##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043261_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2142083
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.289369272805956	33.0	31.0	34.0	31.0	34.0
2	32.59110034485125	34.0	31.0	34.0	31.0	34.0
3	32.65414038578337	34.0	31.0	34.0	31.0	34.0
4	36.18248732658819	37.0	37.0	37.0	35.0	37.0
5	36.158933617418185	37.0	37.0	37.0	35.0	37.0
6	36.33814889525756	37.0	37.0	37.0	35.0	37.0
7	36.2773239879127	37.0	37.0	37.0	35.0	37.0
8	36.180913624728824	37.0	37.0	37.0	35.0	37.0
9	37.80133122759482	39.0	38.0	39.0	35.0	39.0
10	37.63383398309029	39.0	37.0	39.0	35.0	39.0
11	37.38926456164397	39.0	37.0	39.0	35.0	39.0
12	37.394481913165826	39.0	37.0	39.0	35.0	39.0
13	37.387324394059426	39.0	37.0	39.0	35.0	39.0
14	38.64283876955282	40.0	38.0	41.0	35.0	41.0
15	38.75105446427613	40.0	38.0	41.0	35.0	41.0
16	38.74148714125457	40.0	38.0	41.0	35.0	41.0
17	38.63220659516928	40.0	38.0	41.0	34.0	41.0
18	38.41016898038031	40.0	38.0	41.0	34.0	41.0
19	38.161888218150274	40.0	37.0	41.0	34.0	41.0
20	37.75147088138041	40.0	35.0	41.0	34.0	41.0
21	37.74067017944683	39.0	35.0	41.0	34.0	41.0
22	37.73178303548462	39.0	35.0	41.0	34.0	41.0
23	37.68641271136553	39.0	35.0	41.0	34.0	41.0
24	37.62754757868859	39.0	35.0	41.0	34.0	41.0
25	37.58135562440858	39.0	35.0	41.0	34.0	41.0
26	37.52905559681861	39.0	35.0	41.0	34.0	41.0
27	37.475644034334806	39.0	35.0	41.0	34.0	41.0
28	37.39861947459552	39.0	35.0	41.0	34.0	41.0
29	37.276067267234744	39.0	35.0	41.0	33.0	41.0
30	37.19071203123315	39.0	35.0	41.0	33.0	41.0
31	36.94705573967022	39.0	35.0	41.0	33.0	41.0
32	36.60957115107118	39.0	35.0	41.0	32.0	41.0
33	36.125176755522546	38.0	35.0	41.0	30.0	41.0
34	35.91054221521762	38.0	35.0	41.0	30.0	41.0
35	35.72811417671491	38.0	35.0	41.0	29.0	41.0
36	35.711946735957476	38.0	35.0	41.0	29.0	41.0
37	35.60534068941306	38.0	35.0	41.0	29.0	41.0
38	35.49779490337209	38.0	35.0	40.0	28.0	41.0
39	35.40420235817193	38.0	35.0	40.0	27.0	41.0
40	35.34888424024653	38.0	35.0	40.0	27.0	41.0
41	35.2540298391799	38.0	35.0	40.0	27.0	41.0
42	35.18082959437146	38.0	35.0	40.0	26.0	41.0
43	35.07252239992568	37.0	35.0	40.0	25.0	41.0
44	34.963795520528386	37.0	35.0	40.0	25.0	41.0
45	34.88153727003109	37.0	35.0	40.0	24.0	41.0
46	34.787258943747744	37.0	35.0	40.0	24.0	41.0
47	34.64959387661449	37.0	35.0	40.0	23.0	41.0
48	34.60325626971504	36.0	35.0	40.0	23.0	41.0
49	34.53072173207107	36.0	34.0	40.0	24.0	41.0
50	34.46468460839286	36.0	34.0	40.0	24.0	41.0
51	34.064139438107674	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	6.0
14	10.0
15	44.0
16	82.0
17	157.0
18	387.0
19	715.0
20	1356.0
21	2274.0
22	3665.0
23	6111.0
24	10644.0
25	20001.0
26	31821.0
27	39864.0
28	41481.0
29	40215.0
30	41514.0
31	45238.0
32	52135.0
33	66990.0
34	139173.0
35	193545.0
36	220421.0
37	199702.0
38	315318.0
39	669133.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.845186204269396	16.529471547087578	22.629842074280035	32.99550017436299
2	38.30631212702776	18.206157277752546	22.37700406566879	21.11052652955091
3	23.962563542122318	20.022426768710645	36.40689926580809	19.608110423358944
4	22.43484496165648	22.357770450538098	34.68399683859122	20.5233877492142
5	21.05810092326021	29.029360673699383	31.819822107733454	18.09271629530695
6	73.11985576655994	2.102159440133739	21.310938931871455	3.4670458614348747
7	72.52197977389298	1.9829296997361916	19.681216834268326	5.813873692102501
8	62.21444267098894	3.387170338404254	26.07205229675974	8.326334693847064
9	38.55149403641222	13.586541697964085	29.6012339391144	18.26073032650929
10	26.097214720438007	14.818893572284548	38.88313384682106	20.200757860456385
11	23.12590128393718	12.304331811605806	41.82400962054225	22.74575728391477
12	20.964360391264016	13.190431930041926	44.46172253829567	21.38348514039839
13	17.241068623391346	15.793085515360517	45.776564213431506	21.189281647816635
14	14.883877048648442	16.680726190348366	46.95480987431393	21.480586886689267
15	18.081138779403037	16.051198763073142	45.243064811214126	20.624597646309688
16	19.23795669915685	19.14150852231216	40.81564533213699	20.804889446394
17	18.18785733325926	18.908324280618444	42.24383462265468	20.659983763467615
18	19.73994471736156	24.38276201248971	38.360138239274576	17.517155030874154
19	20.71903843128394	20.714930280479326	36.258819102714504	22.307212185522225
20	18.3992403655694	25.57216503748921	37.25439210338722	18.77420249355417
21	18.805760561098705	20.244406962755413	37.168214303554066	23.781618172591816
22	19.48818042998334	21.651588663931324	34.71037303409812	24.14985787198722
23	18.269926982287803	19.52641424258537	38.63757846918163	23.566080305945196
24	22.484096087779978	17.83245560512828	36.156955636172825	23.526492670918913
25	16.843184881258104	19.56427458693244	38.378531550831596	25.21400898097786
26	16.648981388676347	19.684064529712433	37.56717176692033	26.099782314690888
27	20.3317985344172	19.981531994791986	36.663005121650286	23.02366434914053
28	20.878509376153957	20.680664568086296	33.20076766399808	25.240058391761664
29	19.874486656212667	20.715443799329904	34.46995284496446	24.94011669949297
30	22.225982840067353	18.39233120285255	31.94348678365871	27.438199173421385
31	23.557117067826034	19.375019548728968	31.09473349071908	25.97312989272591
32	21.55173258926008	19.71412872423711	33.32952084489723	25.404617841605575
33	17.099057319440934	19.228713359846466	35.44960676126929	28.222622559443305
34	19.38416952097561	20.116073933643094	34.32308645369951	26.176670091681785
35	19.456995830693767	20.155521518073762	35.438916232470916	24.94856641876155
36	21.812553481821197	19.050755736355686	34.53890442153735	24.59778636028576
37	20.72207286085553	21.04316219306161	35.93595579629734	22.298809149785512
38	19.97214860488599	17.834976515849295	37.551532783743674	24.641342095521043
39	21.07612076656227	17.23159186642161	41.14863896497008	20.543648402046045
40	17.98165617298676	17.11969144052775	41.0914049548967	23.807247431588785
41	20.41844316956906	20.666846242652596	34.118846001765576	24.795864586012772
42	19.000804357254133	16.5707397892612	37.07484723981283	27.35360861367183
43	21.405333033313838	18.178193842162045	36.87634886229898	23.540124262225135
44	20.590798769235366	19.069522516167677	36.02400093740532	24.31567777719164
45	20.789390513812958	19.721037886953958	34.72031662638656	24.769254972846525
46	21.900691989992918	18.573930141829237	34.88604316452724	24.639334703650604
47	19.689433135877554	17.943002208597893	36.76248772806656	25.605076927457993
48	20.725527442213956	16.181725918183375	34.125148278568105	28.967598361034565
49	20.26723520984014	15.987709159729105	38.21098435494796	25.534071275482788
50	20.444772681544084	14.796578844050392	38.99293351378075	25.765714960624774
51	18.397046239571484	14.424977930360308	38.56970061384176	28.60827521622645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	445.0
1	3115.0
2	5785.0
3	84407.5
4	163030.0
5	92464.0
6	21898.0
7	19648.0
8	17398.0
9	16964.5
10	16531.0
11	16014.5
12	15498.0
13	14802.5
14	14107.0
15	13097.0
16	12087.0
17	11448.0
18	10809.0
19	10116.5
20	9424.0
21	8808.5
22	8193.0
23	7811.0
24	7429.0
25	7428.0
26	8247.0
27	9067.0
28	9105.0
29	9143.0
30	10152.5
31	11162.0
32	12610.5
33	14059.0
34	16357.5
35	18656.0
36	21543.0
37	24430.0
38	27335.0
39	30240.0
40	34036.0
41	37832.0
42	46019.0
43	54206.0
44	65386.0
45	76566.0
46	107111.0
47	137656.0
48	182210.0
49	226764.0
50	245346.5
51	263929.0
52	227984.5
53	192040.0
54	165926.5
55	139813.0
56	124105.0
57	108397.0
58	101518.5
59	94640.0
60	91410.5
61	88181.0
62	85905.0
63	83629.0
64	79618.5
65	75608.0
66	62973.5
67	50339.0
68	39792.0
69	29245.0
70	23956.5
71	18668.0
72	16597.5
73	14527.0
74	12744.0
75	8515.5
76	6070.0
77	4590.5
78	3111.0
79	2421.0
80	1731.0
81	1238.5
82	746.0
83	514.0
84	282.0
85	231.5
86	181.0
87	130.5
88	80.0
89	56.0
90	32.0
91	27.0
92	22.0
93	14.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2142083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.903661611539395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09605637802237	22.898394530559486
2	10.59216801392005	6.5467355206951305
3	3.8858756517183735	3.6026335861567413
4	2.0033478818475614	2.4764314012324493
5	1.3074516563026908	2.0202521779912535
6	0.9159892665809368	1.6984453400531656
7	0.7145810549166562	1.5458219780612832
8	0.6166164362636515	1.5244564552324187
9	0.5197647894163296	1.4456371652743754
>10	4.933616225348943	29.228355357905045
>50	0.2377773187891765	5.093340151688029
>100	0.16457606567696345	9.110403987236431
>500	0.005937389833174845	1.2302573893894222
>1k	0.005023945243455637	3.0305009454564553
>5k	9.134445897192069E-4	1.9100535199981554
>10k+	3.044815299064023E-4	6.638280493070095
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130400	6.087532555928038	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	10696	0.49932705688808515	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	9357	0.43681780771333323	TruSeq Adapter, Index 14 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7481	0.3492395019240617	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	7412	0.3460183382249894	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	6006	0.28038129241490645	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	5315	0.2481229718923123	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	5027	0.23467811471357555	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	4536	0.21175650056510414	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	4095	0.19116906301016345	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	4040	0.1886014687572797	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	3059	0.1428049239922076	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	3021	0.14103094978112427	No Hit
AGCCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	2653	0.12385141005273839	No Hit
GGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	2569	0.11992999337560684	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2546	0.11885627214258271	TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	2486	0.11605526023034589	No Hit
AGGCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	2384	0.11129353997954326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4516631708481884	0.0
2	0.0	0.0	0.0	1.41315719325535	0.0
3	0.0	0.0	0.0	2.3802532394869855	0.0
4	0.0	0.0	0.0	3.051048908935835	0.0
5	0.0	0.0	0.0	6.152375981696321	0.0
6	0.0	0.0	0.0	7.453399331398456	0.0
7	0.0	0.0	0.0	10.75537222413884	0.0
8	0.0	0.0	0.0	14.52245314490615	0.0
9	0.0	0.0	0.0	17.904161510081543	0.0
10	0.0	0.0	0.0	19.851471675000454	0.0
11	0.0	0.0	0.0	21.47722567239458	0.0
12	0.0	0.0	0.0	22.589974338062532	0.0
13	0.0	0.0	0.0	23.1573193008861	0.0
14	0.0	0.0	0.0	23.562345623395544	0.0
15	0.0	0.0	0.0	24.518424356105715	0.0
16	0.0	0.0	0.0	25.44962076632885	0.0
17	0.0	0.0	0.0	26.651815079060896	0.0
18	0.0	0.0	0.0	27.341797680108566	0.0
19	0.0	0.0	0.0	28.141673315179663	0.0
20	0.0	0.0	0.0	29.0153556141382	0.0
21	0.0	0.0	0.0	29.926104637402005	0.0
22	4.668353187061379E-5	0.0	0.0	30.697363267436415	0.0
23	4.668353187061379E-5	0.0	0.0	31.433235780312902	0.0
24	1.4005059561184136E-4	0.0	0.0	32.01281182848657	0.0
25	1.4005059561184136E-4	0.0	0.0	32.61110797294036	0.0
26	1.4005059561184136E-4	0.0	0.0	33.152963727362575	0.0
27	1.4005059561184136E-4	0.0	0.0	33.70261563160718	0.0
28	1.4005059561184136E-4	0.0	0.0	34.23139999710562	0.0
29	1.4005059561184136E-4	0.0	0.0	34.790481974788094	0.0
30	1.8673412748245515E-4	0.0	0.0	35.330377020871744	0.0
31	1.8673412748245515E-4	0.0	0.0	35.86130882883623	0.0
32	1.8673412748245515E-4	0.0	0.0	36.42281835017597	0.0
33	1.8673412748245515E-4	0.0	0.0	36.95295653809866	0.0
34	1.8673412748245515E-4	0.0	0.0	37.52473643644994	0.0
35	1.8673412748245515E-4	0.0	0.0	38.02588415108098	0.0
36	1.8673412748245515E-4	0.0	0.0	38.59224875973527	0.0
37	1.8673412748245515E-4	0.0	0.0	39.22420373066777	0.0
38	1.8673412748245515E-4	0.0	0.0	39.955127789165964	0.0
39	1.8673412748245515E-4	0.0	0.0	40.64356983366191	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.0346514E-4	45.000004	16
CGTTTTT	31010	0.0	44.245407	1
TACGCGG	245	0.0	44.081635	1
TCATCGG	85	0.0	42.352943	1
TATACGG	75	0.0	42.000004	1
TACGGGT	130	0.0	41.53846	3
ACGGGTA	125	0.0	41.4	4
CATGCGG	270	0.0	40.833332	1
CGGTCTA	265	0.0	40.75472	30
GTTTTTT	34160	0.0	40.409103	2
CACGACG	270	0.0	40.0	25
ATTGCGG	405	0.0	40.0	1
CGTTCAG	475	0.0	39.789474	38
ACCCGCA	1960	0.0	39.719387	31
TCGATGG	255	0.0	39.705883	1
TACTATT	295	0.0	39.66102	45
GCGCGAC	1180	0.0	39.47034	8
GCATAGG	525	0.0	39.428574	1
CAACCCG	120	0.0	39.375004	22
GCGATCG	80	0.0	39.375004	8
>>END_MODULE