FastQCFastQC Report
Thu 26 May 2016
SRR1043260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043260_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2370054
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT907043.827085796357382No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC168150.7094775055758223No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA122260.5158532252851623No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT88010.3713417500191979No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT74020.31231355910034114Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT51620.2178009446198272No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC44970.18974251219592464No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43420.1832025768189248No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC37090.1564943246018867No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG28380.119744107096294No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26790.1130353991934361No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25080.10582037371300401No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT24020.1013479017777654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT222200.045.223671
ATTACGG1950.043.6410261
CACTTAC43000.042.4697736
TACACGG1300.042.461541
AGGGTAC9500.042.3684235
CGGGTAC7450.041.9865765
ACTTACT43300.041.85681537
ACCCGCA45600.041.56140531
CCCGCAC45750.041.37486332
CGACGGT5800.041.24138327
CGCACTT44650.041.20940434
GCACTTA44500.041.14157535
CCGCACT44800.041.1227733
GGGTACC26100.040.9770136
CATATGC20800.040.91346432
CTTACTG44250.040.90621638
GCGCGAC26050.040.8790788
AATGCGG3500.040.742861
CCAGTAG22300.040.63677225
ACGTAGG1700.040.5882381