##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043258_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2192245
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3642877506848	33.0	31.0	34.0	31.0	34.0
2	32.71790881037475	34.0	31.0	34.0	31.0	34.0
3	32.88188044675663	34.0	31.0	34.0	31.0	34.0
4	36.273233602996015	37.0	37.0	37.0	35.0	37.0
5	36.23111194232396	37.0	37.0	37.0	35.0	37.0
6	36.325615521987736	37.0	37.0	37.0	35.0	37.0
7	36.278320625659994	37.0	37.0	37.0	35.0	37.0
8	36.27014681297027	37.0	37.0	37.0	35.0	37.0
9	37.871673102230815	39.0	38.0	39.0	35.0	39.0
10	37.64671831843612	39.0	37.0	39.0	35.0	39.0
11	37.313039829033706	39.0	37.0	39.0	35.0	39.0
12	37.31461629516774	39.0	37.0	39.0	35.0	39.0
13	37.31844752753456	39.0	37.0	39.0	35.0	39.0
14	38.62235425328829	40.0	38.0	41.0	35.0	41.0
15	38.679076015682554	40.0	38.0	41.0	35.0	41.0
16	38.55929013408629	40.0	37.0	41.0	34.0	41.0
17	38.50296568129931	40.0	37.0	41.0	34.0	41.0
18	38.34760029102586	40.0	37.0	41.0	34.0	41.0
19	38.1616489032932	40.0	37.0	41.0	34.0	41.0
20	37.896459109269266	40.0	35.0	41.0	34.0	41.0
21	37.84730721246941	40.0	35.0	41.0	34.0	41.0
22	37.80858936843282	40.0	35.0	41.0	34.0	41.0
23	37.80760407709905	40.0	35.0	41.0	34.0	41.0
24	37.71780343894045	40.0	35.0	41.0	34.0	41.0
25	37.61132537649761	40.0	35.0	41.0	34.0	41.0
26	37.4866121259257	39.0	35.0	41.0	34.0	41.0
27	37.523121731375824	39.0	35.0	41.0	34.0	41.0
28	37.463412620395985	39.0	35.0	41.0	34.0	41.0
29	37.40425636733121	39.0	35.0	41.0	33.0	41.0
30	37.313715392212096	39.0	35.0	41.0	33.0	41.0
31	37.22376832881361	39.0	35.0	41.0	33.0	41.0
32	36.99467121603653	39.0	35.0	41.0	33.0	41.0
33	36.64161989193726	39.0	35.0	41.0	31.0	41.0
34	36.44202495615225	39.0	35.0	41.0	31.0	41.0
35	36.31108338712142	39.0	35.0	41.0	31.0	41.0
36	36.28448599495038	39.0	35.0	41.0	31.0	41.0
37	36.21744969198242	38.0	35.0	41.0	31.0	41.0
38	36.12541527064721	38.0	35.0	41.0	31.0	41.0
39	36.074916809024536	38.0	35.0	41.0	31.0	41.0
40	35.983402858713326	38.0	35.0	41.0	31.0	41.0
41	35.93312471918057	38.0	35.0	41.0	31.0	41.0
42	35.82676890584766	37.0	35.0	41.0	31.0	41.0
43	35.70881767320714	37.0	35.0	40.0	31.0	41.0
44	35.51780708816761	37.0	35.0	40.0	30.0	41.0
45	35.38280940314609	37.0	35.0	40.0	30.0	41.0
46	35.31915730221759	36.0	35.0	40.0	29.0	41.0
47	35.22759910502704	36.0	35.0	40.0	29.0	41.0
48	35.104265262322414	36.0	35.0	40.0	29.0	41.0
49	35.045600286464335	35.0	35.0	40.0	29.0	41.0
50	34.94021927293711	35.0	35.0	40.0	29.0	41.0
51	34.80395256916996	35.0	35.0	40.0	29.0	41.0
52	34.410382963583	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	12.0
14	19.0
15	31.0
16	90.0
17	197.0
18	433.0
19	800.0
20	1466.0
21	2314.0
22	3832.0
23	5858.0
24	9875.0
25	16426.0
26	24616.0
27	30755.0
28	32953.0
29	33579.0
30	35694.0
31	41206.0
32	49590.0
33	65606.0
34	158942.0
35	227345.0
36	201039.0
37	231715.0
38	335947.0
39	680253.0
40	1643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.681744056891453	17.92933727753969	23.674634906226267	27.71428375934259
2	32.94900889271044	20.739972037796868	24.95314164247153	21.357877427021158
3	27.384712931264527	21.29903363903213	31.545926664218644	19.770326765484697
4	23.973232918765923	23.977976914076667	29.58309860439869	22.465691562758725
5	22.77021956943681	32.67549931691029	26.225992076615523	18.328289037037372
6	82.00401871141227	2.1034601515797733	13.515095256232765	2.377425880775187
7	82.41250407687097	2.0062082477095395	12.304874683258486	3.276412992161004
8	74.34844189404014	3.497373696826769	16.405420014642523	5.748764394490579
9	45.78730935639037	15.820859438612016	21.535777251174025	16.85605395382359
10	29.188799609532694	15.922079877021044	31.09989987432974	23.78922063911652
11	25.445331155961128	13.514958410214186	38.059204149171286	22.980506284653405
12	22.056339505849028	15.599989964625305	39.282881247305845	23.060789282219826
13	18.703383973962765	19.059457314305657	40.996695168651314	21.240463543080267
14	17.588773152635767	17.715401335160987	41.49194090988918	23.203884602314066
15	21.961550830313218	14.549012541937604	40.252891442334224	23.23654518541495
16	22.151401873421996	17.6005875255731	35.40329662058757	24.844713980417335
17	22.547844789245726	19.403716281711215	35.65249322042016	22.395945708622897
18	23.6086295099316	23.66195384183793	31.792158266982018	20.937258381248448
19	25.36523061975281	20.185791277890928	30.653006392989834	23.795971709366427
20	24.697604510444773	23.027855007081783	32.7057879023558	19.568752580117643
21	22.370401118488125	18.53679675401244	33.28752032733568	25.80528180016376
22	23.477531024132794	20.349459116111564	31.43736215614587	24.73564770360977
23	23.777451881518715	18.9566403390132	34.45344840562984	22.812459373838234
24	26.549496064536584	17.267732393049133	30.817905845377684	25.3648656970366
25	21.338582138401502	19.241827441732106	32.53619006999674	26.883400349869653
26	20.07740923117626	19.08924413101638	33.8236830281287	27.009663609678665
27	23.216109513307135	19.81612456636918	33.25134736309126	23.716418557232426
28	24.469983966208154	20.848855853246327	30.73903692333658	23.942123257208934
29	25.46590367408752	18.207727694669163	33.48270836516904	22.843660266074277
30	27.959967978031653	19.389940449174247	30.377945895645787	22.272145677148313
31	30.180112168119894	19.095721509229126	27.882330670157764	22.84183565249322
32	30.08755864422088	18.93013782674838	27.720396214839127	23.261907314191614
33	24.77451197288624	20.174524289027914	31.341798019838112	23.709165718247732
34	25.771070295518978	22.122664209520376	29.871980549619227	22.23428494534142
35	27.631172610725535	23.742282454743883	27.55873545155765	21.067809482972937
36	26.87263512974143	23.07301419321289	25.588198399357736	24.466152277687943
37	25.283852854037757	27.107417282283684	26.291723780873035	21.317006082805527
38	23.47620817928653	23.392549646595157	25.79570257886322	27.335539595255092
39	24.035269780521794	20.91426825012715	30.696295350200366	24.35416661915069
40	21.156257626314577	20.839869631359633	32.568805037758096	25.435067704567693
41	22.359179744964635	24.220148751622194	27.330248215870036	26.090423287543135
42	21.82520658046888	19.085275596477583	29.832432050249857	29.257085772803677
43	23.705607721764675	19.00905236412901	31.432755006853704	25.852584907252613
44	25.147782296230574	20.240210377945896	30.382598660277477	24.22940866554605
45	25.06768176002226	21.113151130462153	30.036606309969915	23.78256079954567
46	24.139181523962876	21.500790285757297	29.42271507062395	24.937313119655876
47	22.06486957434046	22.89949344165456	31.251388416896837	23.784248567108147
48	23.837116745619216	20.797128058223418	29.625064716762957	25.740690479394413
49	22.712653010954522	19.291776238513485	32.74332932678601	25.252241423745975
50	21.944308231972247	19.744006714577978	33.65527119459732	24.656413858852456
51	21.715638534926526	18.056512844139228	32.33466149996921	27.89318712096504
52	19.656060339971127	19.92345746027474	34.202381576876675	26.21810062287746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	493.0
1	4020.5
2	7548.0
3	56013.5
4	104479.0
5	59616.5
6	14754.0
7	13250.5
8	11747.0
9	11326.0
10	10905.0
11	10689.5
12	10474.0
13	10124.0
14	9120.0
15	8466.0
16	7901.5
17	7337.0
18	7132.0
19	6927.0
20	6468.0
21	6009.0
22	6019.0
23	6029.0
24	6226.5
25	6424.0
26	7834.0
27	9244.0
28	9789.0
29	10334.0
30	11369.5
31	12405.0
32	14587.5
33	16770.0
34	19723.0
35	22676.0
36	25194.5
37	27713.0
38	30371.5
39	36024.5
40	39019.0
41	42849.5
42	46680.0
43	54775.5
44	62871.0
45	71598.0
46	80325.0
47	91672.5
48	103020.0
49	130632.5
50	158245.0
51	173785.5
52	189326.0
53	176973.5
54	164621.0
55	159727.0
56	154833.0
57	147267.0
58	139701.0
59	136669.5
60	133638.0
61	134264.0
62	134890.0
63	134917.0
64	115933.0
65	96922.0
66	81706.0
67	66490.0
68	55563.0
69	44636.0
70	38031.5
71	31427.0
72	31214.0
73	31001.0
74	24343.5
75	17686.0
76	13428.0
77	9170.0
78	7126.5
79	5083.0
80	3623.0
81	2163.0
82	1758.0
83	1353.0
84	872.0
85	391.0
86	248.5
87	106.0
88	96.0
89	58.5
90	31.0
91	33.0
92	35.0
93	20.0
94	5.0
95	6.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2192245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.682287698170587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44542184203641	19.37611029257022
2	9.438835203293056	4.848217624531855
3	3.3063582373738782	2.547445304563564
4	1.6193178118700982	1.6635114367687973
5	0.9591065630370268	1.231602534256025
6	0.702081277048236	1.0818632006791082
7	0.5657698393596765	1.0171184649768077
8	0.45237688039100393	0.9294458552162134
9	0.39389495518462325	0.9104511205718553
>10	6.065127235347113	37.5827923114039
>50	0.9247290508633673	15.356973320744618
>100	0.11607155732089303	4.9371157662858565
>500	0.006080731044938787	1.0465491210224471
>1k	0.004113435706870355	2.1578157189604363
>5k	1.788450307334937E-4	0.24205898037253878
>10k+	5.365350922004811E-4	5.070928947075717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78863	3.597362521068585	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	18733	0.8545121553476002	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	12806	0.5841500379747702	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	5270	0.24039283930400115	RNA PCR Primer, Index 34 (96% over 26bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4772	0.21767640021986595	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4303	0.1962828059819956	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT	3886	0.17726120939949688	RNA PCR Primer, Index 34 (96% over 27bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3400	0.15509215438967816	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3380	0.15417984759915065	TruSeq Adapter, Index 18 (95% over 24bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2782	0.12690187456237784	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2469	0.11262427329062218	RNA PCR Primer, Index 34 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18560881653282366	0.0
2	0.0	0.0	0.0	0.6925777000289658	0.0
3	0.0	0.0	0.0	1.1284322692034878	0.0
4	0.0	0.0	0.0	1.410928066890334	0.0
5	0.0	0.0	0.0	2.962625071559064	0.0
6	0.0	0.0	0.0	3.41353270277729	0.0
7	4.561533952637593E-5	0.0	0.0	4.777613815974036	0.0
8	4.561533952637593E-5	0.0	0.0	6.384870304185892	0.0
9	4.561533952637593E-5	0.0	0.0	7.693848087234775	0.0
10	4.561533952637593E-5	0.0	0.0	8.65327552349304	0.0
11	4.561533952637593E-5	0.0	0.0	9.4335259060917	0.0
12	4.561533952637593E-5	0.0	0.0	9.98132051846395	0.0
13	4.561533952637593E-5	0.0	0.0	10.267556773991958	0.0
14	4.561533952637593E-5	0.0	0.0	10.457270971082156	0.0
15	4.561533952637593E-5	0.0	0.0	10.989966906071174	0.0
16	4.561533952637593E-5	0.0	0.0	11.575850326947947	0.0
17	4.561533952637593E-5	0.0	0.0	12.332973732406733	0.0
18	4.561533952637593E-5	0.0	0.0	12.765635227814409	0.0
19	4.561533952637593E-5	0.0	0.0	13.20326879523046	0.0
20	4.561533952637593E-5	0.0	0.0	13.735326115466108	0.0
21	4.561533952637593E-5	0.0	0.0	14.279380269997194	0.0
22	4.561533952637593E-5	0.0	0.0	14.78000862129917	0.0
23	4.561533952637593E-5	0.0	0.0	15.251078232588055	0.0
24	9.123067905275186E-5	0.0	0.0	15.616548332873379	0.0
25	9.123067905275186E-5	0.0	0.0	15.984755353530286	0.0
26	9.123067905275186E-5	0.0	0.0	16.32518263241563	0.0
27	9.123067905275186E-5	0.0	0.0	16.702239028940653	0.0
28	9.123067905275186E-5	0.0	0.0	17.080527039632887	0.0
29	1.8246135810550372E-4	0.0	0.0	17.46314850758013	0.0
30	1.8246135810550372E-4	0.0	0.0	17.822414921689866	0.0
31	1.8246135810550372E-4	0.0	0.0	18.19440801552746	0.0
32	1.8246135810550372E-4	0.0	0.0	18.594500158513306	0.0
33	1.8246135810550372E-4	0.0	0.0	18.975342628219018	0.0
34	1.8246135810550372E-4	0.0	0.0	19.43181533085946	0.0
35	1.8246135810550372E-4	0.0	0.0	19.799155660065367	0.0
36	1.8246135810550372E-4	0.0	0.0	20.203444414287638	0.0
37	1.8246135810550372E-4	0.0	0.0	20.682679171351744	0.0
38	1.8246135810550372E-4	0.0	0.0	21.202876503310534	0.0
39	1.8246135810550372E-4	0.0	0.0	21.805044600398222	0.0
40	1.8246135810550372E-4	0.0	0.0	22.366250122591225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18760	0.0	45.031452	1
CATGCGG	395	0.0	44.835445	1
ACGGGTA	160	0.0	44.5625	4
ACGTAGG	135	0.0	44.2963	1
ATAACGG	130	0.0	44.23077	1
ACCGGTA	215	0.0	43.860466	41
CACGACG	380	0.0	43.57895	25
CCGGTAT	220	0.0	42.863636	42
GCACTTA	4685	0.0	42.75987	35
CACTTAC	4700	0.0	42.623405	36
ACTTACT	4720	0.0	42.34534	37
ACCCGCA	4795	0.0	42.30657	31
ACGGGAT	310	0.0	42.29032	4
CGCACTT	4770	0.0	42.190777	34
CCGCACT	4780	0.0	42.150627	33
ATTACGG	175	0.0	42.057144	1
CCCGCAC	4860	0.0	41.930042	32
CTTACTG	4830	0.0	41.190475	38
TTATGAC	5200	0.0	41.178844	26
CCCAACT	1830	0.0	41.098362	13
>>END_MODULE