SRR1043269_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043269_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	997340
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65017	6.51904064812401	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	8489	0.8511640964966812	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	8383	0.8405358252952855	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	6297	0.6313794693885736	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	3804	0.3814145627368801	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3260	0.3268694727976417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	3152	0.31604066817735177	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	2015	0.20203741953596566	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1987	0.19922995167144605	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1872	0.18769928008502615	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1716	0.17205767341127398	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1666	0.16704433793891751	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1609	0.16132913550043115	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1407	0.141075260192111	No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	1332	0.13355525698357631	Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1308	0.13114885595684522	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	1233	0.12362885274831051	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	1198	0.12011951791766097	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1183	0.11861551727595403	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	1162	0.11650991637756432	No Hit
GTAATGGGTTTACCTGGCTTCCTTCTTGGTACATAGGCTACTCTTCCACAC	1084	0.10868911304068823	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1071	0.10738564581787556	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1035	0.10377604427777888	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	1029	0.10317444402109613	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	20	7.032836E-4	45.000004	44
CGGGTCC	40	6.8139343E-9	45.000004	5
AGTCCAC	20	7.032836E-4	45.000004	33
CAACCGG	35	1.2117198E-7	45.000004	1
ATTAACG	20	7.032836E-4	45.000004	9
TGCGGAT	20	7.032836E-4	45.000004	16
TGTGCGT	40	6.8139343E-9	45.000004	19
TAGCATA	20	7.032836E-4	45.000004	29
CACTCGC	35	1.2117198E-7	45.000004	21
TGTTCGG	20	7.032836E-4	45.000004	1
GGACGTA	20	7.032836E-4	45.000004	19
CATACCG	40	6.8139343E-9	45.000004	13
TCATCGG	45	3.8380676E-10	45.000004	1
CCGACAT	20	7.032836E-4	45.000004	9
GAGCATT	20	7.032836E-4	45.000004	8
GTCCACC	20	7.032836E-4	45.000004	34
CGATCTT	20	7.032836E-4	45.000004	9
TCGACCC	20	7.032836E-4	45.000004	23
TAGCTAA	20	7.032836E-4	45.000004	38
TAGTCGG	35	1.2117198E-7	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content