##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043260_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2370054
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.413230669005856	33.0	31.0	34.0	31.0	34.0
2	32.754718668857336	34.0	31.0	34.0	31.0	34.0
3	32.89095227366128	34.0	31.0	34.0	31.0	34.0
4	36.285285482946804	37.0	37.0	37.0	35.0	37.0
5	36.23907134605372	37.0	37.0	37.0	35.0	37.0
6	36.3549919115767	37.0	37.0	37.0	35.0	37.0
7	36.305165620698936	37.0	37.0	37.0	35.0	37.0
8	36.29454603144063	37.0	37.0	37.0	35.0	37.0
9	37.904969253865104	39.0	38.0	39.0	35.0	39.0
10	37.71078633651385	39.0	37.0	39.0	35.0	39.0
11	37.38483848891207	39.0	37.0	39.0	35.0	39.0
12	37.388273853675905	39.0	37.0	39.0	35.0	39.0
13	37.40090858689296	39.0	37.0	39.0	35.0	39.0
14	38.71453182079396	40.0	38.0	41.0	35.0	41.0
15	38.76935420036843	41.0	38.0	41.0	35.0	41.0
16	38.66527429332834	41.0	38.0	41.0	34.0	41.0
17	38.63266490974467	40.0	38.0	41.0	34.0	41.0
18	38.43833600415856	40.0	37.0	41.0	34.0	41.0
19	38.259235865511926	40.0	37.0	41.0	34.0	41.0
20	37.98401935145782	40.0	35.0	41.0	34.0	41.0
21	37.92553460807222	40.0	35.0	41.0	34.0	41.0
22	37.87923186560306	40.0	35.0	41.0	34.0	41.0
23	37.896538644267174	40.0	35.0	41.0	34.0	41.0
24	37.82099521783048	40.0	35.0	41.0	34.0	41.0
25	37.73996162112762	40.0	35.0	41.0	34.0	41.0
26	37.63495388712662	40.0	35.0	41.0	34.0	41.0
27	37.67752506904906	40.0	35.0	41.0	34.0	41.0
28	37.64883922476028	40.0	35.0	41.0	34.0	41.0
29	37.56122729693079	39.0	35.0	41.0	34.0	41.0
30	37.49042173722624	40.0	35.0	41.0	34.0	41.0
31	37.382025894768645	40.0	35.0	41.0	33.0	41.0
32	37.16914509120889	39.0	35.0	41.0	33.0	41.0
33	36.78685000426151	39.0	35.0	41.0	32.0	41.0
34	36.598382990429755	39.0	35.0	41.0	32.0	41.0
35	36.40107145238041	39.0	35.0	41.0	31.0	41.0
36	36.34717943135473	39.0	35.0	41.0	31.0	41.0
37	36.30216526712049	39.0	35.0	41.0	31.0	41.0
38	36.20569868872186	39.0	35.0	41.0	31.0	41.0
39	36.17829846914881	38.0	35.0	41.0	31.0	41.0
40	36.11090338026053	38.0	35.0	41.0	31.0	41.0
41	36.06763390201236	38.0	35.0	41.0	31.0	41.0
42	35.99895867351545	38.0	35.0	41.0	31.0	41.0
43	35.89271763428175	38.0	35.0	41.0	31.0	41.0
44	35.71855324815384	38.0	35.0	40.0	30.0	41.0
45	35.604251633085156	37.0	35.0	40.0	30.0	41.0
46	35.576259021946335	37.0	35.0	40.0	30.0	41.0
47	35.491305261399106	37.0	35.0	40.0	30.0	41.0
48	35.400282862753336	37.0	35.0	40.0	30.0	41.0
49	35.35353371695329	36.0	35.0	40.0	30.0	41.0
50	35.25928354375048	36.0	35.0	40.0	30.0	41.0
51	35.14452793058724	36.0	35.0	40.0	29.0	41.0
52	34.77598105359625	35.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	3.0
14	12.0
15	33.0
16	71.0
17	162.0
18	371.0
19	728.0
20	1375.0
21	2296.0
22	3784.0
23	5910.0
24	10170.0
25	18260.0
26	27373.0
27	32814.0
28	34056.0
29	34140.0
30	36040.0
31	40955.0
32	50190.0
33	67482.0
34	164946.0
35	233260.0
36	217471.0
37	237128.0
38	344793.0
39	804215.0
40	2009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.72269830138891	17.848538472119202	23.904560824352526	28.52420240213936
2	34.289725044239496	20.29190052209781	24.518513080292685	20.899861353370007
3	27.222713068984927	20.91129569199689	32.31095156481666	19.555039674201517
4	24.2253973960087	23.81675691777487	29.946153125624985	22.01169256059145
5	22.598936564314567	31.802777489458045	26.91174969009145	18.686536256135934
6	79.89885462525326	2.146955301440389	15.302900271470607	2.651289801835739
7	79.8941711876607	2.0894882563857196	13.88588614436633	4.130454411587246
8	70.91716053727046	3.604432641619136	18.86627899617477	6.612127824935635
9	43.467110875954724	15.6939884070152	23.36541699049895	17.473483726531125
10	28.939509395144587	16.66295367109779	32.68870667081847	21.70883026293916
11	24.333369619426392	14.219929166170896	37.54091678923771	23.905784425164995
12	21.377740760337105	15.50850740109719	40.59434932706175	22.51940251150396
13	18.081950875380898	19.12083015830019	41.485215104803515	21.31200386151539
14	16.97379047059687	18.196673999832917	41.81731724256072	23.012218287009496
15	20.600163540577558	15.074213498932936	41.50348473072765	22.822138229761855
16	21.178968918007776	18.359286328497156	36.191917990054236	24.269826763440832
17	20.66940246931083	19.86701568825014	36.605832609721126	22.857749232717907
18	22.3813043922206	23.804478716518695	33.595352679727974	20.218864211532733
19	23.875658529299333	20.43869042646286	31.74712474905635	23.938526295181457
20	22.63252229696032	24.000043880856722	34.03732573181877	19.330108090364188
21	21.433477887001732	18.792019084797225	34.03935100212907	25.73515202607198
22	21.596427760717688	21.06302219274329	32.060155591391585	25.280394455147437
23	21.77368110600012	19.377026852552724	35.79800291470152	23.051289126745637
24	25.245922666740928	17.998661633869943	32.654994358778325	24.100421340610804
25	19.810392505824762	19.787734794228317	33.7961497923676	26.60572290757932
26	18.667211801925188	19.1291422051987	35.35873022302445	26.844915769851657
27	22.29919655839065	19.827733882856677	35.547797645116944	22.32527191363572
28	22.911798634123947	21.089814831223254	31.75273643554113	24.245650099111664
29	22.860407400000167	18.79653374986393	35.42185114769537	22.921207702440537
30	25.975990420471433	19.38048668933282	32.41719386984432	22.226329020351436
31	27.642830078977106	18.922100509102325	30.71638874050971	22.718680671410862
32	26.98896312067151	18.57966105413632	30.967648838380896	23.46372698681127
33	22.091564158453775	20.25810382379473	36.25520768725101	21.395124330500487
34	24.64808818702021	21.53727299040444	32.426898290081155	21.387740532494195
35	26.384462126179404	21.73718404728331	31.117054716896746	20.76129910964054
36	24.519821067368085	24.42138449166137	30.05353464520218	21.005259795768367
37	23.64595912160651	25.87856647991987	29.70050471423858	20.774969684235042
38	22.074686905867967	23.35090255327516	27.927760295756975	26.646650245099902
39	23.372083505270343	21.1386322843277	32.78490701055757	22.70437719984439
40	19.366098831503418	20.865811496278145	35.037134175001924	24.73095549721652
41	21.472548726737873	24.220587379021744	29.26237123711105	25.04449265712933
42	21.247828108557865	18.642233468098198	32.101125122043634	28.008813301300307
43	23.67912292293762	19.249814561187215	33.32734190866537	23.74372060720979
44	23.21655962269214	20.424471341159315	32.8065098938674	23.552459142281144
45	22.921967178806895	20.607294179795062	32.126947318499916	24.343791322898127
46	23.64005208320148	20.99428958158759	31.09916482915579	24.266493506055138
47	20.73699586591698	22.15320832352343	33.573074706314706	23.536721104244883
48	22.388392838306636	20.496410630306315	31.088827511946988	26.026369019440065
49	22.131394474556277	18.61611591972166	34.62866246929395	24.623827136428115
50	21.15664875146305	18.81691303236129	35.374130716009	24.65230750016666
51	20.301351783545858	17.0078825208202	34.50406615207924	28.186699543554706
52	18.35072112280986	18.85767159735601	37.48311219913133	25.3084950807028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	664.0
1	4331.0
2	7998.0
3	62975.0
4	117952.0
5	67205.5
6	16459.0
7	15173.5
8	13888.0
9	13661.5
10	13435.0
11	13094.5
12	12754.0
13	12231.5
14	10936.5
15	10164.0
16	9635.0
17	9106.0
18	8542.5
19	7979.0
20	7653.5
21	7328.0
22	7139.0
23	6950.0
24	7134.0
25	7318.0
26	8209.0
27	9100.0
28	9159.0
29	9218.0
30	10130.0
31	11042.0
32	13769.0
33	16496.0
34	18552.0
35	20608.0
36	23783.0
37	26958.0
38	29213.5
39	36741.0
40	42013.0
41	54874.0
42	67735.0
43	92849.5
44	117964.0
45	150048.5
46	182133.0
47	201958.0
48	221783.0
49	212914.0
50	204045.0
51	185565.0
52	167085.0
53	149762.5
54	132440.0
55	126410.5
56	120381.0
57	119294.0
58	118207.0
59	117018.5
60	115830.0
61	114316.5
62	112803.0
63	115527.0
64	104182.0
65	90113.0
66	75122.5
67	60132.0
68	50119.0
69	40106.0
70	34762.5
71	29419.0
72	28137.5
73	26856.0
74	21499.0
75	16142.0
76	12300.0
77	8458.0
78	6418.0
79	4378.0
80	3503.0
81	2628.0
82	2082.0
83	1536.0
84	1056.0
85	576.0
86	431.0
87	286.0
88	183.5
89	64.5
90	48.0
91	35.0
92	22.0
93	15.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2370054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.621928544078845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.83664710551342	19.390520146332655
2	11.13675514833387	5.929637995436929
3	4.268855564404791	3.409355034017331
4	2.109652453039696	2.2465206743065376
5	1.2391674533167085	1.6494513698172786
6	0.8156219292577785	1.3028057231810588
7	0.5716609864832584	1.0653102553516447
8	0.44062950294385383	0.9384325713427405
9	0.3503974673773615	0.8395430704691684
>10	5.367005707558362	34.61524801699176
>50	0.7028598999209028	12.173918390081786
>100	0.14797686297508328	6.715852098738089
>500	0.0067042074093442835	1.225725303361467
>1k	0.005107967549976596	2.5012122323725063
>5k	4.7887195781030597E-4	0.9079053219497205
>10k+	4.7887195781030597E-4	5.08856179624944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90704	3.827085796357382	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	16815	0.7094775055758223	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	12226	0.5158532252851623	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	8801	0.3713417500191979	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT	7402	0.31231355910034114	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	5162	0.2178009446198272	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4497	0.18974251219592464	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4342	0.1832025768189248	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	3709	0.1564943246018867	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	2838	0.119744107096294	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2679	0.1130353991934361	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2508	0.10582037371300401	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	2402	0.1013479017777654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.219313146451516E-5	0.0	0.0	0.3204568334729926	0.0
2	4.219313146451516E-5	0.0	0.0	1.0856714657134394	0.0
3	4.219313146451516E-5	0.0	0.0	1.7366270979479792	0.0
4	4.219313146451516E-5	0.0	0.0	2.1545078719725375	0.0
5	4.219313146451516E-5	0.0	0.0	4.457282407911381	0.0
6	4.219313146451516E-5	0.0	0.0	5.0716565951661865	0.0
7	4.219313146451516E-5	0.0	0.0	7.030219564617515	0.0
8	4.219313146451516E-5	0.0	0.0	9.265147545161419	0.0
9	4.219313146451516E-5	0.0	0.0	11.167045139055903	0.0
10	4.219313146451516E-5	0.0	0.0	12.387650239192862	0.0
11	4.219313146451516E-5	0.0	0.0	13.491971069013617	0.0
12	4.219313146451516E-5	0.0	0.0	14.318365741877612	0.0
13	4.219313146451516E-5	0.0	0.0	14.70831466287266	0.0
14	4.219313146451516E-5	0.0	0.0	14.984890639622558	0.0
15	4.219313146451516E-5	0.0	0.0	15.77689791034297	0.0
16	4.219313146451516E-5	0.0	0.0	16.627975565113708	0.0
17	4.219313146451516E-5	0.0	0.0	17.69554617742887	0.0
18	8.438626292903032E-5	0.0	0.0	18.31439283661891	0.0
19	8.438626292903032E-5	0.0	0.0	18.975770172325188	0.0
20	1.2657939439354547E-4	0.0	0.0	19.73980339688463	0.0
21	1.2657939439354547E-4	0.0	0.0	20.4594494471434	0.0
22	2.1096565732257576E-4	0.0	0.0	21.17880014548192	0.0
23	2.1096565732257576E-4	0.0	0.0	21.836295713093456	0.0
24	5.907038405032122E-4	0.0	0.0	22.354891491923812	0.0
25	5.907038405032122E-4	0.0	0.0	22.864415747489296	0.0
26	5.907038405032122E-4	0.0	0.0	23.336303729788437	0.0
27	5.907038405032122E-4	0.0	0.0	23.859498559948424	0.0
28	5.907038405032122E-4	0.0	0.0	24.372356072899606	0.0
29	5.907038405032122E-4	0.0	0.0	24.90171110025341	0.0
30	5.907038405032122E-4	0.0	0.0	25.41566563462267	0.0
31	5.907038405032122E-4	0.0	0.0	25.894895221796634	0.0
32	6.328969719677273E-4	0.0	0.0	26.4518867502597	0.0
33	6.328969719677273E-4	0.0	0.0	26.969427700803443	0.0
34	6.328969719677273E-4	0.0	0.0	27.537136284658494	0.0
35	6.328969719677273E-4	0.0	0.0	28.021597820134055	0.0
36	6.750901034322425E-4	0.0	0.0	28.54842125959999	0.0
37	6.750901034322425E-4	0.0	0.0	29.118661431342915	0.0
38	6.750901034322425E-4	0.0	0.0	29.754680695039017	0.0
39	6.750901034322425E-4	0.0	0.0	30.46614127779367	0.0
40	6.750901034322425E-4	0.0	0.0	31.138446634549254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22220	0.0	45.22367	1
ATTACGG	195	0.0	43.641026	1
CACTTAC	4300	0.0	42.46977	36
TACACGG	130	0.0	42.46154	1
AGGGTAC	950	0.0	42.368423	5
CGGGTAC	745	0.0	41.986576	5
ACTTACT	4330	0.0	41.856815	37
ACCCGCA	4560	0.0	41.561405	31
CCCGCAC	4575	0.0	41.374863	32
CGACGGT	580	0.0	41.241383	27
CGCACTT	4465	0.0	41.209404	34
GCACTTA	4450	0.0	41.141575	35
CCGCACT	4480	0.0	41.12277	33
GGGTACC	2610	0.0	40.977013	6
CATATGC	2080	0.0	40.913464	32
CTTACTG	4425	0.0	40.906216	38
GCGCGAC	2605	0.0	40.879078	8
AATGCGG	350	0.0	40.74286	1
CCAGTAG	2230	0.0	40.636772	25
ACGTAGG	170	0.0	40.588238	1
>>END_MODULE