##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043257_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2142707
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.388808175826185	33.0	31.0	34.0	31.0	34.0
2	32.68744956729968	34.0	31.0	34.0	31.0	34.0
3	32.817349735638146	34.0	31.0	34.0	31.0	34.0
4	36.25939244143039	37.0	37.0	37.0	35.0	37.0
5	36.25589919667038	37.0	37.0	37.0	35.0	37.0
6	36.35887267834566	37.0	37.0	37.0	35.0	37.0
7	36.32164173636433	37.0	37.0	37.0	35.0	37.0
8	36.236378562257926	37.0	37.0	37.0	35.0	37.0
9	37.84463344731687	39.0	38.0	39.0	35.0	39.0
10	37.599994772967094	39.0	37.0	39.0	35.0	39.0
11	37.304663213402485	39.0	37.0	39.0	35.0	39.0
12	37.31074103925548	39.0	37.0	39.0	35.0	39.0
13	37.32779050052107	39.0	37.0	39.0	35.0	39.0
14	38.633200432910336	40.0	38.0	41.0	35.0	41.0
15	38.703899319879014	40.0	38.0	41.0	35.0	41.0
16	38.620378801208005	40.0	38.0	41.0	34.0	41.0
17	38.53846839535223	40.0	37.0	41.0	34.0	41.0
18	38.402285520138776	40.0	37.0	41.0	34.0	41.0
19	38.23996841378686	40.0	37.0	41.0	34.0	41.0
20	37.98308914844633	40.0	35.0	41.0	34.0	41.0
21	37.936570422367595	40.0	35.0	41.0	34.0	41.0
22	37.916967182167234	40.0	35.0	41.0	34.0	41.0
23	37.88133328541886	40.0	35.0	41.0	34.0	41.0
24	37.79525898781308	40.0	35.0	41.0	34.0	41.0
25	37.749222828879546	40.0	35.0	41.0	34.0	41.0
26	37.69509503632555	40.0	35.0	41.0	34.0	41.0
27	37.67649566646303	40.0	35.0	41.0	34.0	41.0
28	37.5727287958643	39.0	35.0	41.0	34.0	41.0
29	37.53810343644745	39.0	35.0	41.0	34.0	41.0
30	37.4071480608408	39.0	35.0	41.0	34.0	41.0
31	37.3011358995887	39.0	35.0	41.0	33.0	41.0
32	37.08538031564745	39.0	35.0	41.0	33.0	41.0
33	36.722815111912176	39.0	35.0	41.0	32.0	41.0
34	36.4888209167189	39.0	35.0	41.0	31.0	41.0
35	36.343191579623344	39.0	35.0	41.0	31.0	41.0
36	36.286625282878155	39.0	35.0	41.0	31.0	41.0
37	36.168503206458	38.0	35.0	41.0	31.0	41.0
38	36.07270709434374	38.0	35.0	41.0	31.0	41.0
39	35.986904415769395	38.0	35.0	41.0	31.0	41.0
40	35.86906422576675	38.0	35.0	40.0	31.0	41.0
41	35.77568374957472	37.0	35.0	40.0	31.0	41.0
42	35.65698436603791	37.0	35.0	40.0	30.0	41.0
43	35.52730354640182	37.0	35.0	40.0	30.0	41.0
44	35.3807800133196	37.0	35.0	40.0	30.0	41.0
45	35.28033277531646	36.0	35.0	40.0	29.0	41.0
46	35.162674131367474	36.0	35.0	40.0	29.0	41.0
47	35.01633307773765	36.0	35.0	40.0	29.0	41.0
48	34.92160757397068	36.0	35.0	40.0	28.0	41.0
49	34.848500518269645	35.0	35.0	40.0	28.0	41.0
50	34.76635629603114	35.0	35.0	40.0	28.0	41.0
51	34.3719439942092	35.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	3.0
13	5.0
14	10.0
15	33.0
16	95.0
17	182.0
18	377.0
19	731.0
20	1310.0
21	2216.0
22	3529.0
23	5329.0
24	8629.0
25	14440.0
26	22452.0
27	29896.0
28	32738.0
29	32878.0
30	35246.0
31	39527.0
32	47023.0
33	63852.0
34	153928.0
35	224326.0
36	190670.0
37	226906.0
38	346750.0
39	659498.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.786337096019196	18.063925679059246	23.738476609261088	27.41126061566047
2	32.71431884994075	20.76322147638478	25.080890667739453	21.441569005935015
3	27.343962566977194	21.44609598979235	31.327568351622503	19.882373091607953
4	24.07408012388068	24.031610481507737	29.40882724516231	22.485482149449272
5	22.944154287077048	32.71469220943414	26.00448871450926	18.336664788979547
6	82.28707891466263	2.057957527557431	13.291784644377417	2.3631789134025323
7	82.6904938472689	1.989492730457314	12.080606447825112	3.239406974448676
8	74.63414269893177	3.4516618464400404	16.19507473490309	5.719120719725095
9	45.98160177756455	15.755490601374802	21.34169534145359	16.921212279607058
10	29.505900713443324	15.938390083198495	30.756655016294808	23.799054187063373
11	25.690120021076147	13.466470217346563	37.912929765945606	22.930479995631696
12	22.236358027485792	15.494418975622892	39.184032161186764	23.085190835704555
13	18.784882860792447	18.996531023607055	40.916886909876155	21.301699205724347
14	17.739476279304636	17.647489834121043	41.379992691487914	23.2330411950864
15	22.145444990845693	14.486021653917216	40.09236913866431	23.276164216572777
16	22.297962343894895	17.456889812746212	35.35425048781751	24.890897355541377
17	22.766388498287444	19.257089280055556	35.50914800763707	22.467374214019927
18	23.804654579464202	23.66567150805033	31.675166039967202	20.85450787251827
19	25.530322157905864	20.244438460321454	30.39244283049432	23.83279655127836
20	24.82145248977112	23.043047882888327	32.52488557698276	19.610614050357793
21	22.5007898886782	18.576128234051602	33.18157825591646	25.741503621353733
22	23.605047260311373	20.343145376386037	31.219620788096552	24.832186575206038
23	24.04029108972902	18.985983617918826	34.11679711691799	22.856928175434156
24	26.687689917473552	17.168096244610208	30.63167292588301	25.512540912033238
25	21.279437645931058	19.189977911118973	32.551394101013344	26.979190341936626
26	20.178680519548404	18.903611179689992	33.82683680036514	27.090871500396464
27	23.22487395616853	19.830802811583663	33.16365700023381	23.78066623201399
28	24.546519892827156	20.87088902029069	30.558214445558818	24.024376641323336
29	25.5080139281759	18.171033183725076	33.33218214156205	22.988770746536975
30	28.090681553754198	19.26983017276744	30.25719335401434	22.382294919464023
31	30.416197828261165	19.08450385423672	27.71811544928915	22.781182868212966
32	30.324491402697618	18.750580457337378	27.549683647834257	23.375244492130747
33	24.924079680516282	20.043617722815114	31.280898414948943	23.751404181719664
34	25.913715687679183	22.07824028203576	29.780599960703913	22.227444069581143
35	27.778179657788023	23.759244731080827	27.355070011905504	21.10750559922565
36	26.990717816294996	22.93314018202209	25.45014320670068	24.625998794982234
37	25.39208580547877	27.076497159901002	26.14683202136363	21.384585013256597
38	23.60803413625848	23.37855805763457	25.71457506789309	27.298832738213857
39	24.088174444756095	20.854881232011657	30.56330146865624	24.493642854576013
40	21.231834310524025	20.780629362764017	32.40326372201145	25.5842726047005
41	22.433398500121577	24.19406853106841	27.126807351635108	26.24572561717491
42	21.938510491635114	18.995084255570173	29.755258185090167	29.311147067704546
43	23.86957246137713	18.849194033528615	31.311233873786758	25.9699996313075
44	25.169983576849287	20.12953707623114	30.389969323850625	24.31051002306895
45	25.197285489803317	21.036427285671817	29.971293321952093	23.794993902572774
46	24.313170209459344	21.4599569609844	29.401733414787927	24.825139414768326
47	22.27000705182743	22.686442896765634	31.227041308027648	23.816508743379288
48	23.931363457532925	20.76429488492827	29.507814180846935	25.796527476691868
49	22.83597337386773	19.175323551003473	32.70932516671668	25.279377908412116
50	22.09942843328556	19.742783311017327	33.54784391893059	24.609944336766528
51	21.69405336333899	18.093561088846958	32.17490772186771	28.03747782594634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	489.0
1	3961.5
2	7434.0
3	53624.5
4	99815.0
5	57479.5
6	15144.0
7	13420.5
8	11697.0
9	11268.0
10	10839.0
11	10508.0
12	10177.0
13	9685.5
14	9194.0
15	8603.5
16	8013.0
17	7408.5
18	6804.0
19	6490.0
20	6176.0
21	5912.5
22	5649.0
23	5775.5
24	5902.0
25	6206.0
26	7637.5
27	8765.0
28	9776.5
29	10788.0
30	11988.0
31	13188.0
32	15088.0
33	16988.0
34	20831.5
35	24675.0
36	26840.0
37	29005.0
38	31586.0
39	34167.0
40	38352.5
41	42538.0
42	47910.5
43	53283.0
44	60324.0
45	67365.0
46	74639.0
47	81913.0
48	104275.0
49	126637.0
50	151620.5
51	176604.0
52	177933.0
53	179262.0
54	171461.5
55	163661.0
56	155344.5
57	147028.0
58	141124.0
59	135220.0
60	134265.5
61	133311.0
62	132631.0
63	131951.0
64	125692.5
65	119434.0
66	99659.5
67	79885.0
68	65904.5
69	51924.0
70	43946.5
71	35969.0
72	33635.0
73	31301.0
74	26164.5
75	16177.5
76	11327.0
77	8977.5
78	6628.0
79	4566.5
80	2505.0
81	2052.5
82	1600.0
83	1078.0
84	556.0
85	349.0
86	142.0
87	131.5
88	121.0
89	85.5
90	50.0
91	39.0
92	28.0
93	19.0
94	10.0
95	8.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2142707.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.44621991443957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81170540247746	19.036751078457083
2	9.591209081674558	4.881200310753217
3	3.4047137013779283	2.5991128077290493
4	1.6580909763251688	1.6876859048687225
5	1.0223644117885506	1.3007654827534056
6	0.7131542609296293	1.0888248093920982
7	0.5631781473137192	1.0031528491286081
8	0.476995938982859	0.9710194849321945
9	0.4209424940097403	0.9640255746514063
>10	6.316492713550802	38.67091565271533
>50	0.8973503781825487	14.720845809228948
>100	0.11283506651492546	4.8072352113144685
>500	0.005762546322637119	0.9446133449127724
>1k	0.0046472147763202565	2.4292219800370725
>5k	0.0	0.0
>10k+	5.576657731584309E-4	4.894629699125636
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75111	3.505425613487985	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17815	0.8314249218395235	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10551	0.4924145018427624	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	4929	0.23003611786399167	RNA PCR Primer, Index 34 (96% over 25bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4617	0.21547509762184003	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4197	0.19587372421894358	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3670	0.17127866759197594	RNA PCR Primer, Index 34 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3388	0.1581177454500312	TruSeq Adapter, Index 18 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3262	0.15223733342916226	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2630	0.1227419334514705	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	2373	0.11074775972636482	RNA PCR Primer, Index 34 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17813914828298968	0.0
2	0.0	0.0	0.0	0.6774141308167659	0.0
3	0.0	0.0	0.0	1.1126579602344138	0.0
4	0.0	0.0	0.0	1.397951283119904	0.0
5	0.0	0.0	0.0	2.9525735436529588	0.0
6	0.0	0.0	0.0	3.3976647297087283	0.0
7	0.0	0.0	0.0	4.76257369766375	0.0
8	0.0	0.0	0.0	6.40857569420364	0.0
9	0.0	0.0	0.0	7.768490978934591	0.0
10	0.0	0.0	0.0	8.760227133247803	0.0
11	0.0	0.0	0.0	9.558796419669138	0.0
12	0.0	0.0	0.0	10.109408332543834	0.0
13	0.0	0.0	0.0	10.401375456373643	0.0
14	0.0	0.0	0.0	10.59794922964269	0.0
15	0.0	0.0	0.0	11.142167361193108	0.0
16	0.0	0.0	0.0	11.74028926960149	0.0
17	0.0	0.0	0.0	12.504369472821063	0.0
18	0.0	0.0	0.0	12.946100423436336	0.0
19	0.0	0.0	0.0	13.39375845600915	0.0
20	0.0	0.0	0.0	13.938629966672998	0.0
21	4.666993667356293E-5	0.0	0.0	14.48644168334728	0.0
22	4.666993667356293E-5	0.0	0.0	15.008491594977755	0.0
23	4.666993667356293E-5	0.0	0.0	15.493718926572788	0.0
24	9.333987334712586E-5	0.0	0.0	15.878699234193009	0.0
25	9.333987334712586E-5	0.0	0.0	16.258079149412403	0.0
26	9.333987334712586E-5	0.0	0.0	16.612163958954724	0.0
27	9.333987334712586E-5	0.0	0.0	16.995557488728043	0.0
28	9.333987334712586E-5	0.0	0.0	17.37904435837471	0.0
29	9.333987334712586E-5	0.0	0.0	17.765378094158464	0.0
30	9.333987334712586E-5	0.0	0.0	18.13547069197982	0.0
31	9.333987334712586E-5	0.0	0.0	18.51368385878237	0.0
32	9.333987334712586E-5	0.0	0.0	18.93021304359392	0.0
33	9.333987334712586E-5	0.0	0.0	19.31267317463377	0.0
34	9.333987334712586E-5	0.0	0.0	19.77535892681547	0.0
35	9.333987334712586E-5	0.0	0.0	20.149371799317404	0.0
36	9.333987334712586E-5	0.0	0.0	20.557547065464387	0.0
37	9.333987334712586E-5	0.0	0.0	21.045434583449815	0.0
38	9.333987334712586E-5	0.0	0.0	21.57154477957089	0.0
39	9.333987334712586E-5	0.0	0.0	22.195381823086404	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTT	20	7.0346525E-4	45.000004	35
TATAACG	20	7.0346525E-4	45.000004	29
TATCGCT	20	7.0346525E-4	45.000004	20
GCGTATC	20	7.0346525E-4	45.000004	33
ATCGAAT	35	1.2125929E-7	45.0	13
CGTTTTT	18725	0.0	44.050735	1
CCGGTAT	190	0.0	43.81579	42
ACGGGTA	155	0.0	43.548386	4
ACATACG	185	0.0	42.567566	16
ACGGGAT	360	0.0	42.5	4
CACGACG	360	0.0	41.875	25
ACGTAGG	125	0.0	41.399998	1
GCACTTA	4415	0.0	41.381653	35
CACTTAC	4410	0.0	41.32653	36
CGATAAG	60	3.6379788E-12	41.249996	9
TAGTAGG	585	0.0	41.153847	1
ACCCGCA	4510	0.0	40.958984	31
CGCACTT	4475	0.0	40.877094	34
ACTTACT	4440	0.0	40.79392	37
CCGCACT	4490	0.0	40.64031	33
>>END_MODULE