##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043259_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2341937
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42127392837638	33.0	31.0	34.0	31.0	34.0
2	32.70514450217918	34.0	31.0	34.0	31.0	34.0
3	32.81580076663036	34.0	31.0	34.0	31.0	34.0
4	36.265399538928676	37.0	37.0	37.0	35.0	37.0
5	36.260814018481284	37.0	37.0	37.0	35.0	37.0
6	36.382085000578584	37.0	37.0	37.0	35.0	37.0
7	36.34017354010804	37.0	37.0	37.0	35.0	37.0
8	36.25394876121774	37.0	37.0	37.0	35.0	37.0
9	37.87616703609021	39.0	38.0	39.0	35.0	39.0
10	37.66446748994529	39.0	37.0	39.0	35.0	39.0
11	37.381233568622896	39.0	37.0	39.0	35.0	39.0
12	37.39263353369454	39.0	37.0	39.0	35.0	39.0
13	37.41972307538589	39.0	37.0	39.0	35.0	39.0
14	38.73123060099396	40.0	38.0	41.0	35.0	41.0
15	38.79583011840199	41.0	38.0	41.0	35.0	41.0
16	38.726251816338355	40.0	38.0	41.0	35.0	41.0
17	38.66469465233266	40.0	38.0	41.0	34.0	41.0
18	38.49223356563392	40.0	38.0	41.0	34.0	41.0
19	38.333545693159124	40.0	37.0	41.0	35.0	41.0
20	38.074406783786245	40.0	35.0	41.0	34.0	41.0
21	38.02082037219618	40.0	35.0	41.0	34.0	41.0
22	37.98757268022154	40.0	35.0	41.0	34.0	41.0
23	37.97367990684634	40.0	35.0	41.0	34.0	41.0
24	37.89991746148594	40.0	35.0	41.0	34.0	41.0
25	37.88133839637872	40.0	35.0	41.0	34.0	41.0
26	37.84446379215154	40.0	35.0	41.0	34.0	41.0
27	37.834191526074356	40.0	35.0	41.0	34.0	41.0
28	37.74387398123861	40.0	35.0	41.0	34.0	41.0
29	37.68816966468355	40.0	35.0	41.0	34.0	41.0
30	37.58151564282045	40.0	35.0	41.0	34.0	41.0
31	37.46407738551464	40.0	35.0	41.0	34.0	41.0
32	37.266424758650636	40.0	35.0	41.0	33.0	41.0
33	36.88944536082738	39.0	35.0	41.0	33.0	41.0
34	36.66284746344586	39.0	35.0	41.0	32.0	41.0
35	36.465009946894384	39.0	35.0	41.0	31.0	41.0
36	36.39687190560634	39.0	35.0	41.0	32.0	41.0
37	36.29758187346628	39.0	35.0	41.0	31.0	41.0
38	36.201889717784894	38.0	35.0	41.0	31.0	41.0
39	36.12995524644771	38.0	35.0	41.0	31.0	41.0
40	36.04579670588919	38.0	35.0	41.0	31.0	41.0
41	35.96512246059565	38.0	35.0	41.0	31.0	41.0
42	35.88355109467078	38.0	35.0	41.0	31.0	41.0
43	35.76413370641482	38.0	35.0	40.0	30.0	41.0
44	35.64443962412311	37.0	35.0	40.0	30.0	41.0
45	35.56239642654777	37.0	35.0	40.0	30.0	41.0
46	35.481334468006615	37.0	35.0	40.0	30.0	41.0
47	35.33169380730566	37.0	35.0	40.0	30.0	41.0
48	35.26495119211149	36.0	35.0	40.0	29.0	41.0
49	35.206996174534154	36.0	35.0	40.0	29.0	41.0
50	35.13644431938177	36.0	35.0	40.0	29.0	41.0
51	34.75318806611792	35.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	5.0
14	17.0
15	37.0
16	69.0
17	175.0
18	330.0
19	631.0
20	1274.0
21	2137.0
22	3369.0
23	5251.0
24	8597.0
25	15326.0
26	24922.0
27	32184.0
28	34098.0
29	34215.0
30	35254.0
31	40128.0
32	48367.0
33	65583.0
34	160279.0
35	233518.0
36	209330.0
37	234505.0
38	361744.0
39	790435.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.023950259977106	17.948219785587742	23.835611290995445	28.19221866343971
2	34.046731402253776	20.40516034376672	24.55104471213359	20.997063541845918
3	27.38293130857064	20.928060831696154	32.056285032432555	19.63272282730065
4	24.291857552103238	23.95068697407317	29.599130975769206	22.158324498054387
5	22.77904999152411	31.948212099642305	26.61472106209518	18.658016846738406
6	80.12585308656894	2.105735551383321	15.07867205650707	2.68973930554067
7	80.09737238875341	2.0734972802428078	13.65805314148075	4.171077189523031
8	71.05195400217855	3.569523859950118	18.667368080353995	6.711154057517346
9	43.522391934539655	15.721046296292343	23.18175083275084	17.574810936417162
10	29.179649153670656	16.638790881223535	32.42849829009064	21.75306167501517
11	24.49843868558377	14.176427461541449	37.37017690911412	23.954956943760656
12	21.509929601009762	15.460748944143246	40.48789527643144	22.54142617841556
13	18.17721825992757	19.119984867227426	41.372462196890865	21.330334675954134
14	17.066001348456428	18.199678300483747	41.70282975161159	23.031490599448233
15	20.631127139628436	15.0913538664789	41.470244502734275	22.80727449115839
16	21.21299590894204	18.288920666952187	36.148495881827735	24.349587542278037
17	20.801413530765345	19.784135952418875	36.46263755173602	22.95181296507976
18	22.417212760206613	23.934034092291977	33.530363967946194	20.118389179555216
19	24.01183293999796	20.532661638635027	31.454347405587768	24.001158015779247
20	22.57643992985294	24.214400301972255	33.8086378924796	19.400521875695205
21	21.46774230049741	18.86775775778768	33.88327696261684	25.78122297909807
22	21.616081047440645	21.198648810792093	31.76810477822418	25.41716536354308
23	21.8519968726742	19.40726842780143	35.543782774686086	23.196951924838284
24	25.333046960699622	17.926442940181566	32.506681435068494	24.233828664050314
25	19.6474115230256	19.837553273209313	33.757782553501656	26.757252650263435
26	18.726122863253796	19.032236990149606	35.253894532602715	26.987745613993887
27	22.33001997918817	19.791437600584473	35.429048689183354	22.449493731044004
28	22.964067778082846	21.14655518060477	31.531292259356253	24.358084781956133
29	22.876704198276894	18.841753642390895	35.24582428989337	23.035717869438844
30	26.09468145385636	19.337240924926675	32.25133724775688	22.31674037346009
31	27.792549500691095	18.948203986699898	30.478915530178654	22.780330982430357
32	27.074425998649836	18.501437058298322	30.86662877780231	23.557508165249537
33	22.184072415269924	20.11352141411148	36.229625305889954	21.47278086472864
34	24.677222316398776	21.532645839747182	32.33323526636284	21.456896577491197
35	26.49968807871433	21.661513524915488	31.07427740370471	20.76452099266547
36	24.551386309708587	24.43259575300275	29.949353889536738	21.066664047751928
37	23.65473537503357	25.853513565907193	29.707033109771952	20.78471794928728
38	22.23578174818537	23.285425696762978	27.798356659466073	26.680435895585575
39	23.465917315452977	21.169826515401567	32.67278325591166	22.69147291323379
40	19.372638973635926	20.841978242796454	35.00410130588483	24.78128147768279
41	21.548530126984627	24.19164136353796	29.139169841033297	25.12065866844411
42	21.39408532338829	18.57560643176994	32.06533736817002	27.964970876671746
43	23.86225590184535	19.100001409089998	33.28223602940643	23.755506659658224
44	23.243494594431873	20.27782984768591	32.85365917187354	23.625016386008678
45	23.059757798779387	20.444999160950957	32.17772297034463	24.317520069925024
46	23.85832752973287	20.948300488014834	31.03589037621422	24.15748160603808
47	20.92157047777118	21.917242009498974	33.574985151180414	23.586202361549436
48	22.49240692640323	20.384109393207417	31.0051892941612	26.118294386228154
49	22.28138502444771	18.505151931926438	34.62889906944551	24.584563974180348
50	21.2830234118168	18.696062276653898	35.32503222759622	24.695882083933085
51	20.322237532435757	17.01373691948161	34.31099982621223	28.353025721870402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	640.0
1	4173.0
2	7706.0
3	60380.0
4	113054.0
5	65116.5
6	17179.0
7	15500.0
8	13821.0
9	13426.0
10	13031.0
11	12703.5
12	12376.0
13	11809.0
14	11242.0
15	10450.0
16	9658.0
17	9078.5
18	8499.0
19	7881.5
20	7264.0
21	7136.5
22	7009.0
23	6857.0
24	6705.0
25	6821.5
26	7978.0
27	9018.0
28	8996.5
29	8975.0
30	10991.0
31	13007.0
32	14819.0
33	16631.0
34	19870.0
35	23109.0
36	25870.5
37	28632.0
38	31679.5
39	34727.0
40	43106.5
41	51486.0
42	65886.5
43	80287.0
44	106894.0
45	133501.0
46	169790.5
47	206080.0
48	227904.0
49	249728.0
50	221055.0
51	192382.0
52	166274.5
53	140167.0
54	131608.5
55	123050.0
56	121746.5
57	120443.0
58	119704.0
59	118965.0
60	116520.0
61	114075.0
62	111970.0
63	109865.0
64	109621.0
65	109377.0
66	91377.5
67	73378.0
68	60578.5
69	47779.0
70	40599.0
71	33419.0
72	30450.0
73	27481.0
74	23120.0
75	14890.5
76	11022.0
77	8290.0
78	5558.0
79	4304.5
80	3051.0
81	2348.5
82	1646.0
83	1205.5
84	765.0
85	509.5
86	254.0
87	185.0
88	116.0
89	84.0
90	52.0
91	34.0
92	16.0
93	12.5
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2341937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.329744862418902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29178774460159	19.034243269635017
2	11.35534828140428	5.979668461465634
3	4.251770168403905	3.35844071243156
4	2.16626955625476	2.2814929887765296
5	1.2698001712095774	1.6716757267102005
6	0.789001394022331	1.2464523240420493
7	0.5893343365848274	1.0861915904658987
8	0.4701150528708327	0.9902407518457279
9	0.3852989744388833	0.9130341323454715
>10	5.580272673222092	35.34743669238337
>50	0.6886583780160943	11.791368324472739
>100	0.1497423710564671	6.810935097724517
>500	0.006382272969682022	1.1818636983953308
>1k	0.005236736795636531	2.5023849264649547
>5k	4.909440745909248E-4	0.9240675517075456
>10k+	4.909440745909248E-4	4.880503751133557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87028	3.7160692196246097	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16220	0.6925890833100976	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10020	0.4278509626860159	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	8692	0.37114576523621257	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	7342	0.3135011744551625	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	5412	0.23109075948669838	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4359	0.18612797867747938	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4169	0.1780150362712575	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	3722	0.1589282717681987	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2646	0.11298339793085808	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC	2617	0.11174510672148738	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2510	0.10717623915587823	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32157141716450954	0.0
2	0.0	0.0	0.0	1.0957169215055742	0.0
3	0.0	0.0	0.0	1.7474850946033134	0.0
4	0.0	0.0	0.0	2.190964146345525	0.0
5	0.0	0.0	0.0	4.554904764731075	0.0
6	0.0	0.0	0.0	5.187842371507005	0.0
7	0.0	0.0	0.0	7.197290106437534	0.0
8	0.0	0.0	0.0	9.516054445529491	0.0
9	0.0	0.0	0.0	11.498003575672616	0.0
10	0.0	0.0	0.0	12.782068860093162	0.0
11	0.0	0.0	0.0	13.92603643906732	0.0
12	0.0	0.0	0.0	14.790150204723696	0.0
13	4.2699696874851884E-5	0.0	0.0	15.19720641503166	0.0
14	4.2699696874851884E-5	0.0	0.0	15.476846729865064	0.0
15	4.2699696874851884E-5	0.0	0.0	16.295485318349726	0.0
16	4.2699696874851884E-5	0.0	0.0	17.170530206406063	0.0
17	4.2699696874851884E-5	0.0	0.0	18.2771782503116	0.0
18	4.2699696874851884E-5	0.0	0.0	18.908749466787537	4.2699696874851884E-5
19	4.2699696874851884E-5	0.0	0.0	19.600740754341384	4.2699696874851884E-5
20	8.539939374970377E-5	0.0	0.0	20.40242756316673	4.2699696874851884E-5
21	8.539939374970377E-5	0.0	0.0	21.140064826679794	4.2699696874851884E-5
22	1.2809909062455567E-4	0.0	0.0	21.88350924896784	4.2699696874851884E-5
23	1.7079878749940754E-4	0.0	0.0	22.550563913546778	4.2699696874851884E-5
24	2.988978781239632E-4	0.0	0.0	23.08063795055119	4.2699696874851884E-5
25	2.988978781239632E-4	0.0	0.0	23.61212107755247	4.2699696874851884E-5
26	2.988978781239632E-4	0.0	0.0	24.08399542771646	4.2699696874851884E-5
27	2.988978781239632E-4	0.0	0.0	24.62252400470209	4.2699696874851884E-5
28	3.4159757499881507E-4	0.0	0.0	25.139275736281547	4.2699696874851884E-5
29	3.84297271873667E-4	0.0	0.0	25.671740956310952	4.2699696874851884E-5
30	3.84297271873667E-4	0.0	0.0	26.190627672734152	4.2699696874851884E-5
31	3.84297271873667E-4	0.0	0.0	26.67586702801997	4.2699696874851884E-5
32	3.84297271873667E-4	0.0	0.0	27.237410741621144	4.2699696874851884E-5
33	3.84297271873667E-4	0.0	0.0	27.746988924125628	4.2699696874851884E-5
34	3.84297271873667E-4	0.0	0.0	28.320915549820512	4.2699696874851884E-5
35	3.84297271873667E-4	0.0	0.0	28.81038217509694	4.2699696874851884E-5
36	3.84297271873667E-4	0.0	0.0	29.330806080607633	4.2699696874851884E-5
37	4.2699696874851887E-4	0.0	0.0	29.921300188689962	4.2699696874851884E-5
38	4.2699696874851887E-4	0.0	0.0	30.56683420604397	4.2699696874851884E-5
39	4.2699696874851887E-4	0.0	0.0	31.284274512935234	4.2699696874851884E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGATA	60	0.0	45.000004	43
CGTTTTT	21150	0.0	44.244682	1
CGGGTAC	615	0.0	42.439026	5
TACGGGA	345	0.0	41.73913	3
ATTACGG	130	0.0	41.53846	1
CACTTAC	4305	0.0	41.132404	36
CACGACG	570	0.0	41.05263	25
GCGCGAC	2270	0.0	40.837006	8
AGGGTAC	1080	0.0	40.833336	5
GCACTTA	4350	0.0	40.75862	35
TAGGGCG	530	0.0	40.75472	4
CGACGGT	580	0.0	40.732758	27
CGGTCTA	580	0.0	40.732758	30
CGCACTT	4345	0.0	40.701954	34
CATATGC	1955	0.0	40.6266	32
ACCCGCA	4405	0.0	40.607265	31
ACTTACT	4350	0.0	40.551723	37
TACGCGG	245	0.0	40.408165	1
ACTACGG	145	0.0	40.344826	1
CCCGCAC	4475	0.0	40.122906	32
>>END_MODULE