##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043262_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2190077
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.327770210819075	33.0	31.0	34.0	31.0	34.0
2	32.661556648464874	34.0	31.0	34.0	31.0	34.0
3	32.74058537667854	34.0	31.0	34.0	31.0	34.0
4	36.21636910483056	37.0	37.0	37.0	35.0	37.0
5	36.14412826580983	37.0	37.0	37.0	35.0	37.0
6	36.325998583611444	37.0	37.0	37.0	35.0	37.0
7	36.25688822813079	37.0	37.0	37.0	35.0	37.0
8	36.23453193654835	37.0	37.0	37.0	35.0	37.0
9	37.8481843332449	39.0	38.0	39.0	35.0	39.0
10	37.692773359110205	39.0	37.0	39.0	35.0	39.0
11	37.40122972845247	39.0	37.0	39.0	35.0	39.0
12	37.401074482769324	39.0	37.0	39.0	35.0	39.0
13	37.37799492894542	39.0	37.0	39.0	35.0	39.0
14	38.63161477884111	40.0	38.0	41.0	34.0	41.0
15	38.732779715051116	41.0	38.0	41.0	35.0	41.0
16	38.68779271231103	41.0	38.0	41.0	34.0	41.0
17	38.60117657963624	40.0	38.0	41.0	34.0	41.0
18	38.35503317919872	40.0	38.0	41.0	34.0	41.0
19	38.08174324464391	40.0	37.0	41.0	34.0	41.0
20	37.645580041249694	39.0	35.0	41.0	34.0	41.0
21	37.630765037028375	39.0	35.0	41.0	34.0	41.0
22	37.605359994191986	39.0	35.0	41.0	34.0	41.0
23	37.596404601299405	39.0	35.0	41.0	34.0	41.0
24	37.52852114332053	39.0	35.0	41.0	34.0	41.0
25	37.41581049433422	39.0	35.0	41.0	34.0	41.0
26	37.284160785214404	39.0	35.0	41.0	33.0	41.0
27	37.29259793148825	39.0	35.0	41.0	33.0	41.0
28	37.284570359854925	39.0	35.0	41.0	33.0	41.0
29	37.118089455302254	39.0	35.0	41.0	33.0	41.0
30	37.07059203854477	39.0	35.0	41.0	33.0	41.0
31	36.829624711825204	39.0	35.0	41.0	33.0	41.0
32	36.472875611222804	38.0	35.0	41.0	31.0	41.0
33	35.99040946962139	38.0	35.0	41.0	30.0	41.0
34	35.82349068092127	38.0	35.0	41.0	29.0	41.0
35	35.63467494521882	38.0	35.0	41.0	28.0	41.0
36	35.64674712350296	38.0	35.0	41.0	29.0	41.0
37	35.57912666997553	38.0	35.0	41.0	29.0	41.0
38	35.46271797749577	38.0	35.0	41.0	28.0	41.0
39	35.41822091186748	38.0	35.0	41.0	27.0	41.0
40	35.390049756241446	38.0	35.0	40.0	27.0	41.0
41	35.34549835462406	38.0	35.0	40.0	27.0	41.0
42	35.290745485204404	38.0	35.0	40.0	27.0	41.0
43	35.20005232692732	38.0	35.0	40.0	26.0	41.0
44	35.011491376787205	37.0	35.0	40.0	25.0	41.0
45	34.915836292513916	37.0	35.0	40.0	24.0	41.0
46	34.886253314381186	37.0	35.0	40.0	24.0	41.0
47	34.8105199040947	37.0	35.0	40.0	24.0	41.0
48	34.734794712697315	37.0	35.0	40.0	23.0	41.0
49	34.68255454031981	36.0	35.0	40.0	24.0	41.0
50	34.58104441076729	36.0	35.0	40.0	24.0	41.0
51	34.4718929973695	36.0	34.0	40.0	24.0	41.0
52	34.12083776049883	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	4.0
13	10.0
14	12.0
15	40.0
16	69.0
17	181.0
18	365.0
19	885.0
20	1592.0
21	2716.0
22	4311.0
23	7108.0
24	12991.0
25	23800.0
26	35138.0
27	40938.0
28	41940.0
29	40424.0
30	41793.0
31	45971.0
32	53894.0
33	68765.0
34	143602.0
35	195370.0
36	233244.0
37	201405.0
38	302389.0
39	690261.0
40	857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.480312336050282	16.453302783418117	22.545463013400898	33.520921867130696
2	38.671380047368196	18.034069121770603	22.346748538978307	20.947802291882887
3	23.80359229378693	19.85158512691563	36.951349199137745	19.393473380159694
4	22.204379115437494	22.1714122380172	35.22451493714605	20.39969370939926
5	20.856024696848557	28.82400938414494	32.33530145287129	17.984664466135207
6	72.56845307265452	2.120336408263271	21.820283031144566	3.490927487937639
7	72.01125805165755	1.9583786323494563	20.264173360114736	5.766189955878263
8	61.81047515680955	3.44074660388653	26.51121398927983	8.237564250024086
9	38.18692219497306	13.617375096857327	30.124237640959656	18.071465067209967
10	25.73882105515012	14.738066287167072	39.42998351199524	20.09312914568757
11	22.878465003741876	12.320206093210421	42.10746928076045	22.693859622287253
12	20.70612129162582	13.220402752962567	44.82874346427089	21.244732491140724
13	17.086339886679784	15.881542064502755	46.01879294654937	21.013325102268094
14	14.766467115083168	16.712882697731633	47.292583776734794	21.22806641045041
15	17.9750757621764	16.083955039023742	45.42954425803294	20.511424940766922
16	19.07686350753878	19.178686411482335	41.05613638241943	20.68831369855946
17	17.9816052129674	18.960292263696665	42.45754829624712	20.600554227088818
18	19.594379558344297	24.23512963242845	38.60279798381518	17.567692825412074
19	20.508685311064408	20.62411504253047	36.689760223042384	22.177439423362742
20	18.306799258656202	25.34632343976947	37.69050129287692	18.65637600869741
21	18.80308317926721	20.049614693912588	37.57443231448027	23.57286981233993
22	19.351191761750844	21.609970790981322	35.130454317359614	23.908383129908216
23	18.088953036811034	19.421919868570832	39.08816904611116	23.400958048506972
24	22.42523892995543	17.83832257952574	36.44917507466632	23.287263415852504
25	16.94639046937619	19.497761950835518	38.567822044613045	24.988025535175247
26	16.53014939657373	19.80510274296292	37.80853367256037	25.85621418790298
27	20.237781593980486	19.95372765432448	36.988471181606855	22.820019570088174
28	20.780045633098744	20.57210773867768	33.57649068959676	25.071355938626816
29	19.765789056731794	20.589230424318412	34.91210583006899	24.732874688880802
30	22.089360328426807	18.40592819339229	32.28461830337472	27.22009317480618
31	23.381232714648846	19.299549741858392	31.579346296956682	25.73987124653608
32	21.398791001412278	19.69159075228862	33.77310478124741	25.136513465051685
33	17.05739113282318	19.28037233394077	35.87828190515676	27.78395462807929
34	19.270966271962127	20.118105436475524	34.70649662089507	25.90443167066729
35	19.49461137667762	20.327869750698262	35.58441095906674	24.593107913557375
36	21.833844198172027	19.103026971197817	34.756768825936255	24.3063600046939
37	20.574847368380198	21.2814891896495	36.06539861383869	22.078264828131612
38	19.797842724251247	18.06689901770577	37.67483974307753	24.460418514965458
39	20.9667513973253	17.417058852268667	41.23439495506322	20.381794795342813
40	17.95151494673475	17.367334573167977	41.07695756815856	23.60419291193871
41	20.29024550278369	20.834472943188757	34.236147861467884	24.639133692559668
42	18.872532792226025	16.762606976832323	37.17695770514005	27.187902525801604
43	21.276420874699838	18.376431513595186	36.96742169339252	23.37972591831246
44	20.453070828103304	19.35142006422605	36.07224768809498	24.12326141957566
45	20.63064449332147	19.94336272194996	34.73745443653351	24.688538348195063
46	21.66700074928872	18.72705845502236	35.039452950741	24.56648784494792
47	19.48027398123445	18.204839373227514	36.89893095082958	25.415955694708455
48	20.528501965912614	16.434034054510413	34.322628839077346	28.714835140499627
49	20.088426114698247	16.200982887816274	38.35216752652989	25.35842347095559
50	20.31353235525509	15.089515117505	39.01401640216303	25.582936125076884
51	18.249677979358715	14.551040899475224	38.804206427445244	28.39507469372081
52	15.673649830576734	15.203118429169384	43.03410336714188	26.089128373111997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	589.0
1	3290.5
2	5992.0
3	89562.0
4	173132.0
5	97236.0
6	21340.0
7	19493.5
8	17647.0
9	17344.5
10	17042.0
11	16719.5
12	16397.0
13	15704.0
14	14061.0
15	13111.0
16	12457.5
17	11804.0
18	11040.5
19	10277.0
20	9692.5
21	9108.0
22	8599.0
23	8090.0
24	8032.5
25	7975.0
26	8703.0
27	9431.0
28	9499.0
29	9567.0
30	10286.5
31	11006.0
32	12225.5
33	13445.0
34	15549.5
35	17654.0
36	20212.5
37	22771.0
38	25643.5
39	30944.5
40	33373.0
41	39481.0
42	45589.0
43	58600.5
44	71612.0
45	94542.0
46	117472.0
47	156454.5
48	195437.0
49	220071.5
50	244706.0
51	232267.5
52	219829.0
53	189712.0
54	159595.0
55	142042.5
56	124490.0
57	112028.0
58	99566.0
59	94921.0
60	90276.0
61	87546.5
62	84817.0
63	83819.5
64	73207.5
65	63593.0
66	52804.5
67	42016.0
68	33330.0
69	24644.0
70	20444.5
71	16245.0
72	15355.0
73	14465.0
74	11953.5
75	9442.0
76	7197.0
77	4952.0
78	3903.5
79	2855.0
80	2069.5
81	1284.0
82	947.0
83	610.0
84	405.0
85	200.0
86	182.0
87	164.0
88	104.0
89	41.0
90	38.0
91	32.0
92	26.0
93	16.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2190077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.24659172192264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63985002916935	23.322409200469906
2	10.508767010191242	6.5672630453651095
3	3.803327839060075	3.565230965151975
4	1.9404803005348612	2.4253358278098625
5	1.2296348269820065	1.9210948702881876
6	0.8738430767037939	1.6382770708075307
7	0.6959154678883234	1.5221490548654044
8	0.5969775484771487	1.4922810979535799
9	0.5098038825033236	1.4336670397349138
>10	4.796456515366064	29.020213597111937
>50	0.23435166697982637	5.061694486906867
>100	0.15914104627902514	8.99744830356148
>500	0.005284979937730503	1.1090366683287782
>1k	0.004844564942919628	2.893302676848631
>5k	0.0010276349878920424	2.156926549261177
>10k+	2.936099965405835E-4	6.873669545534768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	138270	6.313476649451139	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	11576	0.5285658906056727	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCTT	9421	0.4301675237902594	TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7709	0.35199675627843224	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	7581	0.346152212913062	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	6411	0.2927294337139745	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	5510	0.25158932768117287	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5364	0.24492289540504739	TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5025	0.22944398758582463	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	4974	0.2271153023386849	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	4397	0.2007691966994768	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	3324	0.1517754855194589	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	3192	0.14574830017392082	No Hit
AGCCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2580	0.11780407720824426	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	2577	0.11766709572311841	TruSeq Adapter, Index 15 (95% over 22bp)
GGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2567	0.11721049077269886	No Hit
AGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2463	0.11246179928833552	No Hit
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2405	0.10981349057590213	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4439113327978879	0.0
2	0.0	0.0	0.0	1.4327806739215105	0.0
3	0.0	0.0	0.0	2.39215333524803	0.0
4	0.0	0.0	0.0	3.059207507315953	0.0
5	0.0	0.0	0.0	6.134715811361883	0.0
6	0.0	0.0	0.0	7.443117296789108	0.0
7	0.0	0.0	0.0	10.68122262367944	0.0
8	0.0	0.0	0.0	14.341778850697944	0.0
9	0.0	0.0	0.0	17.614586153820163	0.0
10	0.0	0.0	0.0	19.462694690643296	0.0
11	0.0	0.0	0.0	21.058894276319965	0.0
12	0.0	0.0	0.0	22.13333138515221	0.0
13	0.0	0.0	0.0	22.684773183773903	0.0
14	0.0	0.0	0.0	23.07836665103556	0.0
15	0.0	0.0	0.0	24.000617329892968	0.0
16	0.0	0.0	0.0	24.905380039149307	0.0
17	0.0	0.0	0.0	26.068763792323285	0.0
18	0.0	0.0	0.0	26.739105520034226	0.0
19	0.0	0.0	0.0	27.503325225551432	0.0
20	0.0	0.0	0.0	28.351377599965662	0.0
21	0.0	0.0	0.0	29.217922474871887	0.0
22	9.13209900839103E-5	0.0	0.0	29.95944891435324	0.0
23	1.3698148512586545E-4	0.0	0.0	30.675131513640846	0.0
24	4.109444553775963E-4	0.0	0.0	31.246846572061166	0.0
25	4.566049504195515E-4	0.0	0.0	31.820114087312913	0.0
26	4.566049504195515E-4	0.0	0.0	32.35557471266992	0.0
27	4.566049504195515E-4	0.0	0.0	32.88414060327559	0.0
28	4.566049504195515E-4	0.0	0.0	33.41129101853497	0.0
29	4.566049504195515E-4	0.0	0.0	33.96583773081951	0.0
30	4.566049504195515E-4	0.0	0.0	34.4988783499393	0.0
31	4.566049504195515E-4	0.0	0.0	35.021508376189516	0.0
32	4.566049504195515E-4	0.0	0.0	35.57655735391952	0.0
33	4.566049504195515E-4	0.0	0.0	36.09982662710033	0.0
34	4.566049504195515E-4	0.0	0.0	36.668665074333006	0.0
35	4.566049504195515E-4	0.0	0.0	37.16855617405233	0.0
36	4.566049504195515E-4	0.0	0.0	37.72360515178234	0.0
37	4.566049504195515E-4	0.0	0.0	38.344131279402504	0.0
38	4.566049504195515E-4	0.0	0.0	39.067165218391864	0.0
39	5.479259405034618E-4	0.0	0.0	39.755679823129505	0.0
40	6.39246930587372E-4	0.0	0.0	40.379767469362946	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGTA	20	6.3127134E-4	46.0	10
ACCCACG	20	6.3127134E-4	46.0	22
CGTTTTT	32785	0.0	45.466827	1
AATGCGG	280	0.0	42.714287	1
GCGATAT	65	0.0	42.46154	8
GCGTAGG	285	0.0	41.96491	1
ATTGCGG	375	0.0	41.706665	1
CGGTCTA	310	0.0	41.54839	30
TATGCGG	355	0.0	41.464787	1
GTTTTTT	36290	0.0	41.34803	2
ACCCGCA	2100	0.0	41.180954	31
TCAACGC	2440	0.0	41.09836	15
TCACGAC	325	0.0	41.046154	24
ACGGGTA	185	0.0	41.027027	4
CACGACG	315	0.0	40.888893	25
ATATCGG	90	0.0	40.88889	1
TCCGTAC	45	1.5896148E-8	40.88889	46
CAACGCA	2415	0.0	40.857143	16
TATGACC	2260	0.0	40.809734	27
ACGTAGG	175	0.0	40.74286	1
>>END_MODULE