##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043263_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2099488
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45995833269826	33.0	31.0	34.0	31.0	34.0
2	32.75280115913975	34.0	31.0	34.0	31.0	34.0
3	32.855337587068846	34.0	31.0	34.0	31.0	34.0
4	36.303210592296786	37.0	37.0	37.0	35.0	37.0
5	36.292663735158285	37.0	37.0	37.0	35.0	37.0
6	36.42239345973876	37.0	37.0	37.0	35.0	37.0
7	36.3945452415065	37.0	37.0	37.0	35.0	37.0
8	36.3187924865491	37.0	37.0	37.0	35.0	37.0
9	37.879206739928975	39.0	38.0	39.0	35.0	39.0
10	37.7137945060891	39.0	37.0	39.0	35.0	39.0
11	37.389435424255815	39.0	37.0	39.0	35.0	39.0
12	37.376730421893335	39.0	37.0	39.0	35.0	39.0
13	37.37696762258227	39.0	37.0	39.0	35.0	39.0
14	38.74606094438263	40.0	38.0	41.0	35.0	41.0
15	38.801546377021445	41.0	38.0	41.0	35.0	41.0
16	38.708303643555	41.0	38.0	41.0	34.0	41.0
17	38.695001352710754	40.0	38.0	41.0	34.0	41.0
18	38.53590113399076	40.0	38.0	41.0	34.0	41.0
19	38.341845249889495	40.0	37.0	41.0	35.0	41.0
20	38.117266685972965	40.0	35.0	41.0	35.0	41.0
21	38.05251994772059	40.0	35.0	41.0	35.0	41.0
22	38.016043911658464	40.0	35.0	41.0	34.0	41.0
23	37.99483874163606	40.0	35.0	41.0	35.0	41.0
24	37.91348128686613	40.0	35.0	41.0	34.0	41.0
25	37.89818088981695	40.0	35.0	41.0	34.0	41.0
26	37.857837720434695	40.0	35.0	41.0	34.0	41.0
27	37.85564099437577	40.0	35.0	41.0	34.0	41.0
28	37.77052309896508	40.0	35.0	41.0	34.0	41.0
29	37.69940480726729	40.0	35.0	41.0	34.0	41.0
30	37.6027817258303	40.0	35.0	41.0	34.0	41.0
31	37.4994208111692	40.0	35.0	41.0	34.0	41.0
32	37.24564417610389	40.0	35.0	41.0	33.0	41.0
33	36.88912058559039	40.0	35.0	41.0	33.0	41.0
34	36.57089014083434	40.0	35.0	41.0	31.0	41.0
35	36.408924937889616	39.0	35.0	41.0	31.0	41.0
36	36.34702889466384	39.0	35.0	41.0	31.0	41.0
37	36.28083323172126	39.0	35.0	41.0	31.0	41.0
38	36.21465709734945	39.0	35.0	41.0	31.0	41.0
39	36.161488896340444	39.0	35.0	41.0	30.0	41.0
40	36.086494897803654	39.0	35.0	41.0	30.0	41.0
41	36.011523761983874	39.0	35.0	41.0	30.0	41.0
42	35.89750072398604	39.0	35.0	41.0	30.0	41.0
43	35.7843021727202	38.0	35.0	41.0	30.0	41.0
44	35.67002812114192	38.0	35.0	41.0	29.0	41.0
45	35.627807827432214	38.0	35.0	41.0	29.0	41.0
46	35.549360129707814	38.0	35.0	40.0	29.0	41.0
47	35.42788098812663	38.0	35.0	40.0	28.0	41.0
48	35.36500089545642	38.0	35.0	40.0	28.0	41.0
49	35.293387244890184	37.0	35.0	40.0	28.0	41.0
50	35.22994606304013	37.0	35.0	40.0	27.0	41.0
51	34.829841847155116	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	10.0
15	18.0
16	49.0
17	131.0
18	256.0
19	578.0
20	1106.0
21	1843.0
22	2971.0
23	4684.0
24	8223.0
25	15762.0
26	25935.0
27	33484.0
28	35147.0
29	33536.0
30	33328.0
31	36283.0
32	42220.0
33	56229.0
34	141667.0
35	199246.0
36	160693.0
37	160615.0
38	303696.0
39	801598.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.603206115014707	17.80572215702114	25.08521125150513	29.505860476459024
2	33.3647536923288	19.888134630919538	24.386278940389275	22.36083273636239
3	25.048059336371537	21.22865193799631	34.09521750064778	19.628071224984378
4	22.92025484308555	23.951744425307503	31.942359279976834	21.18564145163011
5	21.318483363562926	31.68143852215397	28.54743632733314	18.45264178694996
6	80.52477556432807	1.680838375832584	15.396372829947111	2.3980132298922405
7	80.92272973220138	1.4263477571674616	14.031659147373073	3.61926336325809
8	73.49206092151991	2.504515386608545	18.412108094926	5.591315596945541
9	37.91781615327166	17.98805232513832	23.79256275815818	20.301568763431845
10	32.04728962489902	22.87591069822738	30.769835312228505	14.306964364645095
11	29.449465774512646	18.084694935146093	33.58923699492448	18.876602295416784
12	23.70982830099529	18.37447987318813	36.46922487768446	21.446466948132116
13	19.314947263332773	21.304479949397187	37.92886646649088	21.451706320779163
14	19.76277073267387	21.263088905485528	38.760878842841684	20.21326151899892
15	22.873148119922572	17.414341020286848	39.46662233839869	20.245888521391883
16	20.70585780914204	20.487566492401957	37.440032998521545	21.36654269993446
17	20.766444009206054	22.27333521315673	37.482233763660474	19.47798701397674
18	21.553016735508848	27.46679190354982	33.39256999801856	17.58762136292277
19	22.642425200810866	23.487869423402277	32.91769231355454	20.952013062232318
20	21.449181895776494	27.24307069152098	33.9080766358274	17.399670776875123
21	18.586864988035178	21.346109146610985	35.70980162782545	24.35722423752839
22	20.07741887545916	23.64295485375482	33.76323179746681	22.51639447331921
23	20.892569998018566	21.768354951302413	37.41459822585316	19.924476824825863
24	23.09062971543538	20.562965827858985	34.10321945159963	22.243185005106007
25	18.146186117758234	21.829226935328993	36.70290089774269	23.321686049170083
26	17.470640460912374	20.976114176408725	36.27289129540154	25.280354067277354
27	21.097572360499324	23.164409608437868	36.07708165038333	19.66093638067948
28	23.26433873401515	22.797986937767682	32.87472945784877	21.062944870368394
29	22.07604901766526	20.51428729290189	37.56963602554528	19.84002766388758
30	26.74756893109177	20.15210375100977	32.78723193464311	20.313095383255348
31	27.28465225807435	19.45707715404899	32.640338977884134	20.61793160999253
32	25.51584005243183	19.223020088707344	33.23438857473822	22.026751284122607
33	24.16522504534439	19.828358152082792	35.49036717523511	20.51604962733771
34	25.08025766282065	20.550629486808212	34.13956164550595	20.22955120486519
35	25.236248075721317	23.411707997378407	31.235758432532123	20.11628549436815
36	25.57895067749851	21.7920750201954	32.620715145787926	20.00825915651816
37	26.153471703577253	23.62075896599552	30.339206511301803	19.886562819125427
38	23.54083471779786	20.843081741834197	30.53596876952857	25.08011477083937
39	23.094487798930025	19.08293831638952	34.929373256717824	22.893200627962624
40	19.798112682711213	20.10009106982274	37.1492001859501	22.95259606151595
41	21.157825145940343	23.582940221615935	31.400989193555763	23.858245438887955
42	20.511905759880506	19.480606700300264	33.34441540032618	26.663072139493053
43	23.26619632977183	20.301378240790136	33.26434826014724	23.1680771692908
44	22.811704567970857	21.539727781249525	33.8344396348062	21.81412801597342
45	23.57898687680044	21.40793374384612	32.568369049977896	22.444710329375543
46	23.008895502141474	21.11205208126934	32.18732376655642	23.69172865003277
47	20.737246414363884	21.869903519334237	34.62753776158758	22.765312304714293
48	22.725731225898883	20.081372220274655	32.02399823194988	25.168898321876572
49	22.048613757258913	18.021727202060692	35.11156053285372	24.81809850782667
50	20.51523990611044	18.95824124738984	36.68046685668125	23.84605198981847
51	19.312470468990533	17.319413114054473	36.515569510280606	26.85254690667439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	2489.0
2	4580.0
3	59595.5
4	114611.0
5	66507.5
6	18404.0
7	16724.0
8	15044.0
9	14588.0
10	14132.0
11	13579.5
12	13027.0
13	12207.0
14	11387.0
15	10614.0
16	9841.0
17	9205.5
18	8570.0
19	7916.5
20	7263.0
21	7044.5
22	6826.0
23	6645.0
24	6464.0
25	6777.0
26	8497.0
27	9904.0
28	11010.5
29	12117.0
30	13556.0
31	14995.0
32	17992.5
33	20990.0
34	25079.0
35	29168.0
36	32464.5
37	35761.0
38	40237.5
39	44714.0
40	49938.5
41	55163.0
42	64543.0
43	73923.0
44	83064.0
45	92205.0
46	103251.0
47	114297.0
48	143124.0
49	171951.0
50	198322.0
51	224693.0
52	223923.0
53	223153.0
54	199143.5
55	175134.0
56	153579.5
57	132025.0
58	117741.5
59	103458.0
60	96268.0
61	89078.0
62	81722.0
63	74366.0
64	69733.5
65	65101.0
66	50893.0
67	36685.0
68	28258.0
69	19831.0
70	16657.5
71	13484.0
72	13409.5
73	13335.0
74	11113.0
75	6450.5
76	4010.0
77	2951.5
78	1893.0
79	1352.5
80	812.0
81	572.0
82	332.0
83	271.0
84	210.0
85	159.0
86	108.0
87	71.0
88	34.0
89	24.5
90	15.0
91	12.0
92	9.0
93	6.0
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2099488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.855603864922273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64236430286981	20.792081315619562
2	10.05731833955649	5.603053512202068
3	3.5219816775405284	2.9432077928725007
4	1.7949791908905606	2.000009171489046
5	1.0965949642951618	1.5273157462837308
6	0.7677201705873773	1.2831185370595521
7	0.599116690151186	1.168213003280037
8	0.49748163889165575	1.1086121170430618
9	0.43453821844772444	1.0893892029523984
>10	5.846245583002481	37.12323982407999
>50	0.6051064659591641	10.92322265394816
>100	0.12600891368188355	5.7832390879093625
>500	0.006222596533468579	1.178142819294233
>1k	0.0036298479778566705	2.1226215288154915
>5k	3.45699807414921E-4	0.6747514597895042
>10k+	3.45699807414921E-4	4.6797822273612715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82301	3.920050983858922	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	14894	0.7094110564099438	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8774	0.4179114145925102	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	5240	0.2495846606410706	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	4222	0.20109664832568702	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	3670	0.17480452376960479	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3461	0.1648497157402186	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2986	0.1422251520370681	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	2938	0.1399388803365392	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2778	0.13231797466810954	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	2703	0.12874567513603316	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2507	0.11941006569220686	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2386	0.11364675578045694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20752678748342454	0.0
2	0.0	0.0	0.0	0.6943121370543676	0.0
3	0.0	0.0	0.0	1.219916474873874	0.0
4	0.0	0.0	0.0	1.5651435016537365	0.0
5	0.0	0.0	0.0	3.4529370970446127	0.0
6	0.0	0.0	0.0	4.169111707235288	0.0
7	0.0	0.0	0.0	6.211704949016141	0.0
8	0.0	0.0	0.0	8.421767592860736	0.0
9	0.0	0.0	0.0	10.904753920955967	0.0
10	0.0	0.0	0.0	12.163822798701398	0.0
11	0.0	0.0	0.0	13.014792177902422	0.0
12	0.0	0.0	0.0	13.68543187672423	0.0
13	0.0	0.0	0.0	14.042185523327593	0.0
14	0.0	0.0	0.0	14.283434818393818	0.0
15	0.0	0.0	0.0	14.911254553491137	0.0
16	0.0	0.0	0.0	15.559603103232789	0.0
17	0.0	0.0	0.0	16.34231774604094	0.0
18	0.0	0.0	0.0	16.793237208309836	0.0
19	0.0	0.0	0.0	17.30279001356521	0.0
20	0.0	0.0	0.0	17.89093340852627	0.0
21	0.0	0.0	0.0	18.460786629883096	0.0
22	0.0	0.0	0.0	19.066362846560686	0.0
23	0.0	0.0	0.0	19.581059763142253	0.0
24	0.0	0.0	0.0	20.024977518328278	0.0
25	0.0	0.0	0.0	20.434886981968937	0.0
26	0.0	0.0	0.0	20.8630389885534	0.0
27	0.0	0.0	0.0	21.319721760734044	0.0
28	0.0	0.0	0.0	21.774499306497585	0.0
29	0.0	0.0	0.0	22.257474203234313	0.0
30	0.0	0.0	0.0	22.68576910180006	0.0
31	0.0	0.0	0.0	23.122875672544925	0.0
32	0.0	0.0	0.0	23.585226493316465	0.0
33	0.0	0.0	0.0	24.024190659818014	0.0
34	0.0	0.0	0.0	24.506403465987898	0.0
35	4.7630660427685226E-5	0.0	0.0	24.93827066408572	0.0
36	4.7630660427685226E-5	0.0	0.0	25.39981176362999	0.0
37	4.7630660427685226E-5	0.0	0.0	25.997624182657866	0.0
38	4.7630660427685226E-5	0.0	0.0	26.576479598835526	0.0
39	4.7630660427685226E-5	0.0	0.0	27.20934818393818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22375	0.0	44.29609	1
GGTACGA	60	3.6379788E-12	41.250004	7
CACGACG	335	0.0	40.97015	25
CTAGCGG	555	0.0	40.945946	1
ATTGCGG	495	0.0	40.90909	1
CGTAGGG	815	0.0	40.58282	2
TAGCGGG	1550	0.0	40.5	2
GCGATAG	150	0.0	40.5	8
CGCACTT	3710	0.0	40.45148	34
GCGCGAC	1700	0.0	40.36765	8
TACGGGA	430	0.0	40.2907	3
ACCCGCA	3755	0.0	40.206394	31
CCCGCAC	3765	0.0	40.159363	32
GTCGGCG	1300	0.0	40.153843	3
TCGGCGT	1285	0.0	40.097275	4
TACGCGG	275	0.0	40.090908	1
CCGCACT	3730	0.0	40.05362	33
GCGTAGG	315	0.0	40.0	1
GCACTTA	3755	0.0	39.90679	35
TCACGAC	350	0.0	39.857143	24
>>END_MODULE