##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043265_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	906989
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19159659047684	33.0	31.0	34.0	30.0	34.0
2	32.4812969065777	34.0	31.0	34.0	31.0	34.0
3	32.5379006801626	34.0	31.0	34.0	31.0	34.0
4	36.112735656110495	37.0	35.0	37.0	35.0	37.0
5	36.0655542680231	37.0	35.0	37.0	35.0	37.0
6	36.304066532229164	37.0	37.0	37.0	35.0	37.0
7	36.23875151738334	37.0	37.0	37.0	35.0	37.0
8	36.144912452080455	37.0	37.0	37.0	35.0	37.0
9	37.70748377323209	39.0	38.0	39.0	35.0	39.0
10	37.567473254912684	39.0	37.0	39.0	35.0	39.0
11	37.343270976825515	39.0	37.0	39.0	35.0	39.0
12	37.345516869554096	39.0	37.0	39.0	34.0	39.0
13	37.358405669749025	39.0	37.0	39.0	35.0	39.0
14	38.77059589476829	40.0	38.0	41.0	35.0	41.0
15	38.87943734709021	40.0	38.0	41.0	35.0	41.0
16	38.92174216004825	41.0	38.0	41.0	35.0	41.0
17	38.88873073433085	41.0	38.0	41.0	35.0	41.0
18	38.64103092760772	40.0	38.0	41.0	35.0	41.0
19	38.405885848670714	40.0	37.0	41.0	35.0	41.0
20	38.130534107910904	40.0	35.0	41.0	35.0	41.0
21	38.06979687736015	40.0	35.0	41.0	35.0	41.0
22	38.0366906324112	40.0	35.0	41.0	34.0	41.0
23	38.00966384377319	40.0	35.0	41.0	34.0	41.0
24	37.93561002393634	40.0	35.0	41.0	34.0	41.0
25	37.909310917772984	40.0	35.0	41.0	34.0	41.0
26	37.913335222367635	40.0	35.0	41.0	34.0	41.0
27	37.91540801487118	40.0	35.0	41.0	34.0	41.0
28	37.8683115230725	40.0	35.0	41.0	34.0	41.0
29	37.870982999793824	40.0	35.0	41.0	34.0	41.0
30	37.79850913296633	40.0	35.0	41.0	34.0	41.0
31	37.652766461335254	40.0	35.0	41.0	34.0	41.0
32	37.52325882673329	40.0	35.0	41.0	34.0	41.0
33	37.320975226821936	40.0	35.0	41.0	33.0	41.0
34	37.05205245047073	40.0	35.0	41.0	33.0	41.0
35	36.949241942294776	40.0	35.0	41.0	33.0	41.0
36	36.84479194345246	40.0	35.0	41.0	33.0	41.0
37	36.754049938863645	40.0	35.0	41.0	32.0	41.0
38	36.68501051280666	40.0	35.0	41.0	32.0	41.0
39	36.60402276102577	39.0	35.0	41.0	31.0	41.0
40	36.55908285547013	39.0	35.0	41.0	32.0	41.0
41	36.51887950129495	39.0	35.0	41.0	31.0	41.0
42	36.42133366556816	39.0	35.0	41.0	31.0	41.0
43	36.2950939868069	39.0	35.0	41.0	31.0	41.0
44	36.2106717942555	39.0	35.0	41.0	31.0	41.0
45	36.175773906850026	39.0	35.0	41.0	31.0	41.0
46	36.09174422181526	39.0	35.0	41.0	30.0	41.0
47	35.95766211056584	38.0	35.0	40.0	30.0	41.0
48	35.95904029707086	38.0	35.0	40.0	30.0	41.0
49	35.918833635248056	39.0	35.0	40.0	30.0	41.0
50	35.836961639005544	38.0	35.0	40.0	30.0	41.0
51	35.389797450685734	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	3.0
15	3.0
16	21.0
17	54.0
18	93.0
19	201.0
20	403.0
21	751.0
22	1154.0
23	1905.0
24	3412.0
25	6538.0
26	10076.0
27	11897.0
28	11370.0
29	11072.0
30	12226.0
31	14909.0
32	18636.0
33	25202.0
34	45538.0
35	88546.0
36	93716.0
37	69683.0
38	119265.0
39	360083.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.614435235708484	19.727802652512874	25.164583032429277	28.49317907934936
2	33.49599609256562	19.801673449181852	26.576287033249574	20.126043425002948
3	21.184049641175363	22.14062133057843	37.67884726275622	18.99648176548999
4	22.457163207051025	23.8524392247315	35.54442225870435	18.145975309513126
5	19.82648080627218	29.31722435442988	30.86211629909514	19.994178540202803
6	71.3226952035802	1.5383869043615745	22.53158527832201	4.60733261373622
7	71.9643788403167	1.3093874346877417	18.269791585123965	8.456442139871598
8	58.573036718196136	3.0954068902709957	27.800447414466987	10.531108977065875
9	31.229595948793204	19.48524182762966	30.168171830088347	19.116990393488788
10	24.24097756422625	18.32139088787185	44.738910835743326	12.69872071215858
11	21.991446423275253	15.44031956286129	40.718906182985684	21.849327830877773
12	16.263482798578593	20.69308448062766	44.35224682989541	18.691185890898346
13	11.674893521310622	24.158286373925154	43.08034606814415	21.08647403662007
14	9.702653505169302	22.916374950523107	46.27972334835373	21.101248195953865
15	10.19703656824945	18.40297952896893	48.91834410340147	22.481639799380147
16	10.069912645026566	25.70769877032687	40.34492149298393	23.877467091662634
17	12.747012367294422	22.722987820138943	41.7396462360624	22.790353576504234
18	14.135342324989608	29.764859331259807	39.487358722101376	16.612439621649216
19	14.13776793323844	23.76313273920632	36.89295019013461	25.20614913742063
20	11.411494516471533	31.727948189007805	34.825449922766424	22.035107371754233
21	12.930917574524056	24.650133573836065	36.13263225904614	26.286316592593735
22	10.534967899279925	27.661967234442752	32.44978715287616	29.353277713401155
23	10.425595018241676	23.608555340803473	43.521806769431606	22.444042871523248
24	16.606375601027136	21.75770599202416	38.628803656935204	23.007114750013507
25	11.447658130363212	26.138905764016982	38.57047880404283	23.842957301576977
26	14.488378580115082	21.799602861776716	38.082821291107166	25.62919726700103
27	21.137632319686347	22.903364869915734	38.61843969441746	17.34056311598046
28	15.438445229214468	23.1272926132511	34.44231407437135	26.99194808316308
29	16.19291964952166	23.462467571271535	38.339163981040564	22.005448798166242
30	24.00856019201997	21.727826908595365	32.482312354394594	21.78130054499007
31	17.61190047508845	25.564146863964172	34.790388858078764	22.033563802868613
32	17.38642916286747	21.431902702237842	38.97776048000583	22.20390765488887
33	16.18762741334239	21.314811976771495	39.956383153489185	22.541177456396934
34	15.77395095199611	26.60914299952921	37.17674635524797	20.440159693226708
35	15.525767126172424	21.835435710907188	40.279650580106264	22.359146582814123
36	14.342511320423954	24.461266895188366	38.52902295397188	22.667198830415806
37	13.718137706190484	25.49997850029052	39.81547736521612	20.96640642830288
38	12.281405838439055	24.19389871321482	32.57922642942748	30.945469018918644
39	18.324037005961483	19.161753891171777	36.20826713444154	26.305941968425195
40	13.253964491300335	16.848274896387938	41.46852938679521	28.429231225516517
41	18.106834812770607	21.90335274187449	32.07414863906839	27.915663806286517
42	19.266496065553167	17.10428682156013	37.767381963838595	25.86183514904811
43	21.81294370714529	20.981842117159083	36.52447824615293	20.680735929542696
44	14.422776902476214	24.107128090858875	38.47091861092031	22.9991763957446
45	17.806279899756227	21.319552938348757	36.80761288174388	24.066554280151138
46	22.069286397078685	18.690193596614733	35.0495981759426	24.190921830363983
47	15.561820485143702	16.524125430407647	39.87071508033725	28.043339004111406
48	17.10031764442568	15.161264359325196	33.846386229601464	33.89203176664767
49	19.050506676486705	15.095442171845525	40.65694291772006	25.197108233947713
50	17.1550040849448	14.412412939958477	40.948567182181925	27.4840157929148
51	14.91837276968078	14.031041170289827	39.25505160481549	31.795534455213897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	1408.0
2	2620.0
3	34733.0
4	66846.0
5	36260.0
6	5674.0
7	5237.5
8	4801.0
9	5091.0
10	5381.0
11	5588.0
12	5795.0
13	5663.0
14	5531.0
15	5232.0
16	4933.0
17	4411.5
18	3890.0
19	3592.5
20	3295.0
21	3125.5
22	2956.0
23	2716.5
24	2477.0
25	2643.5
26	3292.0
27	3774.0
28	4192.5
29	4611.0
30	5263.5
31	5916.0
32	6972.5
33	8029.0
34	9738.0
35	11447.0
36	13179.0
37	14911.0
38	18491.0
39	22071.0
40	28017.5
41	33964.0
42	46597.0
43	59230.0
44	84086.0
45	108942.0
46	135892.0
47	162842.0
48	156438.0
49	150034.0
50	116126.0
51	82218.0
52	62636.0
53	43054.0
54	33144.0
55	23234.0
56	19489.0
57	15744.0
58	13933.0
59	12122.0
60	10219.5
61	8317.0
62	7034.0
63	5751.0
64	5006.5
65	4262.0
66	3443.5
67	2625.0
68	2196.0
69	1767.0
70	1556.0
71	1345.0
72	1362.0
73	1379.0
74	1166.0
75	757.0
76	561.0
77	479.5
78	398.0
79	288.0
80	178.0
81	122.0
82	66.0
83	45.5
84	25.0
85	15.5
86	6.0
87	3.5
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	906989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.871638990989815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9439885442012	20.051968762758833
2	12.13145966272388	6.762473283063901
3	4.936735557423403	4.1278473375146385
4	2.5418261283669867	2.8337944091083993
5	1.492319733094289	2.079669842996715
6	0.9733802825152824	1.627782230112818
7	0.6962768006044016	1.3584462936973094
8	0.47449413902173365	1.0579943476923435
9	0.3890955275683062	0.97602570696533
>10	3.6532052224376534	22.381882629356866
>50	0.49363974296969093	9.291250344359339
>100	0.25321002898164097	12.855115151729061
>500	0.012380932016452476	2.386930087272057
>1k	0.006390158460104505	3.024209141025381
>5k	0.0011981547112695945	2.258361125335797
>10k+	3.9938490375653155E-4	6.926249307011237
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62222	6.860281657219658	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	7540	0.83132209982701	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	7266	0.8011122516370098	TruSeq Adapter, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	5482	0.6044174736408049	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	3487	0.3844589074398918	TruSeq Adapter, Index 10 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	2681	0.29559344159631484	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	2505	0.27618857560565785	No Hit
ACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	2274	0.2507196889929205	TruSeq Adapter, Index 10 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1969	0.21709193827047515	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1705	0.18798463928448966	No Hit
AGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1658	0.18280265802562104	TruSeq Adapter, Index 10 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	1416	0.15612096728846767	Illumina PCR Primer Index 2 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1416	0.15612096728846767	TruSeq Adapter, Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1394	0.15369535903963555	TruSeq Adapter, Index 10 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1141	0.1258008641780661	RNA PCR Primer, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1140	0.12569060925766465	TruSeq Adapter, Index 10 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1117	0.12315474608843106	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	1115	0.12293423624762814	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1083	0.11940607879478142	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTT	1067	0.11764200006835804	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	943	0.10397038993857698	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	937	0.10330886041616821	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	929	0.10242682105295654	No Hit
ATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	907	0.10000121280412443	TruSeq Adapter, Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8191940585828494	0.0
2	0.0	0.0	0.0	2.7559319903548998	0.0
3	0.0	0.0	0.0	4.637983481607826	0.0
4	0.0	0.0	0.0	5.820467502913487	0.0
5	0.0	0.0	0.0	11.754277063999673	0.0
6	0.0	0.0	0.0	13.329378856854934	0.0
7	0.0	0.0	0.0	18.44774302665192	0.0
8	0.0	0.0	0.0	24.55377077340519	0.0
9	0.0	0.0	0.0	29.3490880264259	0.0
10	0.0	0.0	0.0	32.01979296331047	0.0
11	0.0	0.0	0.0	34.43448597502285	0.0
12	0.0	0.0	0.0	36.19613909319738	0.0
13	0.0	0.0	0.0	36.890193817124576	0.0
14	0.0	0.0	0.0	37.38137948751308	0.0
15	0.0	0.0	0.0	38.89187189701308	0.0
16	0.0	0.0	0.0	40.70997553443316	0.0
17	0.0	0.0	0.0	42.827090515982	0.0
18	1.1025492040146021E-4	0.0	0.0	43.80394911073894	0.0
19	1.1025492040146021E-4	0.0	0.0	44.939464535953576	0.0
20	1.1025492040146021E-4	0.0	0.0	46.039147112037746	0.0
21	1.1025492040146021E-4	0.0	0.0	47.09913791677738	0.0
22	3.3076476120438066E-4	0.0	0.0	48.147662209795264	0.0
23	3.3076476120438066E-4	0.0	0.0	48.934992596382095	0.0
24	0.0017640787264233634	0.0	0.0	49.59641186387046	0.0
25	0.0017640787264233634	0.0	0.0	50.18605517817746	0.0
26	0.0017640787264233634	0.0	0.0	50.78231378770856	0.0
27	0.0017640787264233634	0.0	0.0	51.37217761185637	0.0
28	0.0017640787264233634	0.0	0.0	51.938005863356665	0.0
29	0.0017640787264233634	0.0	0.0	52.64374760884641	0.0
30	0.0017640787264233634	0.0	0.0	53.23769086504908	0.0
31	0.0017640787264233634	0.0	0.0	53.74298916524897	0.0
32	0.0017640787264233634	0.0	0.0	54.24520032767762	0.0
33	0.0017640787264233634	0.0	0.0	54.73120401680726	0.0
34	0.0017640787264233634	0.0	0.0	55.309821839074125	0.0
35	0.0017640787264233634	0.0	0.0	55.75756707082445	0.0
36	0.0017640787264233634	0.0	0.0	56.182710043892484	0.0
37	0.0017640787264233634	0.0	0.0	56.63828337499132	0.0
38	0.0017640787264233634	0.0	0.0	57.08073637056238	0.0
39	0.0017640787264233634	0.0	0.0	57.52329962105384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.032498E-4	45.000004	1
CCTTCGC	40	6.8121153E-9	45.000004	42
CTTCGCG	40	6.8121153E-9	45.000004	43
AACAATG	20	7.032498E-4	45.000004	20
GGTACTA	70	0.0	45.000004	7
GATCGGA	20	7.032498E-4	45.000004	8
CGGCCTA	20	7.032498E-4	45.000004	5
GGCACGG	75	0.0	45.000004	1
AGCTTAC	20	7.032498E-4	45.000004	10
CGTTGAA	20	7.032498E-4	45.000004	11
ATTACGG	75	0.0	45.000004	1
CCGGAGG	20	7.032498E-4	45.000004	1
CGCGCAG	30	2.164994E-6	45.000004	34
TGAACCG	20	7.032498E-4	45.000004	12
CTAAGTG	20	7.032498E-4	45.000004	23
CACTAGG	35	1.2115561E-7	45.000004	1
GAGCGAT	20	7.032498E-4	45.000004	18
CGAGGGT	40	6.8121153E-9	45.000004	3
TCTCGCG	30	2.164994E-6	45.000004	31
GAACGTA	20	7.032498E-4	45.000004	7
>>END_MODULE