FastQCFastQC Report
Thu 26 May 2016
SRR1043256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043256_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1153419
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT821457.121869849551637No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT86730.7519383675836795Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT76280.6613381607204321Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT52610.45612218976798546Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC40010.34688174895679713No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG28690.24873874975182478No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT26810.2324393823926951Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT25530.22134194078647917No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC19070.16533454018010801No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC18530.16065280700248563No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC16920.14669430623216714No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT16130.13984510399083072No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC15640.1355968646259512No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT14850.12874766238461477No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC14100.12224525519347262No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT13770.11938419602937006Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG13080.11340198141351929No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC11840.10265133485749757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTC301.86141E-646.024
CACGGTG206.311464E-446.03
TTTACGG800.046.01
GCCGGTG405.6115823E-946.046
CAATTGT405.6115823E-946.018
TAACGGT206.311464E-446.033
TGCGGCA206.311464E-446.03
GTGGCGT405.6115823E-946.034
ATCGTCC301.86141E-646.034
CTTGGAC206.311464E-446.035
AACGGCC405.6115823E-946.05
GCCGAAT206.311464E-446.039
ACGGCGT206.311464E-446.03
CTCGGTA301.86141E-646.020
CTACGTC405.6115823E-946.013
AACGCTA405.6115823E-946.09
ACTACGG900.046.01
CTTTGCG253.4171637E-546.038
TTGCGAT253.4171637E-546.040
TCCGTTC206.311464E-446.021
Produced by FastQC (version 0.11.5)