##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043255_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1126366
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21312521862343	33.0	31.0	34.0	31.0	34.0
2	32.521338534721394	34.0	31.0	34.0	31.0	34.0
3	32.57562373154019	34.0	31.0	34.0	31.0	34.0
4	36.15548676007621	37.0	35.0	37.0	35.0	37.0
5	36.112789270982965	37.0	35.0	37.0	35.0	37.0
6	36.33104869997852	37.0	37.0	37.0	35.0	37.0
7	36.27129636370416	37.0	37.0	37.0	35.0	37.0
8	36.17602804061912	37.0	37.0	37.0	35.0	37.0
9	37.74407164278751	39.0	38.0	39.0	35.0	39.0
10	37.57032705177536	39.0	37.0	39.0	35.0	39.0
11	37.29277339692427	39.0	37.0	39.0	34.0	39.0
12	37.3161103939572	39.0	37.0	39.0	34.0	39.0
13	37.31883508557609	39.0	37.0	39.0	34.0	39.0
14	38.72861751863959	40.0	38.0	41.0	35.0	41.0
15	38.84979837814707	40.0	38.0	41.0	35.0	41.0
16	38.90532029553449	41.0	38.0	41.0	35.0	41.0
17	38.84124787147339	41.0	38.0	41.0	35.0	41.0
18	38.59248858719102	40.0	38.0	41.0	35.0	41.0
19	38.31712516180353	40.0	37.0	41.0	35.0	41.0
20	38.0255139093332	40.0	35.0	41.0	34.0	41.0
21	37.96564970888681	40.0	35.0	41.0	34.0	41.0
22	37.925641398976886	40.0	35.0	41.0	34.0	41.0
23	37.88625988355472	40.0	35.0	41.0	34.0	41.0
24	37.795985496721315	40.0	35.0	41.0	34.0	41.0
25	37.758622863261145	40.0	35.0	41.0	34.0	41.0
26	37.76564811082721	40.0	35.0	41.0	34.0	41.0
27	37.73728255291797	40.0	35.0	41.0	34.0	41.0
28	37.669801822853316	40.0	35.0	41.0	34.0	41.0
29	37.62404138619241	40.0	35.0	41.0	34.0	41.0
30	37.51916073461024	40.0	35.0	41.0	34.0	41.0
31	37.28170594637977	39.0	35.0	41.0	33.0	41.0
32	37.04088369144665	39.0	35.0	41.0	33.0	41.0
33	36.81357125481416	39.0	35.0	41.0	32.0	41.0
34	36.28665016522161	39.0	35.0	41.0	30.0	41.0
35	36.19862904242493	39.0	35.0	41.0	30.0	41.0
36	36.00019176715206	39.0	35.0	41.0	30.0	41.0
37	35.81812217343208	38.0	35.0	41.0	30.0	41.0
38	35.77391895707079	38.0	35.0	41.0	30.0	41.0
39	35.78062192928409	38.0	35.0	41.0	29.0	41.0
40	35.794599623923304	38.0	35.0	40.0	29.0	41.0
41	35.80856666483186	39.0	35.0	41.0	29.0	41.0
42	35.792439580029935	39.0	35.0	41.0	29.0	41.0
43	35.712697293774845	38.0	35.0	41.0	29.0	41.0
44	35.57962331959594	38.0	35.0	40.0	28.0	41.0
45	35.501051168092786	38.0	35.0	40.0	27.0	41.0
46	35.38862678738527	38.0	35.0	40.0	27.0	41.0
47	35.2558289223929	38.0	35.0	40.0	26.0	41.0
48	35.231335995582256	38.0	35.0	40.0	26.0	41.0
49	35.16225720591708	38.0	35.0	40.0	26.0	41.0
50	35.060401326034345	38.0	35.0	40.0	26.0	41.0
51	34.62134954357642	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	8.0
16	22.0
17	66.0
18	159.0
19	307.0
20	576.0
21	1022.0
22	1785.0
23	2916.0
24	5416.0
25	10473.0
26	16258.0
27	18723.0
28	18050.0
29	16957.0
30	17913.0
31	20555.0
32	25098.0
33	33725.0
34	62750.0
35	107353.0
36	113281.0
37	88939.0
38	162192.0
39	401787.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.257983639420935	19.439596010532988	25.111731000403065	29.190689349643012
2	33.571947306648106	19.88785172847902	26.792800919061833	19.74740004581104
3	20.808334058378893	21.00116658350838	39.368908507536624	18.8215908505761
4	22.304828093177527	24.072282011353327	36.21700228877647	17.405887606692673
5	20.21083732996202	28.726896941136363	32.31338659014921	18.748879138752415
6	71.9263543111209	1.6262919867964762	22.42201913054904	4.025334571533587
7	72.85802305822442	1.3939518770985628	18.75615918804367	6.991865876633351
8	60.84132511102075	3.1768537047460597	26.73314002730906	9.248681156924125
9	34.37186491779759	17.600939659045107	29.978443951610757	18.04875147154655
10	26.43217213587768	19.02729663359867	42.34183205103847	12.198699179485176
11	23.566762491055304	15.935406430946959	41.004877633025146	19.492953444972592
12	19.351436389237602	19.466940585919673	42.91491398000295	18.266709044839775
13	13.649914858935727	22.377095899556625	42.865374132386805	21.107615109120836
14	12.031435607964019	22.272955682256036	44.16406390107656	21.531544808703387
15	13.03359653966828	18.499670622160114	47.87404804477408	20.592684793397527
16	14.277952281940328	24.402814005394337	39.19436488672421	22.124868825941125
17	15.49833713020457	22.01753248943949	41.39169683744005	21.092433542915888
18	17.29926151890238	27.497811546158175	39.345736643329076	15.857190291610365
19	16.613338825923368	23.431548892633476	36.749777603372266	23.205334678070894
20	14.45631348957621	29.049261074996934	35.85513057034747	20.63929486507938
21	14.969379402432248	23.844647299368056	37.04444203749048	24.14153126070922
22	13.207518692858272	26.093649843834065	33.34040622675045	27.35842523655721
23	12.66115987165806	23.170354929037277	43.07214528847639	21.096339910828274
24	17.24013331368312	21.538203390372225	39.08445389864396	22.1372093973007
25	13.286089956550533	24.649536651496938	39.45120857696344	22.61316481498909
26	15.177482274855597	21.366589545494094	38.71228357390049	24.743644605749818
27	20.48295136749511	21.400503921460697	38.82716630296014	19.28937840808405
28	16.652491286136122	21.648647064985983	35.54439675913513	26.15446488974277
29	20.051120150998877	20.957930193205407	37.1751278003775	21.81582185541822
30	22.76400388506045	20.013033063853133	31.83760873463865	25.38535431644776
31	24.65468595465417	18.758822620711207	34.704705220150466	21.881786204484154
32	22.502099672752905	19.296480895197476	35.19424414444328	23.007175287606337
33	23.939199159065524	18.55711198668994	34.18036410900187	23.323324745242665
34	23.680402284870105	18.106991865876633	36.892271251085354	21.320334598167914
35	32.267486767178696	17.826976311429853	31.93464646482582	17.970890456565627
36	25.59478890520488	23.017473893920805	33.45466748818768	17.93306971268664
37	25.86761319144932	19.671847339141983	38.13795870969117	16.322580759717535
38	23.588957763284757	22.747934507966328	33.28269851895387	20.38040920979504
39	26.22966247205615	21.95511938393027	35.318537668928215	16.496680475085363
40	18.003117991842792	19.91421971188761	40.3422155853426	21.740446710927
41	21.24211845883132	22.568419146174513	32.78712248061465	23.402339914379517
42	22.355255751682847	18.484666618133005	36.37485506487234	22.78522256531181
43	25.824376801146343	20.53497708560095	34.13251110207517	19.508135011177536
44	18.971186985402614	22.34176102616734	36.29397549286821	22.393076495561832
45	22.090244201263175	19.414115838013576	36.14127201992957	22.35436794079367
46	25.38899434109339	18.470372862817236	34.58192097417713	21.558711821912237
47	20.209239270361497	17.526807449798735	38.30682034081284	23.957132939026923
48	20.753112221072016	16.020192370863466	33.57958248029504	29.64711292776948
49	22.226256829485266	15.565988319959942	39.15636658066738	23.051388269887408
50	20.38964244304249	15.684244730398467	39.68789896001832	24.23821386654072
51	18.05603152083781	15.618724286777121	37.94379446822791	28.38144972415716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	354.0
1	1846.0
2	3338.0
3	43204.5
4	83071.0
5	46254.5
6	9438.0
7	8879.0
8	8320.0
9	8638.5
10	8957.0
11	9089.5
12	9222.0
13	8716.0
14	8210.0
15	7438.5
16	6667.0
17	6067.5
18	5468.0
19	5015.5
20	4563.0
21	4183.5
22	3804.0
23	3559.0
24	3314.0
25	3509.5
26	4206.0
27	4707.0
28	4854.0
29	5001.0
30	6540.5
31	8080.0
32	8889.5
33	9699.0
34	11389.0
35	13079.0
36	15087.5
37	17096.0
38	19740.0
39	22384.0
40	25539.5
41	28695.0
42	35926.0
43	43157.0
44	48420.5
45	53684.0
46	62712.5
47	71741.0
48	96606.0
49	121471.0
50	148960.0
51	176449.0
52	172825.5
53	169202.0
54	133210.0
55	97218.0
56	74681.5
57	52145.0
58	40646.5
59	29148.0
60	22848.5
61	16549.0
62	13460.0
63	10371.0
64	8577.5
65	6784.0
66	5294.5
67	3805.0
68	2960.5
69	2116.0
70	1913.5
71	1711.0
72	1569.0
73	1427.0
74	1214.5
75	767.5
76	533.0
77	469.0
78	405.0
79	299.0
80	193.0
81	118.0
82	43.0
83	29.0
84	15.0
85	14.0
86	13.0
87	8.5
88	4.0
89	2.5
90	1.0
91	3.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1126366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.16294470169739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18131890827306	22.805453943357946
2	12.007446177883619	7.483747624999898
3	4.702868763656247	4.396657176634789
4	2.47981043266455	3.0911278153527064
5	1.379923610006331	2.1501241575596963
6	0.9148303579175084	1.7105284713130406
7	0.6073901340752164	1.324964561237977
8	0.45037295486754503	1.1227957990141888
9	0.3566865175087226	1.0003861998868806
>10	3.310561222559106	22.488997881396518
>50	0.407009210016104	8.498725495336917
>100	0.1859950902572726	10.607410357872944
>500	0.00975900164905416	2.037509385680784
>1k	0.00487950082452708	2.7139063291163206
>5k	8.610883807988964E-4	1.8804407487232593
>10k+	2.8702946026629877E-4	6.6872240525162105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74762	6.637451769673445	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	8301	0.7369718191067557	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	7485	0.6645264505498213	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	5237	0.46494656266258033	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	4136	0.3671985837640696	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC	2647	0.23500354236544782	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2549	0.22630299565150228	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	2456	0.21804635438214576	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1907	0.1693055365662671	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1879	0.16681966607656834	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	1738	0.1543015325391569	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1659	0.14728782651464975	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	1594	0.14151705573499201	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	1423	0.12633548953004617	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1368	0.12145252963956653	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	1333	0.11834519152744312	No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1326	0.11772372390501844	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	1140	0.10121044136630546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6837919468449865	0.0
2	0.0	0.0	0.0	2.289397939923613	0.0
3	0.0	0.0	0.0	3.8277966486914554	0.0
4	0.0	0.0	0.0	4.7645259178632875	0.0
5	0.0	0.0	0.0	9.772667143717051	0.0
6	0.0	0.0	0.0	11.178870811086272	0.0
7	0.0	0.0	0.0	15.282776646312122	0.0
8	0.0	0.0	0.0	20.39674493015592	0.0
9	0.0	0.0	0.0	24.231821628138633	0.0
10	0.0	0.0	0.0	26.658297569351348	0.0
11	0.0	0.0	0.0	28.700351395549937	0.0
12	0.0	0.0	0.0	30.111881928254228	0.0
13	0.0	0.0	0.0	30.721364103675004	0.0
14	0.0	0.0	0.0	31.16091927490709	0.0
15	0.0	0.0	0.0	32.55371699784972	0.0
16	0.0	0.0	0.0	34.11848369002615	0.0
17	0.0	0.0	0.0	35.854509102725046	0.0
18	0.0	0.0	0.0	36.791771058430385	0.0
19	0.0	0.0	0.0	37.80298766120426	0.0
20	0.0	0.0	0.0	38.99025716330216	0.0
21	0.0	0.0	0.0	40.09735734210727	0.0
22	0.0	0.0	0.0	41.18394908937237	0.0
23	0.0	0.0	0.0	42.03633632407228	0.0
24	3.551243556712472E-4	0.0	0.0	42.748982124815555	0.0
25	3.551243556712472E-4	0.0	0.0	43.43082088770435	0.0
26	3.551243556712472E-4	0.0	0.0	44.11363624257124	0.0
27	3.551243556712472E-4	0.0	0.0	44.76493431087231	0.0
28	3.551243556712472E-4	0.0	0.0	45.42608708004325	0.0
29	3.551243556712472E-4	0.0	0.0	46.1854317335573	0.0
30	3.551243556712472E-4	0.0	0.0	46.85972410388808	0.0
31	3.551243556712472E-4	0.0	0.0	47.46458966268513	0.0
32	3.551243556712472E-4	0.0	0.0	48.063773231791444	0.0
33	3.551243556712472E-4	0.0	0.0	48.62637899226362	0.0
34	3.551243556712472E-4	0.0	0.0	49.29561084052608	0.0
35	3.551243556712472E-4	0.0	0.0	49.836731577480144	0.0
36	3.551243556712472E-4	0.0	0.0	50.391347039949714	0.0
37	3.551243556712472E-4	0.0	0.0	50.93149118492568	0.0
38	3.551243556712472E-4	0.0	0.0	51.4978257511324	0.0
39	3.551243556712472E-4	0.0	0.0	52.04684800500015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTT	25	3.890755E-5	45.000004	27
CGGCGTT	30	2.1654632E-6	45.000004	42
CCGGCGT	30	2.1654632E-6	45.000004	41
CCTACGG	25	3.890755E-5	45.000004	1
GCCATTA	25	3.890755E-5	45.000004	8
CCCCGTC	25	3.890755E-5	45.000004	11
CGCAGTA	25	3.890755E-5	45.000004	36
TCATCGG	50	2.1827873E-11	45.000004	1
GCGGAAT	25	3.890755E-5	45.000004	4
AATAGGC	30	2.1654632E-6	45.000004	2
TCGCGCA	25	3.890755E-5	45.000004	33
AGCGTAG	20	7.0332276E-4	45.0	36
ATTAGCG	20	7.0332276E-4	45.0	11
TCCGATA	20	7.0332276E-4	45.0	44
CGGCATC	45	3.8562575E-10	45.0	22
ATTACGG	175	0.0	45.0	1
TTCACGT	20	7.0332276E-4	45.0	30
TTCACGC	20	7.0332276E-4	45.0	1
TAAACGG	55	1.8189894E-12	45.0	1
TCGGCAT	45	3.8562575E-10	45.0	21
>>END_MODULE