##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043256_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1153419
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28308879947357	33.0	31.0	34.0	31.0	34.0
2	32.61786393322808	34.0	31.0	34.0	31.0	34.0
3	32.681628272119674	34.0	31.0	34.0	31.0	34.0
4	36.20474259570893	37.0	37.0	37.0	35.0	37.0
5	36.10874799183991	37.0	35.0	37.0	35.0	37.0
6	36.33210221090514	37.0	37.0	37.0	35.0	37.0
7	36.25923970387171	37.0	37.0	37.0	35.0	37.0
8	36.239317195225674	37.0	37.0	37.0	35.0	37.0
9	37.80875813559513	39.0	38.0	39.0	35.0	39.0
10	37.64095268068239	39.0	37.0	39.0	35.0	39.0
11	37.309609083949546	39.0	37.0	39.0	35.0	39.0
12	37.33825088714509	39.0	37.0	39.0	34.0	39.0
13	37.33153693497333	39.0	37.0	39.0	34.0	39.0
14	38.74843140263859	40.0	38.0	41.0	35.0	41.0
15	38.84947447545081	41.0	38.0	41.0	35.0	41.0
16	38.88309885653002	41.0	38.0	41.0	35.0	41.0
17	38.83832328061182	41.0	38.0	41.0	35.0	41.0
18	38.55723895652837	40.0	38.0	41.0	35.0	41.0
19	38.258047595886666	40.0	37.0	41.0	35.0	41.0
20	37.94367007999695	40.0	35.0	41.0	34.0	41.0
21	37.8783729069835	40.0	35.0	41.0	34.0	41.0
22	37.82261173086277	40.0	35.0	41.0	34.0	41.0
23	37.82326197158188	40.0	35.0	41.0	34.0	41.0
24	37.7211351642378	40.0	35.0	41.0	34.0	41.0
25	37.6274068660218	39.0	35.0	41.0	34.0	41.0
26	37.59578002443171	39.0	35.0	41.0	34.0	41.0
27	37.600733991723736	39.0	35.0	41.0	34.0	41.0
28	37.58144525103193	39.0	35.0	41.0	34.0	41.0
29	37.491868956554384	39.0	35.0	41.0	34.0	41.0
30	37.4357046311878	40.0	35.0	41.0	33.0	41.0
31	37.193926925081	39.0	35.0	41.0	33.0	41.0
32	36.937940158780115	39.0	35.0	41.0	33.0	41.0
33	36.73408102346155	39.0	35.0	41.0	32.0	41.0
34	36.23065425487182	39.0	35.0	41.0	30.0	41.0
35	36.14259085380074	39.0	35.0	41.0	30.0	41.0
36	35.935812571147174	39.0	35.0	41.0	30.0	41.0
37	35.77991779223335	38.0	35.0	41.0	30.0	41.0
38	35.74387624965429	38.0	35.0	41.0	30.0	41.0
39	35.81415426657615	38.0	35.0	41.0	30.0	41.0
40	35.86031182076938	39.0	35.0	41.0	30.0	41.0
41	35.91857252221439	39.0	35.0	41.0	30.0	41.0
42	35.91627847295736	39.0	35.0	41.0	30.0	41.0
43	35.85228438234501	39.0	35.0	41.0	30.0	41.0
44	35.65963192907348	38.0	35.0	41.0	28.0	41.0
45	35.55194079514903	38.0	35.0	41.0	27.0	41.0
46	35.49274114610562	38.0	35.0	41.0	27.0	41.0
47	35.42928372083345	38.0	35.0	41.0	27.0	41.0
48	35.38576614395983	38.0	35.0	40.0	27.0	41.0
49	35.3269366986325	38.0	35.0	40.0	27.0	41.0
50	35.186108430674366	38.0	35.0	40.0	26.0	41.0
51	35.10203924159391	38.0	35.0	40.0	26.0	41.0
52	34.77538171297681	37.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	7.0
15	13.0
16	36.0
17	70.0
18	182.0
19	340.0
20	754.0
21	1236.0
22	1965.0
23	3635.0
24	6838.0
25	12717.0
26	17716.0
27	18821.0
28	17003.0
29	16233.0
30	17981.0
31	21001.0
32	25728.0
33	34062.0
34	63497.0
35	108833.0
36	121725.0
37	88629.0
38	153437.0
39	420594.0
40	360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.98257875065349	19.321339426522364	25.00886494847059	29.68721687435355
2	34.010103873787415	19.81621596314956	26.660302977495604	19.51337718556743
3	20.576997604513192	20.841342131523756	39.99110470696252	18.590555557000535
4	22.055038108441078	23.839818834265778	36.87255021809074	17.232592839202407
5	19.914098866066883	28.570450113965524	32.92576245059254	18.58968856937505
6	71.3012357174626	1.6572468461157654	23.046958650759176	3.994558785662452
7	72.17351196746368	1.4414536261323943	19.437168973287243	6.947865433116673
8	60.34424610657532	3.222679702692603	27.31886677781448	9.114207412917596
9	34.01920724385501	17.578694299296266	30.578826948402966	17.82327150844576
10	26.04907670152824	18.99656586201545	42.848695920563124	12.105661515893184
11	23.250700742748297	16.00173050730047	41.39458427509864	19.35298447485259
12	19.0541338403477	19.43560839556137	43.41839348926973	18.091864274821205
13	13.47654234931105	22.315741287424604	43.16896114941751	21.038755213846834
14	11.8935963426994	22.287304093308677	44.52640367464035	21.29269588935157
15	12.928519471241588	18.610409573624157	48.089809514148804	20.371261440985453
16	14.087768625278411	24.463529732040136	39.59541155469088	21.853290087990572
17	15.255947751857738	22.14277725614022	41.785769091717754	20.815505900284283
18	17.018360196944908	27.51107793438464	39.67630150014869	15.794260368521758
19	16.320868652241728	23.45791078523936	37.15232712483495	23.06889343768396
20	14.342662987171185	29.058217352063735	36.23106607399393	20.368053586771158
21	14.9295269108624	23.84285329095498	37.43453159693051	23.793088201252104
22	12.973862924054485	26.23357166823158	33.78590087383683	27.006664533877107
23	12.512712206058682	23.128455487554824	43.49295442506149	20.865877881325
24	17.121531724377697	21.642872191285214	39.4708254329086	21.764770651428492
25	13.238120752302502	24.64707101235544	39.73204880446741	22.382759430874643
26	15.067204545789517	21.47207562906455	39.007854040899275	24.45286578424666
27	20.249362980842175	21.558427596562915	39.1510803966295	19.04112902596541
28	16.59006830995501	21.6257058363006	35.923805659521825	25.860420194222566
29	19.90360831579851	20.957605172101378	37.67364678403945	21.465139728060663
30	22.44015401168179	20.112639032303093	32.36308748165237	25.084119474362744
31	24.3631325650089	18.810163522536044	35.29671351000807	21.529990402446984
32	22.269184051936026	19.46933421419276	35.59582424080061	22.6656574930706
33	23.66607451411846	18.75250884544125	34.573559131590514	23.007857508849778
34	23.30618795077938	18.344677866412813	37.29139193996284	21.057742242844967
35	31.88901864803684	18.07469791983659	32.23642058956892	17.799862842557648
36	25.213734124372845	23.181168335184353	33.952622594217715	17.65247494622509
37	25.36606385017067	19.975481589951265	38.458530681391586	16.199923878486484
38	23.164001980199735	22.975258774131515	33.6192658522185	20.241473393450253
39	25.77831646608908	22.07619260650293	35.73939739158103	16.40609353582696
40	17.803677588109785	20.108910985513504	40.576234655402764	21.511176770973947
41	20.857814896407984	22.819807892881943	33.1179736071627	23.204403603547366
42	21.958542385724527	18.785974567784994	36.75585368370037	22.499629362790106
43	25.44886116840454	20.73149479937473	34.4687403276693	19.350903704551424
44	18.591682640913668	22.611990959053042	36.578121220475815	22.218205179557472
45	21.71535235677581	19.721801010734175	36.349843378685456	22.21300325380456
46	24.96248111050711	18.74999458132734	34.87180287475757	21.41572143340798
47	19.810927338634094	17.8026372029592	38.470581809385834	23.915853649020868
48	20.41833886904932	16.340289175052604	33.91828988424848	29.32308207164959
49	21.92906480645802	15.905061387058822	39.36019781189663	22.80567599458653
50	20.09833373648258	15.991933547132481	39.860276274276735	24.0494564421082
51	17.663832484118956	15.811426723506376	38.41136655456517	28.113374237809502
52	16.311245089598835	15.53381728582588	46.09166313369209	22.06327449088319
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	429.0
1	2041.5
2	3654.0
3	47302.0
4	90950.0
5	50063.5
6	9177.0
7	8769.5
8	8362.0
9	8788.0
10	9214.0
11	9518.0
12	9822.0
13	9481.5
14	8383.0
15	7625.0
16	6919.5
17	6214.0
18	5650.5
19	5087.0
20	4780.0
21	4473.0
22	4193.5
23	3914.0
24	3987.0
25	4060.0
26	4461.0
27	4862.0
28	4930.5
29	4999.0
30	5866.0
31	6733.0
32	8074.0
33	9415.0
34	11134.5
35	12854.0
36	14425.0
37	15996.0
38	18587.5
39	22555.0
40	23931.0
41	29981.0
42	36031.0
43	43072.5
44	50114.0
45	59323.0
46	68532.0
47	87164.5
48	105797.0
49	133956.0
50	162115.0
51	165654.5
52	169194.0
53	145195.5
54	121197.0
55	95269.5
56	69342.0
57	54198.5
58	39055.0
59	30521.0
60	21987.0
61	17804.0
62	13621.0
63	11279.0
64	7396.5
65	5856.0
66	4340.0
67	2824.0
68	2396.5
69	1969.0
70	1754.0
71	1539.0
72	1519.0
73	1499.0
74	1170.5
75	842.0
76	603.0
77	364.0
78	331.0
79	298.0
80	230.5
81	163.0
82	93.5
83	24.0
84	18.5
85	13.0
86	11.0
87	9.0
88	5.5
89	1.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1153419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.579775116485514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86408299200859	23.32611130073054
2	11.769000519194893	7.43324779483952
3	4.658357239279381	4.413296220860854
4	2.361764178934778	2.9833592659572536
5	1.3431376142659075	2.120799190450511
6	0.8297123566210827	1.572127778007781
7	0.5860025833012865	1.2954080858833938
8	0.4545796621652308	1.1484418802964758
9	0.3607994704345103	1.0254569524622008
>10	3.168768656720749	21.876184217446024
>50	0.41124135250862676	8.758726821574896
>100	0.17714910460055722	10.3508114645939
>500	0.00990274497709602	2.1201116578955235
>1k	0.0044012199898204535	2.5670498894726963
>5k	8.252287480913349E-4	1.8730577549595708
>10k+	2.7507624936377834E-4	7.135809724568869
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82145	7.121869849551637	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	8673	0.7519383675836795	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	7628	0.6613381607204321	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	5261	0.45612218976798546	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	4001	0.34688174895679713	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	2869	0.24873874975182478	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	2681	0.2324393823926951	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	2553	0.22134194078647917	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1907	0.16533454018010801	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1853	0.16065280700248563	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	1692	0.14669430623216714	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	1613	0.13984510399083072	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1564	0.1355968646259512	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	1485	0.12874766238461477	No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1410	0.12224525519347262	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1377	0.11938419602937006	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1308	0.11340198141351929	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1184	0.10265133485749757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6823192612571841	0.0
2	0.0	0.0	0.0	2.2969103161990567	0.0
3	0.0	0.0	0.0	3.7875221406964856	0.0
4	0.0	0.0	0.0	4.698812833844422	0.0
5	0.0	0.0	0.0	9.656334775133754	0.0
6	0.0	0.0	0.0	11.050364178152085	0.0
7	0.0	0.0	0.0	15.131361630075453	0.0
8	0.0	0.0	0.0	20.130065483575354	0.0
9	0.0	0.0	0.0	23.873284556609523	0.0
10	0.0	0.0	0.0	26.216231915721867	0.0
11	0.0	0.0	0.0	28.20163357808394	0.0
12	0.0	0.0	0.0	29.57095383377593	0.0
13	0.0	0.0	0.0	30.162759586932417	0.0
14	0.0	0.0	0.0	30.57613928676396	0.0
15	0.0	0.0	0.0	31.910086447336138	0.0
16	0.0	0.0	0.0	33.43312360902673	0.0
17	0.0	0.0	0.0	35.09548568213286	0.0
18	8.669876254856214E-5	0.0	0.0	35.98596867226914	0.0
19	8.669876254856214E-5	0.0	0.0	36.970433121008064	0.0
20	8.669876254856214E-5	0.0	0.0	38.10696719925716	0.0
21	8.669876254856214E-5	0.0	0.0	39.20136567890766	0.0
22	1.7339752509712428E-4	0.0	0.0	40.24721285153097	0.0
23	1.7339752509712428E-4	0.0	0.0	41.06807673534075	0.0
24	2.6009628764568643E-4	0.0	0.0	41.77276427733547	0.0
25	2.6009628764568643E-4	0.0	0.0	42.43661670216981	0.0
26	2.6009628764568643E-4	0.0	0.0	43.12812603225714	0.0
27	3.4679505019424855E-4	0.0	0.0	43.779233738996844	0.0
28	3.4679505019424855E-4	0.0	0.0	44.431988722224965	0.0
29	3.4679505019424855E-4	0.0	0.0	45.1867014502102	0.0
30	3.4679505019424855E-4	0.0	0.0	45.851420862670025	0.0
31	3.4679505019424855E-4	0.0	0.0	46.45328367228215	0.0
32	3.4679505019424855E-4	0.0	0.0	47.036419549183776	0.0
33	3.4679505019424855E-4	0.0	0.0	47.60793779190389	0.0
34	3.4679505019424855E-4	0.0	0.0	48.26762867613591	0.0
35	4.3349381274281073E-4	0.0	0.0	48.80966933958952	0.0
36	4.3349381274281073E-4	0.0	0.0	49.352490291906065	0.0
37	4.3349381274281073E-4	0.0	0.0	49.88464729642914	0.0
38	4.3349381274281073E-4	0.0	0.0	50.440733159415615	0.0
39	4.3349381274281073E-4	0.0	0.0	50.98277382286923	0.0
40	4.3349381274281073E-4	0.0	0.0	51.55385857177661	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTC	30	1.86141E-6	46.0	24
CACGGTG	20	6.311464E-4	46.0	3
TTTACGG	80	0.0	46.0	1
GCCGGTG	40	5.6115823E-9	46.0	46
CAATTGT	40	5.6115823E-9	46.0	18
TAACGGT	20	6.311464E-4	46.0	33
TGCGGCA	20	6.311464E-4	46.0	3
GTGGCGT	40	5.6115823E-9	46.0	34
ATCGTCC	30	1.86141E-6	46.0	34
CTTGGAC	20	6.311464E-4	46.0	35
AACGGCC	40	5.6115823E-9	46.0	5
GCCGAAT	20	6.311464E-4	46.0	39
ACGGCGT	20	6.311464E-4	46.0	3
CTCGGTA	30	1.86141E-6	46.0	20
CTACGTC	40	5.6115823E-9	46.0	13
AACGCTA	40	5.6115823E-9	46.0	9
ACTACGG	90	0.0	46.0	1
CTTTGCG	25	3.4171637E-5	46.0	38
TTGCGAT	25	3.4171637E-5	46.0	40
TCCGTTC	20	6.311464E-4	46.0	21
>>END_MODULE