FastQCFastQC Report
Thu 26 May 2016
SRR1043261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043261_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2142083
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1304006.087532555928038No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC106960.49932705688808515TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT93570.43681780771333323TruSeq Adapter, Index 14 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC74810.3492395019240617No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG74120.3460183382249894TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC60060.28038129241490645No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC53150.2481229718923123TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT50270.23467811471357555No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC45360.21175650056510414No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG40950.19116906301016345No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT40400.1886014687572797No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT30590.1428049239922076No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC30210.14103094978112427No Hit
AGCCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC26530.12385141005273839No Hit
GGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC25690.11992999337560684No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC25460.11885627214258271TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG24860.11605526023034589No Hit
AGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC23840.11129353997954326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT207.0346514E-445.00000416
CGTTTTT310100.044.2454071
TACGCGG2450.044.0816351
TCATCGG850.042.3529431
TATACGG750.042.0000041
TACGGGT1300.041.538463
ACGGGTA1250.041.44
CATGCGG2700.040.8333321
CGGTCTA2650.040.7547230
GTTTTTT341600.040.4091032
CACGACG2700.040.025
ATTGCGG4050.040.01
CGTTCAG4750.039.78947438
ACCCGCA19600.039.71938731
TCGATGG2550.039.7058831
TACTATT2950.039.6610245
GCGCGAC11800.039.470348
GCATAGG5250.039.4285741
CAACCCG1200.039.37500422
GCGATCG800.039.3750048
Produced by FastQC (version 0.11.5)