##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043266_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	925491
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25272963216282	33.0	31.0	34.0	31.0	34.0
2	32.573923463329194	34.0	31.0	34.0	31.0	34.0
3	32.637897073013136	34.0	31.0	34.0	31.0	34.0
4	36.15931219212288	37.0	35.0	37.0	35.0	37.0
5	36.05758024659343	37.0	35.0	37.0	35.0	37.0
6	36.30135355179035	37.0	37.0	37.0	35.0	37.0
7	36.22660944298756	37.0	37.0	37.0	35.0	37.0
8	36.20724134540476	37.0	37.0	37.0	35.0	37.0
9	37.767621727277735	39.0	38.0	39.0	35.0	39.0
10	37.62742371346669	39.0	37.0	39.0	35.0	39.0
11	37.354488590380676	39.0	37.0	39.0	35.0	39.0
12	37.347381011808864	39.0	37.0	39.0	34.0	39.0
13	37.34972571316199	39.0	37.0	39.0	35.0	39.0
14	38.769218717415946	40.0	38.0	41.0	35.0	41.0
15	38.86290952586249	41.0	38.0	41.0	35.0	41.0
16	38.87723489477477	41.0	38.0	41.0	35.0	41.0
17	38.863775012398825	41.0	38.0	41.0	35.0	41.0
18	38.5782368494129	40.0	38.0	41.0	35.0	41.0
19	38.31301114759625	40.0	37.0	41.0	35.0	41.0
20	38.01864847956382	40.0	35.0	41.0	34.0	41.0
21	37.95310921446022	40.0	35.0	41.0	34.0	41.0
22	37.90644749651806	40.0	35.0	41.0	34.0	41.0
23	37.91127628469645	40.0	35.0	41.0	34.0	41.0
24	37.83065313439029	40.0	35.0	41.0	34.0	41.0
25	37.74522928910168	40.0	35.0	41.0	34.0	41.0
26	37.71009442555357	40.0	35.0	41.0	34.0	41.0
27	37.75167667756899	40.0	35.0	41.0	34.0	41.0
28	37.78427991196025	40.0	35.0	41.0	34.0	41.0
29	37.717351114165346	40.0	35.0	41.0	34.0	41.0
30	37.684644151050634	40.0	35.0	41.0	34.0	41.0
31	37.5442516458831	40.0	35.0	41.0	34.0	41.0
32	37.39149921501127	40.0	35.0	41.0	33.0	41.0
33	37.227147535740485	40.0	35.0	41.0	33.0	41.0
34	37.00857922983584	40.0	35.0	41.0	33.0	41.0
35	36.90584025128283	40.0	35.0	41.0	33.0	41.0
36	36.8232700263968	40.0	35.0	41.0	32.0	41.0
37	36.77759048980487	40.0	35.0	41.0	32.0	41.0
38	36.72250405460453	40.0	35.0	41.0	32.0	41.0
39	36.69320393175082	40.0	35.0	41.0	32.0	41.0
40	36.66844302105585	39.0	35.0	41.0	32.0	41.0
41	36.65528135875984	40.0	35.0	41.0	32.0	41.0
42	36.56881374319145	39.0	35.0	41.0	32.0	41.0
43	36.453377720582914	39.0	35.0	41.0	31.0	41.0
44	36.30932121436081	39.0	35.0	41.0	31.0	41.0
45	36.2480769667128	39.0	35.0	41.0	31.0	41.0
46	36.232365306631834	39.0	35.0	41.0	31.0	41.0
47	36.15280213421848	39.0	35.0	41.0	31.0	41.0
48	36.13160689839231	39.0	35.0	41.0	31.0	41.0
49	36.09764654653584	39.0	35.0	41.0	31.0	41.0
50	35.973440044257586	39.0	35.0	41.0	30.0	41.0
51	35.8871734030909	39.0	35.0	41.0	30.0	41.0
52	35.574980199699404	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	2.0
15	11.0
16	24.0
17	44.0
18	131.0
19	258.0
20	455.0
21	791.0
22	1310.0
23	2283.0
24	4227.0
25	7693.0
26	10938.0
27	11762.0
28	11015.0
29	10993.0
30	12200.0
31	15171.0
32	19158.0
33	25561.0
34	46096.0
35	90838.0
36	99692.0
37	69802.0
38	111536.0
39	371350.0
40	2145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.200470885184192	19.531470322239763	25.11142733964998	29.156631452926067
2	33.91086461132523	19.725853627966128	26.557686676585725	19.805595084122913
3	21.037265624409095	21.944135599373737	38.23570407491807	18.782894701299092
4	22.1471629653881	23.637615060546242	36.107320330505644	18.107901643560012
5	19.576095283476555	29.079591265609285	31.484368837730457	19.859944613183707
6	70.81505924963074	1.5771087995453223	23.07110495942154	4.536726991402402
7	71.40458416127224	1.3536598411005618	18.856369213747083	8.385386783880124
8	58.26064218884894	3.175179445289041	28.276774166361424	10.287404199500589
9	31.113106448360924	19.457347505270175	30.56312811253702	18.86641793383188
10	23.97462536102458	18.325515861310375	45.087202360692864	12.612656416972179
11	21.770281936831367	15.573679268626059	41.00482878817838	21.651210006364188
12	16.008583551865982	20.895827187946722	44.65219002669934	18.443399233487952
13	11.602165769305158	24.148911226581347	43.33872506593797	20.91019793817552
14	9.616733171905507	22.976992753035955	46.6128790015246	20.79339507353394
15	10.199342835316605	18.41541408830556	49.10463743029376	22.28060564608408
16	10.013927742139037	25.813000882774656	40.56214485067926	23.610926524407045
17	12.591154317005785	22.83404160602318	42.0203978212646	22.55440625570643
18	14.03568484188393	29.671277192322776	39.74474089969541	16.548297066097888
19	13.999163687167135	23.80412127184381	37.20414353029905	24.992571510690002
20	11.388333327930795	31.607438646080837	35.2034757766418	21.800752249346562
21	12.930973937077725	24.620228613784466	36.557027566988765	25.891769882149042
22	10.467740907258957	27.62868574626874	32.89983370989021	29.003739636582093
23	10.325221963260582	23.52059609439746	44.01771600156025	22.136465940781704
24	16.510479302337895	21.890218273327346	38.91620772109075	22.68309470324401
25	11.490009087068378	26.134451874734598	38.81518026647477	23.560358771722253
26	14.395926054386265	22.05434736804572	38.311879856206055	25.237846721361954
27	20.893882274381923	23.07164521318954	38.821015007169166	17.21345750525937
28	15.22219016716532	23.05554565090314	34.87392097816186	26.848343203769677
29	16.02338650510918	23.444636414616674	38.823716276009165	21.708260804264977
30	23.767816218634216	21.87487506631615	32.90696505962781	21.450343655421825
31	17.46154203552493	25.62596502829309	35.25296302179059	21.65952991439139
32	17.262836699654564	21.631976972223395	39.16267149005231	21.942514838069737
33	15.975195869003588	21.551155008530607	40.24512393961692	22.228525182848887
34	15.45590394720208	26.609875190574517	37.510467416755	20.423753445468407
35	15.430079817091686	21.98314192142333	40.46241400510648	22.124364256378506
36	14.240008816941494	24.59905066607887	38.650402867234796	22.510537649744837
37	13.597647086789607	25.587715061518697	39.936855139596176	20.877782712095524
38	12.26808256374184	24.20163999433814	32.985409906741396	30.54486753517862
39	18.1966113122656	19.3178539823726	36.58436440764956	25.90117029771224
40	13.251236370748067	17.034741558805003	41.685008282090266	28.029013788356664
41	17.978889043761637	22.029711796224923	32.46979171056228	27.521607449451157
42	19.064150812919845	17.350249759316945	38.08821479625409	25.497384631509114
43	21.457150852898625	21.219547245732265	36.84422647005752	20.479075431311596
44	14.243682542563892	24.36717374885331	38.558559726674815	22.83058398190798
45	17.754791780795276	21.536892309055407	36.897279390075106	23.811036520074207
46	21.717337067567378	18.86263615745588	35.32762609252818	24.09240068244856
47	15.268651991213314	16.87461034196983	40.040259710791354	27.816477956025505
48	16.963860264443415	15.455255642680479	34.27359099116037	33.30729310171574
49	18.900453921215874	15.438507775872482	40.843617063807216	24.81742123910443
50	16.880337031910628	14.732504151850206	41.114932506096764	27.2722263101424
51	14.65557201528702	14.402192998095064	39.61702490894023	31.325210077677685
52	13.490028536204028	14.26907446965989	47.970752822015555	24.270144172120528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	1565.0
2	2860.0
3	37785.5
4	72711.0
5	39076.0
6	5441.0
7	5167.5
8	4894.0
9	5243.0
10	5592.0
11	5857.0
12	6122.0
13	6108.5
14	5783.0
15	5471.0
16	4975.0
17	4479.0
18	4068.5
19	3658.0
20	3490.0
21	3322.0
22	3036.5
23	2751.0
24	2893.0
25	3035.0
26	3477.5
27	3920.0
28	4457.0
29	4994.0
30	5182.0
31	5370.0
32	6186.0
33	7002.0
34	8744.5
35	10487.0
36	12374.0
37	14261.0
38	16403.5
39	22422.0
40	26298.0
41	38240.0
42	50182.0
43	72121.0
44	94060.0
45	121408.5
46	148757.0
47	151269.5
48	153782.0
49	129699.5
50	105617.0
51	81213.5
52	56810.0
53	43811.0
54	30812.0
55	24714.5
56	18617.0
57	16401.5
58	14186.0
59	12290.5
60	10395.0
61	8957.5
62	7520.0
63	6430.5
64	4438.5
65	3536.0
66	2879.5
67	2223.0
68	1915.0
69	1607.0
70	1401.0
71	1195.0
72	1333.0
73	1471.0
74	1177.0
75	883.0
76	659.0
77	435.0
78	367.0
79	299.0
80	198.0
81	97.0
82	73.5
83	50.0
84	40.5
85	31.0
86	17.5
87	4.0
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	925491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.226016206447873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58666848780783	20.488324811089228
2	11.874085593290788	6.703162647859501
3	4.853249239349521	4.109636750514311
4	2.4640429943924196	2.782004699724191
5	1.4442143857159178	2.038220932840133
6	0.9526359640251417	1.6133470895651247
7	0.6638748944751585	1.3116980471356783
8	0.5084519988371777	1.1481259487503197
9	0.39160938193119177	0.9948215484888168
>10	3.503366876901479	21.80900684045744
>50	0.49289692442080635	9.373802200080052
>100	0.2445260850947314	12.583556416002645
>500	0.01153424929692129	2.155897440021979
>1k	0.007305024554716817	3.291254281736966
>5k	0.001153424929692129	2.231428730581492
>10k+	3.8447497656404295E-4	7.365711615152107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67873	7.333728799091508	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	7706	0.8326391072414535	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	7429	0.8027090484942587	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	5427	0.5863914397870967	Illumina PCR Primer Index 2 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3485	0.3765568762959337	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	2640	0.2852539895039498	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	2635	0.2847137357359499	Illumina PCR Primer Index 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2317	0.2503535960911559	TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1926	0.20810575143356339	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1749	0.18898076804636674	TruSeq Adapter, Index 10 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1593	0.17212485048476972	TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1486	0.16056341984957173	TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1480	0.15991511532797187	TruSeq Adapter, Index 10 (95% over 24bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTTG	1203	0.12998505658077714	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1202	0.12987700582717715	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1185	0.12804014301597746	TruSeq Adapter, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1166	0.12598717869757783	TruSeq Adapter, Index 10 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	1127	0.12177319930717857	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1078	0.1164787123807795	No Hit
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1042	0.11258888525118019	RNA PCR Primer, Index 10 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1009	0.1090232103823808	TruSeq Adapter, Index 10 (95% over 23bp)
CGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1004	0.1084829566143809	TruSeq Adapter, Index 10 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	1001	0.10815880435358095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8212937781134555	0.0
2	0.0	0.0	0.0	2.7304425434715194	0.0
3	0.0	0.0	0.0	4.586322287304792	0.0
4	0.0	0.0	0.0	5.753594578445387	0.0
5	0.0	0.0	0.0	11.609945423564357	0.0
6	0.0	0.0	0.0	13.18240804070488	0.0
7	0.0	0.0	0.0	18.23983161370559	0.0
8	0.0	0.0	0.0	24.171601884837347	0.0
9	0.0	0.0	0.0	28.769053399762935	0.0
10	0.0	0.0	0.0	31.332989731936884	0.0
11	0.0	0.0	0.0	33.723072401568466	0.0
12	0.0	0.0	0.0	35.42281880644977	0.0
13	0.0	0.0	0.0	36.101161437550445	0.0
14	0.0	0.0	0.0	36.556271211713565	0.0
15	0.0	0.0	0.0	38.0397000078877	0.0
16	0.0	0.0	0.0	39.82037642721539	0.0
17	0.0	0.0	0.0	41.86891066471743	0.0
18	0.0	0.0	0.0	42.817380179818066	0.0
19	0.0	0.0	0.0	43.91766100372667	0.0
20	0.0	0.0	0.0	44.97796304880328	0.0
21	0.0	0.0	0.0	46.0043371572495	0.0
22	2.1610150719996197E-4	0.0	0.0	47.04648667572132	0.0
23	2.1610150719996197E-4	0.0	0.0	47.82855803027798	0.0
24	0.0015127105503997338	0.0	0.0	48.47275662324107	0.0
25	0.0015127105503997338	0.0	0.0	49.05493408363777	0.0
26	0.0015127105503997338	0.0	0.0	49.62911578826807	0.0
27	0.0015127105503997338	0.0	0.0	50.18255174820717	0.0
28	0.0015127105503997338	0.0	0.0	50.74560422521667	0.0
29	0.0015127105503997338	0.0	0.0	51.43259091660535	0.0
30	0.0015127105503997338	0.0	0.0	52.014660326248446	0.0
31	0.0015127105503997338	0.0	0.0	52.492028555653164	0.0
32	0.0015127105503997338	0.0	0.0	52.98225482473627	0.0
33	0.0015127105503997338	0.0	0.0	53.47302134758739	0.0
34	0.0015127105503997338	0.0	0.0	54.05617126476648	0.0
35	0.0015127105503997338	0.0	0.0	54.49388486760001	0.0
36	0.0015127105503997338	0.0	0.0	54.91236543629274	0.0
37	0.0017288120575996958	0.0	0.0	55.36671885518066	0.0
38	0.0019449135647996577	0.0	0.0	55.80475661027498	0.0
39	0.0019449135647996577	0.0	0.0	56.2425782638621	0.0
40	0.0019449135647996577	0.0	0.0	56.69617532747482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTG	20	6.310815E-4	46.0	18
CATATGT	20	6.310815E-4	46.0	35
ACGCATA	20	6.310815E-4	46.0	26
GGCACTA	20	6.310815E-4	46.0	7
TGGACGA	20	6.310815E-4	46.0	9
ATCAGTC	20	6.310815E-4	46.0	17
GACAAGT	30	1.8610081E-6	46.0	29
ATTACGG	45	3.092282E-10	46.0	1
TAAACGG	40	5.6097633E-9	46.0	1
TGAACGG	60	0.0	46.0	1
TGTATGC	25	3.416636E-5	46.0	1
AGGGTGC	45	3.092282E-10	46.0	5
GGTCACA	20	6.310815E-4	46.0	7
GCGTTCC	30	1.8610081E-6	46.0	39
TATGCAA	20	6.310815E-4	46.0	9
TAGTTTC	30	1.8610081E-6	46.0	18
AACGGCC	100	0.0	46.0	5
ACGATGG	25	3.416636E-5	46.0	1
GCCGATG	30	1.8610081E-6	46.0	8
CTTGCGG	185	0.0	46.0	1
>>END_MODULE