FastQCFastQC Report
Thu 26 May 2016
SRR1043254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043254_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences909118
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT632896.961582544840164No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT76950.846424776541659No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT70010.7700870514058681Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT49000.5389839382786393No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC32320.3555094058197066No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT27920.30711084809672673No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG26280.28907138567270696No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC23400.2573923297085747No Hit
GCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC19530.21482359825677194No Hit
AGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC16430.18072461440649068No Hit
TCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC16300.17929465701922082No Hit
ACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT16010.17610475207838805No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT15520.17071491269560168No Hit
TGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC13010.14310573544908362No Hit
TGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTTG11450.1259462468018453Illumina Single End Adapter 1 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG11070.1217663713621334No Hit
GGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC10490.11538656148046787No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT10290.11318662703851425No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC10260.11285663687222122No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT9930.10922674504299773No Hit
ATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC9480.10427689254860203No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.10141697777406233No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT9220.10141697777406233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG351.0192525E-746.0000041
GTCTAAA351.0192525E-746.00000432
GCCGATA351.0192525E-746.0000048
AATTCGG351.0192525E-746.0000041
ATGACGG750.046.0000041
ATAACGG351.0192525E-746.0000041
CAGCGAT501.6370905E-1146.020
CCTTACC206.310756E-446.021
GTACCGG405.6097633E-946.01
AGATCGC206.310756E-446.021
ACCGGCC253.416588E-546.020
GCGACAA301.8609717E-646.08
GGTAACG206.310756E-446.044
GGCACTA405.6097633E-946.07
GCAACGC253.416588E-546.012
GTCCTCG253.416588E-546.022
CGTGAAT206.310756E-446.05
GGCCGAT600.046.07
CCAATCG206.310756E-446.023
CACGCGG551.8189894E-1246.01
Produced by FastQC (version 0.11.5)