##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043272_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2193587
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30879696132408	33.0	31.0	34.0	31.0	34.0
2	32.655544092848835	34.0	31.0	34.0	31.0	34.0
3	32.78231362603808	34.0	31.0	34.0	31.0	34.0
4	36.21888897043974	37.0	37.0	37.0	35.0	37.0
5	36.16796871972709	37.0	37.0	37.0	35.0	37.0
6	36.310235244829585	37.0	37.0	37.0	35.0	37.0
7	36.24846108223654	37.0	37.0	37.0	35.0	37.0
8	36.23183762485828	37.0	37.0	37.0	35.0	37.0
9	37.83203355964454	39.0	38.0	39.0	35.0	39.0
10	37.647762318066256	39.0	37.0	39.0	35.0	39.0
11	37.279526638332555	39.0	37.0	39.0	35.0	39.0
12	37.284626504442265	39.0	37.0	39.0	35.0	39.0
13	37.275603839738295	39.0	37.0	39.0	35.0	39.0
14	38.56606553558168	40.0	37.0	41.0	35.0	41.0
15	38.62982731024573	40.0	38.0	41.0	35.0	41.0
16	38.51946606175183	40.0	38.0	41.0	34.0	41.0
17	38.488027144581	40.0	37.0	41.0	34.0	41.0
18	38.28512751032897	40.0	37.0	41.0	34.0	41.0
19	38.04780845254827	40.0	37.0	41.0	34.0	41.0
20	37.74546074534541	40.0	35.0	41.0	34.0	41.0
21	37.6974754135578	39.0	35.0	41.0	34.0	41.0
22	37.654569433535116	39.0	35.0	41.0	34.0	41.0
23	37.672366767308525	39.0	35.0	41.0	34.0	41.0
24	37.58403883684577	39.0	35.0	41.0	34.0	41.0
25	37.477116704283894	39.0	35.0	41.0	34.0	41.0
26	37.38071432771985	39.0	35.0	41.0	34.0	41.0
27	37.41938295586179	39.0	35.0	41.0	34.0	41.0
28	37.3800510305723	39.0	35.0	41.0	34.0	41.0
29	37.266526014240604	39.0	35.0	41.0	33.0	41.0
30	37.199507929250125	39.0	35.0	41.0	33.0	41.0
31	37.03665320773692	39.0	35.0	41.0	33.0	41.0
32	36.804235254858824	39.0	35.0	41.0	33.0	41.0
33	36.358514159684574	39.0	35.0	41.0	31.0	41.0
34	36.1388798347182	39.0	35.0	41.0	30.0	41.0
35	35.96803682735173	39.0	35.0	41.0	30.0	41.0
36	35.91279215276166	38.0	35.0	41.0	30.0	41.0
37	35.852954088440534	38.0	35.0	41.0	30.0	41.0
38	35.78763094420235	38.0	35.0	41.0	30.0	41.0
39	35.7892994442436	38.0	35.0	41.0	30.0	41.0
40	35.73367958508142	38.0	35.0	41.0	30.0	41.0
41	35.71352218991086	38.0	35.0	41.0	30.0	41.0
42	35.631216359323794	38.0	35.0	41.0	30.0	41.0
43	35.513808205464386	38.0	35.0	41.0	29.0	41.0
44	35.29937814182889	37.0	35.0	40.0	28.0	41.0
45	35.208232908017784	37.0	35.0	40.0	27.0	41.0
46	35.19151098178463	37.0	35.0	40.0	27.0	41.0
47	35.132466138794584	37.0	35.0	40.0	27.0	41.0
48	35.05007323621083	37.0	35.0	40.0	27.0	41.0
49	34.99973240176934	36.0	35.0	40.0	27.0	41.0
50	34.89705354745447	36.0	35.0	40.0	27.0	41.0
51	34.782072924392786	36.0	35.0	40.0	26.0	41.0
52	34.41010910440297	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	12.0
15	45.0
16	85.0
17	199.0
18	419.0
19	769.0
20	1514.0
21	2515.0
22	4065.0
23	6599.0
24	11564.0
25	20508.0
26	30454.0
27	36125.0
28	37391.0
29	37796.0
30	39672.0
31	44907.0
32	52775.0
33	68057.0
34	151944.0
35	222901.0
36	206913.0
37	197621.0
38	296533.0
39	720417.0
40	1777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.568413288371968	17.33147579740398	23.548644298129044	30.55146661609501
2	35.78540536573202	19.591928653844136	24.27859027246241	20.344075707961434
3	25.540313650655293	20.516168266861538	34.69695981969259	19.24655826279058
4	22.614512212189442	23.030178424653318	32.98068414883932	21.37462521431792
5	20.87416637680657	31.164344062943478	29.71407106260203	18.247418497647917
6	76.80598034178722	2.0566770317293095	18.141108604308833	2.996234022174639
7	76.69128236080903	1.9929002132124234	16.563965778425928	4.751851647552615
8	67.29785506569833	3.4498745661785923	22.059804329620842	7.192466038502234
9	41.03684968957238	15.14172904926953	26.404560202079974	17.416861059078123
10	28.81896181915739	15.506154987242358	35.74465020078985	19.930232992810407
11	25.619271084301648	12.891442190348501	39.81829761026118	21.670989115088666
12	21.292704597538187	14.268410598713432	41.931183946659054	22.507700857089326
13	16.91950216699862	17.632398441456846	43.577391733266104	21.870707658278427
14	17.306265947053845	17.62054570892333	43.474820009418366	21.598368334604462
15	21.098547721152613	14.753050597035813	42.933195720069456	21.215205961742114
16	19.906162828280802	18.803311653469866	39.038387809555765	22.25213770869357
17	20.563943896458177	20.073787818764423	38.970690471816255	20.391577812961145
18	21.007600792674282	25.76556115622494	35.012835141710816	18.21400290938996
19	22.504783261388766	21.12590018084535	33.923888133910346	22.44542842385554
20	21.243470170091268	25.47726623106355	34.69714216942387	18.58212142942131
21	19.100268190867286	20.194548928307835	36.27036447608415	24.434818404740728
22	20.754864065113445	21.617970930717586	34.09164988669244	23.535515117476535
23	21.38602207252322	19.634005854338124	37.07790026107923	21.902071812059425
24	23.56605869746675	18.02467830088344	33.91367654895839	24.495586452691416
25	17.982008463762778	19.448145890726014	36.82065037766909	25.749195267842122
26	17.324318570451048	19.579437697251123	36.563172557094845	26.533071175202988
27	21.517678578510903	21.328490732302843	35.695051073880364	21.45877961530589
28	22.678744905034538	21.83569650987173	32.46212710049795	23.023431484595775
29	21.42290230567559	20.79402366990687	34.96888885647116	22.814185167946384
30	26.85355082793616	19.34156247279	30.762627604922894	23.042259094350943
31	24.816066105424586	21.04748979639285	31.550788730968954	22.585655367213608
32	24.902089591158227	20.999896516527496	28.867056560783777	25.2309573315305
33	20.577027489677864	20.88784260665294	34.22029762211392	24.31483228155528
34	20.39171457525961	23.100747770660565	32.473843070733004	24.033694583346822
35	20.87147671827012	23.782553415934725	29.92263356775911	25.423336298036048
36	21.635750029517865	23.453731262995266	27.334452656767205	27.576066050719668
37	19.794975079629847	26.265746469139362	28.927687846435994	25.011590604794794
38	19.25143611810245	22.2592949356465	27.880726864263877	30.60854208198717
39	20.75117148305492	19.231332060228294	31.93705104926315	28.080445407453634
40	17.45975883336289	20.14713799817377	34.2885875964801	28.104515571983242
41	19.219479327694774	23.023932946356812	29.90403389516805	27.852553830780362
42	19.086455198722458	17.693987063198314	32.75137936174859	30.46817837633064
43	21.358076976203815	19.353779904786087	33.66837057294742	25.61977254606268
44	21.541247281279475	20.78130477614975	32.694668595318994	24.982779347251785
45	21.70176063224299	21.438949082028657	32.27079664494729	24.58849364078106
46	21.762209568163925	20.27510192210293	31.653223692518235	26.309464817214913
47	19.077839173919248	20.445416571123005	34.32710897721403	26.149635277743716
48	20.90589523005014	18.69444886389279	31.617802257216148	28.781853648840915
49	20.079759772464005	17.31857455391557	35.64567988413498	26.955985789485442
50	19.236711377301198	17.064014329041886	37.069193061410374	26.630081232246543
51	18.177988837461196	16.232545141815667	35.4534376799279	30.136028340795235
52	16.01135491776711	17.389736536549496	39.140230134478365	27.458678411205028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	787.0
1	3532.5
2	6278.0
3	76382.5
4	146487.0
5	82785.0
6	19083.0
7	17294.5
8	15506.0
9	15057.5
10	14609.0
11	14286.5
12	13964.0
13	13248.0
14	11708.0
15	10884.0
16	10204.5
17	9525.0
18	8761.5
19	7998.0
20	7530.0
21	7062.0
22	6868.5
23	6675.0
24	6707.0
25	6739.0
26	7657.0
27	8575.0
28	8883.0
29	9191.0
30	9853.5
31	10516.0
32	12612.0
33	14708.0
34	16869.0
35	19030.0
36	22117.0
37	25204.0
38	27751.0
39	32511.5
40	34725.0
41	40106.0
42	45487.0
43	53860.0
44	62233.0
45	72876.0
46	83519.0
47	104393.5
48	125268.0
49	164050.0
50	202832.0
51	221163.5
52	239495.0
53	222372.5
54	205250.0
55	185759.0
56	166268.0
57	146951.0
58	127634.0
59	118518.0
60	109402.0
61	105172.5
62	100943.0
63	100054.0
64	81855.0
65	64545.0
66	54148.0
67	43751.0
68	35408.0
69	27065.0
70	23877.0
71	20689.0
72	19547.5
73	18406.0
74	14913.0
75	11420.0
76	8174.0
77	4928.0
78	3832.0
79	2736.0
80	1986.0
81	1236.0
82	906.5
83	577.0
84	402.5
85	228.0
86	151.5
87	75.0
88	46.5
89	19.5
90	21.0
91	17.0
92	13.0
93	9.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2193587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.283020249803304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62436100017807	22.598510352970248
2	10.165852013576258	6.157054047672671
3	3.706651393665343	3.367457976399877
4	1.869784282095354	2.264908611098302
5	1.1329407706489212	1.7154434149694524
6	0.8597572307523951	1.5621627377273752
7	0.6975762383366532	1.4787300745931342
8	0.5778662442009264	1.399962813985155
9	0.5006456719848826	1.3644956720425128
>10	5.443983456690483	34.11796110606644
>50	0.27124924734816613	5.4780413392078735
>100	0.13784572244084878	7.2783915403828185
>500	0.005894505199858923	1.2312616693063085
>1k	0.004685375928092991	2.8458479912441303
>5k	4.534234769122249E-4	0.9529794140227744
>10k+	4.534234769122249E-4	6.186791238310957
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110038	5.01634993278133	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14087	0.6421901661525165	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11046	0.5035587829431885	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	8361	0.38115652581821463	RNA PCR Primer, Index 16 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	7192	0.3278648168502093	RNA PCR Primer, Index 16 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	5268	0.24015459610218332	RNA PCR Primer, Index 16 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	4803	0.21895643984031635	Illumina PCR Primer Index 7 (95% over 23bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4270	0.19465833814660644	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	4148	0.1890966713424177	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3843	0.1751925043319458	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	3449	0.1572310558003854	RNA PCR Primer, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	2876	0.1311094567938267	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	2599	0.11848173790234898	RNA PCR Primer, Index 16 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2532	0.11542737990332728	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	2213	0.10088498883335834	RNA PCR Primer, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34031018601040214	0.0
2	0.0	0.0	0.0	1.110190751495154	0.0
3	0.0	0.0	0.0	1.8596937345088205	0.0
4	0.0	0.0	0.0	2.3221782404800906	0.0
5	0.0	0.0	0.0	4.7258212234117	0.0
6	0.0	0.0	0.0	5.653525481323513	0.0
7	0.0	0.0	0.0	8.200814465074783	0.0
8	0.0	0.0	0.0	11.168054879975127	0.0
9	0.0	0.0	0.0	13.83797405801548	0.0
10	0.0	0.0	0.0	15.371033836360263	0.0
11	0.0	0.0	0.0	16.577049371645618	0.0
12	0.0	0.0	0.0	17.423471236837198	0.0
13	4.558743282121931E-5	0.0	0.0	17.878707340989894	0.0
14	4.558743282121931E-5	0.0	0.0	18.16112148731735	0.0
15	4.558743282121931E-5	0.0	0.0	18.881038226430043	0.0
16	4.558743282121931E-5	0.0	0.0	19.67849918877163	0.0
17	4.558743282121931E-5	0.0	0.0	20.692637219312477	0.0
18	4.558743282121931E-5	0.0	0.0	21.286185594644753	0.0
19	4.558743282121931E-5	0.0	0.0	21.916523028263754	0.0
20	9.117486564243862E-5	0.0	0.0	22.6502983469541	0.0
21	1.3676229846365794E-4	0.0	0.0	23.37787377478076	0.0
22	1.3676229846365794E-4	0.0	0.0	24.052658955400446	0.0
23	2.2793716410609655E-4	0.0	0.0	24.66694961266638	0.0
24	2.2793716410609655E-4	0.0	0.0	25.169368709789033	0.0
25	2.2793716410609655E-4	0.0	0.0	25.664584992525942	0.0
26	2.2793716410609655E-4	0.0	0.0	26.140198679149723	0.0
27	2.2793716410609655E-4	0.0	0.0	26.65159850053816	0.0
28	2.735245969273159E-4	0.0	0.0	27.13172534301124	0.0
29	2.735245969273159E-4	0.0	0.0	27.659673402513782	0.0
30	2.735245969273159E-4	0.0	0.0	28.14039288161354	0.0
31	3.1911202974853515E-4	0.0	0.0	28.62981956038215	0.0
32	3.1911202974853515E-4	0.0	0.0	29.174452620297256	0.0
33	3.1911202974853515E-4	0.0	0.0	29.696930187861252	0.0
34	3.1911202974853515E-4	0.0	0.0	30.28049491540568	0.0
35	3.1911202974853515E-4	0.0	0.0	30.783096362259624	0.0
36	3.1911202974853515E-4	0.0	0.0	31.310998834329343	0.0
37	3.1911202974853515E-4	0.0	0.0	32.008395381628354	0.0
38	3.1911202974853515E-4	0.0	0.0	32.69722149155698	0.0
39	3.1911202974853515E-4	0.0	0.0	33.44494656469062	0.0
40	3.646994625697545E-4	0.0	0.0	34.10204382137567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	6.312714E-4	46.0	31
AATGCGT	25	3.4181794E-5	46.0	26
CGTTTTT	26375	0.0	45.494217	1
CACTTAC	3610	0.0	42.750694	36
TACGGGA	325	0.0	42.461536	3
ACCCGCA	3775	0.0	41.856953	31
TTACGGG	660	0.0	41.818184	2
ATTGCGG	495	0.0	41.81818	1
ACTTACT	3620	0.0	41.80663	37
CGCACTT	3710	0.0	41.784367	34
ATAGCGG	325	0.0	41.753845	1
GCACTTA	3700	0.0	41.71081	35
CCGCACT	3740	0.0	41.572193	33
CACGACG	415	0.0	41.566265	25
CCCGCAC	3825	0.0	41.490192	32
GTCGGCG	1555	0.0	41.41479	3
CTCGAGG	285	0.0	41.157898	1
CTTACTG	3695	0.0	41.14479	38
TCAACGC	4415	0.0	40.998867	15
ATCAACG	4410	0.0	40.9932	14
>>END_MODULE