##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043267_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2695336
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34094858674391	33.0	31.0	34.0	31.0	34.0
2	32.631470807350176	34.0	31.0	34.0	31.0	34.0
3	32.72703180605312	34.0	31.0	34.0	31.0	34.0
4	36.20507164969414	37.0	37.0	37.0	35.0	37.0
5	36.19183730711125	37.0	37.0	37.0	35.0	37.0
6	36.34724798689292	37.0	37.0	37.0	35.0	37.0
7	36.29912893976855	37.0	37.0	37.0	35.0	37.0
8	36.20158636993681	37.0	37.0	37.0	35.0	37.0
9	37.81348225230546	39.0	38.0	39.0	35.0	39.0
10	37.596136066152795	39.0	37.0	39.0	35.0	39.0
11	37.25416274631437	39.0	37.0	39.0	35.0	39.0
12	37.261278742242155	39.0	37.0	39.0	35.0	39.0
13	37.26725758866427	39.0	37.0	39.0	35.0	39.0
14	38.55586131005559	40.0	37.0	41.0	35.0	41.0
15	38.605750080880455	40.0	37.0	41.0	35.0	41.0
16	38.488786555739246	40.0	37.0	41.0	34.0	41.0
17	38.42726138781955	40.0	37.0	41.0	34.0	41.0
18	38.28421206113078	40.0	37.0	41.0	34.0	41.0
19	38.09885075552732	40.0	37.0	41.0	34.0	41.0
20	37.843869929389136	40.0	35.0	41.0	34.0	41.0
21	37.785550298738265	40.0	35.0	41.0	34.0	41.0
22	37.75248577542837	40.0	35.0	41.0	34.0	41.0
23	37.741785068726124	39.0	35.0	41.0	34.0	41.0
24	37.66045754592378	39.0	35.0	41.0	34.0	41.0
25	37.62685728235738	39.0	35.0	41.0	34.0	41.0
26	37.59872869282346	39.0	35.0	41.0	34.0	41.0
27	37.58014622295699	39.0	35.0	41.0	34.0	41.0
28	37.49522508510998	39.0	35.0	41.0	34.0	41.0
29	37.413405601379566	39.0	35.0	41.0	34.0	41.0
30	37.30766108566798	39.0	35.0	41.0	33.0	41.0
31	37.19665043616084	39.0	35.0	41.0	33.0	41.0
32	36.95769321524293	39.0	35.0	41.0	33.0	41.0
33	36.53856847532182	39.0	35.0	41.0	31.0	41.0
34	36.25508656434671	39.0	35.0	41.0	31.0	41.0
35	36.10778544864166	39.0	35.0	41.0	30.0	41.0
36	36.06021030402147	39.0	35.0	41.0	31.0	41.0
37	35.93786859968479	38.0	35.0	41.0	30.0	41.0
38	35.837920392856404	38.0	35.0	41.0	30.0	41.0
39	35.74522879522256	38.0	35.0	41.0	30.0	41.0
40	35.63599862874239	38.0	35.0	40.0	30.0	41.0
41	35.527704152654806	38.0	35.0	40.0	29.0	41.0
42	35.43409578620253	37.0	35.0	40.0	29.0	41.0
43	35.32470942398276	37.0	35.0	40.0	29.0	41.0
44	35.213975919885314	37.0	35.0	40.0	28.0	41.0
45	35.171358227694064	37.0	35.0	40.0	28.0	41.0
46	35.10385458436351	37.0	35.0	40.0	27.0	41.0
47	34.97187178147734	36.0	35.0	40.0	27.0	41.0
48	34.89974941899637	36.0	35.0	40.0	27.0	41.0
49	34.82835794869359	36.0	35.0	40.0	27.0	41.0
50	34.76278690300578	36.0	35.0	40.0	27.0	41.0
51	34.333015253014835	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	2.0
13	2.0
14	28.0
15	39.0
16	86.0
17	210.0
18	448.0
19	932.0
20	1694.0
21	2854.0
22	4348.0
23	6863.0
24	11205.0
25	19983.0
26	32183.0
27	42631.0
28	46319.0
29	46917.0
30	49825.0
31	55511.0
32	64051.0
33	83553.0
34	203609.0
35	274924.0
36	240767.0
37	250611.0
38	396454.0
39	859044.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.545741235972063	17.290571565103573	23.73373857656337	29.429948622360996
2	35.36802090722641	19.747371014226054	23.923325329383797	20.96128274916374
3	26.37589525016547	20.747209253317582	33.36270505792228	19.51419043859467
4	23.500075686296626	23.354676374299903	31.3578715232535	21.78737641614997
5	22.104442637207384	30.944973094263574	28.477562723163274	18.47302154536577
6	78.48502004944838	2.106787428357726	16.541425633019408	2.8667668891744853
7	78.0672613729791	1.9748929261509511	15.291971019568617	4.66587468130133
8	68.53913575153524	3.4646515313860684	20.75522309648964	7.240989620589048
9	42.73096192830875	14.48795252243134	25.147959289676685	17.63312625958322
10	30.500872618478738	15.141785662344137	34.409216513265875	19.94812520591125
11	26.909149731239445	12.640835873523745	38.756800636358506	21.6932137588783
12	21.71714398501708	13.552262129842068	41.23100051347958	23.49959337166127
13	17.290274756097197	16.314440945396047	43.73172769554519	22.66355660296156
14	18.4198556320993	16.505511743248338	43.36643001095225	21.70820261370011
15	22.63239165729245	13.980483323785977	41.832224257012854	21.55490076190872
16	21.25538337335308	17.730182804667024	39.0989101173286	21.915523704651292
17	21.620458451191244	19.478165245446206	38.536865162636495	20.364511140726055
18	21.937747279003432	25.61647230623566	34.176777960150424	18.269002454610483
19	22.92734560737511	21.759624774054142	32.84759302736282	22.465436591207926
20	22.241865207157847	25.914765357640015	33.30367716677995	18.539692268422193
21	19.27930321117664	20.400239524868145	35.46248037350445	24.857976890450765
22	21.786671494759837	21.018566887393632	33.360998406135636	23.833763211710895
23	23.029262399938265	19.074245288899046	35.27044494638145	22.626047364781236
24	24.063975697278558	17.939358951908034	33.111641739656946	24.885023611156456
25	18.296086276441972	18.467159567489915	37.16538494644082	26.0713692096273
26	18.041646755729158	17.964365110694917	36.92879106723615	27.06519706633978
27	22.181909787870605	20.773254243626766	34.83354208900115	22.21129387950148
28	24.81779637121309	21.54518026695002	31.077312809979908	22.559710551856984
29	23.5704565219327	18.665613489375723	35.74782513200581	22.01610485668577
30	28.013947055209442	18.093959343102306	31.244156572687043	22.64793702900121
31	29.73254540435775	16.942377499502843	31.16227438805403	22.162802708085376
32	28.62548491171416	18.59604887850717	30.562905700810582	22.215560508968085
33	25.976983945600846	18.45361765657417	34.140715665876165	21.42868273194882
34	25.498824636334767	21.989280742734856	32.52588916558084	19.986005455349538
35	27.148229385872487	24.71936708447481	28.280110531673973	19.85229299797873
36	27.1685979039348	22.942037653190546	28.141834635830186	21.747529807044465
37	25.95709774217389	23.641727784587896	29.784857991730902	20.616316481507315
38	26.63964715345322	20.125876699602575	29.004547113977626	24.229929032966577
39	27.913625611055544	17.499302498835025	32.549077369203694	22.037994520905745
40	23.329410507632446	18.16211411119059	34.29342389965481	24.215051481522153
41	23.629410210823437	21.210676516768228	30.389124027579495	24.770789244828844
42	22.690046806780305	17.002592626670666	32.07366354324655	28.233697023302472
43	24.37725760350472	18.276422679769794	33.06329897274403	24.283020743981456
44	23.58355321933889	19.64623334530463	33.34556433780427	23.424649097552216
45	24.13710201622358	20.531503307936376	32.72686596402081	22.60452871181923
46	24.44467034907707	18.9668746308438	32.283099398368144	24.305355621710987
47	22.57996776654191	19.338813416954324	34.13674584541593	23.944472971087833
48	24.00676576130026	17.935611738202585	31.04852233636177	27.00910016413538
49	22.725849393173984	16.46662976341354	34.799038042010345	26.008482801402128
50	21.97039626970441	16.46451499924314	36.81856362249456	24.746525108557893
51	20.55109270235696	15.467236737831572	35.800694236265905	28.180976323545558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	669.0
1	4326.5
2	7984.0
3	84223.0
4	160462.0
5	91985.0
6	23508.0
7	20567.5
8	17627.0
9	16938.5
10	16250.0
11	15720.5
12	15191.0
13	14411.0
14	13631.0
15	12658.0
16	11685.0
17	10663.5
18	9642.0
19	9121.5
20	8601.0
21	8311.5
22	8022.0
23	8101.5
24	8181.0
25	8743.5
26	9965.0
27	10624.0
28	11014.5
29	11405.0
30	13731.0
31	16057.0
32	16999.5
33	17942.0
34	21827.0
35	25712.0
36	27787.5
37	29863.0
38	33260.0
39	36657.0
40	40278.0
41	43899.0
42	51838.0
43	59777.0
44	70306.0
45	80835.0
46	93782.5
47	106730.0
48	141515.5
49	176301.0
50	219812.5
51	263324.0
52	279155.5
53	294987.0
54	267570.0
55	240153.0
56	214441.0
57	188729.0
58	169421.5
59	150114.0
60	144707.0
61	139300.0
62	137767.0
63	136234.0
64	130144.0
65	124054.0
66	101449.5
67	78845.0
68	64134.5
69	49424.0
70	41664.0
71	33904.0
72	31316.5
73	28729.0
74	24340.5
75	15298.0
76	10644.0
77	8054.5
78	5465.0
79	4103.5
80	2742.0
81	2036.0
82	1330.0
83	905.0
84	480.0
85	305.5
86	131.0
87	135.0
88	139.0
89	90.0
90	41.0
91	38.5
92	36.0
93	25.5
94	15.0
95	9.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2695336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.563268088114228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8343973325897	20.075540197144015
2	10.551761508216597	5.816820625056371
3	3.9572192313858308	3.2722168367438695
4	1.9649521896087276	2.166420159300497
5	1.2184177691506006	1.6791787807210037
6	0.9118636830110655	1.5080365892789527
7	0.7140450500718418	1.3776990599484789
8	0.5695862116092952	1.2559725959904293
9	0.523953785898128	1.2997690799843258
>10	6.201164631084601	36.20921909788407
>50	0.3685444732336169	6.766971171763098
>100	0.17121821955615868	8.415636914372827
>500	0.006912333092443383	1.3105233870899864
>1k	0.0051503658335852655	2.725462762404503
>5k	4.066078289672578E-4	0.7974093984445769
>10k+	4.066078289672578E-4	5.323123343873007
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	115068	4.2691523431587015	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16266	0.603486912206864	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	11155	0.4138630582606399	No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	8904	0.3303484240925807	No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT	7233	0.26835244288652693	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG	5208	0.19322266314849057	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	4636	0.1720008191928576	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4058	0.15055636848244522	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3703	0.137385468824666	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	3550	0.13170899657779217	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	3512	0.13029915379752283	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT	3007	0.11156308527025943	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	2829	0.10495908487847154	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2757	0.10228780382111914	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2754758590394667	0.0
2	0.0	0.0	0.0	0.9070112223485309	0.0
3	0.0	0.0	0.0	1.5143195505124407	0.0
4	0.0	0.0	0.0	1.9285165189052496	0.0
5	0.0	0.0	0.0	4.1119548731586715	0.0
6	0.0	0.0	0.0	5.003865937308002	0.0
7	0.0	0.0	0.0	7.549485481587453	0.0
8	0.0	0.0	0.0	10.607768382123787	0.0
9	0.0	0.0	0.0	13.7040428354758	0.0
10	0.0	0.0	0.0	15.341204213500655	0.0
11	0.0	0.0	0.0	16.539607677855376	0.0
12	0.0	0.0	0.0	17.362473546897306	0.0
13	0.0	0.0	0.0	17.80746444970126	0.0
14	0.0	0.0	0.0	18.089210399000347	0.0
15	0.0	0.0	0.0	18.77513601272717	0.0
16	0.0	0.0	0.0	19.512780595814398	0.0
17	0.0	0.0	0.0	20.441570179005513	0.0
18	0.0	0.0	0.0	21.001945583036772	0.0
19	0.0	0.0	0.0	21.637562070183456	0.0
20	0.0	0.0	0.0	22.339663774757582	0.0
21	0.0	0.0	0.0	23.034679164304563	0.0
22	3.710112579656117E-5	0.0	0.0	23.68009034866154	0.0
23	3.710112579656117E-5	0.0	0.0	24.27508110306099	0.0
24	1.1130337738968351E-4	0.0	0.0	24.75338881683026	0.0
25	1.1130337738968351E-4	0.0	0.0	25.226057159478447	0.0
26	1.484045031862447E-4	0.0	0.0	25.68551750134306	0.0
27	1.484045031862447E-4	0.0	0.0	26.18482445231318	0.0
28	1.484045031862447E-4	0.0	0.0	26.66602605389458	0.0
29	1.484045031862447E-4	0.0	0.0	27.163737656455446	0.0
30	1.484045031862447E-4	0.0	0.0	27.63217647076283	0.0
31	1.484045031862447E-4	0.0	0.0	28.133264275771182	0.0
32	1.484045031862447E-4	0.0	0.0	28.67891053286121	0.0
33	1.484045031862447E-4	0.0	0.0	29.172318404829674	0.0
34	1.484045031862447E-4	0.0	0.0	29.70279030146891	0.0
35	1.484045031862447E-4	0.0	0.0	30.18440001543407	0.0
36	1.484045031862447E-4	0.0	0.0	30.713647574922014	0.0
37	1.484045031862447E-4	0.0	0.0	31.40187345844822	0.0
38	1.484045031862447E-4	0.0	0.0	32.097111454749985	0.0
39	1.484045031862447E-4	0.0	0.0	32.91411534591606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28195	0.0	44.24189	1
AATACGG	165	0.0	43.636364	1
ACTACGG	150	0.0	42.0	1
TAGTAGG	630	0.0	41.785713	1
AGACACG	445	0.0	40.95506	23
TGCAACG	105	0.0	40.714283	11
CACTTAC	4590	0.0	40.539215	36
ACCCGCA	4675	0.0	40.331554	31
CGCACTT	4630	0.0	40.23758	34
ACTTACT	4600	0.0	40.157608	37
GCACTTA	4635	0.0	40.14563	35
CCGCACT	4645	0.0	40.10764	33
CATGCGG	365	0.0	40.068493	1
CTTACTG	4635	0.0	39.757282	38
GCGATAT	85	0.0	39.705883	8
AGGGCGA	1095	0.0	39.65753	5
CCCGCAC	4765	0.0	39.616997	32
CGGGTAC	415	0.0	39.578312	5
TACGGGA	490	0.0	39.489796	3
GTTTTTT	32445	0.0	39.39667	2
>>END_MODULE