Basic Statistics
Measure | Value |
---|---|
Filename | SRR1043257_1.fastq.gz |
File type | Conventional base calls |
Encoding | Illumina 1.5 |
Total Sequences | 2142707 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 75111 | 3.505425613487985 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 17815 | 0.8314249218395235 | No Hit |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 10551 | 0.4924145018427624 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 4929 | 0.23003611786399167 | RNA PCR Primer, Index 34 (96% over 25bp) |
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 4617 | 0.21547509762184003 | No Hit |
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 4197 | 0.19587372421894358 | No Hit |
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT | 3670 | 0.17127866759197594 | RNA PCR Primer, Index 34 (96% over 26bp) |
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC | 3388 | 0.1581177454500312 | TruSeq Adapter, Index 18 (95% over 23bp) |
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 3262 | 0.15223733342916226 | No Hit |
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 2630 | 0.1227419334514705 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG | 2373 | 0.11074775972636482 | RNA PCR Primer, Index 34 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAACGTT | 20 | 7.0346525E-4 | 45.000004 | 35 |
TATAACG | 20 | 7.0346525E-4 | 45.000004 | 29 |
TATCGCT | 20 | 7.0346525E-4 | 45.000004 | 20 |
GCGTATC | 20 | 7.0346525E-4 | 45.000004 | 33 |
ATCGAAT | 35 | 1.2125929E-7 | 45.0 | 13 |
CGTTTTT | 18725 | 0.0 | 44.050735 | 1 |
CCGGTAT | 190 | 0.0 | 43.81579 | 42 |
ACGGGTA | 155 | 0.0 | 43.548386 | 4 |
ACATACG | 185 | 0.0 | 42.567566 | 16 |
ACGGGAT | 360 | 0.0 | 42.5 | 4 |
CACGACG | 360 | 0.0 | 41.875 | 25 |
ACGTAGG | 125 | 0.0 | 41.399998 | 1 |
GCACTTA | 4415 | 0.0 | 41.381653 | 35 |
CACTTAC | 4410 | 0.0 | 41.32653 | 36 |
CGATAAG | 60 | 3.6379788E-12 | 41.249996 | 9 |
TAGTAGG | 585 | 0.0 | 41.153847 | 1 |
ACCCGCA | 4510 | 0.0 | 40.958984 | 31 |
CGCACTT | 4475 | 0.0 | 40.877094 | 34 |
ACTTACT | 4440 | 0.0 | 40.79392 | 37 |
CCGCACT | 4490 | 0.0 | 40.64031 | 33 |