FastQCFastQC Report
Thu 26 May 2016
SRR1043255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043255_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1126366
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT747626.637451769673445No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC83010.7369718191067557No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT74850.6645264505498213Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC52370.46494656266258033No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG41360.3671985837640696No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC26470.23500354236544782No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC25490.22630299565150228No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC24560.21804635438214576No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG19070.1693055365662671No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG18790.16681966607656834No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT17380.1543015325391569No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG16590.14728782651464975No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT15940.14151705573499201No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC14230.12633548953004617No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC13680.12145252963956653No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT13330.11834519152744312No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG13260.11772372390501844No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG11400.10121044136630546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT253.890755E-545.00000427
CGGCGTT302.1654632E-645.00000442
CCGGCGT302.1654632E-645.00000441
CCTACGG253.890755E-545.0000041
GCCATTA253.890755E-545.0000048
CCCCGTC253.890755E-545.00000411
CGCAGTA253.890755E-545.00000436
TCATCGG502.1827873E-1145.0000041
GCGGAAT253.890755E-545.0000044
AATAGGC302.1654632E-645.0000042
TCGCGCA253.890755E-545.00000433
AGCGTAG207.0332276E-445.036
ATTAGCG207.0332276E-445.011
TCCGATA207.0332276E-445.044
CGGCATC453.8562575E-1045.022
ATTACGG1750.045.01
TTCACGT207.0332276E-445.030
TTCACGC207.0332276E-445.01
TAAACGG551.8189894E-1245.01
TCGGCAT453.8562575E-1045.021
Produced by FastQC (version 0.11.5)