SRR1043265_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043265_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	906989
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62222	6.860281657219658	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	7540	0.83132209982701	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	7266	0.8011122516370098	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5482	0.6044174736408049	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	3487	0.3844589074398918	TruSeq Adapter, Index 10 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2681	0.29559344159631484	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	2505	0.27618857560565785	No Hit
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	2274	0.2507196889929205	TruSeq Adapter, Index 10 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1969	0.21709193827047515	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1705	0.18798463928448966	No Hit
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1658	0.18280265802562104	TruSeq Adapter, Index 10 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	1416	0.15612096728846767	Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1416	0.15612096728846767	TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1394	0.15369535903963555	TruSeq Adapter, Index 10 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1141	0.1258008641780661	RNA PCR Primer, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1140	0.12569060925766465	TruSeq Adapter, Index 10 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1117	0.12315474608843106	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	1115	0.12293423624762814	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1083	0.11940607879478142	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	1067	0.11764200006835804	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	943	0.10397038993857698	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	937	0.10330886041616821	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	929	0.10242682105295654	No Hit
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	907	0.10000121280412443	TruSeq Adapter, Index 10 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.032498E-4	45.000004	1
CCTTCGC	40	6.8121153E-9	45.000004	42
CTTCGCG	40	6.8121153E-9	45.000004	43
AACAATG	20	7.032498E-4	45.000004	20
GGTACTA	70	0.0	45.000004	7
GATCGGA	20	7.032498E-4	45.000004	8
CGGCCTA	20	7.032498E-4	45.000004	5
GGCACGG	75	0.0	45.000004	1
AGCTTAC	20	7.032498E-4	45.000004	10
CGTTGAA	20	7.032498E-4	45.000004	11
ATTACGG	75	0.0	45.000004	1
CCGGAGG	20	7.032498E-4	45.000004	1
CGCGCAG	30	2.164994E-6	45.000004	34
TGAACCG	20	7.032498E-4	45.000004	12
CTAAGTG	20	7.032498E-4	45.000004	23
CACTAGG	35	1.2115561E-7	45.000004	1
GAGCGAT	20	7.032498E-4	45.000004	18
CGAGGGT	40	6.8121153E-9	45.000004	3
TCTCGCG	30	2.164994E-6	45.000004	31
GAACGTA	20	7.032498E-4	45.000004	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content