FastQCFastQC Report
Thu 26 May 2016
SRR1043266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043266_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences925491
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT678737.333728799091508No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT77060.8326391072414535RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT74290.8027090484942587TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT54270.5863914397870967Illumina PCR Primer Index 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC34850.3765568762959337TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG26400.2852539895039498No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT26350.2847137357359499Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC23170.2503535960911559TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC19260.20810575143356339Illumina PCR Primer Index 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT17490.18898076804636674TruSeq Adapter, Index 10 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC15930.17212485048476972TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC14860.16056341984957173TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT14800.15991511532797187TruSeq Adapter, Index 10 (95% over 24bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTTG12030.12998505658077714Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT12020.12987700582717715No Hit
CGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG11850.12804014301597746TruSeq Adapter, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC11660.12598717869757783TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT11270.12177319930717857No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC10780.1164787123807795No Hit
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC10420.11258888525118019RNA PCR Primer, Index 10 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC10090.1090232103823808TruSeq Adapter, Index 10 (95% over 23bp)
CGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG10040.1084829566143809TruSeq Adapter, Index 10 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC10010.10815880435358095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTG206.310815E-446.018
CATATGT206.310815E-446.035
ACGCATA206.310815E-446.026
GGCACTA206.310815E-446.07
TGGACGA206.310815E-446.09
ATCAGTC206.310815E-446.017
GACAAGT301.8610081E-646.029
ATTACGG453.092282E-1046.01
TAAACGG405.6097633E-946.01
TGAACGG600.046.01
TGTATGC253.416636E-546.01
AGGGTGC453.092282E-1046.05
GGTCACA206.310815E-446.07
GCGTTCC301.8610081E-646.039
TATGCAA206.310815E-446.09
TAGTTTC301.8610081E-646.018
AACGGCC1000.046.05
ACGATGG253.416636E-546.01
GCCGATG301.8610081E-646.08
CTTGCGG1850.046.01
Produced by FastQC (version 0.11.5)