FastQCFastQC Report
Thu 26 May 2016
SRR1043259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043259_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2341937
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870283.7160692196246097No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC162200.6925890833100976No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG100200.4278509626860159No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC86920.37114576523621257No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT73420.3135011744551625No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC54120.23109075948669838No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC43590.18612797867747938No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC41690.1780150362712575No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG37220.1589282717681987No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC26460.11298339793085808No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC26170.11174510672148738No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC25100.10717623915587823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATA600.045.00000443
CGTTTTT211500.044.2446821
CGGGTAC6150.042.4390265
TACGGGA3450.041.739133
ATTACGG1300.041.538461
CACTTAC43050.041.13240436
CACGACG5700.041.0526325
GCGCGAC22700.040.8370068
AGGGTAC10800.040.8333365
GCACTTA43500.040.7586235
TAGGGCG5300.040.754724
CGACGGT5800.040.73275827
CGGTCTA5800.040.73275830
CGCACTT43450.040.70195434
CATATGC19550.040.626632
ACCCGCA44050.040.60726531
ACTTACT43500.040.55172337
TACGCGG2450.040.4081651
ACTACGG1450.040.3448261
CCCGCAC44750.040.12290632