FastQCFastQC Report
Thu 26 May 2016
SRR1043263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043263_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2099488
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT823013.920050983858922No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC148940.7094110564099438No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG87740.4179114145925102No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC52400.2495846606410706No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT42220.20109664832568702No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG36700.17480452376960479No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC34610.1648497157402186No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29860.1422251520370681No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC29380.1399388803365392No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC27780.13231797466810954No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT27030.12874567513603316No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC25070.11941006569220686No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC23860.11364675578045694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT223750.044.296091
GGTACGA603.6379788E-1241.2500047
CACGACG3350.040.9701525
CTAGCGG5550.040.9459461
ATTGCGG4950.040.909091
CGTAGGG8150.040.582822
TAGCGGG15500.040.52
GCGATAG1500.040.58
CGCACTT37100.040.4514834
GCGCGAC17000.040.367658
TACGGGA4300.040.29073
ACCCGCA37550.040.20639431
CCCGCAC37650.040.15936332
GTCGGCG13000.040.1538433
TCGGCGT12850.040.0972754
TACGCGG2750.040.0909081
CCGCACT37300.040.0536233
GCGTAGG3150.040.01
GCACTTA37550.039.9067935
TCACGAC3500.039.85714324