SRR1043271_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043271_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2143049
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103130	4.81230247185202	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13537	0.6316701111360497	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9058	0.42266882371798314	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	8182	0.3817924835129761	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	6956	0.3245842722214938	RNA PCR Primer, Index 16 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	5140	0.23984519252709574	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4777	0.22290670908597984	Illumina PCR Primer Index 7 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4199	0.19593579054888619	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	3909	0.18240366879152087	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3796	0.17713080755503025	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	3106	0.14493369027026448	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	2607	0.1216491083498324	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	2441	0.11390313520596124	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2390	0.11152334827621768	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	2221	0.10363738766589099	RNA PCR Primer, Index 16 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	2210	0.10312410028888747	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24785	44.228363	1
TACGCGG	160	43.59375	1
CGGTCTA	350	42.42857	30
ACCCGCA	4080	41.856617	31
CACGACG	355	41.830982	25
TACGGGA	270	41.666664	3
CACTTAC	4010	41.5212	36
ATTGCGG	425	41.294117	1
CGCACTT	4055	41.282368	34
TACGGGT	120	41.250004	3
ACTTACT	3995	41.057575	37
CGTTCAG	1125	41.0	38
CTTACTG	3985	40.99122	38
CCCGCAC	4190	40.918858	32
CCGCACT	4115	40.84447	33
AATGCGG	295	40.42373	1
TATGACC	4465	40.263157	27
TCGGCGT	1300	40.153847	4
CTTATGA	4560	40.11513	25
GCACTTA	4145	40.060314	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content