FastQCFastQC Report
Thu 26 May 2016
SRR1043262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043262_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2190077
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1382706.313476649451139No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT115760.5285658906056727TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT94210.4301675237902594TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC77090.35199675627843224No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC75810.346152212913062TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT64110.2927294337139745No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC55100.25158932768117287No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT53640.24492289540504739TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA50250.22944398758582463No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG49740.2271153023386849No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT43970.2007691966994768No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG33240.1517754855194589TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT31920.14574830017392082No Hit
AGCCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT25800.11780407720824426No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT25770.11766709572311841TruSeq Adapter, Index 15 (95% over 22bp)
GGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT25670.11721049077269886No Hit
AGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT24630.11246179928833552No Hit
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC24050.10981349057590213TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA206.3127134E-446.010
ACCCACG206.3127134E-446.022
CGTTTTT327850.045.4668271
AATGCGG2800.042.7142871
GCGATAT650.042.461548
GCGTAGG2850.041.964911
ATTGCGG3750.041.7066651
CGGTCTA3100.041.5483930
TATGCGG3550.041.4647871
GTTTTTT362900.041.348032
ACCCGCA21000.041.18095431
TCAACGC24400.041.0983615
TCACGAC3250.041.04615424
ACGGGTA1850.041.0270274
CACGACG3150.040.88889325
ATATCGG900.040.888891
TCCGTAC451.5896148E-840.8888946
CAACGCA24150.040.85714316
TATGACC22600.040.80973427
ACGTAGG1750.040.742861
Produced by FastQC (version 0.11.5)