##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043271_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2143049
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.319331009230304	33.0	31.0	34.0	31.0	34.0
2	32.61409981759633	34.0	31.0	34.0	31.0	34.0
3	32.711993519513555	34.0	31.0	34.0	31.0	34.0
4	36.19859041953777	37.0	37.0	37.0	35.0	37.0
5	36.18921825865858	37.0	37.0	37.0	35.0	37.0
6	36.333461344094324	37.0	37.0	37.0	35.0	37.0
7	36.28309665341296	37.0	37.0	37.0	35.0	37.0
8	36.18828360900754	37.0	37.0	37.0	35.0	37.0
9	37.797612653747066	39.0	38.0	39.0	35.0	39.0
10	37.59823597127271	39.0	37.0	39.0	35.0	39.0
11	37.27760447847903	39.0	37.0	39.0	35.0	39.0
12	37.291830471445124	39.0	37.0	39.0	35.0	39.0
13	37.291550496512215	39.0	37.0	39.0	35.0	39.0
14	38.582055753274894	40.0	38.0	41.0	35.0	41.0
15	38.65339896567927	40.0	38.0	41.0	35.0	41.0
16	38.581436542048266	40.0	38.0	41.0	34.0	41.0
17	38.52627121451726	40.0	37.0	41.0	34.0	41.0
18	38.341037465778896	40.0	37.0	41.0	34.0	41.0
19	38.12911370668613	40.0	37.0	41.0	34.0	41.0
20	37.84472543558267	40.0	35.0	41.0	34.0	41.0
21	37.798530971527015	40.0	35.0	41.0	34.0	41.0
22	37.77692670582894	40.0	35.0	41.0	34.0	41.0
23	37.76046604627332	40.0	35.0	41.0	34.0	41.0
24	37.6695115230683	39.0	35.0	41.0	34.0	41.0
25	37.63239337971274	39.0	35.0	41.0	34.0	41.0
26	37.59808945105782	39.0	35.0	41.0	34.0	41.0
27	37.580863526685576	39.0	35.0	41.0	34.0	41.0
28	37.47023189857068	39.0	35.0	41.0	34.0	41.0
29	37.40302484917517	39.0	35.0	41.0	34.0	41.0
30	37.29054352000351	39.0	35.0	41.0	33.0	41.0
31	37.12136073416893	39.0	35.0	41.0	33.0	41.0
32	36.90065322818097	39.0	35.0	41.0	33.0	41.0
33	36.44085319561055	39.0	35.0	41.0	31.0	41.0
34	36.1846714657481	39.0	35.0	41.0	31.0	41.0
35	36.0051776697593	38.0	35.0	41.0	30.0	41.0
36	35.91542470564136	38.0	35.0	41.0	30.0	41.0
37	35.79805641401573	38.0	35.0	41.0	30.0	41.0
38	35.74118370601885	38.0	35.0	41.0	30.0	41.0
39	35.69837880515098	38.0	35.0	41.0	29.0	41.0
40	35.62323493303233	38.0	35.0	41.0	29.0	41.0
41	35.57205458204642	38.0	35.0	40.0	29.0	41.0
42	35.475062399413176	38.0	35.0	40.0	29.0	41.0
43	35.345169895788665	37.0	35.0	40.0	28.0	41.0
44	35.2141738243036	37.0	35.0	40.0	28.0	41.0
45	35.1394858447007	37.0	35.0	40.0	27.0	41.0
46	35.061665878848316	37.0	35.0	40.0	27.0	41.0
47	34.93954081311253	37.0	35.0	40.0	26.0	41.0
48	34.880866466422376	36.0	35.0	40.0	26.0	41.0
49	34.80929554107256	36.0	35.0	40.0	27.0	41.0
50	34.73149983971435	36.0	35.0	40.0	26.0	41.0
51	34.32886648882037	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	9.0
14	11.0
15	35.0
16	82.0
17	150.0
18	364.0
19	740.0
20	1341.0
21	2252.0
22	3507.0
23	5661.0
24	9718.0
25	17365.0
26	27966.0
27	35192.0
28	37492.0
29	37073.0
30	39273.0
31	44236.0
32	50790.0
33	65387.0
34	148061.0
35	222007.0
36	195301.0
37	194459.0
38	309224.0
39	695179.0
40	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.850903549102235	17.406648191431927	23.687419186402177	30.05502907306366
2	35.467084513699874	19.66478601282565	24.284279080879625	20.583850392594847
3	25.736369070422562	20.62351350809058	34.31046140335569	19.32965601813118
4	22.782913503144353	23.231759983089514	32.457353984906554	21.527972528859582
5	21.06018107845411	31.31127659703535	29.369230474898146	18.2593118496124
6	77.19179542791602	2.0412039108765128	17.759183294455703	3.0078173667517634
7	77.12427480659565	1.9782562134603547	16.136728558236417	4.760740421707577
8	67.72346315926514	3.410887945165976	21.658114210174382	7.207534685394501
9	41.272458072587234	15.134651610859107	26.004538393662486	17.58835192289117
10	29.187713393394176	15.472488029905056	35.36666683776246	19.973131738938307
11	25.89483488245019	12.887059511938364	39.49611044824453	21.721995157366912
12	21.519246643450522	14.186609825533619	41.73147697509483	22.562666555921027
13	17.060459186887467	17.5967511708785	43.35883127264006	21.983958369593974
14	17.42027363816693	17.539356309631746	43.358224660285416	21.68214539191591
15	21.28360107491709	14.726868121074228	42.69818375594772	21.291347048060963
16	19.982510899190824	18.690846546205897	38.91684231205166	22.40980024255162
17	20.76312767463553	19.9695853897881	38.78712059313622	20.48016634244014
18	21.16605826558329	25.851112130427257	34.853146148314856	18.129683455674602
19	22.678249540724455	21.137827459848094	33.612110595698	22.571812403729453
20	21.34248913580604	25.684060420457023	34.30070894319262	18.67274150054432
21	19.256629223130222	20.20602422063145	35.9912442505981	24.546102305640236
22	20.848053404285203	21.69399766407581	33.71308822150124	23.744860710137754
23	21.64943498725414	19.616070374499138	36.71955237607726	22.01494226216946
24	23.708323981392866	18.04657756308885	33.561108495419376	24.683989960098906
25	17.880739077827897	19.464743923260738	36.70909997858192	25.945417020329447
26	17.43123932303928	19.470390084407775	36.346019153085166	26.752351439467787
27	21.591386851163925	21.3991373972317	35.47142412515999	21.53805162644438
28	22.67894947805673	21.9426620669896	32.18834473686789	23.190043718085775
29	21.49484216179845	20.874371047978837	34.672375666631986	22.958411123590736
30	27.03269967228934	19.269321420088854	30.490016793829728	23.207962113792078
31	24.96653132989493	21.098584306751736	31.28281247885606	22.652071884497275
32	25.122617354992816	20.866111787457964	28.5426044854784	25.46866637207082
33	20.701393201928653	20.897142342522265	33.951253564430864	24.450210891118214
34	20.574284582387055	23.006333499607333	32.23766698754905	24.18171493045656
35	20.899615454429647	23.73445497513123	29.721998890366013	25.64393068007311
36	21.62559045546789	23.39493870648781	27.1274245245909	27.852046313453403
37	19.839677020917392	26.13808643666104	28.75020589823191	25.27203064418966
38	19.323403244629496	22.213351164625728	27.66567633311231	30.797569257632468
39	20.767980573472656	19.166243982288787	31.67827707159286	28.3874983726457
40	17.501932993599308	20.046625158827446	34.09833372918678	28.353108118386466
41	19.33058926790755	22.987388529147022	29.609542292313428	28.072479910632005
42	19.1932615633147	17.563947441239094	32.533040541770156	30.709750453676047
43	21.544911012300698	19.173709980499744	33.52349852943167	25.75788047776789
44	21.59679969986687	20.564018834846987	32.63859109147761	25.20059037380853
45	21.815040160071	21.281967887808445	32.16310033041708	24.73989162170347
46	21.920824022222543	20.13458395025032	31.507958987405328	26.43663304012181
47	19.316777171217268	20.21642995563797	34.194038493753524	26.272754379391234
48	21.064100727514866	18.578343285664488	31.372591107342856	28.984964879477793
49	20.24228097444342	17.177628696310723	35.45285245461023	27.127237874635625
50	19.427460594694754	16.93680359151844	36.93014018811516	26.705595625671645
51	18.208776374221962	16.183251059588464	35.157852200299665	30.45012036588991
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	614.0
1	3294.5
2	5975.0
3	71841.5
4	137708.0
5	78555.0
6	19402.0
7	17159.0
8	14916.0
9	14506.5
10	14097.0
11	13737.5
12	13378.0
13	12786.0
14	12194.0
15	11311.0
16	10428.0
17	9508.0
18	8588.0
19	7950.5
20	7313.0
21	6874.5
22	6436.0
23	6353.0
24	6270.0
25	6638.0
26	7562.0
27	8118.0
28	8412.5
29	8707.0
30	10175.0
31	11643.0
32	13158.5
33	14674.0
34	17629.0
35	20584.0
36	23522.0
37	26460.0
38	28918.0
39	31376.0
40	35069.5
41	38763.0
42	45423.5
43	52084.0
44	59368.0
45	66652.0
46	79092.5
47	91533.0
48	124245.5
49	156958.0
50	191728.0
51	226498.0
52	232579.0
53	238660.0
54	213171.5
55	187683.0
56	166074.5
57	144466.0
58	129739.0
59	115012.0
60	109599.0
61	104186.0
62	100378.5
63	96571.0
64	91333.0
65	86095.0
66	69308.0
67	52521.0
68	42278.0
69	32035.0
70	27426.5
71	22818.0
72	20826.0
73	18834.0
74	16152.5
75	9927.0
76	6383.0
77	4726.0
78	3069.0
79	2365.0
80	1661.0
81	1190.0
82	719.0
83	493.0
84	267.0
85	214.0
86	161.0
87	93.0
88	25.0
89	24.5
90	24.0
91	16.5
92	9.0
93	5.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2143049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.98235110373321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9057558666944	22.158683209820218
2	10.389333385551987	6.22993282598714
3	3.790545975554533	3.4094844094175683
4	1.9914875104195824	2.388379110243979
5	1.1920427059296916	1.787012146991411
6	0.8586257342492974	1.54461709385779
7	0.7120537769517137	1.494433244171393
8	0.5855457140192585	1.4044829748009253
9	0.5312924618655628	1.4336457417378081
>10	5.623944448814864	34.50343246494806
>50	0.2680078886244845	5.418376723053037
>100	0.13914285533682372	7.253133219309044
>500	0.0064242143012600075	1.286608186830914
>1k	0.004857332764367323	2.8287340254007316
>5k	6.26752614757074E-4	1.3781698531738307
>10k+	3.13376307378537E-4	5.480874770256044
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103130	4.81230247185202	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13537	0.6316701111360497	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9058	0.42266882371798314	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	8182	0.3817924835129761	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	6956	0.3245842722214938	RNA PCR Primer, Index 16 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	5140	0.23984519252709574	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4777	0.22290670908597984	Illumina PCR Primer Index 7 (95% over 22bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4199	0.19593579054888619	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	3909	0.18240366879152087	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3796	0.17713080755503025	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	3106	0.14493369027026448	RNA PCR Primer, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	2607	0.1216491083498324	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	2441	0.11390313520596124	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2390	0.11152334827621768	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	2221	0.10363738766589099	RNA PCR Primer, Index 16 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	2210	0.10312410028888747	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33587659451557106	0.0
2	0.0	0.0	0.0	1.107907472017672	0.0
3	0.0	0.0	0.0	1.8558605052894264	0.0
4	0.0	0.0	0.0	2.326125067602281	0.0
5	0.0	0.0	0.0	4.7634468460590496	0.0
6	0.0	0.0	0.0	5.688250711952923	0.0
7	0.0	0.0	0.0	8.278392141290283	0.0
8	0.0	0.0	0.0	11.3019814292627	0.0
9	0.0	0.0	0.0	14.067480491579987	0.0
10	0.0	0.0	0.0	15.633426953840067	0.0
11	0.0	0.0	0.0	16.869656270108617	0.0
12	0.0	0.0	0.0	17.739678374129568	0.0
13	0.0	0.0	0.0	18.210502886308245	0.0
14	0.0	0.0	0.0	18.516142188069427	0.0
15	0.0	0.0	0.0	19.268108195379575	0.0
16	0.0	0.0	0.0	20.092774360268944	0.0
17	0.0	0.0	0.0	21.125321912844736	0.0
18	0.0	0.0	0.0	21.73450070437027	4.666248881850112E-5
19	0.0	0.0	0.0	22.38539576089954	4.666248881850112E-5
20	0.0	0.0	0.0	23.15532682640481	4.666248881850112E-5
21	0.0	0.0	0.0	23.90990593308879	4.666248881850112E-5
22	0.0	0.0	0.0	24.621648875037387	4.666248881850112E-5
23	0.0	0.0	0.0	25.236613815176415	4.666248881850112E-5
24	4.666248881850112E-5	0.0	0.0	25.76254672664974	4.666248881850112E-5
25	4.666248881850112E-5	0.0	0.0	26.27774726569481	4.666248881850112E-5
26	4.666248881850112E-5	0.0	0.0	26.7594441377682	4.666248881850112E-5
27	4.666248881850112E-5	0.0	0.0	27.290043298123376	4.666248881850112E-5
28	9.332497763700224E-5	0.0	0.0	27.792504977720995	4.666248881850112E-5
29	9.332497763700224E-5	0.0	0.0	28.331036761175316	4.666248881850112E-5
30	9.332497763700224E-5	0.0	0.0	28.82873886691345	4.666248881850112E-5
31	9.332497763700224E-5	0.0	0.0	29.329474034424784	4.666248881850112E-5
32	9.332497763700224E-5	0.0	0.0	29.8911970748219	4.666248881850112E-5
33	9.332497763700224E-5	0.0	0.0	30.428982258455125	4.666248881850112E-5
34	9.332497763700224E-5	0.0	0.0	31.027895302440587	4.666248881850112E-5
35	9.332497763700224E-5	0.0	0.0	31.53842959260381	4.666248881850112E-5
36	9.332497763700224E-5	0.0	0.0	32.07556150139357	4.666248881850112E-5
37	9.332497763700224E-5	0.0	0.0	32.78142496975104	4.666248881850112E-5
38	9.332497763700224E-5	0.0	0.0	33.490601474814625	4.666248881850112E-5
39	9.332497763700224E-5	0.0	0.0	34.263518939604275	4.666248881850112E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24785	0.0	44.228363	1
TACGCGG	160	0.0	43.59375	1
CGGTCTA	350	0.0	42.42857	30
ACCCGCA	4080	0.0	41.856617	31
CACGACG	355	0.0	41.830982	25
TACGGGA	270	0.0	41.666664	3
CACTTAC	4010	0.0	41.5212	36
ATTGCGG	425	0.0	41.294117	1
CGCACTT	4055	0.0	41.282368	34
TACGGGT	120	0.0	41.250004	3
ACTTACT	3995	0.0	41.057575	37
CGTTCAG	1125	0.0	41.0	38
CTTACTG	3985	0.0	40.99122	38
CCCGCAC	4190	0.0	40.918858	32
CCGCACT	4115	0.0	40.84447	33
AATGCGG	295	0.0	40.42373	1
TATGACC	4465	0.0	40.263157	27
TCGGCGT	1300	0.0	40.153847	4
CTTATGA	4560	0.0	40.11513	25
GCACTTA	4145	0.0	40.060314	35
>>END_MODULE