##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043269_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	997340
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06676760182084	33.0	31.0	34.0	30.0	34.0
2	32.36276194677843	34.0	31.0	34.0	30.0	34.0
3	32.41909880281549	34.0	31.0	34.0	30.0	34.0
4	36.025047626686984	37.0	35.0	37.0	35.0	37.0
5	35.96670844446227	37.0	35.0	37.0	35.0	37.0
6	36.23864579782221	37.0	36.0	37.0	35.0	37.0
7	36.17017767260914	37.0	37.0	37.0	35.0	37.0
8	36.063036677562316	37.0	36.0	37.0	35.0	37.0
9	37.61443439549201	39.0	37.0	39.0	35.0	39.0
10	37.4671566366535	39.0	37.0	39.0	35.0	39.0
11	37.21347584574969	39.0	37.0	39.0	34.0	39.0
12	37.208107566125896	39.0	37.0	39.0	34.0	39.0
13	37.20936290532817	39.0	37.0	39.0	34.0	39.0
14	38.606065133254454	40.0	38.0	41.0	34.0	41.0
15	38.72310846852628	40.0	38.0	41.0	35.0	41.0
16	38.77735476367137	40.0	38.0	41.0	35.0	41.0
17	38.72248180159224	40.0	38.0	41.0	35.0	41.0
18	38.4858724206389	40.0	38.0	41.0	35.0	41.0
19	38.23341588625745	40.0	37.0	41.0	34.0	41.0
20	37.94984959993583	40.0	35.0	41.0	34.0	41.0
21	37.89399201876993	40.0	35.0	41.0	34.0	41.0
22	37.859999598933165	40.0	35.0	41.0	34.0	41.0
23	37.83214751238294	40.0	35.0	41.0	34.0	41.0
24	37.74549702207873	40.0	35.0	41.0	34.0	41.0
25	37.71773517556701	39.0	35.0	41.0	34.0	41.0
26	37.73444462269637	40.0	35.0	41.0	34.0	41.0
27	37.73754286401829	40.0	35.0	41.0	34.0	41.0
28	37.659701806806105	40.0	35.0	41.0	34.0	41.0
29	37.671823049311165	40.0	35.0	41.0	34.0	41.0
30	37.53516052700183	40.0	35.0	41.0	33.0	41.0
31	37.387164858523676	40.0	35.0	41.0	33.0	41.0
32	37.200247658772334	40.0	35.0	41.0	33.0	41.0
33	36.85463532997774	39.0	35.0	41.0	32.0	41.0
34	36.65605310124932	39.0	35.0	41.0	31.0	41.0
35	36.463022640222995	39.0	35.0	41.0	31.0	41.0
36	36.1793260071791	39.0	35.0	41.0	30.0	41.0
37	36.16745242344637	39.0	35.0	41.0	30.0	41.0
38	36.003436140132756	38.0	35.0	41.0	30.0	41.0
39	35.82294503378988	38.0	35.0	40.0	30.0	41.0
40	35.7710560089839	38.0	35.0	40.0	30.0	41.0
41	35.821042974311666	38.0	35.0	40.0	29.0	41.0
42	35.78307096877695	38.0	35.0	40.0	28.0	41.0
43	35.672751519040645	38.0	35.0	40.0	28.0	41.0
44	35.604015681713356	38.0	35.0	40.0	28.0	41.0
45	35.583127118134236	38.0	35.0	40.0	28.0	41.0
46	35.48918222471775	38.0	35.0	40.0	28.0	41.0
47	35.29061303066156	38.0	35.0	40.0	26.0	41.0
48	35.243281127799946	38.0	35.0	40.0	26.0	41.0
49	35.18478954017687	38.0	35.0	40.0	27.0	41.0
50	35.03419495858985	37.0	35.0	40.0	26.0	41.0
51	34.60608217859506	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	2.0
14	1.0
15	16.0
16	30.0
17	62.0
18	141.0
19	304.0
20	595.0
21	1016.0
22	1568.0
23	2498.0
24	4569.0
25	8539.0
26	12876.0
27	15424.0
28	15015.0
29	15224.0
30	16547.0
31	19664.0
32	23961.0
33	31761.0
34	54979.0
35	95994.0
36	103646.0
37	82594.0
38	148222.0
39	342065.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.999618986504103	19.1739025808651	26.199189844987668	27.62728858764313
2	33.424208394328915	19.775202037419536	27.200954539073933	19.599635029177612
3	20.642108007299417	22.03391020113502	38.09312771973449	19.23085407183107
4	22.708003288748067	24.045861992901116	36.01259349870656	17.233541219644255
5	19.74371829065314	28.861672047646742	31.99741311889626	19.397196542803858
6	71.06302765355846	1.5229510497924479	22.74851103936471	4.665510257284376
7	71.79046263059739	1.3329456353901377	18.49038442256402	8.386207311448453
8	57.96659113241221	2.9735095353640686	28.082900515370884	10.976998816852829
9	32.765556379970725	17.25890869713438	30.653438145466943	19.32209677742796
10	25.61162692762749	18.69192050855275	43.154892012753926	12.541560551065837
11	22.015561393306193	15.398159103214551	41.38237712314757	21.203902380331684
12	17.367998877012855	19.237571941364028	44.7274750837227	18.666954097900415
13	12.304830850061164	22.613652315158323	43.59716846812521	21.484348366655304
14	11.046283113080795	22.33601379669922	44.69318386909178	21.9245192211282
15	11.05420418312712	18.269396594942545	49.36049892714621	21.315900294784125
16	12.006537389455954	25.40136763791686	39.40672188020133	23.185373092425856
17	13.85365071089097	22.24547295806846	41.54410732548579	22.356769005554774
18	15.576032245773758	29.09579481420579	39.59772996169812	15.730442978322337
19	14.812200453205527	23.57521005875629	36.703431126797284	24.9091583612409
20	12.714520624862132	30.69544989672529	35.14047365993543	21.449555818477148
21	13.232197645737662	24.64826438325947	36.23217759239577	25.887360378607095
22	11.65630577335713	27.260312431066637	32.09868249543786	28.984699300138367
23	11.326428299276074	23.702849579882486	42.39507088856358	22.57565123227786
24	16.94647763049712	21.554835863396633	38.50923456394008	22.989451942166163
25	12.742795836926224	25.05274028916919	38.380492109009964	23.82397176489462
26	16.57308440451601	20.748390719313374	36.776926624822025	25.901598251348588
27	22.178093729319993	21.126697014057395	37.5672288286843	19.127980427938315
28	16.36011791365031	21.53728918924339	34.29933623438346	27.80325666272284
29	24.182926584715343	21.143140754406723	31.697615657649347	22.976317003228587
30	23.185072292297512	20.977600417109514	28.49670122525919	27.34062606533379
31	25.58154691479335	19.103314817414322	32.64834459662703	22.6667936711653
32	28.421400926464397	18.631359416046685	28.219263240218982	24.727976417269936
33	19.966711452463553	21.29694988669862	34.048869994184535	24.6874686666533
34	26.16068742855997	15.773156596546814	36.050293781458684	22.015862193434536
35	29.01878998135039	19.959793049511703	32.79884492750717	18.222572041630738
36	25.70768243527784	22.078428620129547	34.678143862674716	17.535745081917902
37	27.385244751037764	21.340365371889224	35.47696873683999	15.79742114023302
38	25.917741191569576	19.603244630717708	33.69492850983617	20.78408566787655
39	29.097900415104178	17.628190988028155	37.673912607536046	15.599995989331623
40	20.35835321956404	16.078167926684984	41.53097238654822	22.032506467202758
41	23.512142298514046	19.130186295546153	32.851986283514144	24.505685122425653
42	22.516493873704054	14.798062847173481	39.258126616800695	23.427316662321775
43	25.530711693103658	19.39659494254718	35.79872460745583	19.273968756893336
44	19.29061303066156	22.228327350753002	35.59458158702148	22.886478031563957
45	22.50817173681994	19.652976918603486	34.85290873724106	22.985942607335513
46	27.728658230894176	15.968776946678165	34.38135440271121	21.92121041971645
47	19.881083682595705	15.336194276776224	39.37433573304992	25.408386307578155
48	20.478472737481702	13.927146208915717	34.068622535945615	31.525758517656964
49	22.989853009003948	13.584133795897085	39.73559668718792	23.690416507911046
50	21.080072994164478	13.370164637936913	40.1433813945094	25.406380973389215
51	18.084404516012594	12.764754246295146	38.60208153688812	30.54875970080414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	527.0
1	1740.0
2	2953.0
3	36839.5
4	70726.0
5	38815.0
6	6904.0
7	6371.0
8	5838.0
9	6066.5
10	6295.0
11	6452.0
12	6609.0
13	6404.5
14	6200.0
15	5792.5
16	5385.0
17	4813.5
18	4242.0
19	3866.0
20	3490.0
21	3282.0
22	3074.0
23	2877.0
24	2680.0
25	2575.5
26	2861.0
27	3251.0
28	3487.0
29	3723.0
30	4358.5
31	4994.0
32	5828.5
33	6663.0
34	7886.0
35	9109.0
36	11054.5
37	13000.0
38	14582.5
39	16165.0
40	19323.5
41	22482.0
42	28759.5
43	35037.0
44	41179.0
45	47321.0
46	56646.5
47	65972.0
48	90016.5
49	114061.0
50	144644.0
51	175227.0
52	169916.0
53	164605.0
54	127169.0
55	89733.0
56	67286.5
57	44840.0
58	33350.0
59	21860.0
60	16747.0
61	11634.0
62	9111.5
63	6589.0
64	5435.0
65	4281.0
66	3419.5
67	2558.0
68	2288.5
69	2019.0
70	1765.5
71	1512.0
72	1430.0
73	1348.0
74	1120.0
75	698.5
76	505.0
77	419.0
78	333.0
79	226.5
80	120.0
81	95.0
82	70.0
83	48.5
84	27.0
85	16.5
86	6.0
87	6.0
88	6.0
89	3.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.798189366538345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86854385687585	21.71350668711091
2	11.979198364282464	7.139168426364306
3	4.779533895751584	4.272643683281833
4	2.483777547272336	2.9604829479190893
5	1.443883209327779	2.1512552647357146
6	0.9271768361880378	1.6576914564599434
7	0.699778989207034	1.4596502784581193
8	0.4866690078122899	1.1601484202892756
9	0.3878377803709297	1.0401177260691787
>10	3.3226187498992923	21.2272567372094
>50	0.36484379411487083	7.344865252701517
>100	0.23643504821308078	13.26395914624884
>500	0.011549987987419885	2.228391295669884
>1k	0.006794110580835226	3.454138102214202
>5k	0.001019116587125284	2.345307437511381
>10k+	3.3970552904176134E-4	6.581417137756375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65017	6.51904064812401	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	8489	0.8511640964966812	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	8383	0.8405358252952855	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	6297	0.6313794693885736	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	3804	0.3814145627368801	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	3260	0.3268694727976417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	3152	0.31604066817735177	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	2015	0.20203741953596566	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1987	0.19922995167144605	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1872	0.18769928008502615	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1716	0.17205767341127398	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1666	0.16704433793891751	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1609	0.16132913550043115	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1407	0.141075260192111	No Hit
TGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	1332	0.13355525698357631	Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1308	0.13114885595684522	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC	1233	0.12362885274831051	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	1198	0.12011951791766097	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	1183	0.11861551727595403	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT	1162	0.11650991637756432	No Hit
GTAATGGGTTTACCTGGCTTCCTTCTTGGTACATAGGCTACTCTTCCACAC	1084	0.10868911304068823	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1071	0.10738564581787556	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG	1035	0.10377604427777888	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	1029	0.10317444402109613	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8714179718050014	0.0
2	0.0	0.0	0.0	2.9202679126476427	0.0
3	0.0	0.0	0.0	4.7823209737902825	0.0
4	0.0	0.0	0.0	5.938396133715684	0.0
5	0.0	0.0	0.0	11.811919706419074	0.0
6	0.0	0.0	0.0	13.358633966350492	0.0
7	0.0	0.0	0.0	18.47023081396515	0.0
8	0.0	0.0	0.0	24.78452684139812	0.0
9	0.0	0.0	0.0	29.569855816471815	0.0
10	0.0	0.0	0.0	32.44039144123368	0.0
11	0.0	0.0	0.0	34.93141757073816	0.0
12	0.0	0.0	0.0	36.66723484468687	0.0
13	0.0	0.0	0.0	37.376621814025306	0.0
14	0.0	0.0	0.0	37.88577616459783	0.0
15	0.0	0.0	0.0	39.468987506768	0.0
16	0.0	0.0	0.0	41.221549321194374	0.0
17	0.0	0.0	0.0	43.284236067940725	0.0
18	0.0	0.0	0.0	44.284095694547496	0.0
19	0.0	0.0	0.0	45.448292457938116	0.0
20	0.0	0.0	0.0	46.64898630356749	0.0
21	0.0	0.0	0.0	47.77748811839493	0.0
22	0.0	0.0	0.0	48.85996751358614	0.0
23	0.0	0.0	0.0	49.65307718531293	0.0
24	7.018669661299056E-4	0.0	0.0	50.352537750416104	0.0
25	7.018669661299056E-4	0.0	0.0	50.99243989010769	0.0
26	7.018669661299056E-4	0.0	0.0	51.653999639039846	0.0
27	7.018669661299056E-4	0.0	0.0	52.29380151202198	0.0
28	7.018669661299056E-4	0.0	0.0	52.91304870956745	0.0
29	7.018669661299056E-4	0.0	0.0	53.66174022900916	0.0
30	7.018669661299056E-4	0.0	0.0	54.30946317203762	0.0
31	7.018669661299056E-4	0.0	0.0	54.87015461126597	0.0
32	8.021336755770349E-4	0.0	0.0	55.44157458840516	0.0
33	8.021336755770349E-4	0.0	0.0	55.9850201536086	0.0
34	8.021336755770349E-4	0.0	0.0	56.618404957186115	0.0
35	8.021336755770349E-4	0.0	0.0	57.14741211622917	0.0
36	8.021336755770349E-4	0.0	0.0	57.625082720035294	0.0
37	8.021336755770349E-4	0.0	0.0	58.133134136803896	0.0
38	8.021336755770349E-4	0.0	0.0	58.61461487556901	0.0
39	8.021336755770349E-4	0.0	0.0	59.08586840997052	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	20	7.032836E-4	45.000004	44
CGGGTCC	40	6.8139343E-9	45.000004	5
AGTCCAC	20	7.032836E-4	45.000004	33
CAACCGG	35	1.2117198E-7	45.000004	1
ATTAACG	20	7.032836E-4	45.000004	9
TGCGGAT	20	7.032836E-4	45.000004	16
TGTGCGT	40	6.8139343E-9	45.000004	19
TAGCATA	20	7.032836E-4	45.000004	29
CACTCGC	35	1.2117198E-7	45.000004	21
TGTTCGG	20	7.032836E-4	45.000004	1
GGACGTA	20	7.032836E-4	45.000004	19
CATACCG	40	6.8139343E-9	45.000004	13
TCATCGG	45	3.8380676E-10	45.000004	1
CCGACAT	20	7.032836E-4	45.000004	9
GAGCATT	20	7.032836E-4	45.000004	8
GTCCACC	20	7.032836E-4	45.000004	34
CGATCTT	20	7.032836E-4	45.000004	9
TCGACCC	20	7.032836E-4	45.000004	23
TAGCTAA	20	7.032836E-4	45.000004	38
TAGTCGG	35	1.2117198E-7	45.000004	1
>>END_MODULE