FastQCFastQC Report
Thu 26 May 2016
SRR1043264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043264_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2139600
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT856864.004767246214246No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC153380.7168629650401944No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA103230.4824733595064498No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT53010.24775659001682557No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT42550.19886894746681621Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC37070.17325668349224152No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35290.16493737147130305No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30820.1440456160029912No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT29340.13712843522153673No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC28140.13151991026360066No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT25800.12058328659562535No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25460.11899420452421014No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25280.11815292578051972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA351.02012564E-746.00000415
CGCGAAA253.4181496E-546.046
TACTCGA253.4181496E-546.039
CACGTCG253.4181496E-546.032
CGTTTTT235300.045.2668951
ATTGCGG4350.044.413791
CTAGCGG5450.043.8899081
TCCGTAC1450.042.82758746
TAGTAGG4100.042.6341441
GCGCGAC18050.041.7950138
TACGGGA4200.041.6190453
TCGGCGT14900.041.369134
CTTACGG2450.041.306121
TAGCGGG15850.041.2113572
GTCGGCG14950.041.0769233
CCACCCG13750.040.9818245
ACCCGCA38500.040.92207731
CGACGGT3600.040.8888927
CGCACTT38450.040.85565634
CCCGCAC38750.040.8361332
Produced by FastQC (version 0.11.5)