##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043270_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1039441
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.142437136884155	33.0	31.0	34.0	30.0	34.0
2	32.463518371894125	34.0	31.0	34.0	31.0	34.0
3	32.5267100297179	34.0	31.0	34.0	31.0	34.0
4	36.08202870581399	37.0	35.0	37.0	35.0	37.0
5	35.95642369311967	37.0	35.0	37.0	35.0	37.0
6	36.237424731177626	37.0	36.0	37.0	35.0	37.0
7	36.158791119457476	37.0	37.0	37.0	35.0	37.0
8	36.13078856808612	37.0	36.0	37.0	35.0	37.0
9	37.671891911133	39.0	37.0	39.0	35.0	39.0
10	37.52493888542014	39.0	37.0	39.0	35.0	39.0
11	37.225262424707125	39.0	37.0	39.0	34.0	39.0
12	37.211462699662604	39.0	37.0	39.0	34.0	39.0
13	37.2121159353922	39.0	37.0	39.0	34.0	39.0
14	38.60245074034986	40.0	38.0	41.0	34.0	41.0
15	38.702644017313155	40.0	38.0	41.0	35.0	41.0
16	38.73253027348353	40.0	38.0	41.0	35.0	41.0
17	38.7017377609696	40.0	38.0	41.0	35.0	41.0
18	38.425026528682245	40.0	38.0	41.0	34.0	41.0
19	38.14990461219059	40.0	37.0	41.0	34.0	41.0
20	37.83063781397886	40.0	35.0	41.0	34.0	41.0
21	37.7795536254583	40.0	35.0	41.0	34.0	41.0
22	37.72555056034926	39.0	35.0	41.0	34.0	41.0
23	37.736798914031674	39.0	35.0	41.0	34.0	41.0
24	37.63738297796604	39.0	35.0	41.0	34.0	41.0
25	37.546245530049326	39.0	35.0	41.0	33.0	41.0
26	37.521188792822294	39.0	35.0	41.0	34.0	41.0
27	37.55728992795166	39.0	35.0	41.0	34.0	41.0
28	37.524839793696806	39.0	35.0	41.0	33.0	41.0
29	37.50486174780483	40.0	35.0	41.0	33.0	41.0
30	37.42800409066027	40.0	35.0	41.0	33.0	41.0
31	37.2879615100809	40.0	35.0	41.0	33.0	41.0
32	37.077611908708626	40.0	35.0	41.0	33.0	41.0
33	36.75023305796096	39.0	35.0	41.0	32.0	41.0
34	36.59937601075963	39.0	35.0	41.0	31.0	41.0
35	36.39852093577221	39.0	35.0	41.0	31.0	41.0
36	36.10132850253165	39.0	35.0	41.0	30.0	41.0
37	36.16314826911773	39.0	35.0	41.0	30.0	41.0
38	35.98033847038937	38.0	35.0	41.0	30.0	41.0
39	35.84422107652094	38.0	35.0	41.0	30.0	41.0
40	35.82419973812848	38.0	35.0	40.0	30.0	41.0
41	35.925429148936786	38.0	35.0	40.0	30.0	41.0
42	35.90031468837577	39.0	35.0	41.0	29.0	41.0
43	35.80937831007243	38.0	35.0	40.0	29.0	41.0
44	35.716003120908255	38.0	35.0	40.0	28.0	41.0
45	35.6715311402956	38.0	35.0	40.0	28.0	41.0
46	35.628800480258136	38.0	35.0	41.0	28.0	41.0
47	35.48412175390426	38.0	35.0	40.0	28.0	41.0
48	35.40855132710755	38.0	35.0	40.0	28.0	41.0
49	35.35912860855017	38.0	35.0	40.0	28.0	41.0
50	35.175457770089885	37.0	35.0	40.0	27.0	41.0
51	35.03786843120485	37.0	35.0	40.0	27.0	41.0
52	34.65903019026573	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	26.0
16	31.0
17	64.0
18	219.0
19	372.0
20	706.0
21	1165.0
22	1871.0
23	3145.0
24	5569.0
25	10394.0
26	14326.0
27	15735.0
28	15154.0
29	15286.0
30	17199.0
31	20493.0
32	25426.0
33	32899.0
34	56305.0
35	99371.0
36	113190.0
37	83976.0
38	143735.0
39	362545.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.524160582466923	19.12191264343046	26.03081848801423	28.323108286088388
2	33.878786770966315	19.616216793449556	27.18980682886282	19.315189606721304
3	20.45493683624179	21.805566645918333	38.75669710931164	18.982799408528237
4	22.391169869189305	23.906118769607897	36.584087023698316	17.118624337504485
5	19.536077564767986	28.54784446640069	32.6544748571588	19.261603111672525
6	70.33588245989912	1.5419826618345822	23.40123200835834	4.72090286990796
7	70.99642981179308	1.3775673655358986	19.265355128381508	8.360647694289526
8	57.42740569209796	3.0228747952024215	28.70061889034587	10.849100622353745
9	32.41020894884847	17.13748062660603	31.32501026994317	19.12730015460233
10	25.16381401156968	18.638960749094945	43.677226509248726	12.519998730086652
11	21.757848689824627	15.340841856343939	41.77062478774649	21.130684666084946
12	16.96296374686009	19.242265794787773	45.2365261712786	18.558244287073535
13	12.190590904149442	22.551544532109087	43.972962390361744	21.28490217337973
14	10.949154401259909	22.326231118456942	45.06893609161078	21.65567838867237
15	10.869592405918182	18.388152862933058	49.60858769280796	21.1336670383408
16	11.856565211493486	25.417700475544063	39.78744344315839	22.93829086980406
17	13.604908792322027	22.45091351986308	41.851918483107745	22.092259204707148
18	15.313038450474822	29.072549572318195	39.93810134485747	15.676310632349502
19	14.62901694276058	23.624909927547595	37.05299290676431	24.693080222927517
20	12.548283163738972	30.66099951801016	35.621165607283146	21.16955171096772
21	13.124169625789248	24.587254110622922	36.66057044122755	25.62800582236029
22	11.483095240614908	27.234831029370593	32.59723255095768	28.684841179056818
23	11.164847259247999	23.69869958949089	42.822824960724084	22.31362819053703
24	16.829815256469583	21.519258909356086	38.90090923871581	22.75001659545852
25	12.722319015701707	25.045577382458458	38.7093639754445	23.522739626395342
26	16.470487502417164	20.865542151983615	37.10273117954747	25.561239166051752
27	21.899848091426062	21.250749200772336	37.883727888355374	18.96567481944622
28	16.22602918299355	21.52195266494202	34.69028064122928	27.56173751083515
29	24.000400215115626	21.20149195577238	31.99113754412227	22.80697028498972
30	22.932807153075547	21.083736354444362	28.96374108775775	27.019715404722344
31	25.32207215224337	19.18617795526634	33.095769745468964	22.395980147021334
32	28.038243632875748	18.805973595422927	28.815488324974677	24.340294446726656
33	19.74715255603733	21.40958457478587	34.504892533582954	24.338370335593844
34	25.858899158297582	16.004275374937105	36.397544449372305	21.739281017393004
35	28.76363352994542	20.12427833806825	33.096346978808796	18.015741153177526
36	25.46493740385457	22.224253228417968	35.02526838945164	17.285540978275822
37	26.93313040374586	21.558703187578708	35.87928511574972	15.628881292925717
38	25.53699536577834	19.778900389728708	34.13671386831961	20.547390376173347
39	28.71841691832437	17.823330039896447	37.95030213355063	15.507950908228556
40	20.155641349533067	16.35734976780789	41.705686037014125	21.78132284564492
41	23.213727378465926	19.36954574622321	33.14675869048844	24.269968184822417
42	22.20895654491212	15.06300020876606	39.480162895248505	23.247880351073317
43	25.172857333893887	19.55454903164297	36.17800336911859	19.094590265344547
44	19.052452231535987	22.479775186855242	35.79683695370877	22.670935627899997
45	22.242628489736312	19.94600944161333	34.922713266072826	22.88864880257754
46	27.39443604783725	16.15406742662643	34.645256440721504	21.806240084814817
47	19.593319870969108	15.480917146812564	39.6576621472503	25.268100834968028
48	20.16506949408384	14.18897272668675	34.3915623878604	31.254395391369016
49	22.641881549794554	13.816945839157778	40.12233498582411	23.418837625223556
50	20.791463873370397	13.638388326032935	40.25788861513063	25.312259185466036
51	17.688834671712968	12.943206973748389	39.033480495766476	30.334477858772168
52	16.001677824907812	13.081454358640846	48.57562863115848	22.341239185292864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	573.0
1	1915.0
2	3257.0
3	40827.0
4	78397.0
5	42572.5
6	6748.0
7	6397.0
8	6046.0
9	6483.5
10	6921.0
11	7130.5
12	7340.0
13	7250.5
14	6611.5
15	6062.0
16	5513.0
17	4964.0
18	4510.0
19	4056.0
20	3737.5
21	3419.0
22	3273.0
23	3127.0
24	3029.0
25	2931.0
26	3127.5
27	3324.0
28	3879.5
29	4435.0
30	4524.5
31	4614.0
32	5222.5
33	5831.0
34	7361.5
35	8892.0
36	10700.5
37	12509.0
38	13803.0
39	16759.5
40	18422.0
41	23345.5
42	28269.0
43	35851.0
44	43433.0
45	54344.0
46	65255.0
47	83248.0
48	101241.0
49	130832.0
50	160423.0
51	165773.0
52	171123.0
53	144289.5
54	117456.0
55	90338.0
56	63220.0
57	47443.0
58	31666.0
59	24041.5
60	16417.0
61	13053.0
62	9689.0
63	7634.5
64	4442.5
65	3305.0
66	2774.0
67	2243.0
68	1928.5
69	1614.0
70	1535.5
71	1457.0
72	1366.0
73	1275.0
74	1037.5
75	800.0
76	608.5
77	417.0
78	313.0
79	209.0
80	153.5
81	98.0
82	94.0
83	90.0
84	52.5
85	15.0
86	11.0
87	7.0
88	6.0
89	4.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1039441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.9505818029862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3152685382996	21.95834947764243
2	11.822110652849876	7.081581843842696
3	4.737768810493878	4.256967969670005
4	2.444028317854042	2.92800280250809
5	1.3836102989181767	2.071996672060152
6	0.9325605217544324	1.6758438115824972
7	0.6535363574508625	1.3701655894540379
8	0.4684436929757981	1.1224128917251674
9	0.3920746094838891	1.056857639779898
>10	3.2313007819352473	20.681794303390095
>50	0.37041792967765297	7.561457490291589
>100	0.22985890565098693	13.19846250834306
>500	0.011605779247451847	2.3544095879334495
>1k	0.006125272380599586	3.3428483572770653
>5k	9.671482706209872E-4	2.3473610356073693
>10k+	3.223827568736624E-4	6.991488018892436
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72409	6.966148150784893	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	9259	0.8907672489347639	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	8706	0.8375655761125452	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	6346	0.6105204624408697	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3975	0.3824170876461483	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	3454	0.33229399263642667	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	3286	0.3161314591208159	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	2134	0.2053026578709133	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2100	0.2020316689451349	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2088	0.20087720226544845	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1746	0.16797490189438363	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1660	0.15970122402329714	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1614	0.15527576841783228	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	1416	0.13622706820300529	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1389	0.1336295181737107	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	1330	0.1279533903319188	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1295	0.12458619584949987	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1266	0.1217962347069242	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1264	0.12160382359364312	No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTTG	1252	0.12044935691395663	Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1248	0.12006453468739448	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGT	1082	0.10409441228506476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8693134098039235	0.0
2	0.0	0.0	0.0	2.9185879718040755	0.0
3	0.0	0.0	0.0	4.744954259068095	0.0
4	0.0	0.0	0.0	5.91712276117644	0.0
5	0.0	0.0	0.0	11.775079105018948	0.0
6	0.0	0.0	0.0	13.371417906355436	0.0
7	0.0	0.0	0.0	18.455496752581436	0.0
8	0.0	0.0	0.0	24.671337767126754	0.0
9	0.0	0.0	0.0	29.374635020169496	0.0
10	0.0	0.0	0.0	32.16440375163189	0.0
11	0.0	0.0	0.0	34.61071864588755	0.0
12	0.0	0.0	0.0	36.32500545966534	0.0
13	0.0	0.0	0.0	36.987188306022176	0.0
14	0.0	0.0	0.0	37.468600911451446	0.0
15	0.0	0.0	0.0	39.01500902889149	0.0
16	0.0	0.0	0.0	40.74719007620442	0.0
17	0.0	0.0	0.0	42.79001886590966	0.0
18	0.0	0.0	0.0	43.76669767692442	0.0
19	0.0	0.0	0.0	44.892302689618745	0.0
20	0.0	0.0	0.0	46.0583140361021	0.0
21	0.0	0.0	0.0	47.156115642927304	0.0
22	0.0	0.0	0.0	48.213126093736925	0.0
23	0.0	0.0	0.0	48.98382880798429	0.0
24	2.886166699216213E-4	0.0	0.0	49.65871078781768	0.0
25	2.886166699216213E-4	0.0	0.0	50.277408722573	0.0
26	3.8482222656216177E-4	0.0	0.0	50.922755596517746	0.0
27	3.8482222656216177E-4	0.0	0.0	51.54078009237657	0.0
28	3.8482222656216177E-4	0.0	0.0	52.14937644368464	0.0
29	3.8482222656216177E-4	0.0	0.0	52.90170389661366	0.0
30	3.8482222656216177E-4	0.0	0.0	53.541470848273256	0.0
31	3.8482222656216177E-4	0.0	0.0	54.09311351005012	0.0
32	3.8482222656216177E-4	0.0	0.0	54.63696352173909	0.0
33	3.8482222656216177E-4	0.0	0.0	55.17677290004916	0.0
34	3.8482222656216177E-4	0.0	0.0	55.77517146235332	0.0
35	3.8482222656216177E-4	0.0	0.0	56.295643523778644	0.0
36	4.810277832027022E-4	0.0	0.0	56.77003312357315	0.0
37	4.810277832027022E-4	0.0	0.0	57.262028340232874	0.0
38	4.810277832027022E-4	0.0	0.0	57.74363335677542	0.0
39	4.810277832027022E-4	0.0	0.0	58.20782516756603	0.0
40	4.810277832027022E-4	0.0	0.0	58.68336923404022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGCA	55	1.8189894E-12	46.000004	4
TCACCGG	25	3.416929E-5	46.0	1
TACCAGG	120	0.0	46.0	1
ACTCTGC	20	6.311175E-4	46.0	28
GCAGTAA	20	6.311175E-4	46.0	16
GTTAGCG	20	6.311175E-4	46.0	8
CGATTGA	25	3.416929E-5	46.0	9
CCTCGCG	20	6.311175E-4	46.0	26
ACCTCGG	30	1.86123E-6	46.0	1
GCACCGC	25	3.416929E-5	46.0	8
GACCACT	20	6.311175E-4	46.0	11
GTTACGG	150	0.0	46.0	1
ATTCCCG	20	6.311175E-4	46.0	10
GTAGCTT	20	6.311175E-4	46.0	2
GCTCTAA	20	6.311175E-4	46.0	18
AAACGAT	20	6.311175E-4	46.0	38
GAGACGA	20	6.311175E-4	46.0	8
CACTCCA	20	6.311175E-4	46.0	23
TCGCTTC	25	3.416929E-5	46.0	13
TACTGAG	20	6.311175E-4	46.0	17
>>END_MODULE