.0	40.788464	31
CCGCACT	3830	0.0	40.711487	33
AGGGCGA	1140	0.0	40.657894	5
>>END_MODULE