FastQCFastQC Report
Thu 26 May 2016
SRR1043258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043258_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2192245
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT788633.597362521068585No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC187330.8545121553476002No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA128060.5841500379747702No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT52700.24039283930400115RNA PCR Primer, Index 34 (96% over 26bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC47720.21767640021986595No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43030.1962828059819956No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT38860.17726120939949688RNA PCR Primer, Index 34 (96% over 27bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34000.15509215438967816No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT33800.15417984759915065TruSeq Adapter, Index 18 (95% over 24bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27820.12690187456237784No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC24690.11262427329062218RNA PCR Primer, Index 34 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187600.045.0314521
CATGCGG3950.044.8354451
ACGGGTA1600.044.56254
ACGTAGG1350.044.29631
ATAACGG1300.044.230771
ACCGGTA2150.043.86046641
CACGACG3800.043.5789525
CCGGTAT2200.042.86363642
GCACTTA46850.042.7598735
CACTTAC47000.042.62340536
ACTTACT47200.042.3453437
ACCCGCA47950.042.3065731
ACGGGAT3100.042.290324
CGCACTT47700.042.19077734
CCGCACT47800.042.15062733
ATTACGG1750.042.0571441
CCCGCAC48600.041.93004232
CTTACTG48300.041.19047538
TTATGAC52000.041.17884426
CCCAACT18300.041.09836213