FastQCFastQC Report
Thu 26 May 2016
SRR1043270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043270_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1039441
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724096.966148150784893No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT92590.8907672489347639Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT87060.8375655761125452Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT63460.6105204624408697Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC39750.3824170876461483No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG34540.33229399263642667No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT32860.3161314591208159Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT21340.2053026578709133No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC21000.2020316689451349No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC20880.20087720226544845No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC17460.16797490189438363No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT16600.15970122402329714Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC16140.15527576841783228No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT14160.13622706820300529No Hit
TGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC13890.1336295181737107No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC13300.1279533903319188No Hit
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC12950.12458619584949987No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC12660.1217962347069242No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT12640.12160382359364312No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTG12520.12044935691395663Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG12480.12006453468739448No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGT10820.10409441228506476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCA551.8189894E-1246.0000044
TCACCGG253.416929E-546.01
TACCAGG1200.046.01
ACTCTGC206.311175E-446.028
GCAGTAA206.311175E-446.016
GTTAGCG206.311175E-446.08
CGATTGA253.416929E-546.09
CCTCGCG206.311175E-446.026
ACCTCGG301.86123E-646.01
GCACCGC253.416929E-546.08
GACCACT206.311175E-446.011
GTTACGG1500.046.01
ATTCCCG206.311175E-446.010
GTAGCTT206.311175E-446.02
GCTCTAA206.311175E-446.018
AAACGAT206.311175E-446.038
GAGACGA206.311175E-446.08
CACTCCA206.311175E-446.023
TCGCTTC253.416929E-546.013
TACTGAG206.311175E-446.017
Produced by FastQC (version 0.11.5)